复合微生物菌剂对鲫鱼生长及消化功能的影响
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摘要
采用平板培养和微生物全自动菌落计数仪对1种市售水产养殖复合微生物菌剂进行菌群类别和数量检测,并对该复合微生物菌剂对于鲫鱼生长的促进作用进行了分析。结果表明,该种水产养殖复合微生物菌剂主要由放线菌、光合细菌、芽孢杆菌、乳酸菌、酵母菌、硝化细菌六大类菌群组成。该复合微生物制剂投喂鲫鱼,40 d后测定鲫鱼的生长指标及肠道淀粉酶和脂肪酶活力,结果显示,实验组比对照组体质量增长14.28%(P<0.01),体长增长10.3%(P<0.01),肝脏和肠道淀粉酶活力分别提高了290.32%,258.2%(P<0.01),肝脏和肠道脂肪酶活力分别提高了18.4%和28.3%(P<0.01)。说明该复合微生物菌剂能提高鲫鱼的消化能力,从而促进鲫鱼的生长能力。
The microbial community type and quantity of a commercial complex microbial community were determined by plate culture and microbe automatic colony counting instrument. The results showed that the complex microbial community was composed of six microbial communities: actinomycetes, photosynthetic bacteria, bacillus, lactobacillus, yeast, nitrifying bacteria and bacillus bacteria. The complex microbial community was fed to crucian carp 40 days, then the weight and length of crucian carp, as well as the enzymatic activity of intestinal amylase and lipase were measured. The results showed that, compared with the control group, the weight of experimental group increased 14.28%(P<0.01), body length increased 10.3%(P<0.01), liver and intestinal amylase activity respectively increased 290.32%, 258.2%(P<0.01), respectively, liver and intestinal lipase activity increased 18.4% and 18.4% respectively(P<0.01). The complex microbial community could improve the digestive ability of crucian carp and thus promote the growth ability of crucian carp.
The microbial community type and quantity of a commercial complex microbial community were determined by plate culture and microbe automatic colony counting instrument. The results showed that the complex microbial community was composed of six microbial communities: actinomycetes, photosynthetic bacteria, bacillus, lactobacillus, yeast, nitrifying bacteria and bacillus bacteria. The complex microbial community was fed to crucian carp 40 days, then the weight and length of crucian carp, as well as the enzymatic activity of intestinal amylase and lipase were measured. The results showed that, compared with the control group, the weight of experimental group increased 14.28%(P<0.01), body length increased 10.3%(P<0.01), liver and intestinal amylase activity respectively increased 290.32%, 258.2%(P<0.01), respectively, liver and intestinal lipase activity increased 18.4% and 18.4% respectively(P<0.01). The complex microbial community could improve the digestive ability of crucian carp and thus promote the growth ability of crucian carp.
引文
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[2]张旭,王京树,杨元金,等.微生物制剂在水产养殖中的应用[J].畜牧与饲料科学,2011,32(2):101-102.
[3]韦成相,雷奇,张福蓉,等.水产养殖复合微生物制剂中菌群数量分析[J].天津农业科学,2016,22(5):41-45.
[4]谢小平,丁华林,王春林.复合型微生物制剂在水产养殖上的应用[J].江西水产科技,2014(3):47-48.
[5]张玲华,田兴山,邝哲师,等.复合微生物制剂在改善水产养殖微生态环境中的应用[J].广东饲料,2004,13(1):22.
[6]倪寿文.草鱼、鲤、鲢、鳙和尼罗非鲫淀粉酶的比较研究[J].大连水产学院学报,1992,7(1):24-31.
[7]陈红平.浅谈对食品微生物中霉菌和酵母菌计数方法的认识[J].计量与测试技术,2006,33(11):44-45.
[8]律泽,胡筱敏,安婧,等.佳乐麝香和镉复合污染对土壤中放线菌数量的影响[J].生态学杂志,2014,33(6):1501-1507.
[9]韩新锋,刘书亮,赵海伊.发酵肉制品中乳酸菌计数培养基的优化[J].中国酿造,2012,31(6):153-156.
[10]陈捷音.水中亚硝化细菌和硝化细菌检测方法的探讨[J].环境监测管理与技术,2007,19(3):49-51.
[11]中山大学生物系生化微生物学教研室.生化技术导论[M].北京:人民教育出版社,1979:52-88.
[12]王梦亮,郭小青,梁生康,等.光合细菌(PSB)对鲤鱼肠道菌群及肠消化功能的影响[J].中国微生态学杂志,1999,11(3):146-147.
[13]吴婷婷,朱晓鸣.鳜鱼、青鱼、草鱼、鲤、鲫鲢消化酶活性的研究[J].中国水产科学,1994,1(2):11-16.
[14]沈文英,寿建昕.温度对银鲫肠道消化酶活性的影响[J].浙江农业学报,2003(1):35-37.
[15]赵万鹏.草鱼摄食后肝胰脏组织淀粉酶活性的变化[J].水产科学,2002,21(6):13-15.