利用SSR分析华中、华东五大湖区野生菰的遗传结构与多样性
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摘要
为进一步了解我国野生菰资源的生存现状及演进历程,利用7对SSR分子标记对来自华中、华东5大湖区的26个野生菰居群的遗传多样性和遗传结构进行了分析。结果表明,在物种水平上,华中、华东野生菰具有较丰富的遗传多样性(PPB=92.19%、Ao=1.922、Ae=1.454、He=0.274、I=0.422);各野生菰居群间的遗传相似性系数和遗传距离分别介于0.5569~0.9981和0.0019~0.5854之间,居群间的遗传分化较显著(Gst=0.673)。26个野生菰居群的遗传结构可以分为3组,且各居群间不存在明显的亲缘地理关系。此外,进化分析结果显示,26个野生菰居群具有共同的祖先,在进化上分属两支。本研究结果深化了对我国野生菰资源遗传进化的认识,为野生菰资源开发利用及种质资源保护提供了理论依据。
In order to further understand the survival condition and evolution of Z. latifolia in China,seven SSR markers were used for genetic diversity and genetic structure analysis of 26 wild populations from the Middle-East China. The results of POPGENE analysis showed that there was rich genetic diversity among the wild species(PPB = 92. 19%,Ao =1. 922,Ae = 1. 454,He = 0. 274,I = 0. 422,Gst = 0. 673) and the genetic similarity coefficient and genetic distance were between 0. 5569 ~ 0. 9981 and 0. 0019 ~ 0. 5854,respectively. With the genetic structure analysis,26 populations were divided into three groups and there was no significant association between the phylogenetic relationship and the geographic locations in the Z. latifolia populations. The evolution analysis showed that 26 populations were separated from the same ancestor into two branches. This study deepens our understanding about the genetic evolution of Z.latifolia in the Middle-East China and provides certain theoretical basis for protection of the wild rice germplasm resource.
In order to further understand the survival condition and evolution of Z. latifolia in China,seven SSR markers were used for genetic diversity and genetic structure analysis of 26 wild populations from the Middle-East China. The results of POPGENE analysis showed that there was rich genetic diversity among the wild species(PPB = 92. 19%,Ao =1. 922,Ae = 1. 454,He = 0. 274,I = 0. 422,Gst = 0. 673) and the genetic similarity coefficient and genetic distance were between 0. 5569 ~ 0. 9981 and 0. 0019 ~ 0. 5854,respectively. With the genetic structure analysis,26 populations were divided into three groups and there was no significant association between the phylogenetic relationship and the geographic locations in the Z. latifolia populations. The evolution analysis showed that 26 populations were separated from the same ancestor into two branches. This study deepens our understanding about the genetic evolution of Z.latifolia in the Middle-East China and provides certain theoretical basis for protection of the wild rice germplasm resource.
引文
[1]陈守良,徐克学.菰属Zizania L.植物的分支分类研究[J].植物研究,1994,14(4):385-393
[2]翟成凯,孙桂菊.中国菰资源及其应用价值的研究[J].资源科学,2000,22(6):22-26
[3]Guo H B,Li S M,Peng J,Ke W D.Zizania latifolia Turcz.cultivated in China[J].Genetic Resources and Crop Evolution,2007,54(6):1211-1217
[4]官少飞,郎青,张本.鄱阳湖水生植被[J].水生生物学报,1987,11(1):9-21
[5]彭晓赟,赵运林,雷存喜,戴枚斌,易合成.菰对南洞庭湖湿地土壤中Cu、Sb、Cd、Pb的吸收与富集[J].中国农学通报,2009,(13):206-210
[6]Yu X,K9nig T,Zhang Q,Gao Y S.Nitrogen and phosphorus removal of locally adapted plant species used in constructed wetlands in China[J].Water Science and Technology,2012,66(4):695-703
[7]付硕章,柯文山,陈世俭.洪湖湿地野菰群落储碳、固碳功能研究[J].湖北大学学报(自然科学版),2013,35(3):393-396
[8]闫宁,王晓清,王志丹,张艳丽,薛惠民,郭得平.食用黑粉菌侵染对茭白植株抗氧化系统和叶绿素荧光的影响[J].生态学报,2013,33(5):1584-1593
[9]Przybylski R,Klensporfpawlik D,Anwar F,Rudzinska M.Lipid components of North American wild rice(Zizania palustris)[J].Journal of the American Oil Chemists'Society,2009,86(6):553-559
[10]Han S F,Zhang H,Zhai C K.Protective potentials of wild rice(Zizania latifolia(Griseb)Turcz)against obesity and lipotoxicity induced by a high-fat/cholesterol diet in rats[J].Food and Chemical Toxicology,2012,50(7):2263-2269
[11]Han S F,Zhang H,Qin L Q,Zhai C K.Effects of dietary carbohydrate replaced with wild rice(Zizania latifolia(Griseb)Turcz)on insulin resistance in rats fed with a high-fat/cholesterol diet[J].Nutrients,2013,5(2):552-564
[12]Lee S S,Baek Y S,Eun C S,Yu M H,Baek N I,Chung D K,Bang M H,Yang S A.Tricin derivatives as anti-inflammatory and anti-allergic constituents from the aerial part of Zizania latifolia[J].Bioscience Biotechnology and Biochemistry,2015,79(5):700-706
[13]吴国林.基于ISSR和SSR标记的鄱阳湖流域野生菰种质资源遗传多样性研究[D].南昌:江西农业大学,2014:29-30
[14]任绪瑞,刘艳玲,杨美,陈媛媛,王冬良,陈友根.菰SRAPPCR反应体系的优化与建立[J].热带作物学报,2014,35(2):299-306
[15]郑道君,云天海,张治礼,邓长智,谢良商.中国南瓜海南农家品种资源遗传多样性和亲缘关系研究[J].核农学报,2016,30(5):869-877
[16]戴智慧,龙骏,俞雷民,倪穗.宁波地区野生换锦花遗传多样性的ISSR分析[J].核农学报,2016,30(10):1898-1905
[17]Xu X W,Ke W D,Yu X P,Wen J,Ge S.A preliminary study on population genetic structure and phylogeography of the wild and cultivated Zizania latifolia(Poaceae)based on Adh1a sequences[J].Theoretical and Applied Genetics,2008,116(6):35-43
[18]Chen Y Y,Chu H J,Liu H,Liu Y L.Abundant genetic diversity of the wild rice Zizania latifolia in central China revealed by microsatellites[J].Annals of Applied Biology,2012,161(2):192-201
[19]Xu X W,Wu J W,Qi M X,Lu Q X,Lee P F,Lutz S,Ge S,Wen J.Comparative phylogeography of the wild-rice genus Zizania(Poaceae)in eastern Asia and North America[J].American Journal of Botany,2015,102(2):239-247
[20]王惠梅,吴国林,江绍琳,黄奇娜,奉保华,黄长干,江绍玫,吴建利.基于SSR和ISSR鄱阳湖流域野生菰(Zizania latifolia)资源的遗传多样性分析[J].植物遗传资源学报,2015,16(1):133-141
[21]苗培明,范玲,师维军,乌买尔江,徐利民,马君.棉花种质资源遗传多样性的TRAP分析[J].棉花学报,2009,21(5):420-426
[22]奉保华.水稻淡褐斑叶突变体lbsl1的鉴定、遗传分析与基因定位[D].北京:中国农业科学院,2012:27-28
[23]Richards C M,Reilley A,Touchell D,Antolin M F,Walterset C.Microsatellite primers for Texas wild rice(Zizania texana),and a preliminary test of the impact of cryogenic storage on allele frequency at these loci[J].Conservation Genetics,2004,5(6):853-859
[24]Quan Z,Pan L,K W D,Liu Y,Ding Y.Sixteen polymorphic microsatellite markers from Zizania latifolia Turcz.(Poaceae)[J].Molecular Ecology Resources,2009,9(3):887-889
[25]李晓红,张慧,王德元,张莉,邵剑文,张小平.我国特有植物青檀遗传结构的ISSR分析[J].生态学报,2013,33(16):4892-4901
[26]Yeh F C,Yang R,Boyle T.POPGENE Version 1.32.Microsoft windows-based freeware for population genetic analysis[EB/OL].(2016-10-10)[2017-03-01].https://sites.ualberta.ca/~fyeh/popgene.html
[27]Pritchard J K,Stephens M,Donnelly P.Inference of population structure using multilocus genotype data[J].Genetics,2000,155(2):574-578
[28]Evanno G,Regnaut S,Goudet J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular Ecology,2005,14(8):2611-2620
[29]Lu Y,Waller D.Genetic variability is correlated with population size and reproduction in American wild rice(Zizania palustris var.palustris,Poaceae)populations[J].American Journal of Botany,2005,92(6):990-997
[30]Hamrick J L,Godt M J W.Effects of life history traits on genetic diversity in plant species[J].Philosophical Transactions of the Royal Society Biological Sciences,1996,351(1345):1291-1298
[31]Guadagnuolo R,Bianchi D S,Felber F.Specific genetic markers for wheat,spelt,and four wild relatives:comparison of isozymes,RAPDs,and wheat microsatellites[J].Genome,2001,44(4):610-621
[32]Oelke E A.Wild Rice:Domestication of a Native North American Genus,New Crops[M].New York:Wiley,1993:235-243
[33]Kennard W,Phillips R,Porter R,Grombacher A,Phillips R L.A comparative map of wild rice(Zizania palustris L.2n=2x=30)[J].Theoretical and Applied Genetics,1999,99(5):793-799
[34]Guo L B,Qiu J,Han Z J,Ye Z H,Chen C,Liu C J,Xin X F,Ye C Y,Wang Y Y,Xie H Q,Wang Y,Bao J D,Tang S,Xu J,Gui Y J,Fu F,Wang W D,Zhang X C,Zhu Q H,Guang X M,Wang C Z,Cui H F,Cai D G,Ge S,Tuskan G A,Yang X H,Qian Q,He S Y,Wang J,Zhou X P,Fan L J.A host plant genome(Zizania latifolia)after a century‐long endophyte infection[J].Plant Journal,2015,83(4):600-609
[35]Shan X H,Ou X F,Liu Z L,Dong Y Z,Lin X Y,Li X W,Liu B.Transpositional activation of m Ping in an asymmetric nuclear somatic cell hybrid of rice and Zizania latifolia was accompanied by massive element loss[J].Theoretical and Applied Genetics,2009,119(7):1325-1333
[36]Wang N N,Wang H Y,Hui W,Zhang D,Wu Y,Ou X F,Liu S,Dong Z Y,Liu B.Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia[J].BMC Plant Biology,2010,10(1):190
[37]Dong Z Y,Wang H Y,Dong Y Z,Wang Y M,Liu W,Miao G J,Lin X Y,Wang D Q,Liu B.Extensive microsatellite variation in rice induced by introgression from wild rice(Zizania latifolia Griseb.)[J].PLo S One,2013,8(4):e62317-e62317
[2]翟成凯,孙桂菊.中国菰资源及其应用价值的研究[J].资源科学,2000,22(6):22-26
[3]Guo H B,Li S M,Peng J,Ke W D.Zizania latifolia Turcz.cultivated in China[J].Genetic Resources and Crop Evolution,2007,54(6):1211-1217
[4]官少飞,郎青,张本.鄱阳湖水生植被[J].水生生物学报,1987,11(1):9-21
[5]彭晓赟,赵运林,雷存喜,戴枚斌,易合成.菰对南洞庭湖湿地土壤中Cu、Sb、Cd、Pb的吸收与富集[J].中国农学通报,2009,(13):206-210
[6]Yu X,K9nig T,Zhang Q,Gao Y S.Nitrogen and phosphorus removal of locally adapted plant species used in constructed wetlands in China[J].Water Science and Technology,2012,66(4):695-703
[7]付硕章,柯文山,陈世俭.洪湖湿地野菰群落储碳、固碳功能研究[J].湖北大学学报(自然科学版),2013,35(3):393-396
[8]闫宁,王晓清,王志丹,张艳丽,薛惠民,郭得平.食用黑粉菌侵染对茭白植株抗氧化系统和叶绿素荧光的影响[J].生态学报,2013,33(5):1584-1593
[9]Przybylski R,Klensporfpawlik D,Anwar F,Rudzinska M.Lipid components of North American wild rice(Zizania palustris)[J].Journal of the American Oil Chemists'Society,2009,86(6):553-559
[10]Han S F,Zhang H,Zhai C K.Protective potentials of wild rice(Zizania latifolia(Griseb)Turcz)against obesity and lipotoxicity induced by a high-fat/cholesterol diet in rats[J].Food and Chemical Toxicology,2012,50(7):2263-2269
[11]Han S F,Zhang H,Qin L Q,Zhai C K.Effects of dietary carbohydrate replaced with wild rice(Zizania latifolia(Griseb)Turcz)on insulin resistance in rats fed with a high-fat/cholesterol diet[J].Nutrients,2013,5(2):552-564
[12]Lee S S,Baek Y S,Eun C S,Yu M H,Baek N I,Chung D K,Bang M H,Yang S A.Tricin derivatives as anti-inflammatory and anti-allergic constituents from the aerial part of Zizania latifolia[J].Bioscience Biotechnology and Biochemistry,2015,79(5):700-706
[13]吴国林.基于ISSR和SSR标记的鄱阳湖流域野生菰种质资源遗传多样性研究[D].南昌:江西农业大学,2014:29-30
[14]任绪瑞,刘艳玲,杨美,陈媛媛,王冬良,陈友根.菰SRAPPCR反应体系的优化与建立[J].热带作物学报,2014,35(2):299-306
[15]郑道君,云天海,张治礼,邓长智,谢良商.中国南瓜海南农家品种资源遗传多样性和亲缘关系研究[J].核农学报,2016,30(5):869-877
[16]戴智慧,龙骏,俞雷民,倪穗.宁波地区野生换锦花遗传多样性的ISSR分析[J].核农学报,2016,30(10):1898-1905
[17]Xu X W,Ke W D,Yu X P,Wen J,Ge S.A preliminary study on population genetic structure and phylogeography of the wild and cultivated Zizania latifolia(Poaceae)based on Adh1a sequences[J].Theoretical and Applied Genetics,2008,116(6):35-43
[18]Chen Y Y,Chu H J,Liu H,Liu Y L.Abundant genetic diversity of the wild rice Zizania latifolia in central China revealed by microsatellites[J].Annals of Applied Biology,2012,161(2):192-201
[19]Xu X W,Wu J W,Qi M X,Lu Q X,Lee P F,Lutz S,Ge S,Wen J.Comparative phylogeography of the wild-rice genus Zizania(Poaceae)in eastern Asia and North America[J].American Journal of Botany,2015,102(2):239-247
[20]王惠梅,吴国林,江绍琳,黄奇娜,奉保华,黄长干,江绍玫,吴建利.基于SSR和ISSR鄱阳湖流域野生菰(Zizania latifolia)资源的遗传多样性分析[J].植物遗传资源学报,2015,16(1):133-141
[21]苗培明,范玲,师维军,乌买尔江,徐利民,马君.棉花种质资源遗传多样性的TRAP分析[J].棉花学报,2009,21(5):420-426
[22]奉保华.水稻淡褐斑叶突变体lbsl1的鉴定、遗传分析与基因定位[D].北京:中国农业科学院,2012:27-28
[23]Richards C M,Reilley A,Touchell D,Antolin M F,Walterset C.Microsatellite primers for Texas wild rice(Zizania texana),and a preliminary test of the impact of cryogenic storage on allele frequency at these loci[J].Conservation Genetics,2004,5(6):853-859
[24]Quan Z,Pan L,K W D,Liu Y,Ding Y.Sixteen polymorphic microsatellite markers from Zizania latifolia Turcz.(Poaceae)[J].Molecular Ecology Resources,2009,9(3):887-889
[25]李晓红,张慧,王德元,张莉,邵剑文,张小平.我国特有植物青檀遗传结构的ISSR分析[J].生态学报,2013,33(16):4892-4901
[26]Yeh F C,Yang R,Boyle T.POPGENE Version 1.32.Microsoft windows-based freeware for population genetic analysis[EB/OL].(2016-10-10)[2017-03-01].https://sites.ualberta.ca/~fyeh/popgene.html
[27]Pritchard J K,Stephens M,Donnelly P.Inference of population structure using multilocus genotype data[J].Genetics,2000,155(2):574-578
[28]Evanno G,Regnaut S,Goudet J.Detecting the number of clusters of individuals using the software STRUCTURE:a simulation study[J].Molecular Ecology,2005,14(8):2611-2620
[29]Lu Y,Waller D.Genetic variability is correlated with population size and reproduction in American wild rice(Zizania palustris var.palustris,Poaceae)populations[J].American Journal of Botany,2005,92(6):990-997
[30]Hamrick J L,Godt M J W.Effects of life history traits on genetic diversity in plant species[J].Philosophical Transactions of the Royal Society Biological Sciences,1996,351(1345):1291-1298
[31]Guadagnuolo R,Bianchi D S,Felber F.Specific genetic markers for wheat,spelt,and four wild relatives:comparison of isozymes,RAPDs,and wheat microsatellites[J].Genome,2001,44(4):610-621
[32]Oelke E A.Wild Rice:Domestication of a Native North American Genus,New Crops[M].New York:Wiley,1993:235-243
[33]Kennard W,Phillips R,Porter R,Grombacher A,Phillips R L.A comparative map of wild rice(Zizania palustris L.2n=2x=30)[J].Theoretical and Applied Genetics,1999,99(5):793-799
[34]Guo L B,Qiu J,Han Z J,Ye Z H,Chen C,Liu C J,Xin X F,Ye C Y,Wang Y Y,Xie H Q,Wang Y,Bao J D,Tang S,Xu J,Gui Y J,Fu F,Wang W D,Zhang X C,Zhu Q H,Guang X M,Wang C Z,Cui H F,Cai D G,Ge S,Tuskan G A,Yang X H,Qian Q,He S Y,Wang J,Zhou X P,Fan L J.A host plant genome(Zizania latifolia)after a century‐long endophyte infection[J].Plant Journal,2015,83(4):600-609
[35]Shan X H,Ou X F,Liu Z L,Dong Y Z,Lin X Y,Li X W,Liu B.Transpositional activation of m Ping in an asymmetric nuclear somatic cell hybrid of rice and Zizania latifolia was accompanied by massive element loss[J].Theoretical and Applied Genetics,2009,119(7):1325-1333
[36]Wang N N,Wang H Y,Hui W,Zhang D,Wu Y,Ou X F,Liu S,Dong Z Y,Liu B.Transpositional reactivation of the Dart transposon family in rice lines derived from introgressive hybridization with Zizania latifolia[J].BMC Plant Biology,2010,10(1):190
[37]Dong Z Y,Wang H Y,Dong Y Z,Wang Y M,Liu W,Miao G J,Lin X Y,Wang D Q,Liu B.Extensive microsatellite variation in rice induced by introgression from wild rice(Zizania latifolia Griseb.)[J].PLo S One,2013,8(4):e62317-e62317