重阳木树皮和叶片挥发油化学成分的GC-MS分析
|
摘要
研究重阳木Bischofia polycarpa树皮和叶片的挥发油化学成分.采用水蒸气蒸馏法提取重阳木树皮和叶片的挥发油,气相色谱-质谱联用(GC-MS)技术分析、鉴定其中化学成分,峰面积归一法分析各个化合物在挥发油中的质量分数.从重阳木树皮挥发油的26个峰中鉴定出18种化合物,主要化学成分为丁香酚(12.45%)、糠醛(10.43%)、十五酸(9.46%)、十七酸乙酯(8.98%)、2-甲氧基-4-乙烯基苯酚(7.51%)等;从重阳木新鲜叶片挥发油的16个峰中鉴定出14种化合物,其中主要成分为1-己醇(26.94%)、十五酸(10.8%)、环己酮(10.63%)、十七酸乙酯(10.2%)、月桂酸(6.11%)等.
The volatile oils from the tree bark and leaf of Bischofia polycarpa were extracted by vapor distillation. Their chemical constituents were separated and identified by GC-MS and the relative content of main compounds in volatile oils were determined by peak area normalization method. The results show that 26 peaks were separated from the tree bark volatile oil of B. polycarpa,and 18 compounds among them were identified. The main compounds were engenol( 12. 45%),furole( 10. 43%),pentadecylic acid( 9. 46%),ethyl heptadecanoate( 8. 98%),4-ethenyl-2-methoxyphenol( 7. 51%). Meanwhile,21 peaks were separated from the tree leaf volatile oil of B.polycarpa,and 15 compounds among them were identified. The main compounds were caproyl alcohol( 26. 94%),pentadecylic acid( 10. 8%),cyclohexanone( 10. 63%),ethyl heptadecanoate( 10. 2%),vulvic acid( 6. 11%).
The volatile oils from the tree bark and leaf of Bischofia polycarpa were extracted by vapor distillation. Their chemical constituents were separated and identified by GC-MS and the relative content of main compounds in volatile oils were determined by peak area normalization method. The results show that 26 peaks were separated from the tree bark volatile oil of B. polycarpa,and 18 compounds among them were identified. The main compounds were engenol( 12. 45%),furole( 10. 43%),pentadecylic acid( 9. 46%),ethyl heptadecanoate( 8. 98%),4-ethenyl-2-methoxyphenol( 7. 51%). Meanwhile,21 peaks were separated from the tree leaf volatile oil of B.polycarpa,and 15 compounds among them were identified. The main compounds were caproyl alcohol( 26. 94%),pentadecylic acid( 10. 8%),cyclohexanone( 10. 63%),ethyl heptadecanoate( 10. 2%),vulvic acid( 6. 11%).
引文
[1]陆支悦,姜卫兵,翁忙玲.重阳木的园林特性及其开发应用[J].江西农业学报,2009,21(6):64-67.
[2]郭振锋.重阳木能源林的培育技术[J].中国林副特产,2011(6):51-53.
[3]周琼,刘路,陈春燕,等.园林害虫重阳木锦斑蛾的生活习性及其防治[C]//雷朝亮,王满穂,赵树英.华中昆虫研究:8卷.武汉:湖北科学技术出版社,2012:361.
[4]周英,耿晓东,顾国海.重阳木锦斑蛾蛹的研究[J].江苏农业科学,2012,40(11):129-130.
[5]吴雪芬,韩鹰,田松青.重阳木斑蛾生物学特性观察及综合防治技术[J].安徽农业科学,2007,35(35):11396-11398.
[6]戴凤凤.重阳木斑蛾的初步观察[J].江西林业科技,1983(4):21-22.
[7]PROFFIT M,BIRGERSSON G,BENGTSSON M,et al.Attraction and oviposition of Tuta absoluta females in response to tomato leaf volatiles[J].J Chem Ecol,2011,37(6):565-574.
[8]PINERO J C,DORN S.Response of female oriental fruit moth to volatiles from apple and peach trees at three phenological stages[J].Entomol Exp App,2009,131(8):67-74.
[9]SOLE J,SANS A,RIBA M,et al.Behavioural and electrophysiological responses of the European corn borer Ostrinia nubilalis to host-plant volatiles and related chemicals[J].Physiol Entomol,2010,35(4):354-363.
[10]尤克西,周琼,荆奇.柑橘大实蝇成虫的取食、交配和产卵行为[J].湖南师范大学自然科学学报,2012,35(3):68-71.
[11]SCHMIDT-BSSER D,von ARX M,GUERIN P M.Host plant volatiles serve to increase the response of male European grape berry moths,Eupoecilia ambiguella,to their sex pheromone[J].J Comp Physiol A,2009,195(9):853-864.
[12]SYED Z,GUERIN P M.Tsetse flies are attracted to the invasive plant Lantana camara[J].J Insect Physiol,2004,50(1):43-50.
[13]COUTY A,van EMDEN H,PERRY J N,et al.The roles of olfaction and vision in host-plant finding by the diamondback moth,Plutella xylostella[J].Physiol Entomol,2006,31(2):134-145.
[14]闫凤鸣.化学生态学[M].北京:科学出版社,2003.
[15]赵冬香,高景林,陈宗懋.植食性昆虫对寄主植物的定向行为研究进展[J].热带农业科学,2004,24(2):62-67.
[16]DICHENS J C,PRESTWICH G D,NG C,et al.Selectively fluorinated analogs reveal differential olfactory reception and inactivation of green leaf volatiles in insects[J].J Chem Ecol,1993,19(9):1981-1991.
[17]MLLER C,HILKER M.The effect of a green leaf volatile on host plant finding by larvae of a herbivorous insect[J].Naturwissenschaften,2000,87(5):216-219.
[18]ALLARD A C,ROBERT J B,BRUCE W Z,et al.Behaviorally active green leaf volatiles for monitoring the leaf beetle,diorhabda elongata,a biocontrol agent of saltcedar,tamarix spp[J].J Chem Ecol,2006,32(12):2695-2708.
[19]LIVY WLLL,JACQUELYN L B,CESAR R S,et al.Plant volatiles influence electrophysiological and behavioral responses of Lygus hesperus.[J].J Chem Ecol,2010,36(5):467-478.
[20]LADD T L.Japanese beetle:enhancement of lures by eugenol and caproic acid[J].J Econom Entom,1980,73(5):718-720.
[21]郭丽.桑树对桑天牛引诱机制的研究[D].保定:河北农业大学,2006.
[22]安靖靖.烟草甲对植物源调味料及其挥发物的行为反应研究[D].郑州:河南农业大学,2010.
[23]李英华,袁海英,巴吐尔,等.新疆草莓采后主要致腐病原真菌的鉴定及正己醇处理对其抑制作用[J].新疆农业大学学报,2008,32(1):60-63.
[24]李英华,袁海英,张辉,等.采后正己醇处理对草莓果实活性氧代谢和衰老的影响[J].食品科学,2010,31(4):272-275.
[2]郭振锋.重阳木能源林的培育技术[J].中国林副特产,2011(6):51-53.
[3]周琼,刘路,陈春燕,等.园林害虫重阳木锦斑蛾的生活习性及其防治[C]//雷朝亮,王满穂,赵树英.华中昆虫研究:8卷.武汉:湖北科学技术出版社,2012:361.
[4]周英,耿晓东,顾国海.重阳木锦斑蛾蛹的研究[J].江苏农业科学,2012,40(11):129-130.
[5]吴雪芬,韩鹰,田松青.重阳木斑蛾生物学特性观察及综合防治技术[J].安徽农业科学,2007,35(35):11396-11398.
[6]戴凤凤.重阳木斑蛾的初步观察[J].江西林业科技,1983(4):21-22.
[7]PROFFIT M,BIRGERSSON G,BENGTSSON M,et al.Attraction and oviposition of Tuta absoluta females in response to tomato leaf volatiles[J].J Chem Ecol,2011,37(6):565-574.
[8]PINERO J C,DORN S.Response of female oriental fruit moth to volatiles from apple and peach trees at three phenological stages[J].Entomol Exp App,2009,131(8):67-74.
[9]SOLE J,SANS A,RIBA M,et al.Behavioural and electrophysiological responses of the European corn borer Ostrinia nubilalis to host-plant volatiles and related chemicals[J].Physiol Entomol,2010,35(4):354-363.
[10]尤克西,周琼,荆奇.柑橘大实蝇成虫的取食、交配和产卵行为[J].湖南师范大学自然科学学报,2012,35(3):68-71.
[11]SCHMIDT-BSSER D,von ARX M,GUERIN P M.Host plant volatiles serve to increase the response of male European grape berry moths,Eupoecilia ambiguella,to their sex pheromone[J].J Comp Physiol A,2009,195(9):853-864.
[12]SYED Z,GUERIN P M.Tsetse flies are attracted to the invasive plant Lantana camara[J].J Insect Physiol,2004,50(1):43-50.
[13]COUTY A,van EMDEN H,PERRY J N,et al.The roles of olfaction and vision in host-plant finding by the diamondback moth,Plutella xylostella[J].Physiol Entomol,2006,31(2):134-145.
[14]闫凤鸣.化学生态学[M].北京:科学出版社,2003.
[15]赵冬香,高景林,陈宗懋.植食性昆虫对寄主植物的定向行为研究进展[J].热带农业科学,2004,24(2):62-67.
[16]DICHENS J C,PRESTWICH G D,NG C,et al.Selectively fluorinated analogs reveal differential olfactory reception and inactivation of green leaf volatiles in insects[J].J Chem Ecol,1993,19(9):1981-1991.
[17]MLLER C,HILKER M.The effect of a green leaf volatile on host plant finding by larvae of a herbivorous insect[J].Naturwissenschaften,2000,87(5):216-219.
[18]ALLARD A C,ROBERT J B,BRUCE W Z,et al.Behaviorally active green leaf volatiles for monitoring the leaf beetle,diorhabda elongata,a biocontrol agent of saltcedar,tamarix spp[J].J Chem Ecol,2006,32(12):2695-2708.
[19]LIVY WLLL,JACQUELYN L B,CESAR R S,et al.Plant volatiles influence electrophysiological and behavioral responses of Lygus hesperus.[J].J Chem Ecol,2010,36(5):467-478.
[20]LADD T L.Japanese beetle:enhancement of lures by eugenol and caproic acid[J].J Econom Entom,1980,73(5):718-720.
[21]郭丽.桑树对桑天牛引诱机制的研究[D].保定:河北农业大学,2006.
[22]安靖靖.烟草甲对植物源调味料及其挥发物的行为反应研究[D].郑州:河南农业大学,2010.
[23]李英华,袁海英,巴吐尔,等.新疆草莓采后主要致腐病原真菌的鉴定及正己醇处理对其抑制作用[J].新疆农业大学学报,2008,32(1):60-63.
[24]李英华,袁海英,张辉,等.采后正己醇处理对草莓果实活性氧代谢和衰老的影响[J].食品科学,2010,31(4):272-275.