Plackett-Burman设计联用星点设计-响应面法优选大青叶中靛玉红的提取工艺
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摘要
目的运用Plackett-Burman实验设计联用星点设计-响应面法(CCD-RSM)筛选超声-微波协同提取大青叶中靛玉红的提取工艺。方法运用Plackett-Burman实验设计筛选主要影响因素,采用CCD-RSM优选靛玉红的提取工艺。以乙醇体积分数、料液比、提取时间为自变量,靛玉红提取量为因变量,通过对自变量与因变量的完全2次响应曲面的回归拟合,利用三维曲面图直观分析大青叶中靛玉红提取最佳工艺,并进行预测分析。结果靛玉红的最佳提取工艺为乙醇体积分数为62%,液料比为26,提取时间为9 min。在此最佳条件下,大青叶中靛玉红提取量的最大估计值为4.37 mg/g,实验结果与模型预测值相符。结论利用Plackett-Burman实验设计联用CCD-RSM确定了大青叶中靛玉红的提取工艺,该方法简便,精度更高、重现性好、预测性强。
Objective To investigate the significance of each influencing factor and optimize the process of extracting indirubin from Isatidis Folium by Plackett-Burman design combined with central composite design-response surface methodology(CCD-RSM). Methods Plackett-Burman experimental design was used to screen the main influencing factors, and CCD-RSM was used to optimize the extraction process of indirubin. With the concentration of ethanol, the ratio of material to liquid, and the extraction time as independent variables and the extraction amount of indirubin as dependent variable, the optimum extraction process of indirubin from Isatidis Folium was predicted and analyzed by multiple linear regression and binomial fitting models with independent and dependent variables and the three-dimensional surface graph. Results The optimal extraction process of indirubin was as follows: ethanol concentration 62%, solvent/sample ratio of 26, and extraction time 9 min. Under these conditions, the maximal extraction rate of indirubin was 4.37 mg/g which was consistent with model predictions. Conclusion The optimal process is simple and convenient for extracting indirubin from Isatidis Folium with high precision, reproducibility, and predictability.
Objective To investigate the significance of each influencing factor and optimize the process of extracting indirubin from Isatidis Folium by Plackett-Burman design combined with central composite design-response surface methodology(CCD-RSM). Methods Plackett-Burman experimental design was used to screen the main influencing factors, and CCD-RSM was used to optimize the extraction process of indirubin. With the concentration of ethanol, the ratio of material to liquid, and the extraction time as independent variables and the extraction amount of indirubin as dependent variable, the optimum extraction process of indirubin from Isatidis Folium was predicted and analyzed by multiple linear regression and binomial fitting models with independent and dependent variables and the three-dimensional surface graph. Results The optimal extraction process of indirubin was as follows: ethanol concentration 62%, solvent/sample ratio of 26, and extraction time 9 min. Under these conditions, the maximal extraction rate of indirubin was 4.37 mg/g which was consistent with model predictions. Conclusion The optimal process is simple and convenient for extracting indirubin from Isatidis Folium with high precision, reproducibility, and predictability.
引文
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[16]谭玉柱,童婷婷,谢运飞,等.Plackett-Burman实验设计联用星点设计效应面法优选大黄地上部位蒽醌类成分提取工艺[J].中国药学杂志,2012,47(6):410-414.
[2]于喆源,王勤,宋玉霞.Box-Behnken模型响应面法优化大青叶药材中靛蓝的提取条件[J].河西学院学报,2018,34(5):32-38.
[3]Zhao G H,Li T,Qu X Y,et al.Optimization of ultrasound-assisted extraction of indigo and indirubin from Isatis indigotica Fort.and their antioxidant capacities[J].Food Sci Biotechnol,2017,26(5):1313-1323.
[4]高慧娟,刘志芳,李彩霞,等.双向固体发酵对板蓝根和大青叶抑菌的增效作用初探[J].中草药,2016,47(18):3187-3192.
[5]刘丽娟,王允亮,许树青,等.靛玉红、色胺酮对LPS诱导RAW264.7炎症细胞模型的抗炎作用研究[J].世界中西医结合杂志,2015,10(8):1069-1072.
[6]解光艳,程惠娟,汪长中,等.靛玉红、蛇床子素抗外阴阴道念珠菌病混合菌生物膜作用的研究[J].中国微生态学杂志,2012,24(6):493-496.
[7]赖金伦,刘玉辉,刘畅,等.中药靛玉红作用机理及其临床应用研究进展[J].中兽医医药杂志,2017,36(1):76-79.
[8]张莹莹,刘伟,周文杰,等.少腹逐瘀凝胶贴膏剂基质处方优化及体外透皮特性研究[J].中草药,2018,49(18):4284-4292.
[9]吴伟,崔光华.星点设计-效应面优化法及其在药学中的应用[J].国外医学:药学分册,2000,27(1):292-298.
[10]霍慧君,钟凌云.星点设计-效应面法优选江西樟帮厚朴炮制工艺[J].中成药,2012,34(1):105-108.
[11]蔡恩博,张崇禧,朱启光,等.星点设计-效应面法优选暴马丁香树皮中紫丁香苷提取工艺[J].药物分析杂志,2011,31(9):1808-1811.
[12]胡玉飘,崔秀明,张泽君,等.失效模式分析和星点设计-效应面法优化熟三七皂苷类成分纯化工艺[J].中草药,2018,49(13):3009-3016.
[13]钟杨生,陈恒文,陈芳艳,等.超声-微波协同萃取法提取桑叶γ-氨基丁酸的工艺研究[J].广东农业科学,2018,45(8):107-112.
[14]Wu Z J,Ruan H S,Wang Y H,et al.Optimization of microwave-assisted extraction of puerarin from Radix Puerariae using response surface methodology[J].Sep Sci Technol,2013,48:1657-1664.
[15]程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(4):38-42.
[16]谭玉柱,童婷婷,谢运飞,等.Plackett-Burman实验设计联用星点设计效应面法优选大黄地上部位蒽醌类成分提取工艺[J].中国药学杂志,2012,47(6):410-414.