野生黄花蒿土壤特征对药用次生代谢产物的影响
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摘要
为了明确影响黄花蒿药用次生代谢产物累积的土壤特征因素,测定贵州省三穗县和剑河县野生黄花蒿的总多酚、东莨菪内酯、猫眼草酚D、猫眼草黄素、青蒿素、青蒿酸含量,以及土壤养分含量、pH值、土壤酶活性,利用SPSS软件对土壤特征与黄花蒿药用次生代谢产物含量进行相关性分析,探讨土壤特征对黄花蒿药用次生代谢产物的影响。结果表明,不同海拔不同样地黄花蒿的药用次生代谢产物含量差异显著。根际与非根际土壤的理化性质大多存在差异。野生黄花蒿样地中土壤蔗糖酶活性较高,土壤脲酶和磷酸酶活性相对较低。土壤脲酶、磷酸酶、蔗糖酶活性,碱解氮含量与青蒿素、青蒿酸含量呈显著或极显著正相关,全钾含量和pH值与青蒿素、青蒿酸含量呈显著或极显著负相关,土壤有机质、全磷、全氮、速效钾含量与青蒿酸含量呈显著或极显著正相关,土壤有效磷含量与总多酚、猫眼草酚D和青蒿素含量呈显著或极显著负相关。综上,提高土壤脲酶、磷酸酶、蔗糖酶活性能够提高黄花蒿的青蒿素、青蒿酸含量,提高土壤有机质、全氮、全磷、碱解氮和速效钾含量可以提高黄花蒿的青蒿酸含量,降低土壤全钾、有效磷含量和p H值有利于黄花蒿药用次生代谢产物的积累。
To clarify the soil properties factors affecting the concentrations of secondary metabolites of Artemisia annua L.,the contents of total polyphenols,scopoletin,chrysosplenol-D,chrysosplenetin,artemisinin,artemisic acid of Artemisia annua L. and soil nutrients contents,p H,soil enzyme activities were analyzed in Sansui county and Jianhe county,Guizhou province,and the correlation analysis among them was made to study the effects of soil properties on the secondary metabolites of A. annua. The results showed that the contents of medicinal secondary metabolites of A. annua displayed significant difference at different altitudes and sample points. Most of the basic physicochemical property of rhizosphere and no-rhizosphere soils displayed significant difference. Soil sucrase activity was higher,and soil urease and phosphatase activities were relatively low in wild A. annua sample points. The activities of sucrase,urease,phosphatase and alkaline nitrogen content were significantly or extremely significantly positively related to the contents of artemisinin and artemisic acid while the total potassium content and p H were opposite. The contents of soil organic matter,total phosphorus,total nitrogen and available potassium were significantly or extremely significantly positively related to artemisic acid contents,while the soil available phosphoruscontent was significantly or extremely significantly negatively related to the contents of polyphenols,chrysosplenol-D and artemisinin. In conclusion,the contents of artemisinin and artemisic acid could be increased by increasing the activities of soil urease,phosphatase and sucrase. Increasing the contents of soil organic matter,total nitrogen,total phosphorus,available nitrogen and available potassium could increase the contents of artemisic acid. Reducing the contents of soil total potassium,available phosphorus and p H was beneficial to the accumulation of medicinal secondary metabolites of A. annua.
To clarify the soil properties factors affecting the concentrations of secondary metabolites of Artemisia annua L.,the contents of total polyphenols,scopoletin,chrysosplenol-D,chrysosplenetin,artemisinin,artemisic acid of Artemisia annua L. and soil nutrients contents,p H,soil enzyme activities were analyzed in Sansui county and Jianhe county,Guizhou province,and the correlation analysis among them was made to study the effects of soil properties on the secondary metabolites of A. annua. The results showed that the contents of medicinal secondary metabolites of A. annua displayed significant difference at different altitudes and sample points. Most of the basic physicochemical property of rhizosphere and no-rhizosphere soils displayed significant difference. Soil sucrase activity was higher,and soil urease and phosphatase activities were relatively low in wild A. annua sample points. The activities of sucrase,urease,phosphatase and alkaline nitrogen content were significantly or extremely significantly positively related to the contents of artemisinin and artemisic acid while the total potassium content and p H were opposite. The contents of soil organic matter,total phosphorus,total nitrogen and available potassium were significantly or extremely significantly positively related to artemisic acid contents,while the soil available phosphoruscontent was significantly or extremely significantly negatively related to the contents of polyphenols,chrysosplenol-D and artemisinin. In conclusion,the contents of artemisinin and artemisic acid could be increased by increasing the activities of soil urease,phosphatase and sucrase. Increasing the contents of soil organic matter,total nitrogen,total phosphorus,available nitrogen and available potassium could increase the contents of artemisic acid. Reducing the contents of soil total potassium,available phosphorus and p H was beneficial to the accumulation of medicinal secondary metabolites of A. annua.
引文
[1] LI T. Using hairy roots for production of valuable plant secondary metabolites[J]. Advances in Biochemical Engineering Biotechnology,2015,49:275.
[2]陈晓亚,刘培,孟玉玲,等.植物的次生代谢[J].科学,1996(5):37-39.
[3]苏文华,张光飞,李秀华,等.植物药材次生代谢产物的积累与环境的关系[J].中草药,2005,36(9):1415-1418.
[4]王玉明,李锦,张丽媛,等.药用植物次生代谢产物积累规律的研究概况[J].中南药学,2012,10(2):136-139.
[5] XU Z C,JI A J,ZHANG X,et al. Biosynthesis and regulation of active compounds in medicinal model plant Salvia miltiorrhiza[J]. Chinese Herbal Medicines,2016,8(1):3-11.
[6]国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015:198.
[7]林油润.中国植物志[M].北京:科学出版社,1991,76(2):62-65.
[8] TU Y Y. The discovery of artemisinin(qinghaosu)and gifts from Chinese medicine[J]. Nature Medicine,2011,17(10):1217-1220.
[9]汪猷,夏志强,周凤仪,等.青蒿素生物合成的研究Ⅲ.青蒿素和青蒿素B生物合成中的关键性中间体:青蒿酸[J].化学学报,1988,46(11):1152-1153.
[10] FERREIRA J F,LUTHRIA D L,SASAKI T,et al. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer[J]. Molecules,2010,15(5):3135-3170.
[11] BHAKUNI R S,JAIN D C,SHARMA R P,et al. Secondary metabolites of Artemisia annua and their biological activity[J]. Current Science,2001,80(1):35-48.
[12]邸胜娟,罗世琼,杨占南,等.土壤特征对黄花蒿酚类物质的影响[J].江苏农业科学,2017,45(11):85-89.
[13]彭锐,王远会,马鹏,等.黄花蒿中青蒿素含量与产地气象因子及土壤的关系研究[J].西南农业学报,2010,23(2):519-522.
[14]邸胜娟.黄花蒿(Artemisia annua L.)抗疟相关成分累积与土壤特征[D].贵阳:贵州师范大学,2017.
[15]李锋,韦霄,许成琼,等.广西黄花蒿类型调查研究[J].广西植物,1997,17(3):231-234.
[16]王满莲,韦霄,蒋运生,等.不同土壤环境对黄花蒿生长和青蒿素含量的影响研究[J].植物研究,2010,30(4):424-427.
[17]张金燕,孙雪婷,陈军文,等.连作三七根际土壤化感物质检测及其提取液对三种作物种子萌发的影响[J].南方农业学报,2017,48(7):1178-1184.
[18] HAWKES C V,WREN I F,HERMAN D J,et al. Plant invasion alters nitrogen cycling by modifying the soil nitrifying community[J]. Ecology Letters,2005,8(9):976-985.
[19]刘菊梅,曹博,石春芳,等.紫花苜蓿根际效应对河套灌区土壤盐分和养分的影响[J].南方农业学报,2018,49(2):246-252.
[20]罗世琼,黄建国,袁玲.野生黄花蒿土壤的养分状况与微生物特征[J].土壤学报,2014,51(4):868-879.
[21]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000:13-18.
[22]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[23] JESSING K K,CEDERGREEN N,JENSEN J,et al. Degradation and ecotoxicity of the biomedical drug artemisinin in soil[J]. Environmental Toxicology&Chemistry,2009,28(4):701-710.
[24] MARTNEZ-BLANCO J,ANTN A,RIERADEVALL J,et al. Comparing nutritional value and yield as functional units in the environmental assessment of horticultural production with organic or mineral fertilization[J]. International Journal of Life Cycle Assessment,2011,16(1):12-26.
[25]余正文,王伯初,杨占南,等.高效液相色谱法同时测定青蒿中东莨菪内酯、猫眼草酚及猫眼草黄素[J].中国酿造,2011,30(9):175-178.
[26]余正文.青蒿抗疟化合物代谢积累及其相关性研究[D].重庆:重庆大学,2010.
[27]万忠梅,宋长春.小叶章湿地土壤酶活性分布特征及其与活性有机碳表征指标的关系[J].湿地科学,2008,6(2):249-257.
[28]马丽.豫东不同树龄梨园土壤微生物生态特征[J].河南农业科学,2018,47(1):37-42.
[29]王楠,徐俊平,赵志祎,等.氮素形态配比对添加玉米秸秆白浆土微生物学特性的影响[J].河南农业科学,2017,46(6):56-61.
[30]刘建新.不同农田土壤酶活性与土壤养分相关关系研究[J].土壤通报,2004,35(4):523-525.
[31] STADDON W J,DUCHESNE L C,TREVORS J T. Acid phosphatase,alkaline phosphatase and arylsulfatase activities in soils from a jack pine(Pinus banksiana Lamb.)ecosystem after clear-cutting,prescribed burning,and scarificaion[J]. Biology&Fertility of Soils,1998,27(1):1-4.
[32]罗世琼.黄花蒿土壤微生物与抗疟相关成分的关联性研究[D].重庆:西南大学,2013.
[33]胡雷,王长庭,王根绪,等.三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化[J].草业学报,2014,23(3):8-19.
[34]李锦馨,张阁,马燕,等. UV-B辐射胁迫对药用植物次生代谢产物的影响研究进展[J].河南农业科学,2018,47(5):1-7.
[35]张慢慢,邵惠芳,许自成,等.烤烟产量与气候因子的关系分析[J].河南农业科学,2016,45(7):34-38.
[2]陈晓亚,刘培,孟玉玲,等.植物的次生代谢[J].科学,1996(5):37-39.
[3]苏文华,张光飞,李秀华,等.植物药材次生代谢产物的积累与环境的关系[J].中草药,2005,36(9):1415-1418.
[4]王玉明,李锦,张丽媛,等.药用植物次生代谢产物积累规律的研究概况[J].中南药学,2012,10(2):136-139.
[5] XU Z C,JI A J,ZHANG X,et al. Biosynthesis and regulation of active compounds in medicinal model plant Salvia miltiorrhiza[J]. Chinese Herbal Medicines,2016,8(1):3-11.
[6]国家药典委员会.中华人民共和国药典一部[M].北京:中国医药科技出版社,2015:198.
[7]林油润.中国植物志[M].北京:科学出版社,1991,76(2):62-65.
[8] TU Y Y. The discovery of artemisinin(qinghaosu)and gifts from Chinese medicine[J]. Nature Medicine,2011,17(10):1217-1220.
[9]汪猷,夏志强,周凤仪,等.青蒿素生物合成的研究Ⅲ.青蒿素和青蒿素B生物合成中的关键性中间体:青蒿酸[J].化学学报,1988,46(11):1152-1153.
[10] FERREIRA J F,LUTHRIA D L,SASAKI T,et al. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer[J]. Molecules,2010,15(5):3135-3170.
[11] BHAKUNI R S,JAIN D C,SHARMA R P,et al. Secondary metabolites of Artemisia annua and their biological activity[J]. Current Science,2001,80(1):35-48.
[12]邸胜娟,罗世琼,杨占南,等.土壤特征对黄花蒿酚类物质的影响[J].江苏农业科学,2017,45(11):85-89.
[13]彭锐,王远会,马鹏,等.黄花蒿中青蒿素含量与产地气象因子及土壤的关系研究[J].西南农业学报,2010,23(2):519-522.
[14]邸胜娟.黄花蒿(Artemisia annua L.)抗疟相关成分累积与土壤特征[D].贵阳:贵州师范大学,2017.
[15]李锋,韦霄,许成琼,等.广西黄花蒿类型调查研究[J].广西植物,1997,17(3):231-234.
[16]王满莲,韦霄,蒋运生,等.不同土壤环境对黄花蒿生长和青蒿素含量的影响研究[J].植物研究,2010,30(4):424-427.
[17]张金燕,孙雪婷,陈军文,等.连作三七根际土壤化感物质检测及其提取液对三种作物种子萌发的影响[J].南方农业学报,2017,48(7):1178-1184.
[18] HAWKES C V,WREN I F,HERMAN D J,et al. Plant invasion alters nitrogen cycling by modifying the soil nitrifying community[J]. Ecology Letters,2005,8(9):976-985.
[19]刘菊梅,曹博,石春芳,等.紫花苜蓿根际效应对河套灌区土壤盐分和养分的影响[J].南方农业学报,2018,49(2):246-252.
[20]罗世琼,黄建国,袁玲.野生黄花蒿土壤的养分状况与微生物特征[J].土壤学报,2014,51(4):868-879.
[21]鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000:13-18.
[22]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986.
[23] JESSING K K,CEDERGREEN N,JENSEN J,et al. Degradation and ecotoxicity of the biomedical drug artemisinin in soil[J]. Environmental Toxicology&Chemistry,2009,28(4):701-710.
[24] MARTNEZ-BLANCO J,ANTN A,RIERADEVALL J,et al. Comparing nutritional value and yield as functional units in the environmental assessment of horticultural production with organic or mineral fertilization[J]. International Journal of Life Cycle Assessment,2011,16(1):12-26.
[25]余正文,王伯初,杨占南,等.高效液相色谱法同时测定青蒿中东莨菪内酯、猫眼草酚及猫眼草黄素[J].中国酿造,2011,30(9):175-178.
[26]余正文.青蒿抗疟化合物代谢积累及其相关性研究[D].重庆:重庆大学,2010.
[27]万忠梅,宋长春.小叶章湿地土壤酶活性分布特征及其与活性有机碳表征指标的关系[J].湿地科学,2008,6(2):249-257.
[28]马丽.豫东不同树龄梨园土壤微生物生态特征[J].河南农业科学,2018,47(1):37-42.
[29]王楠,徐俊平,赵志祎,等.氮素形态配比对添加玉米秸秆白浆土微生物学特性的影响[J].河南农业科学,2017,46(6):56-61.
[30]刘建新.不同农田土壤酶活性与土壤养分相关关系研究[J].土壤通报,2004,35(4):523-525.
[31] STADDON W J,DUCHESNE L C,TREVORS J T. Acid phosphatase,alkaline phosphatase and arylsulfatase activities in soils from a jack pine(Pinus banksiana Lamb.)ecosystem after clear-cutting,prescribed burning,and scarificaion[J]. Biology&Fertility of Soils,1998,27(1):1-4.
[32]罗世琼.黄花蒿土壤微生物与抗疟相关成分的关联性研究[D].重庆:西南大学,2013.
[33]胡雷,王长庭,王根绪,等.三江源区不同退化演替阶段高寒草甸土壤酶活性和微生物群落结构的变化[J].草业学报,2014,23(3):8-19.
[34]李锦馨,张阁,马燕,等. UV-B辐射胁迫对药用植物次生代谢产物的影响研究进展[J].河南农业科学,2018,47(5):1-7.
[35]张慢慢,邵惠芳,许自成,等.烤烟产量与气候因子的关系分析[J].河南农业科学,2016,45(7):34-38.