背景荧光猝灭-免疫层析定量检测尿纤维连接蛋白及膀胱癌辅助诊断的应用
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摘要
目的:采用基于背景荧光猝灭-免疫层析技术研制的尿纤维连接蛋白(Fn)定量检测卡,建立一种快速、简单、无创的定量检测膀胱癌肿瘤标记物的方法。方法:使用Fn定量检测卡,分别检测尿液样本膀胱癌组50例、健康人组100例、其他部位肿瘤组50例和良性泌尿系统疾病组14例,利用统计分析确定出膀胱癌患者cutoff值、灵敏度、特异度,对检测结果进行LSD-t多重比较显著性分析。结果:在Fn检测结果统计分析中,膀胱癌患者组与其他各组Fn值比较差异均有统计学意义(P<0.05),而其他各组之间两两比较差异均无统计学意义(P>0.05),ROC-Fn曲线中得出cut-off为324.05ng/ml,灵敏度为70.0%,特异度为99.9%。结论:Fn检测卡能定量检测尿液中Fn含量,有较高的专属性,检测速度快,有望成为膀胱癌辅助诊疗的新方法。
Objective:Based on background fluorescence quenching--immunochromatography technology and the principle of antigen-antibody reaction,the paper developed a quantitative detection card for urinary fibronectin(Fn),and established a rapid,simple,non-invasive and highly accurate method for the quantitative detection of bladder cancer tumor markers.Method:Fn quantitative detection card was used to detect 50 urine samples in bladder cancer group,100 healthy people group,50 in other tumor group and 14 in benign urinary system diseases group,and statistical analysis was used to determine cut-off value,sensitivity and specificity of bladder cancer patients,LSD-t multiple significant analysis was performed on the test results.Result:In the statistical analysis of Fn test results,Fn values of bladder cancer patients group and other groups showed extremely significant difference(P<0.05),while no significant difference was found among other groups(P>0.05).Cut-off=324.05 ng/ml was obtained from the ROC Fn curve,with a sensitivity of 70.0%and a specificity of 99.9%.Conclusion:Fn detection card can quantitatively detect the content of Fn in urine,which has high specificity and fast detection speed.It is expected to become a new method for auxiliary diagnosis and treatment of bladder cancer.
Objective:Based on background fluorescence quenching--immunochromatography technology and the principle of antigen-antibody reaction,the paper developed a quantitative detection card for urinary fibronectin(Fn),and established a rapid,simple,non-invasive and highly accurate method for the quantitative detection of bladder cancer tumor markers.Method:Fn quantitative detection card was used to detect 50 urine samples in bladder cancer group,100 healthy people group,50 in other tumor group and 14 in benign urinary system diseases group,and statistical analysis was used to determine cut-off value,sensitivity and specificity of bladder cancer patients,LSD-t multiple significant analysis was performed on the test results.Result:In the statistical analysis of Fn test results,Fn values of bladder cancer patients group and other groups showed extremely significant difference(P<0.05),while no significant difference was found among other groups(P>0.05).Cut-off=324.05 ng/ml was obtained from the ROC Fn curve,with a sensitivity of 70.0%and a specificity of 99.9%.Conclusion:Fn detection card can quantitatively detect the content of Fn in urine,which has high specificity and fast detection speed.It is expected to become a new method for auxiliary diagnosis and treatment of bladder cancer.
引文
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7吴晓霞,周晓燕,李久彤,等.基于背景荧光猝灭-免疫层析法黄曲霉毒素B1检测卡的研制[J].分析试验室,2017,36(2):189-193.
8 Chen X J,Xu Y Y,Yu J S,et al.Antigen detection based on background fluorescence quenching immunochromatographic assay[J].Anal Chim Acta,2014,841:44-50.
9徐林发,汪素珍,王柏省.应用ROC曲线求解最佳切点的方法介绍[J].中国卫生统计,2011,28(6):701-702.
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11孔令启,屈艳琳.尿液中LncRNA UCA1检测对膀胱癌诊断的Meta分析[J].临床泌尿外科杂志,2017,32(12):968-970.
12徐子程,蔡宏宙,喻彬,等.膀胱肿瘤相关抗原在中国人群膀胱尿路上皮癌诊断中的应用[J].临床泌尿外科杂志,2016,31(10):900-902.
2陈子坚,陈明坤,黄仲曦,等.纤维连接蛋白1在膀胱癌组织的表达及临床意义[J].中华实验外科杂志,2016,33(6):1444-1447.
3 Wang Y J.Antigen Detection Based on Background Fluorescence Quenching Immunochromatographic Assay[J].Anal Chim Acta,2014,7:23-26.
4 Wu X,Tian X,Xu L,et al.Determination of Aflatoxin M1and Chloramphenicol in Milk Based on Background Fluorescence Quenching Immunochromatographic Assay[J].Bio Med Res Int,2017,2017:8649314.
5 Zhang W,Chen J L,Wang Y W,et al.Development of a quantitative detection card for Hear-Type Fatty AciBinding Protein based on background fluorescence quenching immune chromatography[J].J Med Biochem,2018,37:1-9.
6陈俊蕾,张唯,李新霞,等.背景荧光猝灭-免疫层析法检测C-反应蛋白[J].高等学校化学学报,2018,39(1):41-47.
7吴晓霞,周晓燕,李久彤,等.基于背景荧光猝灭-免疫层析法黄曲霉毒素B1检测卡的研制[J].分析试验室,2017,36(2):189-193.
8 Chen X J,Xu Y Y,Yu J S,et al.Antigen detection based on background fluorescence quenching immunochromatographic assay[J].Anal Chim Acta,2014,841:44-50.
9徐林发,汪素珍,王柏省.应用ROC曲线求解最佳切点的方法介绍[J].中国卫生统计,2011,28(6):701-702.
10刘妍,王红宇,田娇妮,等.无金标准条件下含协变量的ROC曲线估计方法[J].中国卫生统计,2012,29(3):321-324.
11孔令启,屈艳琳.尿液中LncRNA UCA1检测对膀胱癌诊断的Meta分析[J].临床泌尿外科杂志,2017,32(12):968-970.
12徐子程,蔡宏宙,喻彬,等.膀胱肿瘤相关抗原在中国人群膀胱尿路上皮癌诊断中的应用[J].临床泌尿外科杂志,2016,31(10):900-902.