外源SA对葡萄叶片抗氧化酶活性的影响
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摘要
通过外源喷施水杨酸(Salicylic acid,SA),以酿酒葡萄品种赤霞珠(VitisviniferaL. cv. Cabernet Sauvignon)为试材,初步探讨了外源SA对赤霞珠葡萄叶片超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性以及膜质过氧化产物丙二醛(MDA)含量的影响。结果表明:水杨酸处理后,超氧化物歧化酶(SOD)的活性表现出先升高后又下降的趋势;过氧化氢酶(CAT)的活性表现为上升趋势,但是不同浓度SA表现情况不同,较低浓度如1.00 mmol/L处理时表现最好,而高浓度如5.00 mmol/L时则有抑制的作用;膜质过氧化产物丙二醛(MDA)的含量较对照有下降的趋势,这表明外源SA可有效减少MDA的积累,从而减轻活性氧积累带来的伤害。
In this experiment,through spurting exogenous SA,the wine varieties of grape(Vitisvinifera L. cv. Cabernet Sauvignon)leaves were used as materials,exploration preliminary theinfluences of exogenous SA on the change of SOD,CAT activity and MDA content. The result shows: With increasing conduct time,antioxidant system activity of SOD enhanced first and then slightly reduced; CAT activity obviously increased,but the different concentration have different result,the low concentration such as 1.00 mmol/L had a good result while 5.00 mmol/L had a bad result; MDA content is lower than the compare. It explained that the exogenous SA could reduce the content of MDA,and then reduce the hurt of accumulated active oxygen.
In this experiment,through spurting exogenous SA,the wine varieties of grape(Vitisvinifera L. cv. Cabernet Sauvignon)leaves were used as materials,exploration preliminary theinfluences of exogenous SA on the change of SOD,CAT activity and MDA content. The result shows: With increasing conduct time,antioxidant system activity of SOD enhanced first and then slightly reduced; CAT activity obviously increased,but the different concentration have different result,the low concentration such as 1.00 mmol/L had a good result while 5.00 mmol/L had a bad result; MDA content is lower than the compare. It explained that the exogenous SA could reduce the content of MDA,and then reduce the hurt of accumulated active oxygen.
引文
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[2]刘林德,姚敦义.植物激素的概念及其新成员[J].生物学通报,2002,37(8):18-20.
[3]李品明,杨丙贤,孙玉芳,等.高温胁迫对黄连幼苗活性氧代谢及保护酶活性的影响[J].安徽农业科学,2011,39(18):10796-10798.
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[12]詹妍妮,郁松林,陈培琴,等.热锻炼与外源水杨酸对葡萄叶肉细胞膜脂过氧化的影响[J].石河子大学学报(自然科学版),2005,23(5):597-600.
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[15]何亚丽,刘友良.水杨酸的热锻炼诱导的高羊茅幼苗的耐热性与抗氧化的关系[J].植物生理与分子生物学报,2002,28(2):89-95.
[16]Dat J F,Leope Delgado,et al. Parallel change in H2O2andcatalaseduring thermotolerance induced by salicylic acid or heat acclimationin musturdseeldlings [J].PlantPhysiology,1998,116:1351-1357.
[17]Dat JF,Leopez Delgado,et al. Change in salicylic acid and antioxidantsduring induced thermotoleranc in musturdplant[J].PlantPhysiology,1998,118:1455-1461.
[18]Lopez Relgado H,DatJF,et al.ScottInductionof thermotolerancein potato microplants by acetylsalicylic acid and H2O2[J].Journalof Experimental Botany,1998,49(321):713-720.
[19]王利军,黄卫东.葡萄幼苗高温锻炼过程中与水杨酸相关的信号传递的初步研究[J].植物学通报,2002,19(6):710-715.
[20]王利军,黄卫东,李家永.水杨酸对葡萄幼苗叶片膜脂过氧化的影响[J].中国农业科学,2003,36(9):1076-1080.
[2]刘林德,姚敦义.植物激素的概念及其新成员[J].生物学通报,2002,37(8):18-20.
[3]李品明,杨丙贤,孙玉芳,等.高温胁迫对黄连幼苗活性氧代谢及保护酶活性的影响[J].安徽农业科学,2011,39(18):10796-10798.
[4]章艳玲,程纪伦,李关荣.栽培条件对半夏叶片POD、SOD、CAT活性的影响[J].中国农学通报,2012,28(4):211-214.
[5]李会云,郭修武.盐胁迫对葡萄砧木叶片保护酶活性和丙二醛含量的影响[J].果树学报,2008,25(2):240-243.
[6]孙国荣,彭永臻,阎秀峰,等.干旱胁迫对白桦实生苗保护酶活性及膜质过氧化作用的影响[J].林业科学,2003,39(1):165-167.
[7]彭立新,周黎君,冯涛,等. 盐胁迫对沙枣幼苗抗氧化酶活性和膜脂过氧化的影响[J].天津农学院学报,2009,16(4):1-4.
[8]黄爱霞,佘小平.水杨酸对黄瓜幼苗抗冷性的影响[J].陕西师范大学学报,2003,31(3):107-109.
[9]Song S-Q (宋士清),Liu W (刘微),Guo S-R (郭世荣),et al. Salt resistance and its mechanism of cucumber under effects of exogenous chemical activators. Chinese Journal of Applied Ecology (应用生态学报),2006,17(10):1871-1876(in Chinese).
[10]王伟英,林江波,邹晖,等.水杨酸处理对水仙株型及抗氧化物酶活性的影响[J].中国农学通报,2009,25(14):157-160.
[11]王丽,王燕凌,廖康.外源水杨酸处理对全球红葡萄植株抗寒性的影响[J].新疆农业大学学报,2005,28(2):51-54.
[12]詹妍妮,郁松林,陈培琴,等.热锻炼与外源水杨酸对葡萄叶肉细胞膜脂过氧化的影响[J].石河子大学学报(自然科学版),2005,23(5):597-600.
[13]RaoMV,PaliyathG,etal.Influence of salisylicacid on H2O2 production,oxiduction,oxidativestress and H2O2-me-tabolizating enzymes[J].Plant Physiology,1997,115:137-149.
[14]李兆亮,原永兵.黄瓜细胞中水杨酸的信号传递研究[J].植物学报,1998,40(5):356-361.
[15]何亚丽,刘友良.水杨酸的热锻炼诱导的高羊茅幼苗的耐热性与抗氧化的关系[J].植物生理与分子生物学报,2002,28(2):89-95.
[16]Dat J F,Leope Delgado,et al. Parallel change in H2O2andcatalaseduring thermotolerance induced by salicylic acid or heat acclimationin musturdseeldlings [J].PlantPhysiology,1998,116:1351-1357.
[17]Dat JF,Leopez Delgado,et al. Change in salicylic acid and antioxidantsduring induced thermotoleranc in musturdplant[J].PlantPhysiology,1998,118:1455-1461.
[18]Lopez Relgado H,DatJF,et al.ScottInductionof thermotolerancein potato microplants by acetylsalicylic acid and H2O2[J].Journalof Experimental Botany,1998,49(321):713-720.
[19]王利军,黄卫东.葡萄幼苗高温锻炼过程中与水杨酸相关的信号传递的初步研究[J].植物学通报,2002,19(6):710-715.
[20]王利军,黄卫东,李家永.水杨酸对葡萄幼苗叶片膜脂过氧化的影响[J].中国农业科学,2003,36(9):1076-1080.