大豆蛋白与大豆低聚肽对负氮平衡老年小鼠表皮创伤感染下的免疫调节作用
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摘要
目的:探讨大豆蛋白与大豆低聚肽对负氮平衡老年小鼠在金黄色葡萄球菌表皮创伤感染下的免疫调节及炎症抑制的影响。方法:实验设空白组(正氮平衡)、模型组(负氮平衡)、蛋白组(负氮平衡灌胃大豆蛋白)、低聚肽组(负氮平衡灌胃大豆低聚肽),其中,蛋白组与低聚肽组灌胃液体总含氮量相等,以体质量计为650 mg/kg。经过背部表皮创伤感染金黄色葡萄球菌后灌胃,观察大豆蛋白与大豆低聚肽对小鼠体液免疫水平、炎症抑制水平、股直肌质量等的影响。结果:低蛋白饲料喂养能显著降低小鼠体质量、氮摄入量及血清总蛋白质量浓度(P<0.05),大豆蛋白与大豆低聚肽能显著提高小鼠血清免疫球蛋白(immunoglobulin,Ig)M、Ig G及Ig A质量浓度(P<0.05),显著降低巨噬细胞炎性、RANTES质量浓度(P<0.05),可降低炎症因子白细胞介素(interleukin,IL)-1β、IL-6和TNF-α质量浓度,差异不显著,能显著提高股直肌湿质量与体质量比(P<0.05),大豆低聚肽与大豆蛋白相比,可显著提高Ig G质量浓度,显著降低蛋白-2质量浓度。结论:大豆蛋白与大豆低聚肽对体液免疫功能有显著的提高作用,对炎症反应有一定抑制作用,对肌肉萎缩有一定恢复作用,大豆低聚肽较大豆蛋白对体液免疫功能的提高以及早期炎症抑制作用更加显著。
Objective: To investigate immune modulation and inflammatory inhibition of soybean protein and soybean oligopeptides on Staphylococcus aureus-induced epidermal trauma in aged mice with negative nitrogen balance. Methods: Mice were divided into four groups: blank control group(positive nitrogen balance), model group(negative nitrogen balance), soybean protein group(administration with soybean protein plus negative nitrogen balance), soybean oligopeptides group(administration with soybean oligopeptides plus negative nitrogen balance), among them, the total nitrogen content of the liquid in the soybean protein group and the soybean oligopeptides group was equal, calculated as body mass of 650 mg/kg. The mice were orally administered after back epidermal wound infection with Staphylococcus aureus. The effects of soybean protein and soybean oligopeptides on humoral immunity level, inflammation inhibition and rectus muscle mass were investigated. Results: Low protein diet significantly reduced body mass, nitrogen intake and total serum protein in mice(P < 0.05). Soybean protein and soybean oligopeptides significantly increased the serum levels of immunoglobulin(Ig) M, Ig G and Ig A(P < 0.05), and significantly reduced the contents of macrophage inflammatory protein-2(MIP-2) and RANTES(P < 0.05), decreased the contents of interleukin(IL)-1β, IL-6 and TNF-α but without any significant difference, and significantly augmented the ratio of rectus femoris to body mass(P < 0.05). Compared with soybean protein, soybean oligopeptides significantly increased serum Ig G level, and significantly reduced MIP-2 level(P < 0.05). Conclusion: Soybean protein and soybean oligopeptides can significantly enhance humoral immune function, inhibit inflammatory response and restore muscle atrophy. Soybean oligopeptides can enhance humoral immune function and inhibit early inflammatory response more significantly than soy protein.
Objective: To investigate immune modulation and inflammatory inhibition of soybean protein and soybean oligopeptides on Staphylococcus aureus-induced epidermal trauma in aged mice with negative nitrogen balance. Methods: Mice were divided into four groups: blank control group(positive nitrogen balance), model group(negative nitrogen balance), soybean protein group(administration with soybean protein plus negative nitrogen balance), soybean oligopeptides group(administration with soybean oligopeptides plus negative nitrogen balance), among them, the total nitrogen content of the liquid in the soybean protein group and the soybean oligopeptides group was equal, calculated as body mass of 650 mg/kg. The mice were orally administered after back epidermal wound infection with Staphylococcus aureus. The effects of soybean protein and soybean oligopeptides on humoral immunity level, inflammation inhibition and rectus muscle mass were investigated. Results: Low protein diet significantly reduced body mass, nitrogen intake and total serum protein in mice(P < 0.05). Soybean protein and soybean oligopeptides significantly increased the serum levels of immunoglobulin(Ig) M, Ig G and Ig A(P < 0.05), and significantly reduced the contents of macrophage inflammatory protein-2(MIP-2) and RANTES(P < 0.05), decreased the contents of interleukin(IL)-1β, IL-6 and TNF-α but without any significant difference, and significantly augmented the ratio of rectus femoris to body mass(P < 0.05). Compared with soybean protein, soybean oligopeptides significantly increased serum Ig G level, and significantly reduced MIP-2 level(P < 0.05). Conclusion: Soybean protein and soybean oligopeptides can significantly enhance humoral immune function, inhibit inflammatory response and restore muscle atrophy. Soybean oligopeptides can enhance humoral immune function and inhibit early inflammatory response more significantly than soy protein.
引文
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[4]程天德,张韬,梁延省.大豆低聚肽降胆固醇作用研究[J].食品工业科技,2012,33(22):375-377.DOI:10.13386/j.issn1002-0306.2012.22.087.
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[7]张连慧,贺寅,刘新旗.大豆肽的研究进展及其发展前景[J].食品工业科技,2012,33(22):406-408;413.DOI:10.13386/j.issn1002-0306.2012.22.093.
[8]王鑫,徐丽萍.大豆低聚肽抗氧化及抗疲劳作用评价[J].食品工业科技,2013,34(24):359-362;375.DOI:10.13386/j.issn1002-0306.2013.24.023.
[9]DOMíNGUEZ-VEGA E,KOTKOWSKA O,CONCEPCIóN GM,et al.Fast determination of the functional peptide soymetide in different soybean derived foods by capillary-high performance liquid chromatography[J].Journal of Chromatography A,2011,1218(30):4928-4933.DOI:10.1016/j.chroma.2011.05.055.
[10]MESSINA M.A brief historical overview of the past two decades of soy and isoflavone research[J].The Journal of Nutrition,2010,140(7):1350S-1354S.DOI:10.3945/jn.109.118315.
[11]吴本俨.老年人消化系统的衰老改变[J].中华老年医学杂志,2007,26(1):76-78.DOI:10.3760/j:issn:0254-9026.2007.01.025.
[12]张伟,郑松柏,于晓峰,等.老年人胃酸和胃蛋白酶研究进展[J].中国老年学杂志,2010,30(18):2717-2719.DOI:10.3969/j.issn.1005-9202.2010.18.078.
[13]PAE M,WU D Y.Nutritional modulation of age-related changes in the immune system and risk of infection[J].Nutrition Research,2017,41:14-35.DOI:10.1016/j.nutres.2017.02.001.
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[15]李帅军,刘欣.胃肠道恶性肿瘤围术期肠内免疫营养对术后免疫功能的影响[J].实用临床医药杂志,2012,16(17):46-48.
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[18]FROMMELT L,BIELOHUBY M,MENHOFER D,et al.Effects of low carbohydrate diets on energy and nitrogen balance and body composition in rats depend on dietary protein-to-energy ratio[J].Nutrition,2014,30(7/8):863-868.DOI:10.1016/j.nut.2013.11.009.
[19]赵瑞利,韩文瑜,韩俊友,等.新型牛蛙抗菌肽Temporin-La对金黄色葡萄球菌感染模型小鼠的治疗效果[J].中国生物制品学杂志,2013,26(8):1143-1146;1150.DOI:10.13200/j.cjb.2013.08.100.zhaorl.014.
[20]王丹虹,齐华雪,罗开菊,等.葛根素皮下注射对妊娠期低蛋白饮食孕鼠所产仔鼠体质量及骨质代谢的影响[J].山东医药,2017,57(36):46-48.DOI:10.3969/j.issn.1002-266X.2017.36.014.
[21]侯聪玲.高碘低蛋白对大鼠生长代谢、细胞凋亡的影响及甲状腺的病理形态学变化的研究[J].中国地方病防治杂志,2016,31(6):648-649;710.
[22]潘兴昌,印虹,谷瑞增,等.小麦肽对大鼠氮代谢以及胃肠黏膜结构和功能的影响[J].食品科学,2013,34(5):264-269.DOI:10.7506/spkx1002-6630-201305055.
[23]陈敏,孙建琴,孙建设,等.老年肾衰病人透析治疗氮平衡和营养状况的研究[J].老年医学与保健,2008,14(2):103-106.DOI:10.3969/j.issn.1008-8296.2008.02.010.
[24]喻礼怀,张军,魏宗友,等.饲粮色氨酸水平对扬州鹅蛋白质代谢的影响[J].动物营养学报,2014,26(1):81-89.DOI:10.3969/j.issn.1006-267x.2014.01.012.
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