去除单细胞蛋白中核酸方法的研究
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摘要
酵母单细胞蛋白和甲烷单细胞蛋白在饲料中应用广泛,解决了我国饲料应用不足的问题。单细胞蛋白中含有丰富的营养物质,但其中核酸含量过高,影响动物的健康,需要将其去除。去除单细胞蛋白有多种方法,文章介绍和归纳目前文献报道中从单细胞蛋白中除去和提取核酸的方法,包括超声波破碎法、菌体自溶法、浓盐法、浓盐与超声波破碎结合法、高压脉冲电场破壁法。前人使用方法的核酸提取量分别为46.47%、50.20%、78.90%、45.19%、25.40%。对这些方法进行总结与评估,希望对去除单细胞蛋白中核酸的研究有所帮助,为单细胞蛋白在我国饲料行业更好的发展提供参考。
At present, yeast single cell protein and methane single cell protein are widely used in feed sector, which solved the problem of lack of feed in our country. Single cell protein is rich in nutrients, but the nucleic acid content is exorbitant,it affects the health of animals, which is need to be removed. There are different ways to get rid of nucleic in single cell protein. This paper introduces and summarizes various methods to wipe nucleic acid in single cell protein, including ultrasonic broken, somatic cell method, concentrated salt method, and combination of concentrated salt and ultrasonic crushing method, high-voltage pulsed electric field wall breaking method. The amount of nucleic acid extracted by previous methods were 46.47%,50.20%,78.90%,45.19% and 25.40%, respectively. These methods are summarized and evaluated in the hope of helping the research on the removal of nucleic acids from single-cell proteins and the better development of single-cell proteins in China's feed industry.
At present, yeast single cell protein and methane single cell protein are widely used in feed sector, which solved the problem of lack of feed in our country. Single cell protein is rich in nutrients, but the nucleic acid content is exorbitant,it affects the health of animals, which is need to be removed. There are different ways to get rid of nucleic in single cell protein. This paper introduces and summarizes various methods to wipe nucleic acid in single cell protein, including ultrasonic broken, somatic cell method, concentrated salt method, and combination of concentrated salt and ultrasonic crushing method, high-voltage pulsed electric field wall breaking method. The amount of nucleic acid extracted by previous methods were 46.47%,50.20%,78.90%,45.19% and 25.40%, respectively. These methods are summarized and evaluated in the hope of helping the research on the removal of nucleic acids from single-cell proteins and the better development of single-cell proteins in China's feed industry.
引文
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[31]张若兵,杜钢,傅贤,等.采用高压脉冲电场技术的食品处理装置电极设备电化学腐蚀[J].高电压技术,2014,40(6):1889-1894.
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[34]刘铮.高压脉冲电场及超生场提取啤酒废酵母中蛋白质与核酸[D].无锡:江南大学,2007.
[2]孔祥霞,高婷婷,刘相春.单细胞蛋白的开发与利用[J].山东畜牧兽医,2016,37(9):28.
[3]Romarheim O H,Landsverk T,Myd Land L T,et al.Cell wall fractions from methylococcus capsulatus prevent soybean meal induced enteritis in Atlantic salmon(Salmosalar)[J].Aquaculture,2013,402/403:13-18.
[4]侯胜奎,胡慧艳,刘津,等.新型可再生动物蛋白饲料资源-昆虫粉的开发和利用[J].饲料博览,2017(12):35-38.
[5]Sara Cantera,Raul Munoz,Raquel Lebrero,et al.Technologies for the bioconversion of methane into more valuable products[J].Science Direct,2018,50:128-135.
[6]Sara Cantera,Raquel Lebrero,Lidia Sadornil,et al.Valorization of emissions into high-added-value products Assessing the production of ectione coupled withabatement[J].Journal of Environmental Management,2016,182:160-165.
[7]Sara Cantera,Raquel Lebrero,Suni Rodriguez,et al.Ectoine bio-milking in methanotrophs:A step further towards methane-based bio-refineries into high added-value products[J].Chemical Engineering Journal,2017,328:44-48.
[8]林惠颖,辛嘉英,李春雨,等.颗粒性单加氧酶分离纯化方法的研究进展[J].分子催化,2018,32(1):90-98.
[9]江梅,辛嘉英,邓晓萍,等.甲烷氧化菌生产高附加值产品的研究进展[J].石油化工,2018,47(4):395-400.
[10]Gamboa-Delgado J,Marquez-Reres J M.Potential of microbialderived nutrients for aquaculture development[J].Rev Aquac 2016.http://dx.Doi.org/10.1111/raq.12157.
[11]刘秀梅,刘艳新,石雅丽,等.高产单细胞蛋白诱变酿酒酵母的筛选[J].华夏酒报,2017,3(28):1-4.
[12]刘明美.蛋白饲料的开发利用现状[J].江西饲料,2012(6):17-19.
[13]梁蕊芳,徐龙,岳明强.细胞破碎技术应用研究进展[J].内蒙古农业科技,2013,1(55):113-114.
[14]Lee I,Han J I.Simultaneous treatment(cell disruption and lipid extraction)of wet microalgae using hydrodynamic cavitation for enhancing the lipid yield[J].Bioresource Technology,2015,186:246-251.
[15]Rakesh S,Dhar D W,Prasanna R,et al.Cell disruption methods for improving lipid extraction efficiency in unicellular microalgae[J].Engineering in Life Sciences,2015,15(4):443-447.
[16]曾剑华,杨杨,石彦国,等.适度破碎微藻细胞释放功能性蛋白的技术研究进展[J].食品工业科技,2018,17:319-334.
[17]敖存,郭敏明,郑旭霞,等.细胞破碎技术在红茶加工中的应用研究进展[J].杭州农业与科技,2015(3):19-21.
[18]韩彦爱,牟真.研磨法与冻融法测定湖库叶绿素a的方法探讨[J].中国水能及电气化,2018(2):18-20.
[19]王迪,曹方,栾静.超声波破碎法提取发酵性丝孢酵母胞内脂肪酶条件优化[J].大连工业大学学报,2012,31(6):405-408.
[20]贾韶千,李艳霞.超声波强化提取香椿叶总三萜的工艺研究[J].农产品加工,2016,4(7):28-31.
[21]刘轲.甲烷蛋白的制备及核酸去除[D].哈尔滨:哈尔滨商业大学,2014.
[22]李英,张晓锋,候英敏.超声波破碎发酵性丝孢酵母提取蛋白酶条件的研究[J].声学技术,2015,34(3):3-4.
[23]陈裕,张发宇,赵冰冰,等.冻融超声波联合破壁法提取巢湖蓝藻中藻蓝蛋白的研究[J].低碳世界,2015(6):17-18.
[24]凌秀梅,邱树毅,曲源,等.啤酒废酵母蛋白液的制取及其精制[J].中国酿造,2007,26(5):27-31.
[25]姚洪文,郭素格,范玉梅.酵母细胞破碎技术的研究[J].中国酿造,2015,24(4):32-34.
[26]孟国庆,王传宝,朱陶.啤酒废酵母细胞破壁方法的研究[J].中国果菜,2014(12):30-34.
[27]孙荣丹,刘莹,张洪林.浓盐法与稀碱法在啤酒废酵母中提取RNA的研究[J].氨基酸和生物资源,2006,28(3):76-78.
[28]聂琴,杨凡,易建华.酵母类单细胞蛋白原料在水产养殖中的应用[J].科学实验研究,2017,11(12):42-44.
[29]于彩霞,李勤.啤酒酵母工业化破壁方法的研究[J].工艺技术,2017(15):102-103.
[30]李艳,牟德华,杨翠竹.超声波与食盐法结合从啤酒酵母中提取核糖核酸[J].酿酒科技,2007(3):97-99.
[31]张若兵,杜钢,傅贤,等.采用高压脉冲电场技术的食品处理装置电极设备电化学腐蚀[J].高电压技术,2014,40(6):1889-1894.
[32]张若兵,傅贤,寇梅如.高压脉冲电场对小球藻破碎效果的影响[J].高电压技术,2016,42(8):2668-3674.
[33]周连宁,刘晓东,余麟,等.不同高压脉冲电场条件对小球藻破壁的影响研究[J].科学技术与工程,2016,16(1):47-51.
[34]刘铮.高压脉冲电场及超生场提取啤酒废酵母中蛋白质与核酸[D].无锡:江南大学,2007.