不同采种地红豆树种子性状多样性分析
|
摘要
【目的】分析不同采种地红豆树种子性状变异及其与地理因子的相关性,为红豆树采种地试验和遗传资源保护、优质种质资源的挖掘提供数据支持。【方法】对采自福建、浙江、安徽、湖南、四川等5省13个采种地的红豆树种子进行纵径、横径、单粒质量、厚度、纵横径比、体积指数等6个性状测量,并采用单因素分析、相关性分析、聚类分析及主成分分析等方法进行统计分析。【结果】红豆树种子性状都具有丰富的变异性(变异系数均大于10%),其中体积指数变异系数最大,为25.39%。利用相关性分析进一步研究发现,纵径、横径、单粒质量、厚度、纵横径比、体积指数等6个性状间均存在显著的相关性,且这些性状与经纬度间也存在显著的正相关或负相关。聚类分析显示13个采种地种子可聚为5个类群,且各类群在6个性状上均有显著性差异。【结论】利用主成分分析及构建综合评价模型发现,13个采种地种子中综合排名前3位的依次为四川雅安、福建沙县和安徽歙县富堨的红豆树种子。
【Objective】 This study analyzed variation on the seed traits of Ormosia hosiei Hemsl. Et Wils. from different producing regions and their correlation with geographical factors to provide data to support seed experiments, the protection of genetic resources, and resource mining of quality germplasm. 【Method】 We collected seeds from 13 O. hosiei origin locations in five provinces including Fujian, Zhejiang, Hunan, Sichuan, and Anhui, to determine differences in characteristics such as longitudinal diameter, transverse diameter, single grain weight, and thickness. Statistics were used to reveal phenotypic variation in seeds from different producing regions and their correlation with geographical factors via single factor analysis, cluster analysis, correlation analysis, and principal component analysis. 【Result】 The phenotypic traits of O. hosiei seeds present abundant variation in characteristics(variability coefficients exceeded 10%). The volume index of variation was the largest, at about 25.39%. Furthermore, correlation analysis revealed that there were significant correlations among the six phenotypic traits, including longitudinal diameter, transverse diameter, single grain weight, thickness ratio of vertical to transverse diameter, and bulk index. Furthermore, these traits were significantly positively or negatively correlated with latitude or longitude. Cluster analysis showed that the seeds of 13 producing regions could be clustered into five groups, and each group presented significant differences in six phenotypic traits. 【Conclusion】 Based on the principal component analysis and construction of a comprehensive evaluation model, these results indicated that the top three seeds are from Sichuan Yaan, Fujian Shaxian, and Anhui Shexian Fuhe. These results provide a basis for the development and utilization of the seed resources of O. hosiei Hemsl. Et Wils.
【Objective】 This study analyzed variation on the seed traits of Ormosia hosiei Hemsl. Et Wils. from different producing regions and their correlation with geographical factors to provide data to support seed experiments, the protection of genetic resources, and resource mining of quality germplasm. 【Method】 We collected seeds from 13 O. hosiei origin locations in five provinces including Fujian, Zhejiang, Hunan, Sichuan, and Anhui, to determine differences in characteristics such as longitudinal diameter, transverse diameter, single grain weight, and thickness. Statistics were used to reveal phenotypic variation in seeds from different producing regions and their correlation with geographical factors via single factor analysis, cluster analysis, correlation analysis, and principal component analysis. 【Result】 The phenotypic traits of O. hosiei seeds present abundant variation in characteristics(variability coefficients exceeded 10%). The volume index of variation was the largest, at about 25.39%. Furthermore, correlation analysis revealed that there were significant correlations among the six phenotypic traits, including longitudinal diameter, transverse diameter, single grain weight, thickness ratio of vertical to transverse diameter, and bulk index. Furthermore, these traits were significantly positively or negatively correlated with latitude or longitude. Cluster analysis showed that the seeds of 13 producing regions could be clustered into five groups, and each group presented significant differences in six phenotypic traits. 【Conclusion】 Based on the principal component analysis and construction of a comprehensive evaluation model, these results indicated that the top three seeds are from Sichuan Yaan, Fujian Shaxian, and Anhui Shexian Fuhe. These results provide a basis for the development and utilization of the seed resources of O. hosiei Hemsl. Et Wils.
引文
[1] 李斌,郑勇奇,林富荣,等.中国林木遗传资源利用与可持续经营状况[J].植物遗传资源学报,2014,15(6):1390-1394.DOI:10.13430/j.cnki.jpgr.2014.06.033.LI B,ZHENG Y Q,LIN F R,et al.The state of utilization and sustainable management of forest genetic resources of China[J].Journal of Plant Genetic Resources,2014,15(6):1390-1394..
[2] BOOY G,HENDRIKS R J,SMULDERS M M,et al.Genetic diversity and the survival of populations[J].Plant Biology,2000,2(4):379-395.DOI:10.1002/zoo.10091.
[3] MASSIMO P,MURREN C J,SCHLICHTING C D.Phenotypic plasticity and evolution by genetic assimilation[J].Journal of Experimental Biology,2006,209(12):2362-2367.DOI:10.1242/jeb.02070.
[4] SCARANO D,RUBIO F,RUIZ J J,et al.Morphological and genetic diversity among and within common bean (Phaseolus vulgaris L.) landraces from the Campania region (Southern Italy)[J].Scientia Horticulturae,2014,180(1):72-78.DOI:10.1016/j.scienta.2014.10.013.
[5] 任华东,姚小华.樟树种子性状产地表型变异研究[J].江西农业大学学报,2000(3):370-375.DOI:10.3969/j.issn.1000-2286.2000.03.013.REN H D,YAO X H.A study on seeds phenotypic variation in natural range of Cinnamomum camphora (L.) Presl[J].Acta Agriculturae Universitatis Jiangxiensis,2000(3):370-375.
[6] 刘鹏,阙生全,刘丽婷,等.红豆树研究现状及濒危保护建议[J].亚热带植物科学,2017,46(1):96-100.DOI:10.3969/j.issn.1009-7791.2017.01.019.LIU P,QUE S Q,LIU L T,et al.Research status and endangered conservation strategy of Ormosia hosiei[J].Subtropical Plant Science,2017,46(1):96-100.
[7] 周志春,楚秀丽.以精细化培育促进红豆树产业发展[J].浙江林业,2018(1):18-19.ZHOU Z C,CHU X L.Refine cultivation to promote the development of Ormosia hosiei industry [J].Zhejiang Forestry,2018(1):18-19.
[8] 于永福.中国野生植物保护工作的里程碑——《国家重点保护野生植物名录(第一批)》出台[J].植物杂志,1999(5):3.YU Y F.A milestone in the conservation of wild plants in China—List of key wild plants under state protection (the first batch) [J].Plant Journal,1999(5):3.
[9] 彭彪,陈孝丑,林雄平,等.红豆树多用途林的营造技术研究[J].宁德师范学院学报(自然科学版),2016(3):247-250.DOI:10.3969/j.issn.2095-2481.2016.03.005.PENG B,CHEN X C,LIN X P,et al.Establishment techniques of all-purpose tree of Ormosia hosiei[J].Journal of Ningde Normal University (Natural Science Edition),2016(3):247-250.
[10] 文珊娜,姜清彬,仲崇禄,等.灰木莲不同种源种子形态变异分析[J].中南林业科技大学学报,2016,36(7):7-11.DOI:10.14067/j.cnki.1673-923x.2016.07.002.WEN S N,JIANG Q B,ZHONG C L,et al.Seed morphology variation analysis of different provenances in Manglietia conifera[J].Journal of Central South University of Forestry & Technology,2016,36(7):7-11.
[11] 潘存祥,许勇,纪海波,等.西瓜种质资源表型多样性及聚类分析[J].植物遗传资源学报,2015,16(1):59-63.DOI:10.13430/j.cnki.jpgr.2015.01.009.PAN C X,XU Y,JI H B,et al.Phenotypic diversity and clustering analysis of watermelon germplasm [J].Journal of Plant Genetic Resources,2015,16(1):59-63.
[12] 欧建德.基于多元统计分析的南方红豆杉幼林修枝技术[J].南京林业大学学报(自然科学版),2016,40(3):183-187.DOI:10.3969/j.issn.1000-2006.2016.03.030.OU J D.Pruning techniques of Taxus chinensis var.mairei young plantations based on multivariate statistical analysis [J].Journal of Nanjing Forestry University (Natural Sciences Edition),2016,40(3):183-187.
[13] 李峰卿,陈焕伟,周志春,等.红豆树优树种子和幼苗性状的变异分析及优良家系的初选[J].植物资源与环境学报,2018,27(2):57-65.DOI:10.3969/j.issn.1674-7895.2018.02.08.LI F Q,CHEN H W,ZHOU Z C,et al.Analysis on variation of seed and seedling traits of superior trees of Ormosia hosiei and primary screening of optimum family[J].Journal of Plant Resources and Environment,2018,27(2):57-65.
[14] 李峰卿,周志春,楚秀丽,等.红豆树优树子代遗传多样性及与生长相关性分析[J].林业科学研究,2018,31(1):42-50.DOI:10.13275/j.cnki.lykxyj.2018.01.005.LI F Q,ZHOU Z C,CHU X L,et al.Analysis of genetic diversity among progenies from plus trees of Ormosia hosiei and its correlation with growth [J].Forest Research,2018,31(1):42-50.
[15] 张兰,张怡,段如雁,等.不同采种地闽楠苗期性状变异及初步选择[J].南京林业大学学报(自然科学版),2016,40(6):33-40.DOI:10.3969/j.issn.1000-2006.2016.06.006.ZHANG L,ZHANG Y,DUAN R Y,et al.Genetic variation among origin locations of Phoebe bournei (Hemsl.) Yang and a preliminary selection based on seedling growth and root traits[J].Journal of Nanjing Forestry University (Natural Sciences Edition),2016,40(6):33-40.
[2] BOOY G,HENDRIKS R J,SMULDERS M M,et al.Genetic diversity and the survival of populations[J].Plant Biology,2000,2(4):379-395.DOI:10.1002/zoo.10091.
[3] MASSIMO P,MURREN C J,SCHLICHTING C D.Phenotypic plasticity and evolution by genetic assimilation[J].Journal of Experimental Biology,2006,209(12):2362-2367.DOI:10.1242/jeb.02070.
[4] SCARANO D,RUBIO F,RUIZ J J,et al.Morphological and genetic diversity among and within common bean (Phaseolus vulgaris L.) landraces from the Campania region (Southern Italy)[J].Scientia Horticulturae,2014,180(1):72-78.DOI:10.1016/j.scienta.2014.10.013.
[5] 任华东,姚小华.樟树种子性状产地表型变异研究[J].江西农业大学学报,2000(3):370-375.DOI:10.3969/j.issn.1000-2286.2000.03.013.REN H D,YAO X H.A study on seeds phenotypic variation in natural range of Cinnamomum camphora (L.) Presl[J].Acta Agriculturae Universitatis Jiangxiensis,2000(3):370-375.
[6] 刘鹏,阙生全,刘丽婷,等.红豆树研究现状及濒危保护建议[J].亚热带植物科学,2017,46(1):96-100.DOI:10.3969/j.issn.1009-7791.2017.01.019.LIU P,QUE S Q,LIU L T,et al.Research status and endangered conservation strategy of Ormosia hosiei[J].Subtropical Plant Science,2017,46(1):96-100.
[7] 周志春,楚秀丽.以精细化培育促进红豆树产业发展[J].浙江林业,2018(1):18-19.ZHOU Z C,CHU X L.Refine cultivation to promote the development of Ormosia hosiei industry [J].Zhejiang Forestry,2018(1):18-19.
[8] 于永福.中国野生植物保护工作的里程碑——《国家重点保护野生植物名录(第一批)》出台[J].植物杂志,1999(5):3.YU Y F.A milestone in the conservation of wild plants in China—List of key wild plants under state protection (the first batch) [J].Plant Journal,1999(5):3.
[9] 彭彪,陈孝丑,林雄平,等.红豆树多用途林的营造技术研究[J].宁德师范学院学报(自然科学版),2016(3):247-250.DOI:10.3969/j.issn.2095-2481.2016.03.005.PENG B,CHEN X C,LIN X P,et al.Establishment techniques of all-purpose tree of Ormosia hosiei[J].Journal of Ningde Normal University (Natural Science Edition),2016(3):247-250.
[10] 文珊娜,姜清彬,仲崇禄,等.灰木莲不同种源种子形态变异分析[J].中南林业科技大学学报,2016,36(7):7-11.DOI:10.14067/j.cnki.1673-923x.2016.07.002.WEN S N,JIANG Q B,ZHONG C L,et al.Seed morphology variation analysis of different provenances in Manglietia conifera[J].Journal of Central South University of Forestry & Technology,2016,36(7):7-11.
[11] 潘存祥,许勇,纪海波,等.西瓜种质资源表型多样性及聚类分析[J].植物遗传资源学报,2015,16(1):59-63.DOI:10.13430/j.cnki.jpgr.2015.01.009.PAN C X,XU Y,JI H B,et al.Phenotypic diversity and clustering analysis of watermelon germplasm [J].Journal of Plant Genetic Resources,2015,16(1):59-63.
[12] 欧建德.基于多元统计分析的南方红豆杉幼林修枝技术[J].南京林业大学学报(自然科学版),2016,40(3):183-187.DOI:10.3969/j.issn.1000-2006.2016.03.030.OU J D.Pruning techniques of Taxus chinensis var.mairei young plantations based on multivariate statistical analysis [J].Journal of Nanjing Forestry University (Natural Sciences Edition),2016,40(3):183-187.
[13] 李峰卿,陈焕伟,周志春,等.红豆树优树种子和幼苗性状的变异分析及优良家系的初选[J].植物资源与环境学报,2018,27(2):57-65.DOI:10.3969/j.issn.1674-7895.2018.02.08.LI F Q,CHEN H W,ZHOU Z C,et al.Analysis on variation of seed and seedling traits of superior trees of Ormosia hosiei and primary screening of optimum family[J].Journal of Plant Resources and Environment,2018,27(2):57-65.
[14] 李峰卿,周志春,楚秀丽,等.红豆树优树子代遗传多样性及与生长相关性分析[J].林业科学研究,2018,31(1):42-50.DOI:10.13275/j.cnki.lykxyj.2018.01.005.LI F Q,ZHOU Z C,CHU X L,et al.Analysis of genetic diversity among progenies from plus trees of Ormosia hosiei and its correlation with growth [J].Forest Research,2018,31(1):42-50.
[15] 张兰,张怡,段如雁,等.不同采种地闽楠苗期性状变异及初步选择[J].南京林业大学学报(自然科学版),2016,40(6):33-40.DOI:10.3969/j.issn.1000-2006.2016.06.006.ZHANG L,ZHANG Y,DUAN R Y,et al.Genetic variation among origin locations of Phoebe bournei (Hemsl.) Yang and a preliminary selection based on seedling growth and root traits[J].Journal of Nanjing Forestry University (Natural Sciences Edition),2016,40(6):33-40.