灰枣优系抗寒性综合评价
|
摘要
【目的】以灰枣和新疆林业科学院新选育的3个灰枣优系(灰实2、灰实7和灰实8)为试验材料,对其枝条组织在低温胁迫下的相对电导率、可溶性糖、可溶性蛋白、丙二醛含量及游离脯氨酸等5个抗寒性生理指标展开研究,以期为灰枣耐寒品种的选育推广提供参考。【方法】于2017年1月下旬,选取长势一致的1年生休眠二次枝进行低温胁迫试验,胁迫程度分为-15、-20、-25、-30、-35、-40℃共6个梯度,持续胁迫时间为12 h,胁迫后以5℃/h上升至0℃后恒温静置12 h测定各项指标。【结果】结合Logistic方程计算得出,灰实2、灰实7、灰实8和灰枣低温半致死温度分别为-11.48、-8.82、-9.30和-10.80℃。同时,低温胁迫过程中,可溶性糖含量均呈现出先升后降的趋势,且均在-30℃时达到最大值。可溶性蛋白含量呈现"S"型的变化趋势。丙二醛含量呈现"低-高-低-高"波浪型变化趋势,灰实2和灰枣在-35℃时含量最高,达到11μmol/g以上,灰实7和灰实8在-40℃时含量最高,为10.42～10.71μmol/g。灰实7和灰实8的游离脯氨酸含量变化幅度较大,达到3.89～3.93 mg/g;灰实2和灰枣的变化幅度较小,为3.22～3.31 mg/g。整体上看,低温处理下灰枣及其3个优系的游离脯氨酸含量变化表现为先上升后下降的变化趋势。【结论】通过抗寒因子的综合隶属度计算显示,灰实2的平均隶属度最高,为0.482;灰枣次之,为0.478;灰实8为0.476;灰实7最低,为0.456。按照平均隶属度抗寒性分级法分级,灰实2、灰实7、灰实8和灰枣的综合隶属度在0.40～0.59之间,抗寒性均为Ⅲ级,中抗,其抗寒性能力大小综合排序为:灰实2>灰枣>灰实8>灰实7。
【Objective】In this study,Zizyphus jujuba'Huizao'and its three excellent strains(grey experiment 2,7,8) selected by Xinjiang academy of forestry were used as tested materials.The effects of low temperature stress on the physiological cold resistance indices of the branches of Zizyphus jujuba'Huizao',including cold relative electrical conductivity,water soluble sugar,soluble protein,malonaldehyde content and free proline content were studied to provide a reference for the breeding and promotion of cold resistant varieties of Zizyphus jujuba'Huizao'.【Method】In late January 2017,one year old dormant secondary branches were selected for low temperature stress test,the degree of stress was divided into six gradients:-15,-20,-25,-30,-35,-40 ℃,the stress time was 12 hours and then rise up to0 ℃ at 5 ℃ per hour,and after 12 hours constant temperature standing,the indices were measured.【Result】Combined with the logistic equation,the semi lethal temperatures of grey experiment 2,7,8 and Zizyphus jujuba'Huizao'were-11.48,-8.82,-9.30 and-10.80 ℃.At the same time,the content of water soluble sugar showed a trend of rising at first and then decreasing,the different varieties or excellent strains jujube trees reached the maximum value at-30 ℃.The soluble protein content showed a trend of'S'type.The MDA content in the branches of different excellent strains showed a'low-high-low-high'wave trend,the content of MDA of grey experiment 2 and Zizyphus jujuba'Huizao'reached the highest at-35 ℃,with 11μmol/g,and the MDA content of grey experiment 7 and 8 reached the highest at-40 ℃,with 10.42-10.71 μmol/g.The free proline in grey experiment 7 and grey experiment 8 changed greatly,reaching 3.89-3.93 mg/g,while the variation range of grey experiment 2 and Zizyphus jujuba 'Huizao'was smaller,which was 3.22-3.31 mg/g.As a whole,the change of free proline content showed a trend of increasing first and the decreasing at low temperature.【Conclusion】Through the calculation of the comprehensive membership degree of cold resistant factors,the average membership degree of grey experiment 2 was the highest,which was 0.482,Zizyphus jujuba'Huizao'followed by 0.478,grey experiment 8 was 0.476,and grey experiment 7 was the lowest,with 0.456.According to average membership degree and cold resistance classification method,the comprehensive subordinate degree of grey experiment 2,7,8 and Zizyphus jujuba'Huizao'was between 0.40-0.59,their cold resistance was grade Ⅲ,medium resistance,and the comprehensive rank of its cold resistance ability was in order of grey experiment 2>Zizyphus jujuba'Huizao'>grey experiment 8>grey experiment 7.
【Objective】In this study,Zizyphus jujuba'Huizao'and its three excellent strains(grey experiment 2,7,8) selected by Xinjiang academy of forestry were used as tested materials.The effects of low temperature stress on the physiological cold resistance indices of the branches of Zizyphus jujuba'Huizao',including cold relative electrical conductivity,water soluble sugar,soluble protein,malonaldehyde content and free proline content were studied to provide a reference for the breeding and promotion of cold resistant varieties of Zizyphus jujuba'Huizao'.【Method】In late January 2017,one year old dormant secondary branches were selected for low temperature stress test,the degree of stress was divided into six gradients:-15,-20,-25,-30,-35,-40 ℃,the stress time was 12 hours and then rise up to0 ℃ at 5 ℃ per hour,and after 12 hours constant temperature standing,the indices were measured.【Result】Combined with the logistic equation,the semi lethal temperatures of grey experiment 2,7,8 and Zizyphus jujuba'Huizao'were-11.48,-8.82,-9.30 and-10.80 ℃.At the same time,the content of water soluble sugar showed a trend of rising at first and then decreasing,the different varieties or excellent strains jujube trees reached the maximum value at-30 ℃.The soluble protein content showed a trend of'S'type.The MDA content in the branches of different excellent strains showed a'low-high-low-high'wave trend,the content of MDA of grey experiment 2 and Zizyphus jujuba'Huizao'reached the highest at-35 ℃,with 11μmol/g,and the MDA content of grey experiment 7 and 8 reached the highest at-40 ℃,with 10.42-10.71 μmol/g.The free proline in grey experiment 7 and grey experiment 8 changed greatly,reaching 3.89-3.93 mg/g,while the variation range of grey experiment 2 and Zizyphus jujuba 'Huizao'was smaller,which was 3.22-3.31 mg/g.As a whole,the change of free proline content showed a trend of increasing first and the decreasing at low temperature.【Conclusion】Through the calculation of the comprehensive membership degree of cold resistant factors,the average membership degree of grey experiment 2 was the highest,which was 0.482,Zizyphus jujuba'Huizao'followed by 0.478,grey experiment 8 was 0.476,and grey experiment 7 was the lowest,with 0.456.According to average membership degree and cold resistance classification method,the comprehensive subordinate degree of grey experiment 2,7,8 and Zizyphus jujuba'Huizao'was between 0.40-0.59,their cold resistance was grade Ⅲ,medium resistance,and the comprehensive rank of its cold resistance ability was in order of grey experiment 2>Zizyphus jujuba'Huizao'>grey experiment 8>grey experiment 7.
引文
[1]Chen C F,Lee J F,Wang D,et al. Water extract of Zizyphus jujuba attenuates ischemia/reperfusion induced liver injury in rats(PP106)[J]. Transplantation Proceedings,2010,42(3):741-743.
[2]Li J W,Ding S D,Ding X L. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube[J]. Process Biochemistry,2005,40(11):3607-3613.
[3]Shen X C,Tang Y P,Yang R H,et al. The protective effect of Zizyphus jujube fruit on carbon tetrachloride induced hepatic injury in mice by antioxidative activities[J]. Journal of Ethnopharmacology,2009,122(3):555-560.
[4]冯会丽,吴正保,史彦江,等.基于因子分析的灰枣优良无性系果实品质评价[J].食品科学,2016,37(9):77-81.
[5]郭佳欢,潘存德,冯会丽,等.枣麦间作系统中冬小麦的冠层光分布特征及产量研究[J].中国生态农业学报,2016,24(2):183-191.
[6]沈洪波,陈学森,张艳敏.果树抗寒性的遗传与育种研究进展[J].果树学报,2002,19(5):292-297.
[7]Gonzálezaguilar G A,Tiznadohernández M E,Zavaletagatica R,et al. Methyl jasmonate treatment reduce chilling injury and activate the defense response of guava fruits[J]. Biochemical and Biophysical Research Communications,2004,313(3):694-701.
[8]李小琴,彭明俊,段安安,等.低温胁迫对8个核桃无性系抗寒生理指标的影响[J].西北林学院学报,2012,27(6):12-15.
[9]Khedr A H A,Abbas M A,Wahid A A A,et al. Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress[J]. Journal of Experiment Botany,2003,54(392):2553-2553.
[10]李淑玲,冯建荣,李亚兰,等.引入石河子地区的苹果品种抗寒性检测[J].果树学报,2012,29(6):1010-1016.
[11]王玮,李红旭,赵明新,等. 7个梨品种的低温半致死温度及耐寒性评价[J].果树学报,2015,32(5):860-865.
[12]李勃,刘成连,杨瑞红,等.樱桃砧木抗寒性鉴定[J].果树学报,2006,23(2):196-199.
[13]高振,翟衡,孙鲁龙,等.基于温度-伤害度关系分析酿酒葡萄根系及芽抗寒性[J].应用生态学报,2014,25(4):983-990.
[14]刘杜玲,张博勇,彭少兵,等.基于早时核桃不同品种叶片组织结构的抗寒性划分[J].果树学报,2012,29(2):205-211.
[15]高京草,王长柱,王进国,等.枣树抗寒性测定方法研究[J].西北林学院学报,2011,26(5):72-75.
[16]王爱芳,张钢,魏士春,等.不同发育时期樟子松(Pinus sylvestris L. var. mongolica Litv.)的电阻抗参数与抗寒性的关系[J].生态学报,2008,28(11):5741-5749.
[17]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[18]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:144-148.
[19]Theocharis A,Clément C,Barka E A,et al. Physiological and molecular changes in plants grown at low temperatures[J]. Planta,2012,235(6):1091-1091.
[20]史清华,高建设,王军,等. 5个杨树无性系抗寒性的测定与评价[J].西北植物学报,2003,23(11):1937-1941.
[21]张文娥,王飞,潘学军.应用隶属函数法综合评价葡萄种间抗寒性[J].果树学报,2007,24(6):849-853.
[22]Repo T,Zhang G,Ryypp9 A,et al. The electrical impedance spectroscopy of Scots pine(Pinus sylvestris L.)Shoots in relation to cold acclimation[J]. Journal of Experimental Botany,2000,51(353):2095-2107.
[23]Nicese F P,Mancuso S,Masi E,et al. Comparing fractal analysis,electrical impendence and electrolyte leakage for the assessment of cold tolerance in Callistemon and Grevillea spp.[J]. Journal of Horticultural Science&Biotechnology,2004,79(4):627-632.
[24]Jan N,Andrabi K. Cold resistance in plants:A mystery unresolved[J]. Electronic Journal of Biotechnology,2009,12(3):1-15.
[25]Sakai A. Freezing resistance of ornamental trees and shrubs[J].Journal of American for Horticultural Science,1982,107(4):572-581.
[26]玉苏甫·阿不力提甫,阿依古丽·铁木儿,帕提曼·阿布都热合曼.利用隶属函数法综合评价梨砧木抗寒性[J].中国农业大学学报,2014,19(3):121-129.
[27]Castonguay Y,Bertrand A,Michaud R,et al. Cold-induced biochemical and molecular changes in populations selectively improved for freezing tolerance[J]. Crop Science,2011,51(5):2132-2144.
[2]Li J W,Ding S D,Ding X L. Comparison of antioxidant capacities of extracts from five cultivars of Chinese jujube[J]. Process Biochemistry,2005,40(11):3607-3613.
[3]Shen X C,Tang Y P,Yang R H,et al. The protective effect of Zizyphus jujube fruit on carbon tetrachloride induced hepatic injury in mice by antioxidative activities[J]. Journal of Ethnopharmacology,2009,122(3):555-560.
[4]冯会丽,吴正保,史彦江,等.基于因子分析的灰枣优良无性系果实品质评价[J].食品科学,2016,37(9):77-81.
[5]郭佳欢,潘存德,冯会丽,等.枣麦间作系统中冬小麦的冠层光分布特征及产量研究[J].中国生态农业学报,2016,24(2):183-191.
[6]沈洪波,陈学森,张艳敏.果树抗寒性的遗传与育种研究进展[J].果树学报,2002,19(5):292-297.
[7]Gonzálezaguilar G A,Tiznadohernández M E,Zavaletagatica R,et al. Methyl jasmonate treatment reduce chilling injury and activate the defense response of guava fruits[J]. Biochemical and Biophysical Research Communications,2004,313(3):694-701.
[8]李小琴,彭明俊,段安安,等.低温胁迫对8个核桃无性系抗寒生理指标的影响[J].西北林学院学报,2012,27(6):12-15.
[9]Khedr A H A,Abbas M A,Wahid A A A,et al. Proline induces the expression of salt-stress-responsive proteins and may improve the adaptation of Pancratium maritimum L. to salt-stress[J]. Journal of Experiment Botany,2003,54(392):2553-2553.
[10]李淑玲,冯建荣,李亚兰,等.引入石河子地区的苹果品种抗寒性检测[J].果树学报,2012,29(6):1010-1016.
[11]王玮,李红旭,赵明新,等. 7个梨品种的低温半致死温度及耐寒性评价[J].果树学报,2015,32(5):860-865.
[12]李勃,刘成连,杨瑞红,等.樱桃砧木抗寒性鉴定[J].果树学报,2006,23(2):196-199.
[13]高振,翟衡,孙鲁龙,等.基于温度-伤害度关系分析酿酒葡萄根系及芽抗寒性[J].应用生态学报,2014,25(4):983-990.
[14]刘杜玲,张博勇,彭少兵,等.基于早时核桃不同品种叶片组织结构的抗寒性划分[J].果树学报,2012,29(2):205-211.
[15]高京草,王长柱,王进国,等.枣树抗寒性测定方法研究[J].西北林学院学报,2011,26(5):72-75.
[16]王爱芳,张钢,魏士春,等.不同发育时期樟子松(Pinus sylvestris L. var. mongolica Litv.)的电阻抗参数与抗寒性的关系[J].生态学报,2008,28(11):5741-5749.
[17]邹琦.植物生理学实验指导[M].北京:中国农业出版社,2000.
[18]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006:144-148.
[19]Theocharis A,Clément C,Barka E A,et al. Physiological and molecular changes in plants grown at low temperatures[J]. Planta,2012,235(6):1091-1091.
[20]史清华,高建设,王军,等. 5个杨树无性系抗寒性的测定与评价[J].西北植物学报,2003,23(11):1937-1941.
[21]张文娥,王飞,潘学军.应用隶属函数法综合评价葡萄种间抗寒性[J].果树学报,2007,24(6):849-853.
[22]Repo T,Zhang G,Ryypp9 A,et al. The electrical impedance spectroscopy of Scots pine(Pinus sylvestris L.)Shoots in relation to cold acclimation[J]. Journal of Experimental Botany,2000,51(353):2095-2107.
[23]Nicese F P,Mancuso S,Masi E,et al. Comparing fractal analysis,electrical impendence and electrolyte leakage for the assessment of cold tolerance in Callistemon and Grevillea spp.[J]. Journal of Horticultural Science&Biotechnology,2004,79(4):627-632.
[24]Jan N,Andrabi K. Cold resistance in plants:A mystery unresolved[J]. Electronic Journal of Biotechnology,2009,12(3):1-15.
[25]Sakai A. Freezing resistance of ornamental trees and shrubs[J].Journal of American for Horticultural Science,1982,107(4):572-581.
[26]玉苏甫·阿不力提甫,阿依古丽·铁木儿,帕提曼·阿布都热合曼.利用隶属函数法综合评价梨砧木抗寒性[J].中国农业大学学报,2014,19(3):121-129.
[27]Castonguay Y,Bertrand A,Michaud R,et al. Cold-induced biochemical and molecular changes in populations selectively improved for freezing tolerance[J]. Crop Science,2011,51(5):2132-2144.