单克隆抗体技术在抗生素类兽药残留检测中的应用研究进展
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摘要
单克隆抗体技术是一种免疫学技术,具有特异性、多样性和定向性等特点,是现代生命科学研究和诊断的重要工具。文章介绍了单克隆抗体制备技术及其近年来在快速检测动物源性食品中抗生素类兽药残留的应用研究进展,以期为抗生素类兽药免疫学检测方法的应用与开发提供参考。
Monoclonal antibody technology is a kind of immunological techniques,which has the characteristics of specificity,diversity and orientation,and is an important tool in modern life science research and diagnosis. In this paper,the production of monoclonal antibody and its application in rapid detection of veterinary antibiotics residues in animal derived food in recent years are summarized,which may provide a reference for application and development of detection of veterinary antibiotics.
Monoclonal antibody technology is a kind of immunological techniques,which has the characteristics of specificity,diversity and orientation,and is an important tool in modern life science research and diagnosis. In this paper,the production of monoclonal antibody and its application in rapid detection of veterinary antibiotics residues in animal derived food in recent years are summarized,which may provide a reference for application and development of detection of veterinary antibiotics.
引文
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[21]唐慧林,赵瑜,林松,等.原奶中氨基糖苷类抗生素残留快速检测试纸条的研制[J].中国卫生检验杂志,2012,22(4):684-687.
[22]XU Naifeng,QU Changlong,MA Wei,et al.Development and application of one-step ELISA for the detection of neomycin in milk[J].Food Agr Immunol,2011,22(22):259-269.
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[24]韩青,王静,徐豪,等.牛奶中替米考星间接竞争酶联免疫吸附检测法的建立[J].中国畜牧兽医,2015,42(10):2625-2630.
[25]聂雯莹,唐承明,王建霞,等.牛奶中β-内酰胺类和四环素类药物胶体金快速检测方法的建立[J].中国畜牧兽医,2013,40(12):70-75.
[26]CHEN Yanni,KONG Dezhao,LIU Liqiang,et al.Development of an ELISA and immunochromatographic assay for tetracycline,oxytetracycline,and chlortetracycline residues in milk and honey based on the class-specific monoclonal antibody[J].Food Anal Method,2016,9(4):2-10.
[27]张立辰,生威,胡高爽,等.基于氯霉素单克隆抗体的ELISA方法的建立[J].食品研究与开发,2015,36(24):127-130.
[28]薛金英.酶联免疫吸附法(ELISA)检测鸡肉中氯霉素[J].中国动物保健,2016,18(10):79-80.
[29]LIU Guoyan,CHAI Chunyan.Towards the development of a sensitive electrochemical sensor for the determination of chloramphenicol residues in milk[J].Anal Method,2015,7(4):1572-1576.
[30]KARASECA N A,ERMOLAEVA T N.Piezoelectric immunosensors for the detection of individual antibiotics and the total content of penicillin antibiotics in foodstuffs[J].Talanta,2014,120(120):312-317.
[2]IBRAHIM H M,ABDELGHAFFAR F,OSMAN G Y,et al.Prevalence of Toxoplasma gondii in chicken samples from delta of Egypt using ELISA,histopathology and immunohistochemistry[J].J Parasit Dis,2016,40(2):485-489.
[3]NITIN G,RAMA C,KUMAR T C.Determination of cutoff of ELISA and immunofluorescence assay for scrub typhus[J].J Glob Infect Dis,2016,8(3):97-101.
[4]TITTLEMIER S A,GELINAS J M,DUFRESNE G,et al.Development of a direct competitive enzyme-linked immunosorbent assay for the detection of fluoroquinolone residues in shrimp[J].Food Anal Method,2008,1(1):28-35.
[5]MI Tiejun,WANG Zhanhui,EREMIN S A,et al.Simultaneous determination of multiple(fluoro)quinolone antibiotics in food samples by a one-step fluorescence polarization immunoassay[J].J Agric Food Chem,2013,61(39):9347-9355.
[6]ZENG Haopeng,CHEN Jiahong,ZHANG Chijian,et al.Broadspecificity chemiluminescence enzyme immunoassay for(fluoro)quinolones:hapten design and molecular modeling study of antibody recognition[J].Anal Chem,2016,88(7):3909-3916.
[7]CHEN Yiqiang,CHEN Qian,Han Miaomiao,et al.Near-infrared fluorescence-based multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk[J].Biosens Bioelectron,2016,79(4):430-434.
[8]马玲,韦建兴,陆忠础,等.磺胺二甲嘧啶间接竞争化学发光酶免疫分析法的建立及应用[J].中国食品卫生杂志,2014,26(5):455-460.
[9]张佳宜,张燕,王硕.胶体金免疫层析法快速检测猪肉及牛奶中磺胺二甲嘧啶残留[J].中国食品学报,2010,10(1):181-185.
[10]韩静,刘恩梅,王帅,等.胶体金免疫层析法检测食品中的磺胺类药物残留[J].现代食品科技,2011,27(5):603-606.
[11]TANG Yong,XU Jinting,WANG Wuzhou,et al.A sensitive immunochromatographic assay using colloidal gold-antibody probe for the rapid detection of semicarbazide in meat speciments[J].Eur Food Res Technol,2011,231(1):9-16.
[12]LI Shuqun,SONG Juan,YANG Hong,et al.An immunochromatographic assay for rapid and direct detection of 3-amino-5-morpholino-2-oxazolidone(AMOZ)in meat and feed samples[J].J Sci Food Agric,2014,94(4):760-767.
[13]LE Tao,XIE Yong,ZHU Liqian,et al.Rapid and sensitive detection of 3-amino-2-oxazolidinone using a quantum dot-based immunochromatographic fluorescent biosensor[J].J Agric Food Chem,2016,64(45):8678-8683.
[14]JESTER E,ABRAHAM A,WANG Yuesong,et al.Performance evaluation of commercial ELISA kits for screening of furazolidone and furaltadone residues in fish[J].Food Chem,2014,145(7):593-598.
[15]DENG Lihua,DAI Jinbo,XU Zhenlin,et al.Application of timeresolved fluroimmunoassay for determination of furaltadone metabolite 3-Amino-5-morpholinomethyl-2-oxazolidinone[J].Chin J Anal Chem,2016,44(8):1286-1290.
[16]WANG Wenbing,LIU Liqiang,XU Liguang,et al.Detection ofβ-lactamase residues in milk by sandwich ELISA[J].Inter J Env Res Pub Heal,2013,10(7):2688-2698.
[17]BROTO M,MATAS S,BABINGTON R,et al.Immunochemical detection of penicillins by using biohybrid magnetic particles[J].Food Control,2015,(51[b1]):381-389.
[18]孙志文,陶光灿,罗晓琴,等.胶体金免疫层析法测定牛奶中β-内酰胺类抗生素和三聚氰胺残留[J].乳业科学与技术,2012,35(3):39-42.
[19]XU Fei,JIANG Wenxiao,Zhou Jie,et al.Production of monoclonal antibody and development of a new immunoassay for apramycin in food[J].J Agric Food Chem,2014,62(14):3108-3113.
[20]王文珺,高隆轩,米振杰,等.大观霉素间接竞争ELISA的建立[J].动物医学进展,2016,37(1):43-46.
[21]唐慧林,赵瑜,林松,等.原奶中氨基糖苷类抗生素残留快速检测试纸条的研制[J].中国卫生检验杂志,2012,22(4):684-687.
[22]XU Naifeng,QU Changlong,MA Wei,et al.Development and application of one-step ELISA for the detection of neomycin in milk[J].Food Agr Immunol,2011,22(22):259-269.
[23]李向梅.牛奶中四种大环内酯类药物残留免疫检测技术研究[D].北京:中国农业大学,2016.
[24]韩青,王静,徐豪,等.牛奶中替米考星间接竞争酶联免疫吸附检测法的建立[J].中国畜牧兽医,2015,42(10):2625-2630.
[25]聂雯莹,唐承明,王建霞,等.牛奶中β-内酰胺类和四环素类药物胶体金快速检测方法的建立[J].中国畜牧兽医,2013,40(12):70-75.
[26]CHEN Yanni,KONG Dezhao,LIU Liqiang,et al.Development of an ELISA and immunochromatographic assay for tetracycline,oxytetracycline,and chlortetracycline residues in milk and honey based on the class-specific monoclonal antibody[J].Food Anal Method,2016,9(4):2-10.
[27]张立辰,生威,胡高爽,等.基于氯霉素单克隆抗体的ELISA方法的建立[J].食品研究与开发,2015,36(24):127-130.
[28]薛金英.酶联免疫吸附法(ELISA)检测鸡肉中氯霉素[J].中国动物保健,2016,18(10):79-80.
[29]LIU Guoyan,CHAI Chunyan.Towards the development of a sensitive electrochemical sensor for the determination of chloramphenicol residues in milk[J].Anal Method,2015,7(4):1572-1576.
[30]KARASECA N A,ERMOLAEVA T N.Piezoelectric immunosensors for the detection of individual antibiotics and the total content of penicillin antibiotics in foodstuffs[J].Talanta,2014,120(120):312-317.