外源NO处理对新疆红提葡萄抗氧化酶活性的影响
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摘要
为了深入探究我国新疆地区的特产红提葡萄的贮藏性能,在整个检测过程中,借助外NO技术来对红提葡萄进行处理,对其所含的抗氧化酶进行活性检测,评估其贮藏性能。采用不同浓度硝普钠(SNP)(外源NO供体)溶液处理红提葡萄,封闭浸泡2 h,然后将其放置阴凉处,经过一定贮藏期,取样测定抗氧化酶抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD) 4种酶的活性,并将之与对照组比较。结果显示,用浓度为0.05 mmol/L SNP密闭浸泡2 h,贮藏60 d左右,贮藏期间检测得红提葡萄中APX、CAT、POD、SOD活性均比对照组高。这说明适宜浓度外源NO处理能够有效延长红提葡萄的贮藏期,延缓衰老进程,对红提葡萄的保鲜有较好的促进作用;主要是由于浓度适宜的外源NO溶液,可以在一定程度上极大地提升红提葡萄中抗氧化酶的活性,进而能够有效延缓红提葡萄的腐败时间,起到长时间保鲜贮藏的作用,这对相关水果产品的保存途径提供了一定的方向以及理论框架。
The main purpose of this exam is further to explore the specialties of China's Xinjiang region-red grape storage performance, among the whole testing process, mainly with the help of exogenous NO technology to red grape processing, and to measure the activity of antioxidant enzymes of red grape and then evaluating its storage performance. With different concentrations of sodium nitroprusside(SNP)(exogenous NO donor) solution of red grape, soak them closed for 2 h, and then place the shade, after determination of antioxidant enzyme ascorbate peroxidase(APX) sampling a certain storage period, catalase(CAT), peroxidase(POD) and superoxide dismutase(SOD) four enzyme activity, and then compared with the control group. The result shows that with the concentration of 0.05 mmol/L SNP closed for 2 h storage, 60 d, detecting the activity during storage in red grape APX, CAT, POD and SOD is higher than the control group. This shows that the suitable concentration of the storage period of exogenous NO treatment can effectively extend the grape, delay the aging process, and make the red grape in a very long time to keep fresh and have red grape preservation effect. The main cause is that the appropriate concentration of exogenous NO solution, to a certain extent, can greatly enhance the activity of antioxidant enzymes in red grape, which can effectively delay the corruption of red grape, and have the effect of preservation storage for a long time. The related fruit products preservation approach provides a certain direction, and theoretical framework.
The main purpose of this exam is further to explore the specialties of China's Xinjiang region-red grape storage performance, among the whole testing process, mainly with the help of exogenous NO technology to red grape processing, and to measure the activity of antioxidant enzymes of red grape and then evaluating its storage performance. With different concentrations of sodium nitroprusside(SNP)(exogenous NO donor) solution of red grape, soak them closed for 2 h, and then place the shade, after determination of antioxidant enzyme ascorbate peroxidase(APX) sampling a certain storage period, catalase(CAT), peroxidase(POD) and superoxide dismutase(SOD) four enzyme activity, and then compared with the control group. The result shows that with the concentration of 0.05 mmol/L SNP closed for 2 h storage, 60 d, detecting the activity during storage in red grape APX, CAT, POD and SOD is higher than the control group. This shows that the suitable concentration of the storage period of exogenous NO treatment can effectively extend the grape, delay the aging process, and make the red grape in a very long time to keep fresh and have red grape preservation effect. The main cause is that the appropriate concentration of exogenous NO solution, to a certain extent, can greatly enhance the activity of antioxidant enzymes in red grape, which can effectively delay the corruption of red grape, and have the effect of preservation storage for a long time. The related fruit products preservation approach provides a certain direction, and theoretical framework.
引文
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[13]屠荫华,惠伟,李彩香,等.硝普钠(SNP)对蒜苔保鲜效果的影响[J].食品工业科技,2008,29(10):244-247.
[14]高青海,吴燕,陆晓民,等.外源NO对菜豆荚采后保鲜效果的影响[J].食品与发酵工业,2009,35(3):191-194.
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[16]CAKMAK I,MARSCHNER H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,aseorbate peroxidase,and glutathione reducatse in bean leaves[J].Plant Physiology,1992(98):1222-1227.
[17]刘孟臣.一氧化氮处理提高肥城桃和猕猴桃果实贮藏性能生理机制的研究[D].泰安:山东农业大学,2007:30-32.
[18]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2001:164-167.
[19]曹建康,姜微波,赵玉梅,等.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007:29-105.
[20]邱贺撼,曾宪锋.广东产苋科六种野菜中硝酸盐、亚硝酸盐及VC的含量[J].食品科学,2004,25(11):250-251.
[21]朱树华,周杰,束怀瑞.植物中一氧化氮与园艺产品的成熟和衰老[J].植物生理学通讯,2004,40(6):733-740.
[22]王文举,张亚红,平吉成.外源抗氧化剂对高温胁迫下红地球葡萄果皮组织CAT与APX活性的影响[J].农业科学研究,2010,31(2):21-23.
[23]赵红梅,胡松竹,常勇.刨花楠扦插繁殖中POD、CAT的测定[J].江西林业科技,2010(1):1-2.
[24]邓茳明,熊格生,袁小玲,等.棉花不同耐高温品系的SOD、POD、CAT活性和MDA含量差异及其对盛花期高温胁迫的响应[J].棉花学报,2010,22(3):242-247.
[2]许玲,徐灵芝,张维一,等.葡萄采后病原菌种类及发病规律研究[J].新疆农业大学学报,1995(2):31-35.
[3]廖康.新疆葡萄生产现状和发展方向[J].新疆农业大学学报,2002,25(4):54-56.
[4]任小林,张少颖,于建娜.一氧化氮与植物成熟衰老的关系[J].西北植物学报,2004,24(1):167-171.
[5]CHEESEMAN K,SLATER T.An introduction to free radical biochemistry[J].British Medical Bulletin,1993,49(3):481-493.
[6]PRYOR W A.The role of free radical reactions in biological systems[J].Free Radicals in Biology,1976(1):1-49.
[7]方允中,李文杰.自由基和酶[M].北京:科学技术出版社,1989.
[8]关军锋,束怀瑞,黄天栋.苹果果实衰老与膜脂过氧化作用[J].河北农业大学学报,1991,14(1):50-53.
[9]DUAN X W,SU X G,YOU Y L,et al.Effect of nitric oxide on pericarp browning of harvested longan fruit in relation to phenolic metabolism[J].Food Chemistry,2007(104):571-576.
[10]王文举,王振平.亚硒酸钠对红葡萄耐热性及果实品质的影响[J].北方园艺,2012(24):47-49.
[11]张宪政,陈凤玉.植物生理学[M].长春:吉林科学出版社,1996.
[12]陈海荣.一氧化氮对采后青梗白菜处理的安全性评价及其对活性氧介导的衰老调控研究[D].北京:中国农业大学,2007:35-42.
[13]屠荫华,惠伟,李彩香,等.硝普钠(SNP)对蒜苔保鲜效果的影响[J].食品工业科技,2008,29(10):244-247.
[14]高青海,吴燕,陆晓民,等.外源NO对菜豆荚采后保鲜效果的影响[J].食品与发酵工业,2009,35(3):191-194.
[15]NAKANO Y,ASADA K.Hydrogen peroxide is scavenged by aseorbate-specific peroxidase in spinach chloroplasts[J].Plant Cell Physiology,1981(22):867-880.
[16]CAKMAK I,MARSCHNER H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,aseorbate peroxidase,and glutathione reducatse in bean leaves[J].Plant Physiology,1992(98):1222-1227.
[17]刘孟臣.一氧化氮处理提高肥城桃和猕猴桃果实贮藏性能生理机制的研究[D].泰安:山东农业大学,2007:30-32.
[18]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2001:164-167.
[19]曹建康,姜微波,赵玉梅,等.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007:29-105.
[20]邱贺撼,曾宪锋.广东产苋科六种野菜中硝酸盐、亚硝酸盐及VC的含量[J].食品科学,2004,25(11):250-251.
[21]朱树华,周杰,束怀瑞.植物中一氧化氮与园艺产品的成熟和衰老[J].植物生理学通讯,2004,40(6):733-740.
[22]王文举,张亚红,平吉成.外源抗氧化剂对高温胁迫下红地球葡萄果皮组织CAT与APX活性的影响[J].农业科学研究,2010,31(2):21-23.
[23]赵红梅,胡松竹,常勇.刨花楠扦插繁殖中POD、CAT的测定[J].江西林业科技,2010(1):1-2.
[24]邓茳明,熊格生,袁小玲,等.棉花不同耐高温品系的SOD、POD、CAT活性和MDA含量差异及其对盛花期高温胁迫的响应[J].棉花学报,2010,22(3):242-247.