反相纸层析法分离油菜籽中脂肪酸的经验总结
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摘要
在利用反相纸层析法分离油菜籽中主要不饱和脂肪酸的实验教学中,由于受到多种因素的影响,脂肪酸层析图谱经常出现各种问题。经过大量实验摸索,不断优化实验条件,提高了反相纸层析法分离主要不饱和脂肪酸的实验教学效果。对脂肪酸层析进行经验总结,同时对常见问题进行解析并提出相应的解决方案,指导脂肪酸层析实验教学。
In the experimental teaching in the separation of main unsaturated fatty acids from rapeseed by reversed-phase paper chromatography, the fatty acid chromatogram often appears various problems due to a variety of factors. Through a lot of experiments and continuous optimization of experimental conditions, the experimental teaching effect of the separation of main unsaturated fatty acids by the reversed-phase paper chromatography was improved. The experience of fatty acid chromatography was summarized. Meanwhile, the common problems were analyzed and the corresponding solutions were putted forward. These can guide the experimental teaching of fatty acid chromatography.
In the experimental teaching in the separation of main unsaturated fatty acids from rapeseed by reversed-phase paper chromatography, the fatty acid chromatogram often appears various problems due to a variety of factors. Through a lot of experiments and continuous optimization of experimental conditions, the experimental teaching effect of the separation of main unsaturated fatty acids by the reversed-phase paper chromatography was improved. The experience of fatty acid chromatography was summarized. Meanwhile, the common problems were analyzed and the corresponding solutions were putted forward. These can guide the experimental teaching of fatty acid chromatography.
引文
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[15]教育部办公厅关于2017-2020年开展示范性虚拟仿真实验教学项目建设的通知[EB/OL]. (2017-07-13)[2018-01-28]. http://www.moe.edu.cn /srcsite/A08/s7945/ s7946/201707/ t201707 21_309819.html
[2]周文斌,吕春明,张宁,等. 脂肪酸检测方法的研究进展[J]. 中国现代应用药学,2014,32(2):247-251.
[3]李培武,谢立华,魏立芳,等. 反式脂肪酸检测方法的研究与应用[J]. 中国油粮作物学报,2009,31(3):374-379.
[4]赵美娜,段佳林,牟菲,等.液质联用技术检测脂肪酸的研究进展[J]. 中国新药杂志,2017,26(19):2278-2284.
[5]罗伟强. 气相色谱法测定葵花籽油的脂肪酸[J].食品工业科技,2003,24(6):79-80.
[6]郭华,周建平,罗军武,等.茶籽油的脂肪酸组成测定[J].中国油脂,2008,33(7):71-73.
[7]马彦玲,孙莲,张慧,等.气相色谱法测定芜菁子油脂中6 种脂肪酸的含量[J].新疆医科大学学报,2010,33(11):1296-1298.
[8]吴建国,石春海,张海珍.构建整粒油菜籽脂肪酸成分近红外反射光谱分析模型的研究[J].光谱学与光谱分析,2006,26(2):259-262.
[9]杨翠玲,陈文杰,张文学,等. 近红外光谱和纸层析法分析单粒油菜籽脂肪酸的研究[J]. 西北农业学报,2005,14(6):72-77.
[10]魏小豹,韦莹莹,薛璟琪,等. 高温加热对牡丹籽油脂肪酸和理化性质的影响[J].食品科学, 2018, 39(3):15-20.
[11]郝苗,戴学东,姜波,等. 气相色谱-质谱法测定三种茶叶中的脂肪酸[J].天津农业科学,2016,22(10):15-17.
[12]尹亚军,章丽琳,张喻. 油菜籽成熟过程中主要脂肪酸变化的研究[J]. 中国粮油学报,2016,31(7):82-88.
[13]王学奎,黄见良.植物生理生化实验原理和技术 [M]. 3版.北京:高等教育出版社,2015:263-264.
[14]闵华秀. 纸层析法测定油菜籽芥酸含量的几个问题探讨[J]. 贵州农业科学,1987(5):38-40.
[15]教育部办公厅关于2017-2020年开展示范性虚拟仿真实验教学项目建设的通知[EB/OL]. (2017-07-13)[2018-01-28]. http://www.moe.edu.cn /srcsite/A08/s7945/ s7946/201707/ t201707 21_309819.html