西瓜种子大小全基因组关联分析
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摘要
试验调查分析62份不同西瓜种质资源种子大小性状,包括种子长度、种子宽度、种子厚度、种形指数、种子千粒重、单瓜种子数等。针对性状结果展开数据分析,以主成分分析、相关性分析、聚类分析等不同统计学方法分析不同品种特征近似程度。种子大小遗传多样性分析结果表明,62份西瓜种质资源中,种子长度、种子宽度、种子厚度、种形指数、种子千粒重、单瓜种子数多样性指数均在4.20以上,呈较高多样性;通过全基因组关联分析发现,共检测到7个与西瓜种子长度相关的SNP位点和11个与西瓜种子宽度相关的SNP位点,其中4、7号染色体上2个与西瓜种子长度相关的SNP达显著水平,4个与西瓜种子宽度关联的SNP位点达显著水平,分别位于1、4、7、10号染色体。
In the research, the seed size characters of 62 watermelon germplasm resources were investigated, including seed length, seed width, seed thickness, seed shape index, the mass of 1000 grains and seeds number per watermelon. According to traits results, comprehensive data analysis had been made including principal component analysis, correlation analysis, cluster analysis and so on and also different statistical methods were used to get the approximate degree of the characteristics of different varieties. The results of seed size genetic diversity analysis showed that in 62 watermelon germplasm resources, the diversity index of seed length, seed width, seed thickness, seed shape index,the mass of 1000 grains and seed number of per watermelon was all above 4.20 with high diversity.Genome-wide association analysis showed that seven SNPs related to seed length and 11 SNPs related to seed width were detected in watermelon. Among them, the two SNPs on chromosomes No.4,and No.7 were significantly correlated with the seed length of watermelon and four SNPs associated with seed width were significantly on chromosomes No.1, No.4, No.7 and No. 10, respectively.
In the research, the seed size characters of 62 watermelon germplasm resources were investigated, including seed length, seed width, seed thickness, seed shape index, the mass of 1000 grains and seeds number per watermelon. According to traits results, comprehensive data analysis had been made including principal component analysis, correlation analysis, cluster analysis and so on and also different statistical methods were used to get the approximate degree of the characteristics of different varieties. The results of seed size genetic diversity analysis showed that in 62 watermelon germplasm resources, the diversity index of seed length, seed width, seed thickness, seed shape index,the mass of 1000 grains and seed number of per watermelon was all above 4.20 with high diversity.Genome-wide association analysis showed that seven SNPs related to seed length and 11 SNPs related to seed width were detected in watermelon. Among them, the two SNPs on chromosomes No.4,and No.7 were significantly correlated with the seed length of watermelon and four SNPs associated with seed width were significantly on chromosomes No.1, No.4, No.7 and No. 10, respectively.
引文
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[2] Lee S J, Shin J S, Park K W, et al. Detection of genetic diversity using RAPD-PCR and sugar analysis in watermelon[Citrullus lanantus(Thunb.)Mansf.] germplasm[J]. Theoretical and Applied Genetics, 1996, 92(6):719-725.
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[9]林珲,陈朝文,温庆放,等.丝瓜种质资源遗传多样性分析[J].分子植物育种, 2017, 15(9):3759-3766.
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[12]周慧文,卢丙洋,马鸿艳,等.西瓜种子大小形状相关QTL分析[J].园艺学报, 2016, 43(4):715-723.
[13]孙玉芬.基于网格方法的聚类算法研究[D].武汉:华中科技大学, 2006.
[14]马双武,刘君璞.西瓜种质资源描述规范和数据标准[J].北京:中国农业出版社, 2005.
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[16]周先林,樊丹,朱艳梅,等.制种西瓜产籽量与叶面积指数及留叶量的相关性分析[J].中国瓜菜, 2012, 25(1):36-38.
[17]崔光泉.西瓜数量性状间的相关聚类分析[J].中国西瓜甜瓜,1989(2):10-13.
[18]王敏,张圣平,苗晗,等.瓜类种子性状遗传及分子生物学研究进展[J].中国蔬菜, 2013(14):1-8.
[19] Ren Y, Mcgregor C, Zhang Y, et al. An integrated genetic map based on four mapping populations and quantitative trait loci associated with economically important traits in watermelon(Citrullus lanatus)[J]. BMC Plant Biology, 2014, 14(1):33.
[2] Lee S J, Shin J S, Park K W, et al. Detection of genetic diversity using RAPD-PCR and sugar analysis in watermelon[Citrullus lanantus(Thunb.)Mansf.] germplasm[J]. Theoretical and Applied Genetics, 1996, 92(6):719-725.
[3] Eriksson O. Game theory provides no explanation for seed size variation in grasslands[J]. Oecologia, 2005, 144(1):98-105.
[4] Klinkhamer B G, Jong T J. Plangt size and seed production in the monocarpic pcrennial Cynoglossum officinale L.[J]. New Phytol,1987, 106:773-783.
[5]纪海波,张玉鑫,李玉明,等.西瓜种质资源主要性状的表型多样性[J].西北农林科技大学学报:自然科学版, 2013, 41(8):155-160.
[6]潘存祥,许勇,纪海波,等.西瓜种质资源表型多样性及聚类分析[J].植物遗传资源学报, 2015, 16(1):59-63.
[7]尚建立,王吉明,郭琳琳,等.西瓜种质资源主要植物学性状的遗传多样性及相关性分析[J].植物遗传资源学报, 2012, 13(1):11-15, 21.
[8]杨志玲,杨旭,谭梓峰,等.厚朴不同种源及家系种子性状的变异[J].中南林业科技大学学报, 2009, 29(5):49-55.
[9]林珲,陈朝文,温庆放,等.丝瓜种质资源遗传多样性分析[J].分子植物育种, 2017, 15(9):3759-3766.
[10] Huang X, Han B. Natural variations and genome-wide association studies in crop plants[J]. Annual Review of Plant Biology, 2014,65:531-551.
[11]刘传奇,高鹏,栾非时.西瓜遗传图谱构建及果实相关性状QTL分析[J].中国农业科学, 2014, 47(14):2814-2829.
[12]周慧文,卢丙洋,马鸿艳,等.西瓜种子大小形状相关QTL分析[J].园艺学报, 2016, 43(4):715-723.
[13]孙玉芬.基于网格方法的聚类算法研究[D].武汉:华中科技大学, 2006.
[14]马双武,刘君璞.西瓜种质资源描述规范和数据标准[J].北京:中国农业出版社, 2005.
[15]尚建立,王吉明,马双武.西瓜种子表型性状的遗传多样性分析[J].中国瓜菜, 2013, 26(3):10-12.
[16]周先林,樊丹,朱艳梅,等.制种西瓜产籽量与叶面积指数及留叶量的相关性分析[J].中国瓜菜, 2012, 25(1):36-38.
[17]崔光泉.西瓜数量性状间的相关聚类分析[J].中国西瓜甜瓜,1989(2):10-13.
[18]王敏,张圣平,苗晗,等.瓜类种子性状遗传及分子生物学研究进展[J].中国蔬菜, 2013(14):1-8.
[19] Ren Y, Mcgregor C, Zhang Y, et al. An integrated genetic map based on four mapping populations and quantitative trait loci associated with economically important traits in watermelon(Citrullus lanatus)[J]. BMC Plant Biology, 2014, 14(1):33.
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