MTHFR和ERα基因多态性与产后抑郁症易感性的相关研究
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摘要
目的:研究产后抑郁症患者亚甲基四氢叶酸还原酶(MTHFR)和雌激素受体α(ERα)基因多态性的表达,分析其与产后抑郁症易感性的关系。方法:应用聚合酶链式反应和限制性片段长度多态性技术(PCR-RFLP)测定80例产后抑郁症患者(观察组)和80例健康产妇(对照组)的MTHFR和ERα基因型,进而分析各基因型和等位基因与产后抑郁症发病的相关性。结果:观察组MTHFR基因TT基因型和T等位基因频率明显高于对照组,差异有统计学意义(P<0.05)。观察组ERα基因PvuⅡ酶切及XbaⅠ酶切各基因型频率与对照组相比差异无统计学意义(P>0.05),但观察组ERα基因PvuⅡ酶切P等位基因频率高于对照组,差异有统计学意义(P<0.05)。结论:MTHFR基因多态性T等位基因及ERα基因多态性P等位基因可能与产后抑郁症易感性有关。
Objective:To study the expression of methylenetetrahydrofolate reductase(MTHFR)gene and estrogen receptoer alpha(ERα)gene polymorphism,analyze its relations with postpartum depression susceptibility.Methods:MTHFR gene and ERαgene were studied in 80 postpartun depression patients(experimental group)and 80 healthy womem in puerperium(control group)by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).Analyzed the correlation between genotype,allele and postpartum depression.Results:The genotype frequency of TT and the allele frequency of T of MTHFR gene in experimental group were significant higher than that in control group(P<0.05).There were no significant differences in ERα-Pvu Ⅱ genotypes and ERα-Xba Ⅰgenotypes between experimental group and control group(P>0.05),but the allele frequency of P in experimental group was higher than that in control group(P<0.05).Conclusion:The T allele of MTHFR gene polymorphism and the P allele of ERα-PvuⅡ gene polymorphism may be associated with postpartum depression susceptibility.
Objective:To study the expression of methylenetetrahydrofolate reductase(MTHFR)gene and estrogen receptoer alpha(ERα)gene polymorphism,analyze its relations with postpartum depression susceptibility.Methods:MTHFR gene and ERαgene were studied in 80 postpartun depression patients(experimental group)and 80 healthy womem in puerperium(control group)by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).Analyzed the correlation between genotype,allele and postpartum depression.Results:The genotype frequency of TT and the allele frequency of T of MTHFR gene in experimental group were significant higher than that in control group(P<0.05).There were no significant differences in ERα-Pvu Ⅱ genotypes and ERα-Xba Ⅰgenotypes between experimental group and control group(P>0.05),but the allele frequency of P in experimental group was higher than that in control group(P<0.05).Conclusion:The T allele of MTHFR gene polymorphism and the P allele of ERα-PvuⅡ gene polymorphism may be associated with postpartum depression susceptibility.
引文
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[5]Lewis SJ,Araya R,Leary S,et al.Folic acid supplementation during pregnancy may protect against depression 21months after pregnancy,an effect modified by MTHFR-C677Tgenotype[J].Eur J Clin Nutr,2012,66(1):97-103.
[6]Jones I,Middle F,Mc Candless F,et al.Molecular genetic studies of bipolar disorder and puerperal psychosis at two polymorphisms in the estrogen receptor a gene(ESR1)[J].Am J Med Genet,2000,96(6):850-853.
[2]武勇力,魏镜,李舜伟.5-羟色胺转运蛋白基因多态性与抑郁症发病及抗抑郁治疗关系的研究进展[J].中华精神科杂志,2006,39(4):244-246.
[3]朱跃兰,唐启盛,杨歆科,等.产后抑郁症生物学相关病因及发病机制研究进展[J].北京中医药,2013(3):168-176.
[4]Brown GW,Halris TO.Depression and the serotonin transporter 5-HTTLPR polymorphism:a review and a hypothesis concerning gene-environment interaetion[J].Journal of Affective Disorders,2008,111(1):1-12.
[5]Lewis SJ,Araya R,Leary S,et al.Folic acid supplementation during pregnancy may protect against depression 21months after pregnancy,an effect modified by MTHFR-C677Tgenotype[J].Eur J Clin Nutr,2012,66(1):97-103.
[6]Jones I,Middle F,Mc Candless F,et al.Molecular genetic studies of bipolar disorder and puerperal psychosis at two polymorphisms in the estrogen receptor a gene(ESR1)[J].Am J Med Genet,2000,96(6):850-853.