水解酸化对好氧颗粒污泥形成及脱氮除磷的影响
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摘要
为研究水解酸化对AGS(好氧颗粒污泥)的形成及其脱氮除磷效果的影响,分别采用R1+R2两段式[HUSB(升流式水解酸化池)-SBR]和R3一段式(SBR)方式培养AGS,研究了不同基质下形成的AGS的脱氮除磷效果,并通过高通量测序对水解酸化污泥(R1)和R2、R3运行初期(12 d)与运行后期(97 d)的AGS微生物群落结构进行解析.结果表明,当进水基质为葡萄糖时,R1+R2两段式和R3一段式都能够成功地培养出颗粒致密、沉降性能好的AGS.接种活性污泥后的第13天R2反应器中形成粒径为>1.0~1.6 mm的颗粒污泥,而R3反应器仍以絮状污泥为主;颗粒污泥成熟后,R2中AGS的粒径增长至1.0~2.0 mm间,R3的粒径主要分布在>1.0~1.6 mm间.两种颗粒污泥对COD_(Cr)和NH_3-N均有很好的去除效果,去除率均接近100%.R2对TN和TP的去除率分别为88.5%和72.8%,明显高于R3(67.2%和60.1%);R2出水ρ(TN)最低可达3 mgL,ρ(TP)可达1.1 mgL.高通量测序结果表明,Proteobacteria和Bacteroidetes门始终是主要菌种;随着好氧颗粒污泥的成熟,R2和R3的Shannon-Wiener多样性指数均显著增加;R2中成熟后AGS的Shannon-Wiener多样性指数最高(5.40),表现出良好的生物多样性和系统稳定性.研究表明,水解酸化为AGS的形成提供了关键基质,促进了好氧污泥颗粒化,培养出的AGS运行稳定,脱氮除磷效果好,微生物种群丰富.
In order to testify the impact of hydrolysis and acidification on the formation of aerobic granular sludge(AGS)and its performance of nitrogen and phosphorus removal,a two-stage system(HUSB(R1)+SBR(R2))and a one-stage system(SBR(R3))were set up for comparison of their performance on AGS cultivation on the basis of different substrates.The results showed that both the two-stage system and one-stage system could achieve AGS with dense structure and good settling performance.Granules with average diameters of>1.0-1.6 mm were found in R2 on the 13~(th)day after inoculation,while flocculent sludge was dominant in R3.Granules in R2grew to 1.0-2.0 mm after maturing and granules in R3 at last distributed between 1.0 and 1.6 mm.The removal of COD_(Cr)and NH_3-N in both reactors almost reached 100%and the TN and TP removal in R2 were 88.5%and 72.8%respectively,which outperformed those values of 67.2%for TN and 60.1%for TP in R3.The best effluent quality achieved TN concentration of 3.0 mgL and TP concentration of 1.0 mgL.High-throughput sequencing was applied to analyze the microbial community structure in R2 and R3 in the early and matured stages.Proteobacteria and Bacteroidetes were found the main bacteria throughout the operation.Shannon-Wiener index increased both in R2 and R3 after the maturation of AGS,and the highest value in R2 was 5.4 indicating the best biodiversity and stability.In general,hydrolysis and acidification unit supplied key substrates fond for stable AGS granulation,which gave rise to good nitrogen and phosphorus removal and microbial diversity.
In order to testify the impact of hydrolysis and acidification on the formation of aerobic granular sludge(AGS)and its performance of nitrogen and phosphorus removal,a two-stage system(HUSB(R1)+SBR(R2))and a one-stage system(SBR(R3))were set up for comparison of their performance on AGS cultivation on the basis of different substrates.The results showed that both the two-stage system and one-stage system could achieve AGS with dense structure and good settling performance.Granules with average diameters of>1.0-1.6 mm were found in R2 on the 13~(th)day after inoculation,while flocculent sludge was dominant in R3.Granules in R2grew to 1.0-2.0 mm after maturing and granules in R3 at last distributed between 1.0 and 1.6 mm.The removal of COD_(Cr)and NH_3-N in both reactors almost reached 100%and the TN and TP removal in R2 were 88.5%and 72.8%respectively,which outperformed those values of 67.2%for TN and 60.1%for TP in R3.The best effluent quality achieved TN concentration of 3.0 mgL and TP concentration of 1.0 mgL.High-throughput sequencing was applied to analyze the microbial community structure in R2 and R3 in the early and matured stages.Proteobacteria and Bacteroidetes were found the main bacteria throughout the operation.Shannon-Wiener index increased both in R2 and R3 after the maturation of AGS,and the highest value in R2 was 5.4 indicating the best biodiversity and stability.In general,hydrolysis and acidification unit supplied key substrates fond for stable AGS granulation,which gave rise to good nitrogen and phosphorus removal and microbial diversity.
引文
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[14]ZHENG Yuming,YU Hanqing,SHENG Guoping.Physical and chemical characteristics of granular activated sludge from a sequencing batch airlift reactor[J].Process Biochemistry,2005,40(2):645-650.
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[18]PRONK M,ABBAS B,AL-ZUHAIRY S H K,et al.Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge[J].Applied Microbiology and Biotechnology,2015,99(12):5257-5268.
[19]TAY J H,LIU Q S,LIU Y.Characteristics of aerobic granules grown on glucose and acetate in sequential aerobic sludge blanket reactors[J].Environmental Technology,2002,23(8):931-936.
[20]PRONK M,ABBAS B,KLEEREBEZEM R,et al.Effect of sludge age on methanogenic and glycogen accumulating organisms in an aerobic granular sludge process fed with methanol and acetate[J].Microbial Biotechnology,2015,8(5):853-864.
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[23]CARTA F,BEUN J J,VAN LOOSDRECHT M C M,et al.Simultaneous storage and degradation of phb and glycogen in activated sludge cultures[J].Water Research,2001,35(11):2693-2701.
[24]DE KREUK M K,VAN LOOSDRECHT M C M.Formation of aerobic granules with domestic sewage[J].Journal of Environmental Engineering-Asce,2006,132(6):694-697.
[25]DE KREUK M K,VAN LOOSDRECHT M C M.Selection of slow growing organisms as a means for improving aerobic granular sludge stability[J].Water Science and Technology,2004,49(1112):9-17.
[26]ZHENG Mingxia,ZHENG Mingyue,WU Yuanyuan,et al.Effect of p H on types of acidogenic fermentation of fruit and vegetable wastes[J].Biotechnology and Bioprocess Engineering,2015,20(2):298-303.
[27]NI Bingjie,XIE Wenming,LIU Shaogen,et al.Granulation of activated sludge in a pilot-scale sequencing batch reactor for the treatment of low-strength municipal wastewater[J].Water Research,2009,43(3):751-761.
[28]曾薇,李磊,杨莹莹,等.A2O工艺处理生活污水短程硝化反硝化的研究[J].中国环境科学,2010,30(5):625-632.ZENG Wei,LI Lei,YANG,Yingying,et al.Short-cut nitrification and denitrification in A2O process treating domestic wastewater[J].China Environmental Science,2010,30(5):625-632.
[29]郑燕清,周建华.除磷工艺中厌氧释磷和好氧吸磷的影响因素[J].中国市政工程,2007(1):48-50.
[30]杨冰.两种碳源条件下好氧颗粒污泥中细菌的群落结构研究[D].武汉:武汉理工大学,2015.
[31]戴昕.好氧颗粒污泥工艺运行过程重要功能菌群研究[D].杭州:浙江大学,2014.
[2]LIU Y,TAY J H.State of the art of biogranulation technology for wastewater treatment[J].Biotechnology Advances,2004,22(7):533-563.
[3]SHOW K Y,LEE D J,TAY J H.Aerobic granulation:advances and challenges[J].Applied Biochemistry and Biotechnology,2012,167(6):1622-1640.
[4]BEUN J J,VAN LOOSDRECHT M C M,HEIJNEN J J.Aerobic granulation[J].Water Science and Technology,2000,41(45):41-48.
[5]BASSIN J P,KLEEREBEZEM R,DEZOTTI M,et al.Simultaneous nitrogen and phosphate removal in aerobic granular sludge reactors operated at different temperatures[J].Water Research,2012,46(12):3805-3816.
[6]WANG Shuguang,LIU Xianwei,GONG Wenxin,et al.Aerobic granulation with brewery wastewater in a sequencing batch reactor[J].Bioresource Technology,2007,98(11):2142-2147.
[7]YU Kuizu,YU Hanqing.Formation and characterization of aerobic granules in a sequencing batch reactor treating soybean-processing wastewater[J].Environmental Science&Technology,2005,39:2818-2827.
[8]LIU Li,SHENG Guoping,LI Wenwei,et al.Cultivation of aerobic granular sludge with a mixed wastewater rich in toxic organics[J].Biochemical Engineering Journal,2011,57:7-12.
[9]PRONK M,DE KREUK M K,DE BRUIN B,et al.Full scale performance of the aerobic granular sludge process for sewage treatment[J].Water Research,2015,84:207-217.
[10]DUQUE A F,BESSA V S,CARVALHO M F,et al.2-fluorophenol degradation by aerobic granular sludge in a sequencing batch reactor[J].Water Research,2011,45(20):6745-6752.
[11]LIU Yongqiang,MOY B Y,TAY J H.COD removal and nitrification of low-strength domestic wastewater in aerobic granular sludge sequencing batch reactors[J].Enzyme and Microbial Technology,2007,42(1):23-28.
[12]LIU Yongqiang,KONG Yunhua,TAY J H,et al.Enhancement of start-up of pilot-scale granular SBR fed with real wastewater[J].Separation and Purification Technology,2011,82:190-196.
[13]ZHENG Yuming,YU Hanqing,LIU Shuangjiang,et al.Formation and instability of aerobic granules under high organic loading conditions[J].Chemosphere,2006,63(10):1791-1800.
[14]ZHENG Yuming,YU Hanqing,SHENG Guoping.Physical and chemical characteristics of granular activated sludge from a sequencing batch airlift reactor[J].Process Biochemistry,2005,40(2):645-650.
[15]LIU Yongqiang,MOY Benjamin,KONG Yunhua,et al.Formation,physical characteristics and microbial community structure of aerobic granules in a pilot-scale sequencing batch reactor for real wastewater treatment[J].Enzyme and Microbial Technology,2010,46(6):520-525.
[16]MOSQUERA-CORRAL A,DE KREUK M K,HEIJNEN J J,et al.Effects of oxygen concentration on N-removal in an aerobic granular sludge reactor[J].Water Research,2005,39(12):2676-2686.
[17]DE KREUK M K,PRONK M,VAN LOOSDRECHT M C.Formation of aerobic granules and conversion processes in an aerobic granular sludge reactor at moderate and low temperatures[J].Water Research,2005,39(18):4476-4484.
[18]PRONK M,ABBAS B,AL-ZUHAIRY S H K,et al.Effect and behaviour of different substrates in relation to the formation of aerobic granular sludge[J].Applied Microbiology and Biotechnology,2015,99(12):5257-5268.
[19]TAY J H,LIU Q S,LIU Y.Characteristics of aerobic granules grown on glucose and acetate in sequential aerobic sludge blanket reactors[J].Environmental Technology,2002,23(8):931-936.
[20]PRONK M,ABBAS B,KLEEREBEZEM R,et al.Effect of sludge age on methanogenic and glycogen accumulating organisms in an aerobic granular sludge process fed with methanol and acetate[J].Microbial Biotechnology,2015,8(5):853-864.
[21]DE KREUK M K,HEIJNEN J J,VAN LOOSDRECHT M C.Simultaneous COD,nitrogen,and phosphate removal by aerobic granular sludge[J].Biotechnology and Bioengineering,2005,90(6):761-769.
[22]WINKLER M K,BASSIN J P,KLEEREBEZEM R,et al.Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO-GAO competition at high temperatures[J].Water Research,2011,45(11):3291-3299.
[23]CARTA F,BEUN J J,VAN LOOSDRECHT M C M,et al.Simultaneous storage and degradation of phb and glycogen in activated sludge cultures[J].Water Research,2001,35(11):2693-2701.
[24]DE KREUK M K,VAN LOOSDRECHT M C M.Formation of aerobic granules with domestic sewage[J].Journal of Environmental Engineering-Asce,2006,132(6):694-697.
[25]DE KREUK M K,VAN LOOSDRECHT M C M.Selection of slow growing organisms as a means for improving aerobic granular sludge stability[J].Water Science and Technology,2004,49(1112):9-17.
[26]ZHENG Mingxia,ZHENG Mingyue,WU Yuanyuan,et al.Effect of p H on types of acidogenic fermentation of fruit and vegetable wastes[J].Biotechnology and Bioprocess Engineering,2015,20(2):298-303.
[27]NI Bingjie,XIE Wenming,LIU Shaogen,et al.Granulation of activated sludge in a pilot-scale sequencing batch reactor for the treatment of low-strength municipal wastewater[J].Water Research,2009,43(3):751-761.
[28]曾薇,李磊,杨莹莹,等.A2O工艺处理生活污水短程硝化反硝化的研究[J].中国环境科学,2010,30(5):625-632.ZENG Wei,LI Lei,YANG,Yingying,et al.Short-cut nitrification and denitrification in A2O process treating domestic wastewater[J].China Environmental Science,2010,30(5):625-632.
[29]郑燕清,周建华.除磷工艺中厌氧释磷和好氧吸磷的影响因素[J].中国市政工程,2007(1):48-50.
[30]杨冰.两种碳源条件下好氧颗粒污泥中细菌的群落结构研究[D].武汉:武汉理工大学,2015.
[31]戴昕.好氧颗粒污泥工艺运行过程重要功能菌群研究[D].杭州:浙江大学,2014.