法半夏炮制工艺的Box-Behnken响应面法优化
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摘要
目的研究法半夏炮制工艺的关键影响因素,优选法半夏炮制工艺。方法在单因素试验的基础上,以水溶性浸出物、鸟苷、甘草酸二铵含量的总评归一值(OD)为指标,对浸泡时间、干燥温度及干燥时间这3个因素进行响应面试验研究,从而得出最佳炮制工艺。结果优选最佳工艺条件为生半夏与甘草提取液和生石灰浸泡3天,于72℃干燥24h。该炮制工艺条件所测的实际值与理论值基本一致,偏差较小。结论通过响应面法优化法半夏炮制工艺,简便易行,结果可靠,能为法半夏质量控制和规模化生产提供科学依据和新颖思路。
Objective To study the key factors of processing technology of Rhizome Pinelliae and to optimize it by Box-Behnken design response surface methodology. Methods On the basis of single factor screening,Box-Behnken experimental design was employed,the overall desirability,which was based on the content of water soluble extract,guanosine,and diammonium glycyrrhizinate,were used as evaluation index. The soaking temperature,drying temperature,drying duration were used as independent variables. Results The optimal processing conditions were as follows: crude Rhizome Pinelliae was soaked in liquid extraction of Radix Glycyrrhizae,with calcium oxide,for 3 days,and dried at the temperature of 72℃ for 24 hours. The actual values determined by the response surface methodology are basically consistent with the theoretical values, with little deviation.Conclusion The optimized processing technology is easy to operate and the results are accurate and reliable,which could provide scientific basis and innovative idea for quality control and large-scale production of Rhizome Pinelliae.
Objective To study the key factors of processing technology of Rhizome Pinelliae and to optimize it by Box-Behnken design response surface methodology. Methods On the basis of single factor screening,Box-Behnken experimental design was employed,the overall desirability,which was based on the content of water soluble extract,guanosine,and diammonium glycyrrhizinate,were used as evaluation index. The soaking temperature,drying temperature,drying duration were used as independent variables. Results The optimal processing conditions were as follows: crude Rhizome Pinelliae was soaked in liquid extraction of Radix Glycyrrhizae,with calcium oxide,for 3 days,and dried at the temperature of 72℃ for 24 hours. The actual values determined by the response surface methodology are basically consistent with the theoretical values, with little deviation.Conclusion The optimized processing technology is easy to operate and the results are accurate and reliable,which could provide scientific basis and innovative idea for quality control and large-scale production of Rhizome Pinelliae.
引文
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[2]国家药典委员会.中国药典,一部[S].北京:中国医药科技出版社,2010.
[3]蒋万浪.Box-Behnken设计优化熟三七皂苷类成分提取工艺[J].中药材,2016,39(8):1825.
[4]李化,柯华香,李发洁,等.Box-Behnken响应面法优选五味子多糖双水相提取工艺[J].中药材,2016,39(3):593.
[5]魏运娇,赵熠,宋红萍,等.正交设计法研究法半夏的炮制工艺[J].中国医院药学杂志,2013,33(2):134.
[6]张琳,吴皓,朱涛,等.多指标正交试验优化清半夏炮制工艺[J].中成药,2008,30(5):704.
[7]梁君,刘小鸣,张振凌,等.姜半夏产地加工炮制一体化方法及工艺研究[J].中草药,2015,46(9):1302.
[8]陈敬,温庆果,刘韶,等.正交设计与响应面法优化壳聚糖对莲子心提取液除杂工艺对比研究[J].中草药,2012,43(11):2183.
[9]黄文青,高明,刘松,等.响应面法优化蒸制姜半夏的炮制工艺[J].中药材,2015,38(7):1403.
[10]吴皓,文红梅,郭戎,等.半夏姜制对鸟苷的影响[J].中国中药杂志,1998,23(11):661.
[11]杨锡,张伯崇.法半夏炮制过程中甘草酸的动态分析及炮制条件的选择[J].中药材,1992,15(7):26.
[12]杨锡,张伯崇,常克俭,等.法半夏中甘草酸的检测研究[J].中成药,1991,13(8):17.
[13]魏淑红,彭正松.半夏类药材研究概况[J].中药材,2003,26(11):828.
[14]严茂伟,万军,楚亮,等.半夏干燥过程中褐变机制的研究[J].中草药,2011,42(5):877.
[15]单鸣秋,钱岩,于生,等.基于响应面法的天麻产地加工炮制一体化工艺研究[J].中草药,2016,47(3):420.