~(15)N自然丰度法分析甘蔗及近缘属野生种固氮效率
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摘要
利用~(15)N同位素自然丰度法,在连续两年不施氮肥条件下,对甘蔗及近缘属野生种(材料)的固氮效率进行对比分析,同时筛选出适宜本试验研究的参比植物。试验结果表明,河八王及其后代材料表现出明显的高固氮能力,最高固氮率达到36.13%,最低的为甘蔗栽培种ROC22,固氮率仅为5.72%。银胶菊、胜红蓟和狗尾草与河八王1-1号的δ~(15)N值之差为3.13~3.50,是分析甘蔗及近缘属野生种固氮效率较理想的参比植物。
The nitrogen fixation efficiency of sugarcane and its related genera were analyzed by15 N natural abundance method for two consecutive years without nitrogen fertilizer application. The results showed that the Narenga porphyrocoma(Hance) Bor. and it's progeny had relatively higher nitrogen fixation rate(NFR) compared with other sugarcane and related genera, the highest NFR reached 36.13%, and the lowest was commercial varieties ROC22 of 5.72%. The differences of δ15N between the Narenga porphyrocoma(Hance) Bor and the reference plants(guayule, tropic ageratum herb and green bristlegrass) ranged from 3.13 to 3.50, which were ideal reference plants to calculate nitrogen fixation efficiency in sugarcane and its related genera.
The nitrogen fixation efficiency of sugarcane and its related genera were analyzed by15 N natural abundance method for two consecutive years without nitrogen fertilizer application. The results showed that the Narenga porphyrocoma(Hance) Bor. and it's progeny had relatively higher nitrogen fixation rate(NFR) compared with other sugarcane and related genera, the highest NFR reached 36.13%, and the lowest was commercial varieties ROC22 of 5.72%. The differences of δ15N between the Narenga porphyrocoma(Hance) Bor and the reference plants(guayule, tropic ageratum herb and green bristlegrass) ranged from 3.13 to 3.50, which were ideal reference plants to calculate nitrogen fixation efficiency in sugarcane and its related genera.
引文
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[2]Ju X,Xing G,Chen X,Zhang S,Zhang L,Liu X,Cui Z,Yin B,Christie P,Zhu Z,Zhang F.Reducing environmental risk by improving N management in intensive Chinese agricultural systems[J].Proceedings of the National Academy of Sciences of the USA2009,106(9):3041-3046.
[3]Guo J,Liu X,Zhang Y,Shen J,Han W,Zhang W,Chrisie P,Goulding K,Vitousek P,Zhang F.Significant acidification in major Chinese croplands[J].Science 2010,327(5968):1008-1010.
[4]Woolf D,Amonette J E,Street-Perrott F A,Lehmann J,Joseph S.Sustainable biochar to mitigate global climate change[J].Nature Communications,2010,1(3):118-124.
[5]Thorburn P,Biggs J,Webster A,Biggs I.An improved way to determine nitrogen fertilizer requirements of sugarcane crops to meet global environmental challenges[J].Plant and Soil,2011,339(1):51-67.
[6]Boddey R M,Polidoro J C,Resende A S,Alves B J R,Urquiaga S.Use of the15N natural abundance technique for the quantification of the contribution of N2fixation to sugar cane and other grasses[J].Australian Joumal of Plant Physiology,2000,28(9):889-895.
[7]Fuentes-Ramirez LE,Bustillos-Cristales R,Tapia-Hernandez A,Jimenez-Salgado T,Wang ET,Martinez-Romero E,CaballeroMellado J.Novel nitrogen-fixing acetic acid bacteria,Gluconacetobacter johannae sp.nov.and Gluconacetobacter azotocaptans sp.nov.,associated with coffee plants[J].International Journal of Systematic and Evolutionary Microbiology,2001,51(4):1305-1314.
[8]Ando S,Meunchang S,Thippayarugs S,Prasertsak P,.Matsumoto N,Yoneyama T.Evaluation of sustainability of sugarcane production in Thailand based on nitrogen fixation,efficiency of nitrogen fertilizer and flow of organic matters[J].JIRCAS Working Report,2002,30:61-64.
[9]Luo T,Ou-Yang X Q O-Y,Yang L T,Li Y R,Song X P,Zhang G M,Gao Y J,Duan W X,An Q L.Raoultella sp.strain L03 fixes N2in association with micropropagated sugarcane plants[J].Journal of Basic Microbiology,2016,56(8):934-940.
[10]Lin Li,Zhengyi L,Chunjing H,Xincheng Z,Siping C,Litao Y,Yangrui L,and Qianli A.Plant growth-promoting nitrogen-fixing enterobacteria are in association with sugarcane plants growing in Guangxi,China[J].Microbes&Environ.,2012,27(4):391-398.
[11]何道文,孙辉,黄雪菊.利用15N自然丰度法研究固氮植物生物固氮量[J].干旱地区农业研究,2004,21(1):132-137.
[12]Amarger N,Mariotti A,Mariotti F,Curr J C,Bourguignon C,Lagacherie B.Estimate of symbiotically fixed nitrogen in field grown soybeans using variations in15N Natural abundance[J].Plant and soil,1979,52(2):269-280.
[13]Rennie D A,Paul E A,Johns L E.Natural nitrogen-15 abundance of soil and plant samples[J].Canadian Journal of Soil Science,1976,56(1):43-50.