基于Miseq法研究纳豆芽孢杆菌制剂对小鼠肠道菌群的影响
|
摘要
以正常小鼠和抗生素介导肠道菌群失衡的小鼠为动物试验模型,采用纳豆芽孢杆菌制剂进行体内灌胃试验和Miseq方法研究纳豆芽孢杆菌制剂对小鼠肠道菌群的影响并进行生物信息分析。分析多样本间的肠道菌群的多样性和单样本的门水平及属水平上的分类情况;通过聚类分析方法分析小鼠肠道菌群在不同时间的变化情况,并对纳豆芽孢杆菌制剂进行剂量分析。
Based on the normal mice and low intestinal flora in mice with antibiotics as animal testing model,the regulation of intestinal flora in mice by Bacillus natto preparationwas studied using Bacillus natto preparation in vivo experimental and miseq method.Further the biological information of mice was analyzed.The diversity of intestinal flora among multiple samples and the classification of the single sample's door and its level were analyzed.The situation of the number of bacteria which was changed in Bacillus natto and probiotics and pathogens was analyzed.Ultimately,The situation of the number of bacteria which was changed in the intestinal flora at different time was analyzed.The dose of Bacillus natto preparation was analyzed and determined.
Based on the normal mice and low intestinal flora in mice with antibiotics as animal testing model,the regulation of intestinal flora in mice by Bacillus natto preparationwas studied using Bacillus natto preparation in vivo experimental and miseq method.Further the biological information of mice was analyzed.The diversity of intestinal flora among multiple samples and the classification of the single sample's door and its level were analyzed.The situation of the number of bacteria which was changed in Bacillus natto and probiotics and pathogens was analyzed.Ultimately,The situation of the number of bacteria which was changed in the intestinal flora at different time was analyzed.The dose of Bacillus natto preparation was analyzed and determined.
引文
[1]刘玉军,张晓峰.抗生素滥用的危害及防范[J].中外医疗,2012,973(1):188-189.
[2]胡燕,白继庚,胡先明,等.我国抗生素滥用现状、原因及对策探讨[J].中国社会医学杂志,2013,30(2):128-130.
[3]CARLES UBEDA,ERIC G.Pamer.Antibiotics,microbiota,and immune defense[J].Trends in Immunology,2012,33(9):459-466.
[4]XU X,HUANG Q,MAO Y,et al.Immunomodulatory effects of Bacillus subtilis(natto)B4 spores on murine macrophages[J].Microbiology and Immunology,2012,56(12):817-824.
[5]曹靖文,黄占旺,吴少福,等.纳豆菌糖肽对RAW264.7巨噬细胞的免疫调节作用[J].现代食品科技,2015,31(9):61-67.
[6]OZAWA K,YOKOTA H,KIMURA M,et al.Effects of administration of Bacillus subtilis strain BNon intestinal flora of weanling piglets[J].Nippon Juigaku Zasshi.The Japanese Journal of Veterinary Science,1981,43(5):771-775.
[7]陈兵,朱凤香,陈巧云,等.纳豆芽孢杆菌分离纯化及对大白鼠肠道微生态系统的影响[J].浙江农业学报,2003,15(4):223-227.
[8]黄俊才,林映才,冯定远,等.纳豆菌、甘露寡糖对仔猪肠道pH、微生物区系及肠黏膜形态的影响[J]畜牧兽医学报,2005,36(10):1021-1027.
[9]吴德华,杜莉,徐汉坤,等.纳豆芽孢杆菌对仔犬日增重和肠道菌群的影响[J].家畜生态学报,2009,30(3):64-67.
[10]INOOKA S.Effects of transfer of cell from spleen of adult fowl on antibody-producing ability in chicken.Ⅱ[J].Japanese Journal of Veterinary Science,1971,33(5):166-132.
[11]刘宇,朱战波,车车,等.复合活菌制剂对断奶仔猪生长性能及血清溶菌酶含量的影响[J].中国微生态学杂志,2010,22(9):784-787.
[12]李云锋,邓军,张锦华,等.纳豆芽孢杆菌对仔猪小肠局部天然免疫及TLR表达的影响[J].畜牧兽医学报,2011,42(4):562-566.
[13]耿春银,张敏,孙健,等.纳豆菌微生态制剂替代抗生素用于断奶仔猪饲料的研究[J].中国饲料,2010,33(15):24-27.
[14]李江伟.纳豆菌代谢产物理化性质的研究[J].中国生化药物杂志,1999,20(3):148-150.
[15]郭彤,胥保华,马玉龙,等.嗜酸乳杆菌和两歧双歧菌体外粘附Caco-2细胞及其对病原菌粘附性能的影响[J].中国兽医学报,2008,28(5):527-531.
[16]刘慧.现代食品微生物学实验技术[M].北京:中国轻工业出版社,2011.
[17]高巍,孟庆翔.生长育肥猪胃肠道正常厌氧菌群的数量和分区[J].中国农业大学学报,2000,5(5):88-93.
[18]张帅.2,4-二硝基苯甲醚的零价铁强化生物还原技术研究[D].南京:南京理工大学,2015:24-25.
[19]陈燕.缺氧状态下吡啶的生物强化降解技术研究[D]南京:南京理工大学,2015:55-56.
[20]PALA-OZKOK I,REHMAN A,YAGCI N,et al Characteristics of mixed microbial culture at different sludge ages:Effect on variable kinetics for substrate utilization[J].Bioresource Technology,2012,126(6):274-282.
[21]江美玲.2型糖尿病及其前期状态人群肠道菌群特征分析[D].广州:南方医科大学,2013:19-21.
[22]SCHAREK L,GUTH J,REITER K,et al.Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets[J].Veterinary Immunology and Immunopathology,2005,105(1/2):151-161.
[23]ZHU Y H,LI X Q,ZHANG W,et al.Dose-dependent effects of Lactobacillus rhamnosus on serum interleukin-17 production and intestinal T-cell responses in pigs challenged with Escherichia coli[J].Applied and Environmental Microbiology,2014,80(5):1787-1798.
[24]BERNET M F,BRASSART D,NEESER J R,et al.Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria[J].Gut,1994,35(4):483-489.
[25]FUJITA M,ITO Y,HONG K,et al.Characterization of nattokinase-degraded products from human fibinogen or cross-linked fibrin[J].Fibrinolysis,1995,9(3):157-164.
[26]蔡凯凯,黄占旺,叶德文,等.益生菌调节肠道菌群及免疫调节作用机理[J].中国饲料,2011,33(18):34-37.
[27]张英春,韩雪,单毓娟,等.益生菌抑制致病菌作用的机制研究进展[J].微生物学通报,2012,39(9):1306-1313.
[2]胡燕,白继庚,胡先明,等.我国抗生素滥用现状、原因及对策探讨[J].中国社会医学杂志,2013,30(2):128-130.
[3]CARLES UBEDA,ERIC G.Pamer.Antibiotics,microbiota,and immune defense[J].Trends in Immunology,2012,33(9):459-466.
[4]XU X,HUANG Q,MAO Y,et al.Immunomodulatory effects of Bacillus subtilis(natto)B4 spores on murine macrophages[J].Microbiology and Immunology,2012,56(12):817-824.
[5]曹靖文,黄占旺,吴少福,等.纳豆菌糖肽对RAW264.7巨噬细胞的免疫调节作用[J].现代食品科技,2015,31(9):61-67.
[6]OZAWA K,YOKOTA H,KIMURA M,et al.Effects of administration of Bacillus subtilis strain BNon intestinal flora of weanling piglets[J].Nippon Juigaku Zasshi.The Japanese Journal of Veterinary Science,1981,43(5):771-775.
[7]陈兵,朱凤香,陈巧云,等.纳豆芽孢杆菌分离纯化及对大白鼠肠道微生态系统的影响[J].浙江农业学报,2003,15(4):223-227.
[8]黄俊才,林映才,冯定远,等.纳豆菌、甘露寡糖对仔猪肠道pH、微生物区系及肠黏膜形态的影响[J]畜牧兽医学报,2005,36(10):1021-1027.
[9]吴德华,杜莉,徐汉坤,等.纳豆芽孢杆菌对仔犬日增重和肠道菌群的影响[J].家畜生态学报,2009,30(3):64-67.
[10]INOOKA S.Effects of transfer of cell from spleen of adult fowl on antibody-producing ability in chicken.Ⅱ[J].Japanese Journal of Veterinary Science,1971,33(5):166-132.
[11]刘宇,朱战波,车车,等.复合活菌制剂对断奶仔猪生长性能及血清溶菌酶含量的影响[J].中国微生态学杂志,2010,22(9):784-787.
[12]李云锋,邓军,张锦华,等.纳豆芽孢杆菌对仔猪小肠局部天然免疫及TLR表达的影响[J].畜牧兽医学报,2011,42(4):562-566.
[13]耿春银,张敏,孙健,等.纳豆菌微生态制剂替代抗生素用于断奶仔猪饲料的研究[J].中国饲料,2010,33(15):24-27.
[14]李江伟.纳豆菌代谢产物理化性质的研究[J].中国生化药物杂志,1999,20(3):148-150.
[15]郭彤,胥保华,马玉龙,等.嗜酸乳杆菌和两歧双歧菌体外粘附Caco-2细胞及其对病原菌粘附性能的影响[J].中国兽医学报,2008,28(5):527-531.
[16]刘慧.现代食品微生物学实验技术[M].北京:中国轻工业出版社,2011.
[17]高巍,孟庆翔.生长育肥猪胃肠道正常厌氧菌群的数量和分区[J].中国农业大学学报,2000,5(5):88-93.
[18]张帅.2,4-二硝基苯甲醚的零价铁强化生物还原技术研究[D].南京:南京理工大学,2015:24-25.
[19]陈燕.缺氧状态下吡啶的生物强化降解技术研究[D]南京:南京理工大学,2015:55-56.
[20]PALA-OZKOK I,REHMAN A,YAGCI N,et al Characteristics of mixed microbial culture at different sludge ages:Effect on variable kinetics for substrate utilization[J].Bioresource Technology,2012,126(6):274-282.
[21]江美玲.2型糖尿病及其前期状态人群肠道菌群特征分析[D].广州:南方医科大学,2013:19-21.
[22]SCHAREK L,GUTH J,REITER K,et al.Influence of a probiotic Enterococcus faecium strain on development of the immune system of sows and piglets[J].Veterinary Immunology and Immunopathology,2005,105(1/2):151-161.
[23]ZHU Y H,LI X Q,ZHANG W,et al.Dose-dependent effects of Lactobacillus rhamnosus on serum interleukin-17 production and intestinal T-cell responses in pigs challenged with Escherichia coli[J].Applied and Environmental Microbiology,2014,80(5):1787-1798.
[24]BERNET M F,BRASSART D,NEESER J R,et al.Lactobacillus acidophilus LA 1 binds to cultured human intestinal cell lines and inhibits cell attachment and cell invasion by enterovirulent bacteria[J].Gut,1994,35(4):483-489.
[25]FUJITA M,ITO Y,HONG K,et al.Characterization of nattokinase-degraded products from human fibinogen or cross-linked fibrin[J].Fibrinolysis,1995,9(3):157-164.
[26]蔡凯凯,黄占旺,叶德文,等.益生菌调节肠道菌群及免疫调节作用机理[J].中国饲料,2011,33(18):34-37.
[27]张英春,韩雪,单毓娟,等.益生菌抑制致病菌作用的机制研究进展[J].微生物学通报,2012,39(9):1306-1313.