毛冬青皂苷B_3的抗血栓作用研究
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摘要
目的从毛冬青根中分离、鉴定毛冬青皂苷B3,研究其抗血栓作用。方法色谱法、重结晶法分离、精制毛冬青皂苷B3,薄层色谱对照及波谱分析法对毛冬青皂苷B3进行结构鉴定。采用体内血栓形成试验、体外血凝块溶解试验及二磷酸腺苷(ADP)诱导血小板聚集试验,观察毛冬青皂苷B3的抗血栓作用。结果体内抗血栓试验显示,毛冬青皂苷B3对胶原蛋白-肾上腺素诱发的血栓形成所致小鼠的偏瘫和死亡及FeCl3诱导的大鼠腹主动脉血栓形成均有明显的抑制作用;在体外实验中,对家兔血凝块无明显的溶解作用,对ADP诱导的离体家兔血小板聚集也无明显的抑制作用。结论毛冬青皂苷B3可能为毛冬青抗血栓作用的物质基础之一,其体内抗血栓形成的作用可能与其促进血凝块溶解和抗ADP诱导的血小板聚集无关。
Objective To separate and prepare ilexsaponin B3(IB3) from the roots of Ilex pubescens,and to study its antithrombotic activity.Methods Chromatography and recrystallization were used in the separation and purification of IB3,and the structure was identified by PTLC and1H-NMR.Antithrombotic activity of IB3was observed by in vivo thrombosis test,solubility test of blood clot in vitro,and platelet aggregation in rabbits induced by adenosine diphosphate(ADP).Results IB3 had significant inhibition on thrombosis-caused hemiplegia and death induced by collagen-epinephrine in mice,and on abdominal arterial thrombotic model induced by FeCl3in rats.IB3 neither had dissolution solubility experiment of blood clot in rabbits nor had inhibition on platelet aggregation in rabbits induced by ADP.Conclusion IB3may be one of the ilexolic material bases for the antithrombotic activity,but it may have no correlation with increasing thrombolysis and platelet aggregation induced by ADP.
Objective To separate and prepare ilexsaponin B3(IB3) from the roots of Ilex pubescens,and to study its antithrombotic activity.Methods Chromatography and recrystallization were used in the separation and purification of IB3,and the structure was identified by PTLC and1H-NMR.Antithrombotic activity of IB3was observed by in vivo thrombosis test,solubility test of blood clot in vitro,and platelet aggregation in rabbits induced by adenosine diphosphate(ADP).Results IB3 had significant inhibition on thrombosis-caused hemiplegia and death induced by collagen-epinephrine in mice,and on abdominal arterial thrombotic model induced by FeCl3in rats.IB3 neither had dissolution solubility experiment of blood clot in rabbits nor had inhibition on platelet aggregation in rabbits induced by ADP.Conclusion IB3may be one of the ilexolic material bases for the antithrombotic activity,but it may have no correlation with increasing thrombolysis and platelet aggregation induced by ADP.
引文
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[4]冯峰,朱明晓,谢宁.毛冬青化学成分研究[J].中国药学杂志,2008,43(10):732-736.
[5]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1994.
[6]王四旺,施新猷,黄传贵,等.中药药效学研究与评价[M].西安:陕西科学技术出版社,2005.
[7]李仪奎.中药药理实验方法学[M].上海:上海科学技术出版社,2006.
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