长江刀鲚选育和野生群体遗传多样性的微卫星分析
|
摘要
利用微卫星标记技术,对长江刀鲚野生群体(YS)与3个连续选育世代群体(F_1、F_2、F_3)间的遗传多样性进行了分析。12对多态性微卫星引物总共检测到等位基因82个,平均每对引物获得等位基因6. 83个。4个群体的平均有效等位基因数(Ne)、平均观测杂合度(Ho)、平均期望杂合度(He)以及平均多态信息含量(PIC)分别为3. 47～4. 10、0. 400 0～0. 516 7、0. 684 4～0. 738 1和0. 646 4～0. 701 2。F_1、F_2、F_3之间的遗传分化微弱(FST<0. 05),并与YS具有中等程度的遗传分化(0. 05
引文
[1]施永海,张根玉,张海明,等.配合饲料和活饵料喂养刀鲚肌肉营养品质分析与比较[J].动物营养学报,2014,26(2):427-436.
[2]邓平平,施永海,张根玉,等.磁珠富集法分离刀鲚微卫星标记[J].生物技术通报,2014(6):145-149.
[3]施永海,张根玉,张海明,等.刀鲚的全人工繁殖及胚胎发育[J].上海海洋大学学报,2015,24(1):36-43.
[4]袁传宓.长江中下游刀鲚资源和种群组成变动状况及其原因[J].动物学杂志,1988,23(3):12-15.
[5]魏广莲,徐钢春,顾若波,等.刀鲚的生物学及人工养殖研究进展[J].长江大学学报(自然科学版):农学卷,2013,9(7):31-35.
[6]杨铭,于洋,张晓军,等.基于转录组数据的凡纳滨对虾微卫星标记开发[J].海洋科学,2017,41(2):96-102.
[7]白翠翠,柳淑芳,庄志猛.虾虎鱼科(Gobiidae)基因组微卫星DNA的分布特征[J].渔业科学进展,2016,37(5):9-15.
[8]管奥,毋玉婷,陈宇,等.曼氏无针乌贼转录组微卫星特征分析[J].渔业科学进展,2018,39(3):144-151.
[9]张燕萍,贺刚,王生,等.基于线粒体DNA D-loop序列分析刀鲚与短颌鲚的遗传多样性[J].江苏农业科学,2017,45(20):59-64.
[10]CHENG Q Q,ZHANG Q Y,MA CH Y,et al. Genetic structure and differentiation of four lake populations of Coilia ectenes(Clupeiformes:Engraulidae)based on mt DNA control region sequences[J]. Biochemical Systematics&Ecology,2011,39(4/5/6):544-552.
[11]魏广莲,徐钢春,顾若波,等.基于mt DNA Cytb序列分析养殖与野生刀鲚群体的遗传多样性[J].江西农业大学学报,2012,34(6):1216-1221.
[12]MA CH Y,CHENG Q Q,ZHANG Q Y. Genetic variation of Coilia ectenes(Clupeiformes:Engraulidae)revealed by the complete cytochrome b sequences of mitochondrial DNA[J]. Journal of Experimental Marine Biology&Ecology,2010,385(1):14-19.
[13]马春艳,刘敏,马凌波,等.长江口刀鲚遗传多样性的随机扩增多态DNA(RAPD)分析[J].海洋水产研究,2004,25(5):19-24.
[14]葛家春,曹廷,陈婵娟,等.利用扩增片断长度多态性技术分析长江刀鲚的遗传多样性[J].南京大学学报(自然科学版),2008,44(3):332-338.
[15]RONG X J,XU Y J,WANG Q Y,et al. Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes[J]. Genetics&Molecular Research,2013,12(4):6011-6017.
[16]MA CH Y,CHENG Q Q,ZHANG Q Y. Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale,1905(Clupeiformes:Engraulidae)and cross-species amplification in Coilia mystus Linnaeus,1758[J]. Environmental Biology of Fishes,2011,91(2):243-249.
[17]CHEN F,ZENG L Y,CHENG Q Q. Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes(Clupeiformes:Engraulidae)and cross-species amplification in two closely related taxa[J]. Journal of Genetics,2012,91(1):1-7.
[18]PEAKALL R,SMOUSE P E. GENALEX 6:genetic analysis in Excel. Population genetic software for teaching and research[J].Molecular Ecology Resources,2006,6(1):288-295.
[19]NEI M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics,1978,89(3):583-590.
[20]SCHNEIDER S,ROESSLI D,EXCO E L. Arlequin version2000:a software for population genetics data analysis[M].Switzerland:Genetics and biometry laboratory,University of Geneva,2000.
[21]于爱清,施永海,张海明,等.暗纹东方鲀养殖群体和野生群体遗传多样性的ISSR分析[J].水产科技情报,2018,45(1):1-5.
[22]NORRIS A T,BRADLEY D G,CUNNINGHAM E P. Microsatellite genetic variation between and within farmed and wild Atlantic salmon(Salmo salar)populations[J]. Aquaculture,1999,180(3):247-264.
[2]邓平平,施永海,张根玉,等.磁珠富集法分离刀鲚微卫星标记[J].生物技术通报,2014(6):145-149.
[3]施永海,张根玉,张海明,等.刀鲚的全人工繁殖及胚胎发育[J].上海海洋大学学报,2015,24(1):36-43.
[4]袁传宓.长江中下游刀鲚资源和种群组成变动状况及其原因[J].动物学杂志,1988,23(3):12-15.
[5]魏广莲,徐钢春,顾若波,等.刀鲚的生物学及人工养殖研究进展[J].长江大学学报(自然科学版):农学卷,2013,9(7):31-35.
[6]杨铭,于洋,张晓军,等.基于转录组数据的凡纳滨对虾微卫星标记开发[J].海洋科学,2017,41(2):96-102.
[7]白翠翠,柳淑芳,庄志猛.虾虎鱼科(Gobiidae)基因组微卫星DNA的分布特征[J].渔业科学进展,2016,37(5):9-15.
[8]管奥,毋玉婷,陈宇,等.曼氏无针乌贼转录组微卫星特征分析[J].渔业科学进展,2018,39(3):144-151.
[9]张燕萍,贺刚,王生,等.基于线粒体DNA D-loop序列分析刀鲚与短颌鲚的遗传多样性[J].江苏农业科学,2017,45(20):59-64.
[10]CHENG Q Q,ZHANG Q Y,MA CH Y,et al. Genetic structure and differentiation of four lake populations of Coilia ectenes(Clupeiformes:Engraulidae)based on mt DNA control region sequences[J]. Biochemical Systematics&Ecology,2011,39(4/5/6):544-552.
[11]魏广莲,徐钢春,顾若波,等.基于mt DNA Cytb序列分析养殖与野生刀鲚群体的遗传多样性[J].江西农业大学学报,2012,34(6):1216-1221.
[12]MA CH Y,CHENG Q Q,ZHANG Q Y. Genetic variation of Coilia ectenes(Clupeiformes:Engraulidae)revealed by the complete cytochrome b sequences of mitochondrial DNA[J]. Journal of Experimental Marine Biology&Ecology,2010,385(1):14-19.
[13]马春艳,刘敏,马凌波,等.长江口刀鲚遗传多样性的随机扩增多态DNA(RAPD)分析[J].海洋水产研究,2004,25(5):19-24.
[14]葛家春,曹廷,陈婵娟,等.利用扩增片断长度多态性技术分析长江刀鲚的遗传多样性[J].南京大学学报(自然科学版),2008,44(3):332-338.
[15]RONG X J,XU Y J,WANG Q Y,et al. Isolation and characterization of polymorphic microsatellite markers from Coilia ectenes[J]. Genetics&Molecular Research,2013,12(4):6011-6017.
[16]MA CH Y,CHENG Q Q,ZHANG Q Y. Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale,1905(Clupeiformes:Engraulidae)and cross-species amplification in Coilia mystus Linnaeus,1758[J]. Environmental Biology of Fishes,2011,91(2):243-249.
[17]CHEN F,ZENG L Y,CHENG Q Q. Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes(Clupeiformes:Engraulidae)and cross-species amplification in two closely related taxa[J]. Journal of Genetics,2012,91(1):1-7.
[18]PEAKALL R,SMOUSE P E. GENALEX 6:genetic analysis in Excel. Population genetic software for teaching and research[J].Molecular Ecology Resources,2006,6(1):288-295.
[19]NEI M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics,1978,89(3):583-590.
[20]SCHNEIDER S,ROESSLI D,EXCO E L. Arlequin version2000:a software for population genetics data analysis[M].Switzerland:Genetics and biometry laboratory,University of Geneva,2000.
[21]于爱清,施永海,张海明,等.暗纹东方鲀养殖群体和野生群体遗传多样性的ISSR分析[J].水产科技情报,2018,45(1):1-5.
[22]NORRIS A T,BRADLEY D G,CUNNINGHAM E P. Microsatellite genetic variation between and within farmed and wild Atlantic salmon(Salmo salar)populations[J]. Aquaculture,1999,180(3):247-264.