高效液相色谱-蒸发光散射法同时测定山银花提取物中5种皂苷的含量
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摘要
目的建立高效液相色谱-蒸发光散射法(high performance liquid chromatography-evaporative light scattering,HPLC-ELSD)同时测定山银花提取物中5种皂苷的含量的分析方法。方法采用Agilent ZORBAX SB-C_(18)(250 mm×4.6 mm, 5μm),以乙腈-0.1%甲酸溶液作为流动相进行梯度洗脱,流速为0.8 mL/min,柱温为30℃,漂移管温度为60℃。结果 5种皂苷(灰毡毛忍冬皂苷乙、灰毡毛忍冬皂苷甲、川续断皂苷乙、灰毡毛忍冬次皂苷乙和灰毡毛忍冬次皂苷甲)在一定范围内线性关系良好(r为0.9989～0.9999),回收率为95.74%～97.02%,相对标准偏差(relative standard deviation, RSD)为0.9%～1.4%(n=6)。结论本法简便、可靠、准确,适用于山银花提取物的质量控制。
Objective To establish a method for simultaneous determination of 5 kinds of saponins in Lonicera hypoglauca extract by high performance liquid chromatography-evaporative light scattering(HPLC-ELSD).Methods Agilent ZORBAX SB-C_(18)(250 mm×4.6 mm, 5 μm) was used, and gradient elution was conducted with acetonitrile-0.1% formic acid as mobile phase for determination. Flow rate was 0.8 mL/min, column temperature was 30 ℃, and drift tube temperature was 60 ℃. Results The 5 kinds of saponins(macranthoidin A, macranthoidin B,dipsacoside B, macranthoside A and macranthoside B) had a good linear correlation in certain range(r were 0.9989-0.9999). The average recoveries were 95.74%-97.02%, and relative standard deviations(RSD) were 0.9%-1.4%(n=6). Conclusion This method is simple, reliable, accurate, which is suitable for the quality control for Lonicera hypoglauca extract.
Objective To establish a method for simultaneous determination of 5 kinds of saponins in Lonicera hypoglauca extract by high performance liquid chromatography-evaporative light scattering(HPLC-ELSD).Methods Agilent ZORBAX SB-C_(18)(250 mm×4.6 mm, 5 μm) was used, and gradient elution was conducted with acetonitrile-0.1% formic acid as mobile phase for determination. Flow rate was 0.8 mL/min, column temperature was 30 ℃, and drift tube temperature was 60 ℃. Results The 5 kinds of saponins(macranthoidin A, macranthoidin B,dipsacoside B, macranthoside A and macranthoside B) had a good linear correlation in certain range(r were 0.9989-0.9999). The average recoveries were 95.74%-97.02%, and relative standard deviations(RSD) were 0.9%-1.4%(n=6). Conclusion This method is simple, reliable, accurate, which is suitable for the quality control for Lonicera hypoglauca extract.
引文
[1]国家药典委员会.中国药典[M].北京:中国医药科技出版社,2015.Chinese Pharmacopoeia Commission.Chinese pharmacopoeia[M].Beijing:China Medical Science Press,2015.
[2]张英锋,张立艳,马子川,等.植物绿原酸的研究进展[J].化学教育,2011,32(4):1-6.Zhang YF,Zhang LY,Ma ZC,et al.Advancement of plant chlorogenic acid[J].J Chem Edu,2011,32(4):1-6.
[3]席利莎,木泰华,孙红男,等.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.Xi LS,Mu TH,Sun HN,et al.Advances in research on chlorogenic acids at home and abroad[J].J Nucl Agric Sci,2014,28(2):292-301.
[4]王丽萍,郭栋,王果,等.中药绿原酸的研究进展[J].时珍国医国药,2011,4(22):961-963.Wang LP,Guo D,Wang G,et al.Advancement of chlorogenic acid in traditional Chinese medicine[J].Lishizhen Med Mater Med Res,2011,4(22):961-963.
[5]孙玲,樊晓兰,郭绮,等.一测多评法同时测定山银花药材中5种皂苷类成分的含量[J].中国药房,2017,28(18):2546-2549.Sun L,Fan XL,Guo Q,et al.Simultaneous determination of 5 saponins in Lonicera hypoglauca by QAMS method[J].China Pharm,2017,28(18):2546-2549.
[6]陈雨,王奇志,冯煦.忍冬属植物三萜皂苷类化学成分研究进展[J].中草药,2013,44(12):1679-1686.Chen Y,Wang QZ,Feng X.Research progress in triterpenoid saponins in plants of Lonicera L.[J].Chin Trad Herb Drug,2013,44(12):1679-1686.
[7]张璐,曾文雪.HPLC-ELSD同时测定山银花中绿原酸、木犀草苷和川续断皂苷乙的含量[J].江西中医学院学报,2011,23(4):40-42.Zhang L,Zeng WX.HPLC-ELSD determination of chlorogenic acid,galuteolin and dipsacoside B in Flos Lonicerae confusae[J].J Jiangxi Univ Trad Chin Med,2011,23(4):40-42.
[8]刘青芝,王立国,崔伟亮,等.HPLC-ELSD法检查抗感颗粒中山银花皂苷类成分[J].药学研究,2016,35(4):205-208.Liu QZ,Wang LG,Cui WL,et al.Inspection of saponins from Lonicera hypoglauca in Kanggan Granules by HPLC-ELSD[J].J Pharm Res,2016,35(4):205-208.
[9]张艳,周旭美,姚晓东,等.HPLC-ELSD测定绥阳山银花中绿原酸和灰毡毛忍冬皂苷乙的含量[J].遵义医学院学报,2013,36(6):575-577,583.Zhang Y,Zhou XM,Yao XD,et al.Determination of chlorogenic acid and macranthoidin B in Lonicera hypoglauca from Suiyang by HPLC-ELSD method[J].Acta Academ Med Zunyi,2013,36(6):575-577,583.
[10]黄佳,虢小翊,彭国平,等.山银花皂苷提取条件优化研究[J].湖南农业科学,2012,(5):85-87.Huang J,Guo XY,Peng GP,et al.Optimization of conditions for extracting saponins from wild honeysuckle flower[J].Hunan Agric Sci,2012,(5):85-87.
[11]刘婧慧.金银花与山银花质量评价及安全性对比研究[D].北京:北京中医药大学,2014.Liu JH.Comparative study on quality evaluation and safety of Lonicera japonica and Lonicera macranthoides[D].Beijing:Beijing University of Chinese Medicine,2014.
[12]杨斌,丘岳,王柳萍,等.广西山银花绿原酸体外抗炎作用及分子机制研究[J].中国药理学通报,2009,25(4):542-545.Yang B,Qiu Y,Wang LP,et al.Studies on the anti-inflammatory molecular mechanism of chlorogenic acid extracted from Lonicera confusa DC in vitro[J].Chin Pharm Bull,2009,25(4):542-545.
[13]李泮霖,贺利利,李楚源,等.金银花和山银花抗急性口腔炎症作用比较[J].中山大学学报(自然科学版),2016,55(4):118-122.Li BL,He LL,Li CY,et al.Comparison of anti-inflammatory effects between Lonicerae japonicae flos and Lonicera hypoglauca using an acute stomatitis model[J].Acta Sci Nat Univ Suny,2016,55(4):118-122.
[14]刘鸿燕.中药提取物标准管理的问题及对策[J].临床医学研究与实践,2016,1(13):188.Liu HY.Problems and countermeasures of standard management of Chinese herbal extracts[J].Clin Med Res Pract,2016,1(13):188.
[15]任琰,钟根秀,于婧怡.出口植物提取物质量安全现状及监管对策[J].浙江农业科学,2016,57(12):1956-1959,1967.Ren Y,Zhong GX,Yu JY.Quality and safety status of export plant extracts and supervisory measures[J].J Zhejiang Agric Sci,2016,57(12):1956-1959,1967.
[2]张英锋,张立艳,马子川,等.植物绿原酸的研究进展[J].化学教育,2011,32(4):1-6.Zhang YF,Zhang LY,Ma ZC,et al.Advancement of plant chlorogenic acid[J].J Chem Edu,2011,32(4):1-6.
[3]席利莎,木泰华,孙红男,等.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.Xi LS,Mu TH,Sun HN,et al.Advances in research on chlorogenic acids at home and abroad[J].J Nucl Agric Sci,2014,28(2):292-301.
[4]王丽萍,郭栋,王果,等.中药绿原酸的研究进展[J].时珍国医国药,2011,4(22):961-963.Wang LP,Guo D,Wang G,et al.Advancement of chlorogenic acid in traditional Chinese medicine[J].Lishizhen Med Mater Med Res,2011,4(22):961-963.
[5]孙玲,樊晓兰,郭绮,等.一测多评法同时测定山银花药材中5种皂苷类成分的含量[J].中国药房,2017,28(18):2546-2549.Sun L,Fan XL,Guo Q,et al.Simultaneous determination of 5 saponins in Lonicera hypoglauca by QAMS method[J].China Pharm,2017,28(18):2546-2549.
[6]陈雨,王奇志,冯煦.忍冬属植物三萜皂苷类化学成分研究进展[J].中草药,2013,44(12):1679-1686.Chen Y,Wang QZ,Feng X.Research progress in triterpenoid saponins in plants of Lonicera L.[J].Chin Trad Herb Drug,2013,44(12):1679-1686.
[7]张璐,曾文雪.HPLC-ELSD同时测定山银花中绿原酸、木犀草苷和川续断皂苷乙的含量[J].江西中医学院学报,2011,23(4):40-42.Zhang L,Zeng WX.HPLC-ELSD determination of chlorogenic acid,galuteolin and dipsacoside B in Flos Lonicerae confusae[J].J Jiangxi Univ Trad Chin Med,2011,23(4):40-42.
[8]刘青芝,王立国,崔伟亮,等.HPLC-ELSD法检查抗感颗粒中山银花皂苷类成分[J].药学研究,2016,35(4):205-208.Liu QZ,Wang LG,Cui WL,et al.Inspection of saponins from Lonicera hypoglauca in Kanggan Granules by HPLC-ELSD[J].J Pharm Res,2016,35(4):205-208.
[9]张艳,周旭美,姚晓东,等.HPLC-ELSD测定绥阳山银花中绿原酸和灰毡毛忍冬皂苷乙的含量[J].遵义医学院学报,2013,36(6):575-577,583.Zhang Y,Zhou XM,Yao XD,et al.Determination of chlorogenic acid and macranthoidin B in Lonicera hypoglauca from Suiyang by HPLC-ELSD method[J].Acta Academ Med Zunyi,2013,36(6):575-577,583.
[10]黄佳,虢小翊,彭国平,等.山银花皂苷提取条件优化研究[J].湖南农业科学,2012,(5):85-87.Huang J,Guo XY,Peng GP,et al.Optimization of conditions for extracting saponins from wild honeysuckle flower[J].Hunan Agric Sci,2012,(5):85-87.
[11]刘婧慧.金银花与山银花质量评价及安全性对比研究[D].北京:北京中医药大学,2014.Liu JH.Comparative study on quality evaluation and safety of Lonicera japonica and Lonicera macranthoides[D].Beijing:Beijing University of Chinese Medicine,2014.
[12]杨斌,丘岳,王柳萍,等.广西山银花绿原酸体外抗炎作用及分子机制研究[J].中国药理学通报,2009,25(4):542-545.Yang B,Qiu Y,Wang LP,et al.Studies on the anti-inflammatory molecular mechanism of chlorogenic acid extracted from Lonicera confusa DC in vitro[J].Chin Pharm Bull,2009,25(4):542-545.
[13]李泮霖,贺利利,李楚源,等.金银花和山银花抗急性口腔炎症作用比较[J].中山大学学报(自然科学版),2016,55(4):118-122.Li BL,He LL,Li CY,et al.Comparison of anti-inflammatory effects between Lonicerae japonicae flos and Lonicera hypoglauca using an acute stomatitis model[J].Acta Sci Nat Univ Suny,2016,55(4):118-122.
[14]刘鸿燕.中药提取物标准管理的问题及对策[J].临床医学研究与实践,2016,1(13):188.Liu HY.Problems and countermeasures of standard management of Chinese herbal extracts[J].Clin Med Res Pract,2016,1(13):188.
[15]任琰,钟根秀,于婧怡.出口植物提取物质量安全现状及监管对策[J].浙江农业科学,2016,57(12):1956-1959,1967.Ren Y,Zhong GX,Yu JY.Quality and safety status of export plant extracts and supervisory measures[J].J Zhejiang Agric Sci,2016,57(12):1956-1959,1967.