不同pH值对两种藻的生长及叶绿素荧光的影响
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摘要
以小球藻和铜绿微囊藻为研究对象,应用叶绿素荧光技术研究不同pH值控制下对两种藻的生长及叶绿素荧光特性的影响.研究结果显示:不同pH值对两种藻细胞的光合具有明显抑制作用.小球藻与铜绿微囊藻在pH 7下生长情况没有其他三种酸碱度控制下生长情况好.铜绿微囊藻适宜的pH为8～9,小球藻在pH为8～10下生长情况较好,尤其在pH 10情况下最好.此外两种藻的反应中心比活性参数和能量分配比率在一定程度上也受到一定影响.反应中心比活性的变化与Fv/Fm有很好的一致性.能量分配比率体现出藻的适应性调节机制.藻的最佳生长情况pH与内源氮磷的释放情况相似.
To find out the effects of different pH control on the growth and Chlorophyll fluorescence of two kinds of algae,by use of the Chlorophyll fluorescence technology,chlorella microcystis and microcystis aeruginosa were taken as the research subjects to test the effects of different pH values on the growth and chlorophyll fluorescence characteristics.It was found that the photosynthesis of the two algal cells was significantly inhibited by different pH values.Chlorella and Microcystis aeruginosa did not grow as well at pH 7 as the other three pH levels.The optimum pH of microcystis aeruginosa was at 8～9,and the growth of Microcystis aeruginosa was better at pH 8～10,especially pH 10.In addition,the specific activity parameters and energy distribution ratio of the reaction center of the two algae were also affected to some extent.The change of specific activity of the reaction center was observed to be in good consistency with Fv/Fm.The energy distribution ratio takes on the adaptive regulation mechanism of algae.The optimum growth pH of algae was something similar to the release of endogenous nitrogen and phosphorus.
To find out the effects of different pH control on the growth and Chlorophyll fluorescence of two kinds of algae,by use of the Chlorophyll fluorescence technology,chlorella microcystis and microcystis aeruginosa were taken as the research subjects to test the effects of different pH values on the growth and chlorophyll fluorescence characteristics.It was found that the photosynthesis of the two algal cells was significantly inhibited by different pH values.Chlorella and Microcystis aeruginosa did not grow as well at pH 7 as the other three pH levels.The optimum pH of microcystis aeruginosa was at 8～9,and the growth of Microcystis aeruginosa was better at pH 8～10,especially pH 10.In addition,the specific activity parameters and energy distribution ratio of the reaction center of the two algae were also affected to some extent.The change of specific activity of the reaction center was observed to be in good consistency with Fv/Fm.The energy distribution ratio takes on the adaptive regulation mechanism of algae.The optimum growth pH of algae was something similar to the release of endogenous nitrogen and phosphorus.
引文
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[16]贾浩,郝建博,曹洪波,等.遮荫对‘保佳红’桃树叶片快速叶绿素荧光诱导动力学曲线的影响[J].西北植物学报,2015,35(9):1861-1867.
[17]李鹏民,高辉远.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.
[18]杨燕君,许金铸,吴忠兴,等.无机磷对多甲藻生长及叶绿素荧光的影响[J].西南大学学报(自然科学版),2017,39(8):26-33.
[19]周贤兵,齐泽民,杨凯,等.pH对蒙溪河底泥氮磷释放影响的研究[J].内江师范学院学报,2006,21(S1):232-234.
[20]陶敏,王永明,谢碧文,等.沱江浮游生物群落时空分布及相关环境因子分析[J].水生生物学报,2016,40(2):301-312.
[21]曹六俊,叶丽丽,赵蕾,等.内江市主城区沱江丰水期水质调查研究[J].内江师范学院学报,2008,23(B08):263-266.
[2]毕相东,张树林,刘红玉,等.光限制胁迫协同pH与氮磷比对铜绿微囊藻生长的影响[J].信阳师范学院学报(自然科学版),2014,27(1):38-41.
[3]刘加慧,杨洪帅,王辉.温度,盐度和pH对小球藻生长率的联合效应[J].水生生物学报,2014,38(3):446-453.
[4]陈家长,王菁,裘丽萍,等.pH对鱼腥藻和普通小球藻生长竞争的影响[J].生态环境学报,2014,23(2):289-294.
[5]张青田,王新华,林超,等.水体pH和铜绿微囊藻增殖的关系研究[J].水生态学杂志,2011,32(2):52-56.
[6]KAUTSKY H,HIRSCH A.Neue versuche zur kohlens-aureassimilation[J].Naturwissenschaften,1931,19(48):964-964.
[7]SCHREIBER U.Pulse-amplitude-modulation(PAM)fluorometry and saturation pulse method:an overview[M]//Chlorophyll a fluorescence.Springer,Dordrecht,2004:279-319.
[8]邓闻杨,罗学刚,罗蓝,等.三种微生物对铀胁迫下凤眼莲荧光生理及铀累积特性的影响[J].农业环境科学学报,2018,37(8):1626-1633.
[9]LEIZHEN L,JIANJUN W,HONGKUI Z,et al.Chlorophyll Fluorescence and Its Progress in Detecting Water Stress[J].Spectroscopy and Spectral Analysis,2017,37(9):2780-2787.
[10]VAN HEERDEN P D R,STRASSER R J,KRUGER G H J.Reduction of dark chilling stress in N2-fixing soybean by nitrate as indicated by chlorophyll a fluorescence kinetics[J].Physiologia Plantarum,2004,121(2):239-249.
[11]贺雪,刘果,刘天凤,等.黄河水库水质评价:基于浮游植物群落周年变化[J].内江师范学院学报.2018,33(8):116-123.
[12]郑朔方,杨苏文,金相灿.铜绿微囊藻生长的营养动力学[J].环境科学,2005,26(2):152-156.
[13]魏显珍,王淑智,潘响亮.盐胁迫对喜钙念珠藻生理活性的影响及钙的胁迫缓解效应[J].应用与环境生物学报,2013,19(4):655-662.
[14]薛凌展,陈小晨,黄种持,等.温度和磷交互作用对铜绿微囊藻和小球藻生长的影响[J].安徽农学通报,2011,17(13):23-25.
[15]游波,邓春暖,夏春英,等.铜胁迫对铜绿微囊藻叶绿素荧光的影响[J].安徽农业科学,2015,43(32):29-31.
[16]贾浩,郝建博,曹洪波,等.遮荫对‘保佳红’桃树叶片快速叶绿素荧光诱导动力学曲线的影响[J].西北植物学报,2015,35(9):1861-1867.
[17]李鹏民,高辉远.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报,2005,31(6):559-566.
[18]杨燕君,许金铸,吴忠兴,等.无机磷对多甲藻生长及叶绿素荧光的影响[J].西南大学学报(自然科学版),2017,39(8):26-33.
[19]周贤兵,齐泽民,杨凯,等.pH对蒙溪河底泥氮磷释放影响的研究[J].内江师范学院学报,2006,21(S1):232-234.
[20]陶敏,王永明,谢碧文,等.沱江浮游生物群落时空分布及相关环境因子分析[J].水生生物学报,2016,40(2):301-312.
[21]曹六俊,叶丽丽,赵蕾,等.内江市主城区沱江丰水期水质调查研究[J].内江师范学院学报,2008,23(B08):263-266.
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