酸碱胁迫对舞毒蛾幼虫营养与总抗氧化力的影响
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摘要
为探究舞毒蛾幼虫对酸碱饲料胁迫的响应机制,采用不同酸碱度磷酸缓冲液(PBS6、PBS7、PBS8)配制饲料,对4龄舞毒蛾幼虫进行饲喂处理。测定分析了舞毒蛾幼虫的营养取食指标、营养成分含量及总抗氧化力。以蒸馏水配制的饲料作为对照。结果表明:饲料酸碱度可以抑制舞毒蛾幼虫取食量、粪便量、体重增长量及同化率;饲料酸碱性对碳水化合物含量和脂质含量均有显著抑制作用;而对可溶性蛋白含量和海藻糖含量无显著性影响,酸碱饲料胁迫可以提高舞毒蛾幼虫总抗氧化力。在酸碱胁迫条件下,碳水化合物和脂质含量呈下降趋势,此而总抗氧化力呈上升趋势。
In order to explore how gypsy moth larvae respond to acid-base diets stress,artificial diets were prepared with phosphate buffer of different pH value,and named respectively as PBS6,PBS7 and PBS8 according to the p H value of phosphate buffer. The fourth instar gypsy moth larvae were fed with those acid-base diets. Afterwards,food consumption,nutrient composition and total antioxidant capacity of gypsy moth larvae were detected. Results indicated that alimental p H could affect feeding amount,fecal amount,weight growth and assimilation rate of gypsy moth larvae; soluble protein content and trehalose content were not significantly affected by acidity and alkalinity of diets,but carbohydrate content and lipid content did. Moreover,the total antioxidant capacity was increased by pH stress. In conclusion,carbohydrates content and lipids content decreased by the alimental p H stress,however,the total antioxidant capacity was significantly increased.
In order to explore how gypsy moth larvae respond to acid-base diets stress,artificial diets were prepared with phosphate buffer of different pH value,and named respectively as PBS6,PBS7 and PBS8 according to the p H value of phosphate buffer. The fourth instar gypsy moth larvae were fed with those acid-base diets. Afterwards,food consumption,nutrient composition and total antioxidant capacity of gypsy moth larvae were detected. Results indicated that alimental p H could affect feeding amount,fecal amount,weight growth and assimilation rate of gypsy moth larvae; soluble protein content and trehalose content were not significantly affected by acidity and alkalinity of diets,but carbohydrate content and lipid content did. Moreover,the total antioxidant capacity was increased by pH stress. In conclusion,carbohydrates content and lipids content decreased by the alimental p H stress,however,the total antioxidant capacity was significantly increased.
引文
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[25]陈龙,周晓榕,高利军,等.沙葱萤叶甲成虫越夏期间糖类、蛋白及脂肪含量的变化[J].昆虫学报,2018,61(7):808-814.
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[28]于彩虹,梁晓贺,卢丹,等.几种植物源化合物对棉铃虫海藻糖酶活性及相关碳水化合物含量的影响[J].昆虫学报,2011,54(1):41-49.
[29]张方明,曾健勇,吴玥,等,舞毒蛾幼虫对阿维·杀铃脲复合剂胁迫的响应机制[J].黑龙江畜牧兽医,2019(1):106-110.
[30]周桂莲,杨慧霞.小麦抗旱型鉴定的生理生化指标及其分析评价[J].干旱地区农业研究,1996,14(2):65-70.
[31]王跃,李丽君,郭亚平,等.镉长期暴露对中华稻蝗抗氧化机制的影响[J].农业环境科学学报,2011,30(3):443-448.
[2]张琪慧,孙丽丽,刘鹏,等.转舞毒蛾CYP6B53基因果蝇品系对2种杀虫剂的胁迫响应[J].吉林农业大学学报,2018,40(2):152-156.
[3]张国财.舞毒蛾防治技术的研究[D].哈尔滨:东北林业大学,2002.
[4]方三阳.森林昆虫学[M].哈尔滨:东北林业大学出版社,1988:121-125.
[5]陈全涉.舞毒蛾测报与防治方法的初步研究[J].东北林业大学学报,1999,27(1):64-66.
[6]姜礅,狄贵秋,严善春.Cd胁迫对舞毒蛾幼虫食物利用的影响及其Cd的外泌机制[J].应用生态学报,2018,29(6):1969-1974.
[7]孟建玉.UV胁迫下棉铃虫生理生化响应及蛋白质组学研究[D].武汉:华中农业大学,2010.
[8]张治安.镉胁迫对大豆下胚轴保护酶活性的影响[D].吉林农业大学学报,2005,27(3):237-240.
[9]王亚军,邹传山,杨璟,等.3种植物次生代谢物质对舞毒蛾生长发育的影响[J].吉林农业大学学报,2018,40(2):145-151.
[10]潘正军,沈丹,茆佳,等.光照强度和p H对西藏卤虫孵化率的影响[J].内蒙古师范大学学报(自然科学版),2019,48(2):159-162.
[11]彭东琴.酸雨处理对4种亚热带树种叶片p H与生长特性的影响[D].杭州:浙江农林大学,2013.
[12]于飞,曾鑫年,张帅,等.取食量对昆虫生长发育影响的研究[J].广东农业科学,2004(1):42-44
[13]曾健勇,张方明,吴玥,等.阿维菌素与杀铃脲对舞毒蛾幼虫的联合作用机制[J].林业科学,2018,54(12):110-115.
[14]丁吉同,唐桦,阿地力·沙塔尔,等.4种植物源杀虫剂对亚洲型舞毒蛾幼虫的毒性与拒食作用[J].南京林业大学学报(自然科学版),2013,37(4):80-84.
[15]吕春鹤.白屈菜提取物对舞毒蛾酶活性抑制作用及机制的研究[D].哈尔滨:东北林业大学,2017.
[16] Bradford M M.A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(1):248-254.
[17] Roe J H.The determination of sugar in blood and spinal fluid with anthrone reagent[J].Bio Chemistry,1955,212(1):335.
[18]雷芳,张桂芬,万方浩,等.寄主转换对B型烟粉虱和温室粉虱海藻糖含量和海藻糖酶活性的影响[J].中国农业科学,2006,39(7):1387-1394.
[19] Simek P,Sula J,Kostal V.Physiology of drought tolerance andcold hardiness of the mediterranean tiger moth Cymbalophorapudica during summer diapause[J]. Insect Physiol,1998,44(2):165-173.
[20]赵军霞.土壤酸碱性与植物的生长[J].内蒙古农业科技,2003(6):33-42.
[21]邵世光,阎斌伦,许云华,等.Cd2+对条斑紫菜的胁迫作用[J].河南师范大学学报(自然科学版),2006,34(2):113-116.
[22] Li Hongmei,Ren Chunhua,Jiang Xiao,et al. Na+/H+exchanger(NHE)in pacific white shrimp molecular cloning transcriptional response to acidity[J]. PLoS one,2019,14(2):e0212887.
[23]郭苏帆.高温对韭菜迟眼蕈蚊和异迟眼蕈蚊生长发育及生理代谢的影响[D].兰州:甘肃农业大学,2018.
[24]张祥,胡大鹏,李远,等.土壤水分亏缺对Bt棉铃壳杀虫蛋白表达的影响[J].中国农业科学,2017,50(4):640-647.
[25]陈龙,周晓榕,高利军,等.沙葱萤叶甲成虫越夏期间糖类、蛋白及脂肪含量的变化[J].昆虫学报,2018,61(7):808-814.
[26]张雄帅,周国娜,高宝嘉.油松毛虫体内酶系对油松诱导抗性的响应机制[J].林业科学,2014,50(10):181-187.
[27] Yazdanfar H,Ghodskhah Dariaii M,Jalali Sendi J.The effects of host plants on the feeding indices and chemicalactivities of elm leaf beetle,Xanthogaleruca luteola(Muller)(Coleoptera:Chrysomelidae)[J]. Iran Agricultural Research,2016,35(2):81-87.
[28]于彩虹,梁晓贺,卢丹,等.几种植物源化合物对棉铃虫海藻糖酶活性及相关碳水化合物含量的影响[J].昆虫学报,2011,54(1):41-49.
[29]张方明,曾健勇,吴玥,等,舞毒蛾幼虫对阿维·杀铃脲复合剂胁迫的响应机制[J].黑龙江畜牧兽医,2019(1):106-110.
[30]周桂莲,杨慧霞.小麦抗旱型鉴定的生理生化指标及其分析评价[J].干旱地区农业研究,1996,14(2):65-70.
[31]王跃,李丽君,郭亚平,等.镉长期暴露对中华稻蝗抗氧化机制的影响[J].农业环境科学学报,2011,30(3):443-448.
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