高效液相色谱法测定制麦中阿魏酸含量
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摘要
建立了一种测定制麦中阿魏酸含量的高效液相色谱法。选用色谱柱为Agilent Eclipse XDB-C18(4.6mm×250mm,5μm),流动相为甲醇-冰醋酸体系(60∶40,pH3.0),流速1.0ml/min,柱温35℃,紫外检测器波长320nm,进样量为20μl,对制麦中阿魏酸含量进行测定分析。结果表明:该方法条件下阿魏酸浓度在0.25~200μg/g时,线性关系良好(R2=0.999 9)。制麦过程中阿魏酸含量显著升高(P<0.05),麦芽阶段含量最高。所建方法分析速度快、操作简便、结果的精密度与重现性好,可作为制麦中阿魏酸含量的测定方法。
The study was to establish a HPLC method to determine the content of ferulic acid in malt.The chromatographic conditions were as follows:the chromatographic column was Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm),methanol-glacial acetic acid(60∶40,pH 3.0)as mobile phase with a flow rate of 1.0 ml/min,column temperature was 35℃,the detection wavelength was 320 nm andthe sample amount was 20μl.The method had good linear relationship when the quality ratio of ferulic acid was 0.25~200μg/g(R2=0.999 9).The content of ferulic acid during malting was significantly increased(P<0.05),which was the highest in malt.The method is more simple and faster,which could be used to determine the content of ferulic acid during malting for wheat.
The study was to establish a HPLC method to determine the content of ferulic acid in malt.The chromatographic conditions were as follows:the chromatographic column was Agilent Eclipse XDB-C18(4.6 mm×250 mm,5μm),methanol-glacial acetic acid(60∶40,pH 3.0)as mobile phase with a flow rate of 1.0 ml/min,column temperature was 35℃,the detection wavelength was 320 nm andthe sample amount was 20μl.The method had good linear relationship when the quality ratio of ferulic acid was 0.25~200μg/g(R2=0.999 9).The content of ferulic acid during malting was significantly increased(P<0.05),which was the highest in malt.The method is more simple and faster,which could be used to determine the content of ferulic acid during malting for wheat.
引文
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[10]李喜凤,邱天宝,胡亚楠,等.高效毛细管电泳法测定蒲公英中阿魏酸的含量[J].中国实验方剂学杂志,2010,16(16):27-29.
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[12]李全斌,何开勇,吴建萍.阿魏酸含量测定方法研究进展[J].中医药导报,2011,17(3):117-118.
[13]魏岚,颜振敏.高效液相色谱法测定小麦中阿魏酸的含量[J].安徽农业科学,2012,40(28):13 693-13 728.
[14]陈锦,胡茂丰,文欣,等.HPLC测定不同厂家发酵型茶叶中阿魏酸含量[J].中国酿造,2015,34(4):161-164.
[15]中华人民共和国卫生部.GB/T 23196-2008蜂胶中阿魏酸含量的测定方法液相色谱-紫外检测法[S].北京:中国标准出版社,2008.
[16]廖律,周明达,肖劲,等.米糠中阿魏酸的提取及高效液相色谱法测定[J].食品工业科技,2007,28(2):217-229.
[17]王瑞英.HPLC法测定香味康胶囊中阿魏酸的含量[J].中国当代医药,2010,17(25):46-48.
[18]曹斌斌.泌乳奶牛瘤胃微生物饲料细胞壁阿魏酸和香豆酸消化代谢研究[D].北京:中国农业大学,2015.
[19]涂禾,梁栗,侯世祥,等.高效液相色谱法测定疲王按摩乳中阿魏酸的含量[J].华西医学,2014,29(9):1 698-1 699.
[20]陈锡龙.高效液相色谱柱的管理、维护、再生及修复技术[J].贵州畜牧兽医,2016,40(5):23-24.
[21]LV K,DING M Y,LI H X,et al.Determination of ferulic acid in chuanxiong and in animal serum and cerebrospinal fluid by reversed-phase High Performance Liquid Chromatography[J].Chinses J Chromato,2000,18(6):518-520.
[22]郝影霞,王逢春,王焱.高效液相色谱法测定活血清脑丸中阿魏酸的含量[J].解放军医药杂志,2012,24(7):29-30.
[2]王丹.小麦与麦芽中阿魏酸及其阿魏酸酯酶的研究[D].泰安:山东农业大学,2011:41.
[3]张晓宇,李建新,王育红,等.小麦芽在食品工业中的应用研究进展[J]粮食与饲料工业,2015(6):33-35.
[4]宋凯,单安山,燕昌江,等.阿魏酸的生理作用及其在饲料中的应用前景[J].饲料博览,2004(9):44-45.
[5]赵同峰,邓华聪.阿魏酸钠对糖尿病大鼠肾脏抗氧化酶的影响[J].重庆医科大学学报,2002,27(4)430-432.
[6]欧仕益,包惠燕.阿魏酸及其衍生物的药理作用研究进展[J].中药材,2001,24(3):220-221.
[7]冯艺戎.TLCS法测定安中养血合剂中阿魏酸的含量[J].安徽医药,2010,14(6):654-655.
[8]李秋怡,干国平,王光忠,等.气相色谱法测定川芎油中阿魏酸的含量[J].时珍国医国药,2007,18(7):1 687-1 688.
[9]王丹,杜金华.比色法快速测定小麦及其麦芽中的阿魏酸[J].食品与发酵工业,2011,37(1):146-151.
[10]李喜凤,邱天宝,胡亚楠,等.高效毛细管电泳法测定蒲公英中阿魏酸的含量[J].中国实验方剂学杂志,2010,16(16):27-29.
[11]FACCIN H,ROBERTA FABRICIO L,CARINE V,et al.Determination of phenolic compounds in extracts of Aamazonian medicinal plants by liquid chromatography-electrospray tandem mass spectrometry[J].Anal Methods,2017,9(7):1 141-1 151.
[12]李全斌,何开勇,吴建萍.阿魏酸含量测定方法研究进展[J].中医药导报,2011,17(3):117-118.
[13]魏岚,颜振敏.高效液相色谱法测定小麦中阿魏酸的含量[J].安徽农业科学,2012,40(28):13 693-13 728.
[14]陈锦,胡茂丰,文欣,等.HPLC测定不同厂家发酵型茶叶中阿魏酸含量[J].中国酿造,2015,34(4):161-164.
[15]中华人民共和国卫生部.GB/T 23196-2008蜂胶中阿魏酸含量的测定方法液相色谱-紫外检测法[S].北京:中国标准出版社,2008.
[16]廖律,周明达,肖劲,等.米糠中阿魏酸的提取及高效液相色谱法测定[J].食品工业科技,2007,28(2):217-229.
[17]王瑞英.HPLC法测定香味康胶囊中阿魏酸的含量[J].中国当代医药,2010,17(25):46-48.
[18]曹斌斌.泌乳奶牛瘤胃微生物饲料细胞壁阿魏酸和香豆酸消化代谢研究[D].北京:中国农业大学,2015.
[19]涂禾,梁栗,侯世祥,等.高效液相色谱法测定疲王按摩乳中阿魏酸的含量[J].华西医学,2014,29(9):1 698-1 699.
[20]陈锡龙.高效液相色谱柱的管理、维护、再生及修复技术[J].贵州畜牧兽医,2016,40(5):23-24.
[21]LV K,DING M Y,LI H X,et al.Determination of ferulic acid in chuanxiong and in animal serum and cerebrospinal fluid by reversed-phase High Performance Liquid Chromatography[J].Chinses J Chromato,2000,18(6):518-520.
[22]郝影霞,王逢春,王焱.高效液相色谱法测定活血清脑丸中阿魏酸的含量[J].解放军医药杂志,2012,24(7):29-30.