不同品种、产地和栽培基质铁皮石斛的指纹图谱研究
|
摘要
目的采用高效液相色谱法(HPLC)法建立铁皮石斛的指纹图谱,考察不同品种、产地和栽培基质铁皮石斛药材质量的差异情况。方法 HPLC法采用Zorbax SB-C18色谱柱(46 mm×250 mm,5μm),以0.1%磷酸溶液-乙腈为流动相,进行梯度洗脱,筛选检测波长为315 nm,测定了13批铁皮石斛样品的指纹图谱,并通过指纹图谱评价软件和聚类分析等手段,计算相似度,对样品进行评价。结果建立了铁皮石斛的指纹图谱,13批各类铁皮石斛样品共有15个共有峰,相似度评价结果显示:1批样品为0.565,其余均在0.704～0.966之间,聚类分析结果显示13批样品可以聚为三大类。结论建立的指纹图谱方法简便可靠,铁皮石斛的内在质量受品种、栽培基质的影响较大,受产地的影响相对较小。
Objective To establish HPLC fingerprint for Dendrobium officinale and investigate the quality differences of Dendrobium officinale samples from different varieties,geographical origins and cultural substrates.Methods The HPLC separation was performed on a Zorbax(46 mm×250 mm,5μm)column with the mobile phase consisted of 0.1%phosphoric acid-acetonitrile,gradient elution,and the detection wavelength was 315 nm.The fingerprint of 13 batches of samples were tested.The similarity was calculated and the samples were evaluated by means of fingerprint evaluation software and cluster analysis.Results A fingerprint of Dendrobium officinale was established with 15 common peaks.Similarity evaluation results show that:except 1 batch of samples was 0.565,the others were between 0.704 and 0.966.Cluster analysis showed that 13 batches of samples could be grouped into three categories.Conclusion The established method of fingerprint is simple and reliable.The inner quality of Dendrobium officinale was greatly influenced by its variety and culture substrate,and was relatively less affected by the geographical origins.
Objective To establish HPLC fingerprint for Dendrobium officinale and investigate the quality differences of Dendrobium officinale samples from different varieties,geographical origins and cultural substrates.Methods The HPLC separation was performed on a Zorbax(46 mm×250 mm,5μm)column with the mobile phase consisted of 0.1%phosphoric acid-acetonitrile,gradient elution,and the detection wavelength was 315 nm.The fingerprint of 13 batches of samples were tested.The similarity was calculated and the samples were evaluated by means of fingerprint evaluation software and cluster analysis.Results A fingerprint of Dendrobium officinale was established with 15 common peaks.Similarity evaluation results show that:except 1 batch of samples was 0.565,the others were between 0.704 and 0.966.Cluster analysis showed that 13 batches of samples could be grouped into three categories.Conclusion The established method of fingerprint is simple and reliable.The inner quality of Dendrobium officinale was greatly influenced by its variety and culture substrate,and was relatively less affected by the geographical origins.
引文
[1]国家药典委员会.中华人民共和国药典(2015年版):一部[M].北京:中国医药科技出版社,2015:282.
[2]吕圭源,颜美秋,陈素红.铁皮石斛功效相关药理作用研究进展[J].中国中药杂志,2013,38(4):489-493.
[3]黄小鸥,莫可元.铁皮石斛的药理研究新进展[J].中国中医药现代远程教育,2018,16(3):145-148.
[4]林丽珍,许仕锦,杨永军,等.不同生长年限的仿野生铁皮石斛醇提物的抗肿瘤活性比较[J].中药新药与临床药理,2018,29(2):149-154.
[5]吴韵琴,斯金平.铁皮石斛产业现状及可持续发展的探讨[J].中国中药杂志,2010,35(15):2033-2037.
[6]何铁光,卢声仙,王爱勤,等.广西铁皮石斛HPLC指纹图谱研究[J].天然产物研究与开发,2012,24(3):353-358,320.
[7]刘文杰,孙志蓉,杜远,等.不同产地铁皮石斛主要化学成分及指纹图谱研究[J].北京中医药大学学报,2013,36(2):117-120.
[8]杨岚,王红娟,师帅,等. 12种不同产地铁皮石斛指纹图谱研究及重金属元素含量测定[J].西北植物学报,2014,34(10):2078-2084.
[9]魏刚,顺庆生,黄月纯,等. 3种铁皮石斛种源HPLC特征图谱比较研究[J].中药新药与临床药理,2014,25(4):467-471.
[2]吕圭源,颜美秋,陈素红.铁皮石斛功效相关药理作用研究进展[J].中国中药杂志,2013,38(4):489-493.
[3]黄小鸥,莫可元.铁皮石斛的药理研究新进展[J].中国中医药现代远程教育,2018,16(3):145-148.
[4]林丽珍,许仕锦,杨永军,等.不同生长年限的仿野生铁皮石斛醇提物的抗肿瘤活性比较[J].中药新药与临床药理,2018,29(2):149-154.
[5]吴韵琴,斯金平.铁皮石斛产业现状及可持续发展的探讨[J].中国中药杂志,2010,35(15):2033-2037.
[6]何铁光,卢声仙,王爱勤,等.广西铁皮石斛HPLC指纹图谱研究[J].天然产物研究与开发,2012,24(3):353-358,320.
[7]刘文杰,孙志蓉,杜远,等.不同产地铁皮石斛主要化学成分及指纹图谱研究[J].北京中医药大学学报,2013,36(2):117-120.
[8]杨岚,王红娟,师帅,等. 12种不同产地铁皮石斛指纹图谱研究及重金属元素含量测定[J].西北植物学报,2014,34(10):2078-2084.
[9]魏刚,顺庆生,黄月纯,等. 3种铁皮石斛种源HPLC特征图谱比较研究[J].中药新药与临床药理,2014,25(4):467-471.