甜菜高糖抗病品系DP24的选育与利用
|
摘要
DP24是以抗丛根病种质W12为母本,以3个自育的优良品系(KH、1303/111、F111)为父本,自然杂交,其反交组合经过3个世代母根单株选择混合采种,之后进行一次单株自交选择、扩繁,在感病地和非感病地试验鉴定同时进行了3个世代大面积培育母根进行高强度选择而成的高糖抗病甜菜品系。经感病地和非感病地试验鉴定,4年感病地鉴定表现较强的抗病性,根产量比抗病性较强的对照品种增产-4.3%～53.5%,含糖率提高0.82～2.48度,产糖量提高2.9%～64.3%,抗褐斑病和根腐病、较耐丛根病,褐斑病病情比表现抗病的对照品种低0～0.76级,根腐病罹病率低0.5%～10.2%,,丛根病病情指数与耐丛根病品种持平,比甜研8号低9.2。非感病地鉴定根产量比丰产性较强的ZM202减产17.4%,含糖率提高1.93度,产糖量为ZM202的92.6%。DP24作为亲本之一育成甜菜新品种甜研208,作为授粉者与新型细胞质单胚雄性不育系配制杂交组合,优良组合率50%以上。
DP24, the sugar beet lines with high sugar content and disease resistance, was a natural cross using the resistant germplasm W12 as female parent and three fine self-bred lines(KH, 1303/111, F111) as male parent. Its reciprocal cross was selected by 3 generations of mother root to select mixed seed collection. After that, a single plant was selected for self-breeding and propagation was expanded. High intensity selection of maternal roots was carried out in 3 generations in both susceptible and non-susceptible fields at the same time.Four years of susceptible field identification showed strong resistance to disease, root yield increased from-4.3% to 53.5%, the sugar content increased by 0.82%-2.48%, the sugar yield increased by 2.9%-64.3% in comparison with the control. The line was resistant to leaf spot and root rot, and was more tolerance to rhizomania. The leaf spot was 0-0.76 grade lower, and the rate of root rot disease was 0.5%-10.2% lower than the resistant cultivar;and the disease index of rhizomania was the same as that of variety with tolerance to rhizomania, which was 9.2 lower than Tianyan8. In non-susceptible fields identification, the root yield was 17.4% lower than the high yield cultivar ZM202, but the sugar content increased by 1.93%, and sugar yield was 92.6% of ZM202. DP24 was used as one of the parents to breed a new sugar beet variety Tianyan 208. The rate of superior cross combination was more than 50% when DP24 was pollinator with new cytoplasmic monogerm male sterile line.
DP24, the sugar beet lines with high sugar content and disease resistance, was a natural cross using the resistant germplasm W12 as female parent and three fine self-bred lines(KH, 1303/111, F111) as male parent. Its reciprocal cross was selected by 3 generations of mother root to select mixed seed collection. After that, a single plant was selected for self-breeding and propagation was expanded. High intensity selection of maternal roots was carried out in 3 generations in both susceptible and non-susceptible fields at the same time.Four years of susceptible field identification showed strong resistance to disease, root yield increased from-4.3% to 53.5%, the sugar content increased by 0.82%-2.48%, the sugar yield increased by 2.9%-64.3% in comparison with the control. The line was resistant to leaf spot and root rot, and was more tolerance to rhizomania. The leaf spot was 0-0.76 grade lower, and the rate of root rot disease was 0.5%-10.2% lower than the resistant cultivar;and the disease index of rhizomania was the same as that of variety with tolerance to rhizomania, which was 9.2 lower than Tianyan8. In non-susceptible fields identification, the root yield was 17.4% lower than the high yield cultivar ZM202, but the sugar content increased by 1.93%, and sugar yield was 92.6% of ZM202. DP24 was used as one of the parents to breed a new sugar beet variety Tianyan 208. The rate of superior cross combination was more than 50% when DP24 was pollinator with new cytoplasmic monogerm male sterile line.
引文
[1]刘升廷,王燕飞,高卫时,等.对我国甜菜种业发展的思考[J].中国糖料,2017,39(2):71-74.
[2]吴则东,王荣华,张文彬.新时期甜菜糖业面临的机遇、挑战及发展策略[J].中国糖料,2017,39(4):71-73.
[3]李彦丽,马亚怀,柏章才.高配合力甜菜二倍体授粉系Zj401的选育[J].中国糖料,2018,40(6):4-7.
[4]刘升廷,张立明,蔡惠珍,等.甜菜育种动向与展望[J].中国糖料,2016,38(4):56-61.
[5]马龙彪,吴则东,王茂芊,等.甜菜育种的研究进展及未来发展展望[J].中国糖料,2018,40(6):62-65.
[6]张跃彬.我国蔗糖产业安全问题与发展对策[J].中国糖料,2019,41(1):66-68.
[7]陈丽,赵春雷,陈连江,等.甜菜新品种“甜研208”的选育,中国糖料,2013(4):7-8,14.
[8]孙以楚,杨炎生,王华忠,等.甜菜多倍体新品种甜研309的选育,中国糖料,1997(3):1-4.
[9]刘景泉,陈丽,程大友.甜菜多系杂交种甜研八号的选育,中国糖料,1999⑴:1-5.
[10]刘景泉,陈丽,程大友.耐丛根病甜菜杂交种甜研203的选育,中国糖料,1999⑶:9-11.
[11]李彦丽,柏章才,马亚怀.丰产优质抗病甜菜新品种ZM202的选育,中国糖料,2010(3):6-8.
[12]刘景泉,陈丽,程大友.甜菜有粉系轮回选择初报-早代配合力选择,中国甜菜,1993(2):18-24.
[13]陈丽,刘景泉,程大友,等.优质抗病甜菜新品种中甜204的选育,中国糖料,2001(2):1-3.
[14]刘景泉,陈丽.甜菜亲本主要数量性状遗传规律的初步研究,中国甜菜,1984(2):8-19.
[15]Ksenija Taski-Ajdukovic,N Nagl,Zivko Curcic,et al.Estimation of genetic diversity and relationship in sugar beet pollinators based on SSR markers[J].Electronic Journal of Biotechnology,2017,27:1-7.
[16]Monteiro F,Frese L,Castro S,et al.Genetic and Genomic Tools to Asssist Sugar Beet Improvement:The Value of the Crop Wild Relatives[J].Frontiers in Plant Science,2018,9(9):1-8.
[17]Hoffmann C M,Kenter C.Yield Potential of Sugar Beet-Have We Hit the Ceiling?[J].Frontiers in Plant Science,2018,9:1-6.
[2]吴则东,王荣华,张文彬.新时期甜菜糖业面临的机遇、挑战及发展策略[J].中国糖料,2017,39(4):71-73.
[3]李彦丽,马亚怀,柏章才.高配合力甜菜二倍体授粉系Zj401的选育[J].中国糖料,2018,40(6):4-7.
[4]刘升廷,张立明,蔡惠珍,等.甜菜育种动向与展望[J].中国糖料,2016,38(4):56-61.
[5]马龙彪,吴则东,王茂芊,等.甜菜育种的研究进展及未来发展展望[J].中国糖料,2018,40(6):62-65.
[6]张跃彬.我国蔗糖产业安全问题与发展对策[J].中国糖料,2019,41(1):66-68.
[7]陈丽,赵春雷,陈连江,等.甜菜新品种“甜研208”的选育,中国糖料,2013(4):7-8,14.
[8]孙以楚,杨炎生,王华忠,等.甜菜多倍体新品种甜研309的选育,中国糖料,1997(3):1-4.
[9]刘景泉,陈丽,程大友.甜菜多系杂交种甜研八号的选育,中国糖料,1999⑴:1-5.
[10]刘景泉,陈丽,程大友.耐丛根病甜菜杂交种甜研203的选育,中国糖料,1999⑶:9-11.
[11]李彦丽,柏章才,马亚怀.丰产优质抗病甜菜新品种ZM202的选育,中国糖料,2010(3):6-8.
[12]刘景泉,陈丽,程大友.甜菜有粉系轮回选择初报-早代配合力选择,中国甜菜,1993(2):18-24.
[13]陈丽,刘景泉,程大友,等.优质抗病甜菜新品种中甜204的选育,中国糖料,2001(2):1-3.
[14]刘景泉,陈丽.甜菜亲本主要数量性状遗传规律的初步研究,中国甜菜,1984(2):8-19.
[15]Ksenija Taski-Ajdukovic,N Nagl,Zivko Curcic,et al.Estimation of genetic diversity and relationship in sugar beet pollinators based on SSR markers[J].Electronic Journal of Biotechnology,2017,27:1-7.
[16]Monteiro F,Frese L,Castro S,et al.Genetic and Genomic Tools to Asssist Sugar Beet Improvement:The Value of the Crop Wild Relatives[J].Frontiers in Plant Science,2018,9(9):1-8.
[17]Hoffmann C M,Kenter C.Yield Potential of Sugar Beet-Have We Hit the Ceiling?[J].Frontiers in Plant Science,2018,9:1-6.