汉黄芩素对人髓母细胞瘤增殖和凋亡的影响
|
摘要
目的:探讨汉黄芩素对髓母细胞瘤增殖和凋亡的影响。方法:运用CCK-8(cell counting kit-8)试剂盒,流式细胞仪检测不同浓度汉黄芩素及同一浓度不同作用时间对髓母细胞瘤细胞活性及凋亡的影响;通过比色法检测汉黄芩素对髓母细胞瘤细胞中Caspase-3蛋白活性的影响;通过体内试验,检测汉黄芩素在裸鼠成瘤实验中对髓母细胞瘤细胞活性的影响。结果:汉黄芩素可以抑制髓母细胞瘤细胞活性并诱导髓母细胞瘤细胞凋亡。汉黄芩素抑制髓母细胞瘤细胞活性具有浓度依赖性和时间依赖性。此外,汉黄芩素可增强髓母细胞瘤细胞中凋亡蛋白Caspase-3的活性,诱导髓母细胞瘤细胞凋亡。在裸鼠成瘤实验中,汉黄芩素可抑制髓母细胞瘤的体内增殖。结论:汉黄芩素在体外可抑制髓母细胞瘤细胞活性及诱导细胞凋亡。在裸鼠成瘤实验中,汉黄芩素可以抑制肿瘤增长。
Objective:To explore the impact of wogonin on the proliferation and apoptosis of medulloblastoma cell line DAOY.Methods:DAOY cells were divided into experimental groups(added with wogonin at difference concentrations)and control group. The proliferation of DAOY was detected using CCK-8 assay kit.The apoptosis was analyzed by Annexin Ⅴ-FITC/PI double staining and the activity of caspase-3 was determined via colorimetry. Moreover,we also detected the tumor inhibiting function of wogonin in vivo using subcutaneous tumor model. Results:Wognin remarkably inhibited the proliferation of medulloblastoma cell DAOY and induced the apoptosis in a dose and time dependent manner. The apoptosis was characterized by the increase of apoptosis protein caspase-3.Conclusion:Wogonin could inhibit medulloblastoma cell proliferation both in vitro and in vivo via inducing medulloblastoma cells apoptosis.
Objective:To explore the impact of wogonin on the proliferation and apoptosis of medulloblastoma cell line DAOY.Methods:DAOY cells were divided into experimental groups(added with wogonin at difference concentrations)and control group. The proliferation of DAOY was detected using CCK-8 assay kit.The apoptosis was analyzed by Annexin Ⅴ-FITC/PI double staining and the activity of caspase-3 was determined via colorimetry. Moreover,we also detected the tumor inhibiting function of wogonin in vivo using subcutaneous tumor model. Results:Wognin remarkably inhibited the proliferation of medulloblastoma cell DAOY and induced the apoptosis in a dose and time dependent manner. The apoptosis was characterized by the increase of apoptosis protein caspase-3.Conclusion:Wogonin could inhibit medulloblastoma cell proliferation both in vitro and in vivo via inducing medulloblastoma cells apoptosis.
引文
[1] Packer RJ,Macdonald T,Vezina G,et al. Medulloblastoma and primitive neuroectodermal tumors[J]. Handb Clin Neurol,2012,105:529-548
[2] Saury JM,Emanuelson I. Cognitive consequences of the treatment of medulloblastoma among children[J]. Pediatr Neurol,2011,44(1):21-30
[3] Lucas CD,Dorward DA,Sharma S,et al. Wogonin induces eosinophil apoptosis and attenuates allergic airway inflammation[J]. Am J Respir Crit Care Med,2015,191(6):626-636
[4] Khan S,Zhang D,Zhang Y,et al. Wogonin attenuates diabetic cardiomyopathy through its anti-inflammatory and anti-oxidative properties[J]. Mol Cell Endocrinol,2016,428:101-108
[5] Fu P,Du F,Liu Y,et al. Wogonin increases doxorubicin sensitivity by down-regulation of IGF-1R/AKT signaling pathway in human breast cancer[J]. Cell Mol Biol(Noisyle-grand),2015,61(7):123-127
[6] Hu C,Xu M,Qin R,et al. Wogonin induces apoptosis and endoplasmic reticulum stress in HL-60 leukemia cells through inhibition of the PI3K-AKT signaling pathway[J]. Oncol Rep,2015,33(6):3146-3154
[7] Gudrunardottir T,Lannering B,Remke M,et al. Treatment developments and the unfolding of the quality of life discussion in childhood medulloblastoma:a review[J].Childs Nerv Syst,2014,30(6):979-990
[8] Yang L,Wang Q,Li D,et al. Wogonin enhances antitumor activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo through ROS-mediated downregulation of cFLIPL and IAP proteins[J]. Apoptosis,2013,18(5):618-626
[9] Wu X,Liu P,Zhang H,et al. Wogonin as a targeted therapeutic agent for EBV(+)lymphoma cells involved in LMP1/NF-κB/miR-155/PU.1 pathway[J]. BMC Cancer,2017,17(1):147
[10]Kim EH,Jang H,Shin D,et al. Targeting Nrf2 with wogonin overcomes cisplatin resistance in head and neck cancer[J]. Apoptosis,2016,21(11):1265-1278
[11]Baek JS,Na YG,Cho CW. Sustained cytotoxicity of wogonin on breast cancer cells by encapsulation in solid lipid nanoparticles[J]. Nanomaterials(Basel),2018,8(3):159
[12]苏飞,赵达,庄亚飞,等.汉黄芩素对肝癌细胞SMMC-7721增殖、迁移及侵袭的影响[J].中华实用诊断与治疗杂志,2017,31(8):735-738
[13]陈望昊,王慧博,刘宁.汉黄芩素调节恶性胶质瘤U87细胞增殖和凋亡并影响miR-128的表达[J].南京医科大学学报(自然科学版),2013,33(4):456-461
[2] Saury JM,Emanuelson I. Cognitive consequences of the treatment of medulloblastoma among children[J]. Pediatr Neurol,2011,44(1):21-30
[3] Lucas CD,Dorward DA,Sharma S,et al. Wogonin induces eosinophil apoptosis and attenuates allergic airway inflammation[J]. Am J Respir Crit Care Med,2015,191(6):626-636
[4] Khan S,Zhang D,Zhang Y,et al. Wogonin attenuates diabetic cardiomyopathy through its anti-inflammatory and anti-oxidative properties[J]. Mol Cell Endocrinol,2016,428:101-108
[5] Fu P,Du F,Liu Y,et al. Wogonin increases doxorubicin sensitivity by down-regulation of IGF-1R/AKT signaling pathway in human breast cancer[J]. Cell Mol Biol(Noisyle-grand),2015,61(7):123-127
[6] Hu C,Xu M,Qin R,et al. Wogonin induces apoptosis and endoplasmic reticulum stress in HL-60 leukemia cells through inhibition of the PI3K-AKT signaling pathway[J]. Oncol Rep,2015,33(6):3146-3154
[7] Gudrunardottir T,Lannering B,Remke M,et al. Treatment developments and the unfolding of the quality of life discussion in childhood medulloblastoma:a review[J].Childs Nerv Syst,2014,30(6):979-990
[8] Yang L,Wang Q,Li D,et al. Wogonin enhances antitumor activity of tumor necrosis factor-related apoptosis-inducing ligand in vivo through ROS-mediated downregulation of cFLIPL and IAP proteins[J]. Apoptosis,2013,18(5):618-626
[9] Wu X,Liu P,Zhang H,et al. Wogonin as a targeted therapeutic agent for EBV(+)lymphoma cells involved in LMP1/NF-κB/miR-155/PU.1 pathway[J]. BMC Cancer,2017,17(1):147
[10]Kim EH,Jang H,Shin D,et al. Targeting Nrf2 with wogonin overcomes cisplatin resistance in head and neck cancer[J]. Apoptosis,2016,21(11):1265-1278
[11]Baek JS,Na YG,Cho CW. Sustained cytotoxicity of wogonin on breast cancer cells by encapsulation in solid lipid nanoparticles[J]. Nanomaterials(Basel),2018,8(3):159
[12]苏飞,赵达,庄亚飞,等.汉黄芩素对肝癌细胞SMMC-7721增殖、迁移及侵袭的影响[J].中华实用诊断与治疗杂志,2017,31(8):735-738
[13]陈望昊,王慧博,刘宁.汉黄芩素调节恶性胶质瘤U87细胞增殖和凋亡并影响miR-128的表达[J].南京医科大学学报(自然科学版),2013,33(4):456-461