酵母硒对分娩应激状态下围产期奶牛代谢的影响
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摘要
【目的】研究酵母硒对围产期奶牛代谢的影响,从代谢物变化的角度阐述其在分娩应激状态下的生物学作用。【方法】选择24头体况、胎次、年龄、体质量和预产期相近的健康围产前期奶牛随机分为对照组和试验组,每组各12头,对照组饲喂基础日粮,试验组在基础日粮中添加0.15g/kg干物质的酵母硒,于产前21d开始饲喂,分娩后当天停止饲喂。在产前21d及分娩后6h内尾静脉采血,使用EDTA抗凝并离心收集血浆。应用UPLC-TOF/MS分析技术,在正负离子模式下对所有血浆样品进行代谢组学分析,数据经预处理后利用多维统计(包括主成分分析(principal component analysis,PCA)和正交偏最小二乘法判别分析(orthogonal partial least squares-discriminantanalysis,OPLS-DA))和单变量(t检验和差异倍数)分析方法,分析在产前21d和分娩当天试验组和对照组奶牛血浆中代谢物差异性,以OPLS-DA获得的VIP值>1和t检验中P<0.1为条件筛选差异代谢物。【结果】2组奶牛血浆代谢物在饲喂硒前差异不明显,饲喂硒后有19种差异代谢物。与对照组相比,试验组奶牛在分娩后血浆中L-苯丙氨酸、L-亮氨酸、异亮氨酸、L-谷氨酰胺、L-缬氨酸、酪胺、酮亮氨酸和硫酸吲哚酚显著降低(P<0.05),甜菜碱、7-甲基黄嘌呤、马尿酸、尿囊素和柠檬酸显著升高(P<0.05),L-正亮氨酸、DL-苯丙氨酸、乙二醇单乙醚和氧代己二酸呈降低趋势(0.05 【Objective】The aim of this study was to detect the effect of selenium yeast on metabolism of transition dairy cows and to explain the biological functions of selenium yeast during parturition stress.【Method】A total of 24 transition dairy cows with similar body condition scoring,parity,age and due date were selected and randomly divided into a test group and a control group.The 12 cows in each group were fed on basal diet supplemented with or without 0.15 g selenium yeast per kg dry matter(DM)from 21 days prepartum to postpartum.Plasma from caudal vein was collected 21 dprepartum and 6 hpostpartum.Serum samples were subjected to metabolic profiling analysis using ultra performance liquid chromatography time-of-flight mass spectrometry(UPLC-TOF/MS)in positive and negative modes.Pretreatment data was performed by multivariate statistical methods including principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)as well as univariate analysis including ttest or fold difference method.The differences of plasma metabolites were analyzed between test group and control group 21 dprepartum and the delivery day.The differential metabolites were selected based on the VIP score(>1)obtained form OPLS-DA model and P<0.1 in t-test.【Result】The plasma metabolites of two groups had no significant difference before feeding selenium,but there were 19 differential metabolites after feeding selenium.Compared with control group,L-phenylalanine,L-leucine,isoleucine,L-glutamine,L-valine,tyramine,leucine,and indoxyl sulfate in test group were significantly reduced(P<0.05),while betaine,7-methylxanthine,hippuric acid,allantoin and citrate were significantly increased(P<0.05).Lnorleucine,DL-phenylalanine,2-ethoxyethanol and 2-oxoadipic acid in test group were lower than that of the control(0.05
引文
[1]李鹏,龙淼,张燚,等.围产期奶牛炎症反应与营养代谢性疾病[J].中国兽医杂志,2014,50(5):62-64.Li P,Long M,Zhang Y,et al.Inflammation response and nutrition metabolized disease of transition dairy cows[J].Chines Journal of Veterinary Medicine,2014,50(5):62-64.
[2]Bradford B J,Yuan K,Farney J K,et al.Invited review:inflammation during the transition to lactation:new adventures with an old flame[J].Journal of Dairy Science,2015,98(10):6631-6650.
[3]Contreras G A,O’Boyle N J,Herdt T H,et al.Lipomobilization in periparturient dairy cows influences the composition of plasma nonesterified fatty acids and leukocyte phospholipid fatty acids[J].Journal of Dairy Science,2010,93(6):2508-2516.
[4]Mehdi Y,Dufrasne I.Selenium in cattle:a review[J].Molecules,2016,21(4):545.
[5]Weiss W P,Hogan J S.Effect of selenium source on selenium status,neutrophil function,and response to intramammary endotoxin challenge of dairy cows[J].Journal of Dairy Science,2005,88(12):4366.
[6]Rutigliano H M,Lima F S,Cerri R L A,et al.Effects of method of presynchronization and source of selenium on uterine health and reproduction in dairy cows[J].Journal of Dairy Science,2008,91(9):3323-3336.
[7]Wei Z,Yao M,Li Y,et al.Dietary selenium deficiency exacerbates lipopolysaccharide-induced inflammatory response in mouse mastitis models[J].Inflammation,2014,37(6):1925-1931.
[8]National Research Council(USA).Nutrient requirements of dairy cattle[M].Washington D C:National Academies Press,2001:141-142.
[9]Zhao Z,Barcus M,Kim J,et al.High dietary selenium intake alters lipid metabolism and protein synthesis in liver and muscle of pigs[J].Journal of Nutrition,2016,146(9):1625-1633.
[10]雒国彬,赵洪波,文奇男,等.酵母硒对围产前期奶牛全血硒含量和血液抗氧化能力的影响[J].中国饲料,2010(16):34-36.Luo G B,Zhao H B,Wen Q N,et al.Effects of diet supplementation with Se-yeast on blood Se contents and anti-oxidation performance[J].China Feed,2010(16):34-36.
[11]Sies H,Berndt C,Jones D P.Oxidative stress[J].Annual Review of Biochemistry,2017,86(10):715.
[12]Benton H P,Ivanisevic J,Mahieu N,et al.Autonomous metabolomics for rapid metabolite identification global profiling[J].Analytical Chemistry,2015,87(2):884-891.
[13]弓剑,晓敏.围产期奶牛炎症反应及其与免疫和能量代谢的关系[J].动物营养学报,2016,28(9):2667-2672.Gong J,Xiao M.Inflammation periparturient dairy cows and its relationship immunity and energy metabolism[J].Chinese Journal of Animal Nutrition,2016,28(9):2667-2672.
[14]Renko K,Hofmann P J,Stoedter M,et al.Down-regulation of the hepatic selenoprotein biosynthesis machinery impairs selenium metabolism during the acute phase response in mice[J].Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology,2009,23(6):1758.
[15]Sun L W,Zhang H Y,Wu L,et al.1 H-Nuclear magnetic resonance-based plasma metabolic profiling of dairy cows with clinical and subclinical ketosis[J].Journal of Dairy Science,2014,97(3):1552-1562.
[16]胡秀丽,李体远,姜熙罗,等.多不饱和脂肪酸对饲低硒粮大鼠血浆游离脂肪酸的影响[J].中国地方病防治杂志,1998(4):204-205.Hu X L,Li T Y,Jiang X L,et al.Effect of polyunsaturated fatty acids on free fatty acids of rats fed on selenium deficient cereals[J].Chinese Journal of Control of Endemic Disease,1998(4):204-205.
[17]张鹏春,陈丽君,范瑞威,等.亚硒酸钠保护游离脂肪酸诱导肝细胞功能和Caspase 3活性的变化研究[J].中国饲料,2013(14):15-18.Zhang P C,Chen L J,Fan R W,et al.Effect of sodium selenite on liver function and the activity of Caspase 3induced by free fatty acids[J].China Feed,2013(14):15-18.
[18]王刚.基于LC/MS联用技术的产后卵巢静止奶牛血浆代谢组学分析[D].黑龙江大庆:黑龙江八一农垦大学,2017.Wang G.Plasma metabolomics analysis of postpartum inactive ovaries in dairy cows based on liquid chromatography/mass spectrometry technology[D].Daqing,Heilongjiang:Heilongjiang Bayi Agricultural University,2017.
[19]Haque M N,Rulquin H,Lemosquet S.Milk protein responses in dairy cows to changes in postruminal supplies of arginine,isoleucine,and valine[J].Journal of Dairy Science,2013,96(1):420-430.
[20]苏伟鹏,徐稳,张昊,等.日粮亮氨酸水平与宫内发育迟缓对断奶仔猪肝脏抗氧化功能的影响[J].食品工业科技,2016,37(19):345-351.Su W P,Xu W,Zhang H,et al.Effect of dietary concentrations of leucine and intrauterine growth retardation on antioxidant function in the liver of weaned piglets[J].Science and Technology of Food Industry,2016,37(19):345-351.
[21]张军民,王连递,高振川,等.日粮添加谷氨酰胺对早期断奶仔猪抗氧化能力的影响[J].畜牧兽医学报,2002,33(2):105-109.Zhang J M,Wang L D,Gao Z C,et al.Effect of dietary glutamine supplementation on antioxidant capacity of tissue in the early-weaned piglets[J].Chinese Journal of Animal and Veterinary Sciences,2002,33(2):105-109.
[22]Fleisherberkovich S,Abramovitchdahan C,Benshabat S,et al.Inhibitory effect of carnosine and N-acetyl carnosine on LPS-induced microglial oxidative stress and inflammation[J].Peptides,2009,30(7):1306-1312.
[23]许宗运,张丽娟,韩俊文.不同水平酵母硒对奶牛血液抗氧化能力的影响[J].动物营养学报,2007,19(6):753-757.Xu Z Y,Zhang L J,Han J W.Effects of Se-yeast in dairy ration on somatic cell and anti-oxidation performance[J].Chinese Journal of Animal Nutrition,2007,19(6):753-757.
[24]Yang K,Xu X,Nie L,et al.Indoxyl sulfate induces oxidative stress and hypertrophy in cardiomyocytes by inhibiting the AMPK/UCP2signaling pathway[J].Toxicology Letters,2015,234(2):110.
[25]Shimizu H,Bolati D,Higashiyama Y,et al.Indoxyl sulfate upregulates renal expression of MCP-1via production of ROSand activation of NF-κB,p53,ERK,and JNK in proximal tubular cells[J].Life Sciences,2012,90(13/14):525-530.
[26]Westergaard N,Waagepetersen H S,Belhage B,et al.Citrate,a ubiquitous key metabolite with regulatory function in the CNS[J].Neurochemical Research,2017,42(6):1583.
[27]李卓卿.有机酸及甜味剂组合对奶牛生产性能的影响[D].吉林长春:吉林农业大学,2005.Li Z Q.Effect of supplement organic acids and sweeteners on production performance of dairy cows[D].Changchun,Jilin:Jilin Agricultural University,2005.
[28]朱玉山,徐辉碧,黄开勋,等.亚硒酸钠对大鼠代谢产物影响的核磁共振研究[J].分析科学学报,2004,20(1):44-47.Zhu Y S,Xu H B,Huang K X,et al.Effect of selenite at ULon the metabolites in rat urine by 1 H NMR spectroscopy[J].Journal of Analytical Science,2004,20(1):44-47.
[29]Day C R,Kempson S A.Betaine chemistry,roles,and potential use in liver disease[J].Biochim Biophys Acta,2016,1860(6):1098-1106.
[30]Ahn M,Park J S,Chae S,et al.Hepatoprotective effects of lycium chinense miller fruit and its constituent betaine in CCl4-induced hepatic damage in rats[J].Acta Histochemica,2014,116(6):1104-1112.
[31]Monteiro A P A,Bernard J K,Guo J R,et al.Effects of feeding betaine-containing liquid supplement to transition dairy cows[J].Journal of Dairy Science,2016,100(2):1063-1071.
[32]张拴林,袁霞,徐亚光,等.硒和维生素E对西门塔尔牛瘤胃发酵和尿嘌呤衍生物含量的影响[J].动物营养学报,2013,25(2):326-333.Zhang S L,Yuan X,Xu Y G,et al.Effects of selenium and vitamin E on rumen fermentation and urinary purine derivative contents in simmental steers[J].Chinese Journal of Animal Nutrition,2013,25(2):326-333.
[33]张丽娟,姚鹃,谭斌,等.奶牛日粮中不同水平酵母硒对表观消化率的影响[J].中国畜牧兽医,2007,34(8):66-68.Zhang L J,Yao J,Tan B,et al.Effects of different supplementary concentration Se-yeast in dairy ration on digestibility[J].China Animal Husbandry&Veterinary Medicine,2007,34(8):66-68.
[2]Bradford B J,Yuan K,Farney J K,et al.Invited review:inflammation during the transition to lactation:new adventures with an old flame[J].Journal of Dairy Science,2015,98(10):6631-6650.
[3]Contreras G A,O’Boyle N J,Herdt T H,et al.Lipomobilization in periparturient dairy cows influences the composition of plasma nonesterified fatty acids and leukocyte phospholipid fatty acids[J].Journal of Dairy Science,2010,93(6):2508-2516.
[4]Mehdi Y,Dufrasne I.Selenium in cattle:a review[J].Molecules,2016,21(4):545.
[5]Weiss W P,Hogan J S.Effect of selenium source on selenium status,neutrophil function,and response to intramammary endotoxin challenge of dairy cows[J].Journal of Dairy Science,2005,88(12):4366.
[6]Rutigliano H M,Lima F S,Cerri R L A,et al.Effects of method of presynchronization and source of selenium on uterine health and reproduction in dairy cows[J].Journal of Dairy Science,2008,91(9):3323-3336.
[7]Wei Z,Yao M,Li Y,et al.Dietary selenium deficiency exacerbates lipopolysaccharide-induced inflammatory response in mouse mastitis models[J].Inflammation,2014,37(6):1925-1931.
[8]National Research Council(USA).Nutrient requirements of dairy cattle[M].Washington D C:National Academies Press,2001:141-142.
[9]Zhao Z,Barcus M,Kim J,et al.High dietary selenium intake alters lipid metabolism and protein synthesis in liver and muscle of pigs[J].Journal of Nutrition,2016,146(9):1625-1633.
[10]雒国彬,赵洪波,文奇男,等.酵母硒对围产前期奶牛全血硒含量和血液抗氧化能力的影响[J].中国饲料,2010(16):34-36.Luo G B,Zhao H B,Wen Q N,et al.Effects of diet supplementation with Se-yeast on blood Se contents and anti-oxidation performance[J].China Feed,2010(16):34-36.
[11]Sies H,Berndt C,Jones D P.Oxidative stress[J].Annual Review of Biochemistry,2017,86(10):715.
[12]Benton H P,Ivanisevic J,Mahieu N,et al.Autonomous metabolomics for rapid metabolite identification global profiling[J].Analytical Chemistry,2015,87(2):884-891.
[13]弓剑,晓敏.围产期奶牛炎症反应及其与免疫和能量代谢的关系[J].动物营养学报,2016,28(9):2667-2672.Gong J,Xiao M.Inflammation periparturient dairy cows and its relationship immunity and energy metabolism[J].Chinese Journal of Animal Nutrition,2016,28(9):2667-2672.
[14]Renko K,Hofmann P J,Stoedter M,et al.Down-regulation of the hepatic selenoprotein biosynthesis machinery impairs selenium metabolism during the acute phase response in mice[J].Faseb Journal Official Publication of the Federation of American Societies for Experimental Biology,2009,23(6):1758.
[15]Sun L W,Zhang H Y,Wu L,et al.1 H-Nuclear magnetic resonance-based plasma metabolic profiling of dairy cows with clinical and subclinical ketosis[J].Journal of Dairy Science,2014,97(3):1552-1562.
[16]胡秀丽,李体远,姜熙罗,等.多不饱和脂肪酸对饲低硒粮大鼠血浆游离脂肪酸的影响[J].中国地方病防治杂志,1998(4):204-205.Hu X L,Li T Y,Jiang X L,et al.Effect of polyunsaturated fatty acids on free fatty acids of rats fed on selenium deficient cereals[J].Chinese Journal of Control of Endemic Disease,1998(4):204-205.
[17]张鹏春,陈丽君,范瑞威,等.亚硒酸钠保护游离脂肪酸诱导肝细胞功能和Caspase 3活性的变化研究[J].中国饲料,2013(14):15-18.Zhang P C,Chen L J,Fan R W,et al.Effect of sodium selenite on liver function and the activity of Caspase 3induced by free fatty acids[J].China Feed,2013(14):15-18.
[18]王刚.基于LC/MS联用技术的产后卵巢静止奶牛血浆代谢组学分析[D].黑龙江大庆:黑龙江八一农垦大学,2017.Wang G.Plasma metabolomics analysis of postpartum inactive ovaries in dairy cows based on liquid chromatography/mass spectrometry technology[D].Daqing,Heilongjiang:Heilongjiang Bayi Agricultural University,2017.
[19]Haque M N,Rulquin H,Lemosquet S.Milk protein responses in dairy cows to changes in postruminal supplies of arginine,isoleucine,and valine[J].Journal of Dairy Science,2013,96(1):420-430.
[20]苏伟鹏,徐稳,张昊,等.日粮亮氨酸水平与宫内发育迟缓对断奶仔猪肝脏抗氧化功能的影响[J].食品工业科技,2016,37(19):345-351.Su W P,Xu W,Zhang H,et al.Effect of dietary concentrations of leucine and intrauterine growth retardation on antioxidant function in the liver of weaned piglets[J].Science and Technology of Food Industry,2016,37(19):345-351.
[21]张军民,王连递,高振川,等.日粮添加谷氨酰胺对早期断奶仔猪抗氧化能力的影响[J].畜牧兽医学报,2002,33(2):105-109.Zhang J M,Wang L D,Gao Z C,et al.Effect of dietary glutamine supplementation on antioxidant capacity of tissue in the early-weaned piglets[J].Chinese Journal of Animal and Veterinary Sciences,2002,33(2):105-109.
[22]Fleisherberkovich S,Abramovitchdahan C,Benshabat S,et al.Inhibitory effect of carnosine and N-acetyl carnosine on LPS-induced microglial oxidative stress and inflammation[J].Peptides,2009,30(7):1306-1312.
[23]许宗运,张丽娟,韩俊文.不同水平酵母硒对奶牛血液抗氧化能力的影响[J].动物营养学报,2007,19(6):753-757.Xu Z Y,Zhang L J,Han J W.Effects of Se-yeast in dairy ration on somatic cell and anti-oxidation performance[J].Chinese Journal of Animal Nutrition,2007,19(6):753-757.
[24]Yang K,Xu X,Nie L,et al.Indoxyl sulfate induces oxidative stress and hypertrophy in cardiomyocytes by inhibiting the AMPK/UCP2signaling pathway[J].Toxicology Letters,2015,234(2):110.
[25]Shimizu H,Bolati D,Higashiyama Y,et al.Indoxyl sulfate upregulates renal expression of MCP-1via production of ROSand activation of NF-κB,p53,ERK,and JNK in proximal tubular cells[J].Life Sciences,2012,90(13/14):525-530.
[26]Westergaard N,Waagepetersen H S,Belhage B,et al.Citrate,a ubiquitous key metabolite with regulatory function in the CNS[J].Neurochemical Research,2017,42(6):1583.
[27]李卓卿.有机酸及甜味剂组合对奶牛生产性能的影响[D].吉林长春:吉林农业大学,2005.Li Z Q.Effect of supplement organic acids and sweeteners on production performance of dairy cows[D].Changchun,Jilin:Jilin Agricultural University,2005.
[28]朱玉山,徐辉碧,黄开勋,等.亚硒酸钠对大鼠代谢产物影响的核磁共振研究[J].分析科学学报,2004,20(1):44-47.Zhu Y S,Xu H B,Huang K X,et al.Effect of selenite at ULon the metabolites in rat urine by 1 H NMR spectroscopy[J].Journal of Analytical Science,2004,20(1):44-47.
[29]Day C R,Kempson S A.Betaine chemistry,roles,and potential use in liver disease[J].Biochim Biophys Acta,2016,1860(6):1098-1106.
[30]Ahn M,Park J S,Chae S,et al.Hepatoprotective effects of lycium chinense miller fruit and its constituent betaine in CCl4-induced hepatic damage in rats[J].Acta Histochemica,2014,116(6):1104-1112.
[31]Monteiro A P A,Bernard J K,Guo J R,et al.Effects of feeding betaine-containing liquid supplement to transition dairy cows[J].Journal of Dairy Science,2016,100(2):1063-1071.
[32]张拴林,袁霞,徐亚光,等.硒和维生素E对西门塔尔牛瘤胃发酵和尿嘌呤衍生物含量的影响[J].动物营养学报,2013,25(2):326-333.Zhang S L,Yuan X,Xu Y G,et al.Effects of selenium and vitamin E on rumen fermentation and urinary purine derivative contents in simmental steers[J].Chinese Journal of Animal Nutrition,2013,25(2):326-333.
[33]张丽娟,姚鹃,谭斌,等.奶牛日粮中不同水平酵母硒对表观消化率的影响[J].中国畜牧兽医,2007,34(8):66-68.Zhang L J,Yao J,Tan B,et al.Effects of different supplementary concentration Se-yeast in dairy ration on digestibility[J].China Animal Husbandry&Veterinary Medicine,2007,34(8):66-68.