铜胁迫对黑麦草种子萌发及幼苗生理生态的影响
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摘要
以多年生黑麦草为研究对象,采用植物培育袋对黑麦草进行0,40,80,160,320,640mg/L的重金属Cu2+胁迫处理,通过测定种子萌发率、生物量、根系长度及叶绿素含量,分析了黑麦草对重金属Cu2+的积累特性和生长、生理响应.结果表明:黑麦草种子的发芽率随着铜处理浓度的升高呈现先上升后下降的趋势,低铜浓度对黑麦草根系长度起促进作用,而长时间铜胁迫则对黑麦草根系长度起抑制作用,且胁迫浓度越高抑制作用越强;铜胁迫抑制了黑麦草叶绿素的增长,且抑制作用随着胁迫浓度的增加而增强.实验结果对进一步明确铜对高等植物的伤害机理以及铜污染对草原牧草经济价值的影响具有重要意义,也可为草原牧草选种、草原中铜元素的生态风险评价提供理论依据.
To understand the impact of copper pollution on physiological characteristics and economic importance of grassland plants,the growth and physiological responses,copper uptake and accumulation by Perennial Ryegrass(Lolium perenne L.)were investigated.The plants were subjected to copper stress at 0,40,80,160,320,640 mg/L and germination rate,biomass,root length ratio and chlorophyll content were evaluated.The results showed that the germination rate of ryegrass seeds increases first,and then decreases with the elevation of copper concentration,and lower copper level promotes the root length of ryegrass.However,long-term copper stress reduced the root length and higher copper stress strongly inhibited root length of the plant.The results underscore the influence of copper stress on Perennial Ryegrass and further study is needed to delineate the possible mechanism underlying Cu-induced damage to plant and the role of copper contamination on economic consequences of grassland.Additionally,these results provide a theoretical basis for the ecological risk assessment of grassland pasture selection and copper level in grassland.
To understand the impact of copper pollution on physiological characteristics and economic importance of grassland plants,the growth and physiological responses,copper uptake and accumulation by Perennial Ryegrass(Lolium perenne L.)were investigated.The plants were subjected to copper stress at 0,40,80,160,320,640 mg/L and germination rate,biomass,root length ratio and chlorophyll content were evaluated.The results showed that the germination rate of ryegrass seeds increases first,and then decreases with the elevation of copper concentration,and lower copper level promotes the root length of ryegrass.However,long-term copper stress reduced the root length and higher copper stress strongly inhibited root length of the plant.The results underscore the influence of copper stress on Perennial Ryegrass and further study is needed to delineate the possible mechanism underlying Cu-induced damage to plant and the role of copper contamination on economic consequences of grassland.Additionally,these results provide a theoretical basis for the ecological risk assessment of grassland pasture selection and copper level in grassland.
引文
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[8]RAMOS M C.Metals in vineyard soils of the Peneds area(NE Spain)after compost application[J].Journal of Environmental Management,2006,78:209-215.
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[14]李锋民,熊治廷,胡洪营.海州香薷对铜的蓄积及铜的毒性效应[J].环境科学,2003,24(3):30-34.
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[16]CHEN J R,SHAFI M,LI S,et al.Copper induced oxidative stresses,antioxidant responses and phytoremediation potential of Moso bamboo(Phyllostachys pubescens)[J].Scientific Reports,2015,5(3):13554.
[17]沈伟其.测定水稻叶片叶绿素含量的混合液提取法[J].植物生理学通讯,1988(3):62-64.
[18]王素平,郭世荣,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(2):32-38.
[19]江行玉,赵可夫.植物重金属伤害及其抗性生理[J].应用与环境生物学报,2001,7(1):92-97.
[20]YE N,LI H,ZHU G,et al.Copper suppresses abscisic acid catabolism and catalase activity,and inhibits seed germination of rice[J].Plant&Cell Physiology,2014,55(11):2008-2016.
[21]刘建霞,温日宇,刘建林,等.铜胁迫对板蓝根幼苗生长的影响[J].山西农业科学,2017,45(10):1659-1661.
[22]刘俊祥,魏树强,翟飞飞,等.Cd2+胁迫下多年生黑麦草的生长与生理响应[J].核农学报,2015,29(3):587-594.
[23]SAˇGLAM A,YETI爦SIN F,DEMIRALAY M.Chapter 2-copper stress and responses in plants[J].Plant Metal Interaction,2016:21-40.
[24]ESPOSITO J B N,ESPOSITO B P,AZEVEDO R A,et al.Protective effect of Mn(III)-desferrioxamine B upon xidative stress caused by ozone and acid rain in the Brazilian soybean cultivar Glycine max,“Sambaiba”[J].Environmental Science and Pollution Research,2015,22(7):5315-5324.
[25]JANAS K,TOMASZEWSKA J,RYBACZEK D,et al.The impact of copper ions on growth,ipid peroxidation,and phenolic compound accumulation and localization in lentil(Lens culinaris Medic.)seedlings[J].Journal of Plant Physiology,2010,167:270-276.
[26]THAO N P,KHAN M I,THU N B,et al.Role of ethylene and its cross talk with other signaling molecules in plant responses to heavy metal stress[J].Plant Physiology,2015,169(1):73.
[27]李中阳,樊向阳,宋正国,等.CO2浓度升高对2个品种水稻Cu吸收、根形态和植物络合素合成的影响[J].水土保持学报,2013,27(4):207-211.
[28]MORAVCOV,T7)UMA J,DUˇCAIOVZ K,et al.Influence of salicylic acid pretreatment on seeds germination and some defence mechanisms of Zea mays plants under copper stress[J].Plant Physiology&Biochemistry Ppb,2018,122:19.
[29]BRAHIMA S,JOKEA D,ANN C,et al.Roteome responses of Arabidopsis thaliana exposed to mild cadmium stress[J].Journal of Plant Physiology,2010,167(5):247-254.
[30]RALDUGINA G N,KRASAVINA M S,LUNKOVA N F,et al.Chapter 4-resistance of plants to Cu stress:transgenesis[J].Plant Metal Interaction,2016,69:114-119.
[31]RIC D,VOS C H,SCHAT H,et al.Copper-induced damage to the permeability barrier in roots of Sliene cucubalus[J].Journal of Plant Physiology,1989,135(2):164-169.
[32]YRUELA I,ALFONSO M,BARN M,et al.Copper effect on the protein composition of photosystem II[J].Physiologia Plantarum,2000,110(4):551-557.
[33]薛艳,周东美,郝秀珍,等.两种不同耐性青菜种子萌发和根伸长对铜响应的研究[J].农业环境科学学报,2006,25(5):1107-1110.
[34]甄泉,严密,杨红飞.污染对野艾蒿生长发育的胁迫及伤害[J].生态学报,2006,17(8):1505-1510.
[35]朱成凤,王友保.铜胁迫下红花碧桃组织浸提液对黑麦草种子萌发和幼苗生长的影响[D].芜湖:安徽师范大学,2016.
[36]钟时伟.胁迫条件下植物光合作用机理研究进展[J].园艺与种苗,2018(6):59-62.
[2]KONG Z Y,GLICK B R,DUAN J,et al.Effects of 1-aminocyclopropane-1-carboxylate(ACC)deaminase-overproducing Sinorhizobium meliloti on plant growth and copper tolerance of Medicago lupulina[J].Plant&Soil,2015,391(1):1-16.
[3]公勤,康群,王玲,等.重金属铜对植物毒害机理的研究现状及展望[J].南方农业学报,2018,49(3):469-475.
[4]BAEK S A,HAN T,AHN S K,et al.Effects of heavy metals on plant growths and pigment contents in Arabidopsis thaliana[J].Plant Pathology Jounal,2012,28(4):446-452.
[5]JIN Q,ZHU K,XIE Y,et al.Heme oxygenase-1is involved in ascorbic acid-induced alleviation of cadmium toxicity in root tissues of Medicago sativa[J].Plant Soil,2013,366(1/2):605-616.
[6]YANG Z,CHEN J,DOU R,et al.Assessment of the phytotoxicity of metal oxide nanoparticles on two crop plants,maize(Zea mays L.)and rice(Oryza sativa L.)[J].International Journal of Environmental Research&Public Health,2015,12(12):15100-15109.
[7]WANG Y,HUANG J,GAO Y.Subcellular accumulation of different concentrations of Cadmium,Nickel,and Copper in Indian Mustard and application of a Sigmoidal Model[J].Journal of Environmental Quality,2013,42(4):1142-1150.
[8]RAMOS M C.Metals in vineyard soils of the Peneds area(NE Spain)after compost application[J].Journal of Environmental Management,2006,78:209-215.
[9]杨俊,孙燕,张燕,等.Cu、Pb和As胁迫下4种植物的耐性及富集特征研究[J].环境与发展,2018,30(8):115-117.
[10]胡鹏杰,李柱,钟道旭,等.我国土壤重金属污染植物吸取修复研究进展[J].植物生理学报,2014,50(5):577-584.
[11]马博英.多年生黑麦草的逆境生理研究进展[J].生物学杂志,2011,27(2):58-61.
[12]PFEIFER M,MARTIS M,ASP T,et al.The Perennial Ryegrass genome zipper:targeted use of genome resources for comparative grass genomics[J].Plant Physiology,2013,161(2):571-582.
[13]ALI M B,SINGH N,SHOHAEL A M.Phenolics metabolism and lignin synthesis in root suspension cultures of Panax ginsengin response to copper stress[J].Plant Science,2006,171:147-154.
[14]李锋民,熊治廷,胡洪营.海州香薷对铜的蓄积及铜的毒性效应[J].环境科学,2003,24(3):30-34.
[15]KOLBERT Z,PETA,LEHOTAI N,et al.Long-term copper(Cu2+)exposure impacts on auxin,nitric oxide(NO)metabolism and morphology of Arabidopsis thaliana L[J].Plant Growth Regulation,2012,68(2):151-159.
[16]CHEN J R,SHAFI M,LI S,et al.Copper induced oxidative stresses,antioxidant responses and phytoremediation potential of Moso bamboo(Phyllostachys pubescens)[J].Scientific Reports,2015,5(3):13554.
[17]沈伟其.测定水稻叶片叶绿素含量的混合液提取法[J].植物生理学通讯,1988(3):62-64.
[18]王素平,郭世荣,胡晓辉,等.盐胁迫对黄瓜幼苗叶片光合色素含量的影响[J].江西农业大学学报,2006,28(2):32-38.
[19]江行玉,赵可夫.植物重金属伤害及其抗性生理[J].应用与环境生物学报,2001,7(1):92-97.
[20]YE N,LI H,ZHU G,et al.Copper suppresses abscisic acid catabolism and catalase activity,and inhibits seed germination of rice[J].Plant&Cell Physiology,2014,55(11):2008-2016.
[21]刘建霞,温日宇,刘建林,等.铜胁迫对板蓝根幼苗生长的影响[J].山西农业科学,2017,45(10):1659-1661.
[22]刘俊祥,魏树强,翟飞飞,等.Cd2+胁迫下多年生黑麦草的生长与生理响应[J].核农学报,2015,29(3):587-594.
[23]SAˇGLAM A,YETI爦SIN F,DEMIRALAY M.Chapter 2-copper stress and responses in plants[J].Plant Metal Interaction,2016:21-40.
[24]ESPOSITO J B N,ESPOSITO B P,AZEVEDO R A,et al.Protective effect of Mn(III)-desferrioxamine B upon xidative stress caused by ozone and acid rain in the Brazilian soybean cultivar Glycine max,“Sambaiba”[J].Environmental Science and Pollution Research,2015,22(7):5315-5324.
[25]JANAS K,TOMASZEWSKA J,RYBACZEK D,et al.The impact of copper ions on growth,ipid peroxidation,and phenolic compound accumulation and localization in lentil(Lens culinaris Medic.)seedlings[J].Journal of Plant Physiology,2010,167:270-276.
[26]THAO N P,KHAN M I,THU N B,et al.Role of ethylene and its cross talk with other signaling molecules in plant responses to heavy metal stress[J].Plant Physiology,2015,169(1):73.
[27]李中阳,樊向阳,宋正国,等.CO2浓度升高对2个品种水稻Cu吸收、根形态和植物络合素合成的影响[J].水土保持学报,2013,27(4):207-211.
[28]MORAVCOV,T7)UMA J,DUˇCAIOVZ K,et al.Influence of salicylic acid pretreatment on seeds germination and some defence mechanisms of Zea mays plants under copper stress[J].Plant Physiology&Biochemistry Ppb,2018,122:19.
[29]BRAHIMA S,JOKEA D,ANN C,et al.Roteome responses of Arabidopsis thaliana exposed to mild cadmium stress[J].Journal of Plant Physiology,2010,167(5):247-254.
[30]RALDUGINA G N,KRASAVINA M S,LUNKOVA N F,et al.Chapter 4-resistance of plants to Cu stress:transgenesis[J].Plant Metal Interaction,2016,69:114-119.
[31]RIC D,VOS C H,SCHAT H,et al.Copper-induced damage to the permeability barrier in roots of Sliene cucubalus[J].Journal of Plant Physiology,1989,135(2):164-169.
[32]YRUELA I,ALFONSO M,BARN M,et al.Copper effect on the protein composition of photosystem II[J].Physiologia Plantarum,2000,110(4):551-557.
[33]薛艳,周东美,郝秀珍,等.两种不同耐性青菜种子萌发和根伸长对铜响应的研究[J].农业环境科学学报,2006,25(5):1107-1110.
[34]甄泉,严密,杨红飞.污染对野艾蒿生长发育的胁迫及伤害[J].生态学报,2006,17(8):1505-1510.
[35]朱成凤,王友保.铜胁迫下红花碧桃组织浸提液对黑麦草种子萌发和幼苗生长的影响[D].芜湖:安徽师范大学,2016.
[36]钟时伟.胁迫条件下植物光合作用机理研究进展[J].园艺与种苗,2018(6):59-62.
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