LIM矿化蛋白1在大鼠牙髓损伤修复中的时空表达
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摘要
目的 :探讨LIM矿化蛋白1(LIM mineralization protein 1,LMP-1)在大鼠磨牙牙髓损伤修复中的表达。方法 :建立大鼠磨牙牙髓损伤修复动物模型,用免疫组织化学染色方法观察LMP-1在大鼠牙髓损伤修复中的表达,并用Image-Pro Plus 6.0图像分析软件及单因素方差分析比较各组间的差异。结果 :在正常大鼠牙髓组织中LMP-1表达阴性;在大鼠牙髓损伤后1 d,LMP-1表达于成牙本质细胞及部分牙髓成纤维细胞;术后3 d,LMP-1表达于坏死牙髓下方的细胞增殖层;术后7 d,LMP-1显著表达于增殖活跃的牙髓细胞及部分成牙本质细胞中。结论:LMP-1在牙髓损伤修复过程中呈时空特异性表达,可能参与成牙本质细胞及牙髓细胞的增殖、分化及修复性牙本质的形成。
PURPOSE: To investigate the expression and localization of LIM mineralization protein 1(LMP-1) during rat pulp injury and reparation. METHODS: Dental pulp injury models were established in maxillary first molars on one side of 12 Wistar rats, the isonym healthy teeth on the opposite side were used as the controls. Immunohistochemistry technique was used to observe the expression of LMP-1 at 1, 3 and 7 day after pulp injury. The results of staining were analyzed by Image-Pro Plus 6.0 and SPSS 17.0 software package. RESULTS: LMP-1 was not detected in normal rat dental pulp. Positive staining of LMP-1 was found in odontoblasts and some dental pulp cells at 1 day after pulp injury.The expression of LMP-1 was converged at cell proliferation zone under the injury site at 3 and 7 day after injury.CONCLUSIONS: LMP-1 might play a role in the dental pulp cell proliferation and differentiation and reparative dentin formation during pulp injury and reparation.
PURPOSE: To investigate the expression and localization of LIM mineralization protein 1(LMP-1) during rat pulp injury and reparation. METHODS: Dental pulp injury models were established in maxillary first molars on one side of 12 Wistar rats, the isonym healthy teeth on the opposite side were used as the controls. Immunohistochemistry technique was used to observe the expression of LMP-1 at 1, 3 and 7 day after pulp injury. The results of staining were analyzed by Image-Pro Plus 6.0 and SPSS 17.0 software package. RESULTS: LMP-1 was not detected in normal rat dental pulp. Positive staining of LMP-1 was found in odontoblasts and some dental pulp cells at 1 day after pulp injury.The expression of LMP-1 was converged at cell proliferation zone under the injury site at 3 and 7 day after injury.CONCLUSIONS: LMP-1 might play a role in the dental pulp cell proliferation and differentiation and reparative dentin formation during pulp injury and reparation.
引文
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[7]Wang X,Zhang Q,Chen Z,et al.Immunohistochemistry localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathological conditions[J].J Endod,2008,34(2):143-147.
[8]Fang P,Wang X,Zhang L,et al.Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development[J].J Mol Histol,2010,41(4-5):199-203.
[9]Sloan AJ,Smith AJ.Stimulation of the dentine-pulp complex of rat incisor teeth by transforming growth factor-beta isoforms 1-3in vitro[J].Arch Oral Biol,1999,44(2):149-156.
[10]Sloan AJ,Rutherford RB,Smith AJ.Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro[J].Arch Oral Biol,2000,45(2):173-177.
[11]Liu H,Bargouti M,Zughaier S,et al.Osteoinductive LIM mineralizationprotein-1 suppresses activation of NF-κB and selectively regulates MAPK pathways in pre-osteoclasts[J].Bone,2010,46(5):1328-1335.
[12]Sangadala S,Boden SD,Viggeswarapu M,et al.LIM mineralization protein-1 potentiates bone morphogenetic protein responsiveness via a novel interaction with Smurf1 resulting in decreased ubiquitination of Smads[J].J Biol Chem,2006,281(25):17212-17219.
[13]Wang X,Cui F,Madhu V,et al.Combined VEGF and LMP-1delivery enhances osteoprogenitor cell differentiation and ectopic bone formation[J].Growth Factors,2011,29(l):36-48.
[14]Liu H,Huang L,Zhang Z,et al.LIM mineralization protein-1inhibits the malignant phenotypes of human osteosarcoma cells[J].Int J Mol Sci,2014,15(4):7037-7048.
[2]Viggeswarapu M,Boden SD,Liu Y,et al.Adenoviral delivery of LIM mineralization protein-1 induces new-bone formation in vitro and in vivo[J].J Bone Joint Surg Am,2001,83-A(3):364-367.
[3]Kim HS,Viggeswarapu M,Boden SD,et al.Overcoming the immune response to permit ex vivo gene therapy for spine fusion with human type 5 adenoviral delivery of the LIM mineralization protein-1 c DNA[J].Spine(Phila Pa 1976),2003,28(3):219-226.
[4]Bunger MH,Langdahl BL,Andersen T,et al.Semiquantitative m RNA measurements of osteoinductive growth factors in human iliac-crest bone:expression of LMP splice variants in human bone[J].Calcif Tissue Int,2003,73(5):446-454.
[5]Minamide A,Boden SD,Viggeswarapu M,et al.Mechanism of bone formation with gene transfer of the c DNA encoding for the intracellular protein LMP-1[J].J Bone Joint Surg Am,2003,85-A(6):1030-1039.
[6]Zhang Q,Wang X,Chen Z,et al.Semi-quantitative RT-PCR analysis of LIM mineralization protein 1 and its associate molecules in cultured human dental pulp cells[J].Arch Oral Biol,2007,52(8):720-726.
[7]Wang X,Zhang Q,Chen Z,et al.Immunohistochemistry localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathological conditions[J].J Endod,2008,34(2):143-147.
[8]Fang P,Wang X,Zhang L,et al.Immunohistochemical localization of LIM mineralization protein 1 during mouse molar development[J].J Mol Histol,2010,41(4-5):199-203.
[9]Sloan AJ,Smith AJ.Stimulation of the dentine-pulp complex of rat incisor teeth by transforming growth factor-beta isoforms 1-3in vitro[J].Arch Oral Biol,1999,44(2):149-156.
[10]Sloan AJ,Rutherford RB,Smith AJ.Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro[J].Arch Oral Biol,2000,45(2):173-177.
[11]Liu H,Bargouti M,Zughaier S,et al.Osteoinductive LIM mineralizationprotein-1 suppresses activation of NF-κB and selectively regulates MAPK pathways in pre-osteoclasts[J].Bone,2010,46(5):1328-1335.
[12]Sangadala S,Boden SD,Viggeswarapu M,et al.LIM mineralization protein-1 potentiates bone morphogenetic protein responsiveness via a novel interaction with Smurf1 resulting in decreased ubiquitination of Smads[J].J Biol Chem,2006,281(25):17212-17219.
[13]Wang X,Cui F,Madhu V,et al.Combined VEGF and LMP-1delivery enhances osteoprogenitor cell differentiation and ectopic bone formation[J].Growth Factors,2011,29(l):36-48.
[14]Liu H,Huang L,Zhang Z,et al.LIM mineralization protein-1inhibits the malignant phenotypes of human osteosarcoma cells[J].Int J Mol Sci,2014,15(4):7037-7048.