健脾清热活血法对萎缩性胃炎大鼠炎症通路因子的影响
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摘要
目的本研究旨在探讨健脾清热活血法对萎缩性胃炎(CAG)大鼠相关炎症通路因子的影响。方法将CAG大鼠随机分为6组:空白组、模型组、胃萎清高、中、低剂量组及叶酸组。以N-甲基-N′-硝基-N′-亚硝基胍(MNNG)合饥饱失常法造CAG大鼠模型,观察各组大鼠NF-κB炎症通路各因子的变化。结果胃萎清可在一定程度上抑制TNF-α的表达,对炎症的发生起到一定抑制作用。模型组IKK蛋白表达水平明显高于胃萎清高、中、低剂量组及空白组。胃萎清高、中、低剂量组IKK的表达水平与空白组相当,提示胃萎清能在一定程度上抑制炎症因子的表达,从而阻断炎症的发生发展过程。胃萎清高剂量组可下调p50蛋白的表达水平。结论胃萎清能使NF-κB炎症通路的致炎因子TNF-α、IKK、p50的表达水平下降从而起到控制CAG的发展。
Objective The aim of this study is to investigate effects of the Chinese medicine prescription of invigorating the spleen and clearing heat and activating blood on the inflammatory pathway factors in rats with chronic atrophic gastritis(CAG). Methods CAG rats were randomly divided into 6 groups: control group,model group, Weiweiqing high dose, middle dose and low dose groups, and folic acid group. The CAG rat model was made with N-methyl-N′-nitro-N′-nitroguanidine(MNNG) and hunger satiety method, and the changes of various factors in the inflammatory pathway of NF-κB in rats were observed in each group.Results Weiweiqing could inhibit the expression of TNF-α to a certain extent and inhibit the occurrence of inflammation. The expression level of IKK protein in model group was significantly higher than that of Weiweiqing high, medium and low dose groups as well as blank group. The expression levels of IKK protein in control group corresponded with Weiweiqing group. Weiweiqing could inhibit the inflammatory factors in a certain extent, thereby blocking the development of inflammation. The expression of P50 protein was downregulated in the high dose group. Conclusion Weiweiqing can reduce the expression levels of inflammatory cytokines TNF-α, IKK and P50 in the inflammatory pathway of NF-κB, thereby controlling the development of CAG.
Objective The aim of this study is to investigate effects of the Chinese medicine prescription of invigorating the spleen and clearing heat and activating blood on the inflammatory pathway factors in rats with chronic atrophic gastritis(CAG). Methods CAG rats were randomly divided into 6 groups: control group,model group, Weiweiqing high dose, middle dose and low dose groups, and folic acid group. The CAG rat model was made with N-methyl-N′-nitro-N′-nitroguanidine(MNNG) and hunger satiety method, and the changes of various factors in the inflammatory pathway of NF-κB in rats were observed in each group.Results Weiweiqing could inhibit the expression of TNF-α to a certain extent and inhibit the occurrence of inflammation. The expression level of IKK protein in model group was significantly higher than that of Weiweiqing high, medium and low dose groups as well as blank group. The expression levels of IKK protein in control group corresponded with Weiweiqing group. Weiweiqing could inhibit the inflammatory factors in a certain extent, thereby blocking the development of inflammation. The expression of P50 protein was downregulated in the high dose group. Conclusion Weiweiqing can reduce the expression levels of inflammatory cytokines TNF-α, IKK and P50 in the inflammatory pathway of NF-κB, thereby controlling the development of CAG.
引文
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[3]安康.TGEV感染PK-15细胞的蛋白质组学及诱导炎症反应的分子机制[D].华中农业大学,2015.
[4]张桂贤,刘洪斌,刘大卫.非可控性炎症与肿瘤相关性研究[J].中国中西医结合外科杂志,2015,21(2):197-201.
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[6]张德忠,江伟春,刘泽洪,等.肿瘤坏死因子α-308基因多态性与胃癌患者血清胃蛋白酶原的关系[J].中华临床实验室管理电子杂志,2015,3(4):249-252.
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