不同地理种源红松抗虫性及其与次生代谢产物相关性
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摘要
调查不同地理种源红松Pinus koraiensis的球果被害率,以及主要害虫球果尺蛾Eupithecia abietaria和果梢斑螟Dioryctria pryeri的虫口比例。按虫期与被害部位,选取南亚、中亚和北亚3个地理种源的红松,分别测定健康与被害红松韧皮部和球果的单宁、总酚和黄酮含量,分析次生代谢产物与其抗虫性的相关性。结果表明,中亚的红松球果被害率显著高于南亚和北亚种源,其球果中球果尺蛾和果梢斑螟危害率与后2个种源的差异显著;3个种源健康样本韧皮部与球果的单宁和黄酮含量差异显著;受害后3种次生代谢物质含量均呈上升趋势;健康红松不同部位3种次生代谢产物均与其被害率呈负相关,其中韧皮部的单宁含量、球果的单宁和黄酮含量在0.05水平与其被害率显著相关。
Ratio of main pest species Eupithecia abietaria and Dioryctria pryeri and cone damage rate of Pinus koraiensis in Lushuihe seed orchard were investigated.Content of tannin,total phenols and flavonoids of phloem and cone of healthy and host trees from South Asia,Central Asia and Northern Asia were measured according to time and position of damage.The research discussed content difference of tannin,total phenols and flavonoids from three different geographic sources and correlational analyses between three secondary metabolites and insect resistance.The result showed that the damage rate of P.koraiensis cones in Central Asia was significantly higher than that in South Asia and North Asia,in which hazard ratio of two pest species in Central Asia had significant difference with others.The content of tannin,total phenols and flavonoids appeared to be an increasing trend when samples were affected.There was negative correlation between three secondary metabolites and insect resistance from different parts of healthy pine trees,the damaged tree rate had significantly passive correlation with the content of tannin of phloem and the content of tannin and flavonoids of cone at the level of P<0.05.
Ratio of main pest species Eupithecia abietaria and Dioryctria pryeri and cone damage rate of Pinus koraiensis in Lushuihe seed orchard were investigated.Content of tannin,total phenols and flavonoids of phloem and cone of healthy and host trees from South Asia,Central Asia and Northern Asia were measured according to time and position of damage.The research discussed content difference of tannin,total phenols and flavonoids from three different geographic sources and correlational analyses between three secondary metabolites and insect resistance.The result showed that the damage rate of P.koraiensis cones in Central Asia was significantly higher than that in South Asia and North Asia,in which hazard ratio of two pest species in Central Asia had significant difference with others.The content of tannin,total phenols and flavonoids appeared to be an increasing trend when samples were affected.There was negative correlation between three secondary metabolites and insect resistance from different parts of healthy pine trees,the damaged tree rate had significantly passive correlation with the content of tannin of phloem and the content of tannin and flavonoids of cone at the level of P<0.05.
引文
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[12] 王伟,徐建民,李光友,等.桉树枝瘿姬小蜂危害对桉树次生代谢产物含量的影响[J].热带亚热带植物学报,2013,21(6):521-528.
[13] 郑宏.松树营养物质及次生代谢物质含量与思茅松毛虫危害关系的判别分析[J].生物灾害科学,2013,36(1):45-52.
[14] 张思禄.粒肩天牛危害对杨树黄酮含量的影响[J].生物灾害科学,2013,36(3):319-323.
[2] 林凤敏,吴敌,陆宴辉,等.棉花主要抗虫次生物质与其对绿盲蝽抗性的关系[J].植物保护学报,2011,38(3):202-208.
[3] 张立华,林益明,叶功富.施肥对短枝木麻黄幼苗总酚和可溶性缩合单宁含量的影响[J].应用生态学报,2010,21(8):1959-1966.
[4] 鲁艳辉,高广春,郑许松,等.诱集植物香根草对二化螟幼虫致死的作用机制[J].中国农业科学,2017,50(3):486-495.
[5] VERMERRIS W,NICHOLSON R. Phenolic compound biochemistry[M]. Dordrecht,The Netherlands:Springer,2006.
[6] ABD EL-RAHMAN S S,MAZEN M M,MOHAMED H I,et al. Induction of defence related enzymes and phenolic compounds in lupin (Lupinus albus L.) and their effects on host resistance against Fusarium wilt[J]. European Journal of Plant Pathology,2012,134(1):105-116.
[7] 武予清,郭予元.棉花单宁-黄酮类化合物对棉铃虫的抗性潜力[J].生态学报,2001(2):86-89.
[8] 武予清,郭予元.棉花植株中的单宁测定方法研究[J].应用生态学报,2000(2):243-245.
[9] 张军,杨庆凯,王守义,等.大豆抗SCN3过程中总酚含量动态分析[J].大豆科学,2002,21(1):71-74.
[10] 周荣琪.竹叶提取物总黄酮含量测定方法的改进[J].食品科技,2005(7):76-79.
[11] 朱苗苗,杨璐,朱晓峰,等.杏树次生代谢物质含量与小蠹虫危害的关系[J].西北农业学报,2014,23(7):155-159.
[12] 王伟,徐建民,李光友,等.桉树枝瘿姬小蜂危害对桉树次生代谢产物含量的影响[J].热带亚热带植物学报,2013,21(6):521-528.
[13] 郑宏.松树营养物质及次生代谢物质含量与思茅松毛虫危害关系的判别分析[J].生物灾害科学,2013,36(1):45-52.
[14] 张思禄.粒肩天牛危害对杨树黄酮含量的影响[J].生物灾害科学,2013,36(3):319-323.