镉暴露诱导黄颡鱼鳃的组织学损伤、氧化应激和免疫反应
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摘要
为研究水体镉(Cd)暴露对黄颡鱼(Pelteobagrus fulvidraco)鳃的组织学结构、抗氧化状态及免疫相关基因表达的影响,将黄颡鱼分别暴露于0(对照组)、50和200μg/L Cd水体中8周后,取鳃进行分析。结果显示:鳃中Cd含量随着水体Cd浓度的升高而上升;组织学分析发现,Cd暴露组鳃出现了动脉瘤、细胞增生、鳃小片弯曲以及细胞脱落等病理变化。同时,Cd暴露导致鳃中谷胱甘肽-S-转移酶(GST)、谷胱甘肽过氧化物酶(GPx)和抗羟自由基(AHR)的活性及过氧化氢(H_2O_2)的含量升高,而过氧化氢酶(CAT)的活性及还原型谷胱甘肽(GSH)的含量下降,但超氧化物歧化酶(SOD)的活性无显著变化。此外,Cd暴露上调了肿瘤坏死因子-α(tnf-α)、主要组织相容性复合体(mhc)、白细胞介素-10(il-10)、转化生长因子-β(tgf-β)和补体因子C3(c3)的表达,而下调了白细胞介素-1β(il-1β)、白细胞介素-8(il-8)及溶菌酶(lys)的转录水平。研究表明,Cd暴露导致黄颡鱼鳃中Cd富集,进而诱导鳃的组织学损伤、氧化应激和免疫反应。
To investigate the effect of different Cd(0(control),50,and 200 μg/L Cd2+) on Yellow catfish(Pelteobagrus fulvidraco),fish were exposed for 8 weeks before analyzing the histological structure,antioxidant status and immune-related gene expression in gills.The results showed that Cd2+ exposure induced significant Cd accumulation and histological changes in gill,such as aneurism,hyperplasia,curling of lamella and cell desquamation.Meanwhile,Cd2+increased the activity of glutathione-S-transferase(GST),glutathione peroxidase(GPx) and anti-hydroxyl radical(AHR) as well as the content of hydrogen peroxide(H_2O_2),and it decreased catalase(CAT) activity and glutathione(GSH) content.However,Cd2+ had no significant effect on superoxide dismutase(SOD) activity.In addition,Cd exposure up-regulated tumor necrosis factor-a(tnf-a),major histocompatibility complex(mhc),interleukin-10(il-10),transforming growth factor-β(tgf-β) and complement factor C3(c3) expressions,but down-regulated interleukin-1β(il-1β),interleukin-8(il-8) and lysozyme(lys) transcription levels.These results showed that Cd exposure resulted in the accumulation of Cd,and induced histological damage,oxidative stress and immune response of gill in yellow catfish.
To investigate the effect of different Cd(0(control),50,and 200 μg/L Cd2+) on Yellow catfish(Pelteobagrus fulvidraco),fish were exposed for 8 weeks before analyzing the histological structure,antioxidant status and immune-related gene expression in gills.The results showed that Cd2+ exposure induced significant Cd accumulation and histological changes in gill,such as aneurism,hyperplasia,curling of lamella and cell desquamation.Meanwhile,Cd2+increased the activity of glutathione-S-transferase(GST),glutathione peroxidase(GPx) and anti-hydroxyl radical(AHR) as well as the content of hydrogen peroxide(H_2O_2),and it decreased catalase(CAT) activity and glutathione(GSH) content.However,Cd2+ had no significant effect on superoxide dismutase(SOD) activity.In addition,Cd exposure up-regulated tumor necrosis factor-a(tnf-a),major histocompatibility complex(mhc),interleukin-10(il-10),transforming growth factor-β(tgf-β) and complement factor C3(c3) expressions,but down-regulated interleukin-1β(il-1β),interleukin-8(il-8) and lysozyme(lys) transcription levels.These results showed that Cd exposure resulted in the accumulation of Cd,and induced histological damage,oxidative stress and immune response of gill in yellow catfish.
引文
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[36]Thuvander A.Cadmium exposure of rainbow trout,Salmo gairdneri Richardson:effects on immune functions[J].Journal of Fish Biology,1989,35(4):521-529
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[38]Guardiola F A,Cuesta A,Meseguer J,et al.Accumulation,histopathology and immunotoxicological effects of waterborne cadmium on gilthead seabream(Sparus aurata)[J].Fish&Shellfish Immunology,2013,35(3):792-800
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[40]Zhao J,Liu Y,Jiang J,et al.Effects of dietary isoleucine on the immune response,antioxidant status and gene expression in the head kidney of juvenile Jian carp(Cyprinus carpio var.Jian)[J].Fish&Shellfish Immunology,2013,35(2):572-580
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[42]Roca F J,Mulero I,López-Mu?oz A,et al.Evolution of the inflammatory response in vertebrates:fish TNF-alpha is a powerful activator of endothelial cells but hardly activates phagocytes[J].Journal of Immunology,2008,181(7):5071-5081
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[2]Das S,Mukherjee D.Effect of cadmium chloride on secretion of 17β-estradiol by the ovarian follicles of common carp,Cyprinus carpio[J].General and Comparative Endocrinology,2013,181(2):107-114
[3]Jin Y X,Liu Z Z,Liu F,et al.Embryonic exposure to cadmium(Ⅱ)and chromium(Ⅵ)induce behavioral alterations,oxidative stress and immunotoxicity in zebrafish(Danio rerio)[J].Neurotoxicology and Teratology,2015,48:9-17
[4]He L L,Chen Z H.Monitoring of heavy metal pollution in water environment of wuhan[J].Environmental Science&Technology,2007,30:40-41
[5]Ning L,Yang L Y,Dai J R,et al.Heavy metal pollution in surface water of linglong gold mining area,China[J].Procedia Environmental Sciences,2011,10(1):914-917
[6]Bai S J,Xu Z R.Effects of cadmium on mitochondrial structure and energy metabolism of yellow catfish[J].Chinese Journal of Applied Ecology,2006,17(7):1213-1217[柏世军,许梓荣.镉对黄颡鱼鳃线粒体结构和能量代谢的影响.应用生态学报,2006,17(7):1213-1217]
[7]Liu T T,Dong X X,Miao M S,et al.Sensitivity of embryo and yolk-sac fry of japanese medaka(Oryzias latipes)to cadmium[J].Asian Journal of Ecotoxicology,2012,7(2):177-181[刘腾腾,董晓晓,苗明升,等.日本青鳉(Oryzias latipes)胚胎和卵黄期仔鱼对镉的敏感性.生态毒理学报,2012,7(2):177-181]
[8]Shi H Q,Zhang L J,Yuan X Y,et al.Toxic effects of cadmium chloride exposure on neurobehavior of zebrafish larvae[J].Asian Journal of Ecotoxicology,2013,8(3):374-380[史慧勤,张利军,苑晓燕,等.氯化镉暴露对斑马鱼幼鱼神经行为毒性作用.生态毒理学报,2013,8(3):374-380]
[9]Rubens M M,Edith F.Effects of the organophosphorous methyl parathion on the branchial epithelium of a freshwater fish Metynnis roosevelti[J].Brazilian Archives of Biology and Technology,2003,46(3):361-372
[10]Perry S F,Shahsavarani A,Georgalis T,et al.Channels,pumps,and exchangers in the gill and kidney of freshwater fishes:their role in ionic and acid-base regulation[J].Journal of Experimental Zoology Part A Ecological Genetics and Physiology,2003,300(1):53-62
[11]Heuvel M R V D,Power M,Richards J,et al.Disease and gill lesions in yellow perch(Perca flavescens)exposed to oil sands mining-associated waters[J].Ecotoxicology and Environmental Safety,2000,46(3):334-341
[12]Bernet D,Schmidt H,Meier W,et al.Histopathology in fish:proposal for a protocol to assess aquatic pollution[J].Journal of Fish Diseases,1999,22(1):25-34
[13]Chen Q L,Gong Y,Luo Z,et al.Differential effect of waterborne cadmium exposure on lipid metabolism in liver and muscle of yellow catfish Pelteobagrus fulvidraco[J].Aquatic Toxicology,2013,142:380-386
[14]Annabi A,Messaoudi I,Kerkeni A,et al.Cadmium accumulation and histological lesion in mosquitofish(Gambusia affinis)tissues following acute and chronic exposure[J].International Journal of Environmental Research,2011,5(3):745-756
[15]Luo Z,Li X,Bai H,et al.Effects of dietary fatty acid composition on muscle composition and hepatic fatty acid profile in juvenile Synechogobius hasta[J].Journal of Applied Ichthyology,2008,24(1):116-119
[16]Sobha K,Poornima A,Harini P,et al.A study on biochemical changes in the fresh water fish,Catla catla(Hamilton)exposed to the heavy metal toxicant cadmium chloride[J].Kathmandu University Journal of Science Engineering&Technology,2007,3(2):1-11
[17]Kim S G,Jee J H,Kang J C.Cadmium accumulation and elimination in tissues of juvenile olive flounder,Paralichthys olivaceus after sub-chronic cadmium exposure[J].Environmental Pollution,2004,127(1):117-123
[18]Liu C F,Tao S,Long A M.Accumulations of lead and cadmium in goldfish Carassius auratus[J].Acta Hydrobiologica Sinica,2001,25(4):344-349[刘长发,陶澍,龙爱民.金鱼对铅和镉的吸收蓄积.水生生物学报,2001,25(4):344-349]
[19]Abdel-Moneim A M,Al-Kahtani M A,Elmenshawy OM.Histopathological biomarkers in gills and liver of Oreochromis niloticus from polluted wetland environments,Saudi Arabia[J].Chemosphere,2012,88(8):1028-1035
[20]Liu X J,Luo Z,Li C H,et al.Antioxidant responses,hepatic intermediary metabolism,histology and ultrastructure in Synechogobius hasta exposed to waterborne cadmium[J].Ecotoxicology and Environmental Safety,2011,74(5):1156-1163
[21]Thophon S,Kruatrachue M,Upatham E S,et al.Histopathological alterations of white seabass,Lates calcarifer,in acute and subchronic cadmium exposure[J].Environmental Pollution,2003,121(3):307-320
[22]Mallatt J.Fish gill structural changes induced by toxicants and other irritants:a statistical review[J].Canadian Journal of Fisheries and Aquatic Sciences,1985,42(4):630-648
[23]Camargo M M P,Martinez C B R.Histopathology of gills,kidney and liver of a Neotropical fish caged in an urban stream[J].Neotropical Ichthyology,2007,5(3):327-336
[24]Cao L A,Huang W,Liu J H,et al.Accumulation and oxidative stress biomarkers in Japanese flounder larvae and juveniles under chronic cadmium exposure[J].Comparative Biochemistry and Physiology Part C Toxicology&Pharmacology,2010,151(3):386-392
[25]López E,Arce C,Oset-Gasque M J,et al.Cadmium induces reactive oxygen species generation and lipid peroxidation in cortical neurons in culture[J].Free Radical Biology and Medicine,2006,40(6):940-951
[26]Hallare A,Nagel K,K?hler H R,et al.Comparative embryotoxicity and proteotoxicity of three carrier solvents to zebrafish(Danio rerio)embryos[J].Ecotoxicology and Environmental Safety,2006,63(3):378-388
[27]Monteiro D A,Rantin F T,Kalinin A L.Inorganic mercury exposure:toxicological effects,oxidative stress biomarkers and bioaccumulation in the tropical freshwater fish matrinx?,Brycon amazonicus(Spix and Agassiz,1829)[J].Ecotoxicology,2010,19(1):105-123
[28]Mccord J M,Fridovich I.Superoxide dismutase an enzymic function for erythrocuprein(hemocuprein)[J].Journal of Biological Chemistry,1969,244(22):6049-6055
[29]Thomas J P,Maiorino M,Ursini F,et al.Protective action of phospholipid hydroperoxide glutathione peroxidase against membrane-damaging lipid peroxidation.In situ reduction of phospholipid and cholesterol hydroperoxides[J].Journal of Biological Chemistry,1990,265(1):454-461
[30]Pandey S,Parvez S,Sayeed I,et al.Biomarkers of oxidative stress:a comparative study of river Yamuna fish Wallago attu(Bl.&Schn.)[J].Science of the Total Environment,2003,309(1-3):105-115
[31]Basha P S,Rani A U.Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus(Tilapia)[J].Ecotoxicology and Environmental Safety,2003,56(2):218-221
[32]Farombi E O,Adelowo O A,Ajimoko Y R.Biomarkers of oxidative stress and heavy metal levels as indicators of environmental pollution in african cat fish(Clarias gariepinus)from Nigeria Ogun River[J].International Journal of Environmental Research and Public Health,2007,4(2):158-165
[33]Mohamed S,Kheireddine O,Wyllia H M,et al.Proportioning of biomarkers(GSH,GST,AChE,Catalase)indicator of pollution at Gambusia affinis(Teleostei Fish)exposed to cadmium[J].Environmental Research Journal,2008,2(4):177-181
[34]Tanzadehpanah H,Asoodeh A,Mahaki H,et al.Bioactive and ACE binding properties of three synthetic peptides assessed by various spectroscopy techniques[J].Process Biochemistry,2016,51(12):2067-2075
[35]Magnadóttir B.Innate immunity of fish(overview)[J].Fish&Shellfish Immunology,2006,20(2):137-151
[36]Thuvander A.Cadmium exposure of rainbow trout,Salmo gairdneri Richardson:effects on immune functions[J].Journal of Fish Biology,1989,35(4):521-529
[37]Wang H J,Lin S X,Zhou L F,et al.The effect of exposure to five kinds of heavy metals on respiratory movement of zebra fish(Brachydanio rerio)[J].Journal of Agro-Environment Science,2010,29(9):1675-1680[汪红军,李嗣新,周连凤,等.5种重金属暴露对斑马鱼呼吸运动的影响.农业环境科学学报,2010,29(9):1675-1680]
[38]Guardiola F A,Cuesta A,Meseguer J,et al.Accumulation,histopathology and immunotoxicological effects of waterborne cadmium on gilthead seabream(Sparus aurata)[J].Fish&Shellfish Immunology,2013,35(3):792-800
[39]Alkharashi N A O,Periasamy V S,Athinarayanan J,et al.Cadmium triggers mitochondrial oxidative stress in human peripheral blood lymphocytes and monocytes:Analysis using in vitro and system toxicology approaches[J].Journal of Trace Elements in Medicine and Biology,2017,42:117-128
[40]Zhao J,Liu Y,Jiang J,et al.Effects of dietary isoleucine on the immune response,antioxidant status and gene expression in the head kidney of juvenile Jian carp(Cyprinus carpio var.Jian)[J].Fish&Shellfish Immunology,2013,35(2):572-580
[41]Secombes C J.Cytokines and innate immunity of fish[J].Developmental&Comparative Immunology,2001,25(8-9):713-723
[42]Roca F J,Mulero I,López-Mu?oz A,et al.Evolution of the inflammatory response in vertebrates:fish TNF-alpha is a powerful activator of endothelial cells but hardly activates phagocytes[J].Journal of Immunology,2008,181(7):5071-5081
[43]Rammensee H,Bachmann J,Emmerich N P,et al.SY-FPEITHI:database for MHC ligands and peptide motifs[J].Immunogenetics,1999,50(4):213-219
[44]Neumann H,Schmidt H,CavaliéA,et al.Major histocompatibility complex(MHC)class I gene expression in single neurons of the central nervous system:differential regulation by interferon(IFN)-γand tumor necrosis factor(TNF)-α[J].Journal of Experimental Medicine,1997,185(2):305-316
[45]Dinarello C A.Immunological and inflammatory functions of the interleukin-1 family[J].Annual Review of Immunology,2009,27(27):519-550
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