蒲地蓝消炎片定性定量研究
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摘要
目的对蒲地蓝消炎片进行定性定量方法研究。方法对处方中的黄芩、蒲公英、苦地丁、板蓝根4味药材采用显微或薄层色谱(TLC)进行鉴别,采用高效液相色谱法测定黄芩中有效成分黄芩苷的含量,C_(18)色谱柱,流动相:甲醇-水-磷酸(47∶53∶0.2)为流动相,检测波长280 nm,流速为1.0 mL·min~(-1)。结果在薄层色谱中可检出黄芩、蒲公英、苦地丁、板蓝根,含量测定中黄芩苷进样量在5.008～100.16μg范围内线性关系良好(r=0.999 8);黄芩苷的加样回收率为98.87%,RSD为0.95%(n=9)。结论本方法操作简单,受外界干扰因素少,重现性好,可用于蒲地蓝消炎片的质量控制。
Objective To establish a method for the quality control of Pudilan Xiaoyan Tablets.Methods Microscope,TLC were used to identify the contents of Scutellaria baicalensis Georgi,dandelion,Corydalis bungeana,Radix Isatidis;HPLC was used to measure the effective content of baicalin in Scutellaria baicalensis Georgi,C_(18) column,the mobile phase was methanol-water-phosphoric acid(47∶53∶0.2),the wavelength was 280 nm,and the flow rate was 1.0 mL·min~(-1).Results The contents of Scutellaria baicalensis Georgi,dandelion,Corydalis bungeana,Radix Isatidis can be detected by TLC,baicalin showed a good linear in the range of 5.00 8～100.16 μg(r=0.999 8),the average recovery of baicalin was 98.87%,RSD=0.95%(n=9).Conclusion The method was sample,reliable,and can be used for the quality control of Pudilan Xiaoyan Tablets.
Objective To establish a method for the quality control of Pudilan Xiaoyan Tablets.Methods Microscope,TLC were used to identify the contents of Scutellaria baicalensis Georgi,dandelion,Corydalis bungeana,Radix Isatidis;HPLC was used to measure the effective content of baicalin in Scutellaria baicalensis Georgi,C_(18) column,the mobile phase was methanol-water-phosphoric acid(47∶53∶0.2),the wavelength was 280 nm,and the flow rate was 1.0 mL·min~(-1).Results The contents of Scutellaria baicalensis Georgi,dandelion,Corydalis bungeana,Radix Isatidis can be detected by TLC,baicalin showed a good linear in the range of 5.00 8～100.16 μg(r=0.999 8),the average recovery of baicalin was 98.87%,RSD=0.95%(n=9).Conclusion The method was sample,reliable,and can be used for the quality control of Pudilan Xiaoyan Tablets.
引文
[1] 孙光,孟繁姝,孙凯云,等.蒲地蓝消炎片的研究进展[J].健康天地,2010,4(1):92.
[2] 蒋磊,孟祥松,李军,等.蒲地蓝消炎口服液质量控制方法的研究[J].齐鲁药事,2012,31(2):75-77.
[3] 宋茹,迟归兵,袁继民.蒲地蓝消炎片的质量标准研究[J].解放军药学学报,2008,24(3):229-230,258.
[4] 李凯,逯海龙.蒲地蓝消炎片质量标准的研究[J].宁夏医科大学学报,2012,34(8):859-860.
[5] 曹兵,胡春雪,熊叶红,等.蒲地蓝消炎片质量控制方法研究[J].大理学院学报,2008,7(8):4-6.
[6] 国家药典委员会.中华人民共和国药典2015年版(一部)[S].北京:中国医药科技出版社,2015.
[7] 李振宇,胡景莲,康廷田,等.高效液相色谱法测定连翘败毒丸中黄芩苷的含量[J].辽宁中医杂志,2013,40(4):756-757.
[8] 叶曼红,石晓.HPLC测定牛黄丸中黄芩苷的含量[J].食品与药品,2007,9(9A):27-29.
[2] 蒋磊,孟祥松,李军,等.蒲地蓝消炎口服液质量控制方法的研究[J].齐鲁药事,2012,31(2):75-77.
[3] 宋茹,迟归兵,袁继民.蒲地蓝消炎片的质量标准研究[J].解放军药学学报,2008,24(3):229-230,258.
[4] 李凯,逯海龙.蒲地蓝消炎片质量标准的研究[J].宁夏医科大学学报,2012,34(8):859-860.
[5] 曹兵,胡春雪,熊叶红,等.蒲地蓝消炎片质量控制方法研究[J].大理学院学报,2008,7(8):4-6.
[6] 国家药典委员会.中华人民共和国药典2015年版(一部)[S].北京:中国医药科技出版社,2015.
[7] 李振宇,胡景莲,康廷田,等.高效液相色谱法测定连翘败毒丸中黄芩苷的含量[J].辽宁中医杂志,2013,40(4):756-757.
[8] 叶曼红,石晓.HPLC测定牛黄丸中黄芩苷的含量[J].食品与药品,2007,9(9A):27-29.