HPLC法测定吡咯喹啉醌(PQQ)中咪唑并吡咯喹啉(IPQ)的含量
|
摘要
目的:建立吡咯喹啉醌(PQQ)中IPQ的含量测定方法。方法:采用TCI Kaseisorb LC ODS 2000色谱柱(4.6mm×150mm,5μm);流动相为乙腈-缓冲溶液(配制10mmol的磷酸氢二钾和15mmol的四丁基溴化铵水溶液,用磷酸调节p H至7.4)[28∶72];紫外检测器,检测波长为259nm;流速为1.0m L/min;进样量为20μL。结果:IPQ在0.05μg/m L～0.2μg/m L范围内线性关系良好,相关系数大于0.999。IPQ的回收率在104%～113%之间,RSD最大为3%。结论:该方法具有良好的专属性、检测灵敏度、精密度、线性和准确度,适用于PQQ中IPQ的质量控制。
Objective :To establish a method for determination of IPQ in pyrroloquinoline quinone(PQQ). Methods :TCI Kaseisorb LC ODS 2000 analytical column(4.6 mm×150 mm,5μm)was adopted with the mobile phase of acetonitrile-Buffer solution(prepare a solution containing 10 m M dibasic potassium phosphate and 15 m M tetrabutylammonium bromide in water,adjust with phosphoric acid to a p H of 7.4)[28 ∶ 72]at a flow rate of 1.0 m L/min. The detection wavelength was at 259 nm and the injection volume was 20μL. Results:The method had good linear relationship within the range 0.05μg/m L ~ 0.2μg/m L for IPQ,with the correlation coefficients were greater than 0.999. IPQ recoveries were between 104% and 113%,with maximum RSD of 3%. Conclusion:The method has good specificity,sensitivity,precision,linearity,accuracy and can be used for quality control of IPQ in PQQ.
Objective :To establish a method for determination of IPQ in pyrroloquinoline quinone(PQQ). Methods :TCI Kaseisorb LC ODS 2000 analytical column(4.6 mm×150 mm,5μm)was adopted with the mobile phase of acetonitrile-Buffer solution(prepare a solution containing 10 m M dibasic potassium phosphate and 15 m M tetrabutylammonium bromide in water,adjust with phosphoric acid to a p H of 7.4)[28 ∶ 72]at a flow rate of 1.0 m L/min. The detection wavelength was at 259 nm and the injection volume was 20μL. Results:The method had good linear relationship within the range 0.05μg/m L ~ 0.2μg/m L for IPQ,with the correlation coefficients were greater than 0.999. IPQ recoveries were between 104% and 113%,with maximum RSD of 3%. Conclusion:The method has good specificity,sensitivity,precision,linearity,accuracy and can be used for quality control of IPQ in PQQ.
引文
[1]张鹏,孙慧玲,孙静娴,等.吡咯喹啉醌(PQQ)营养作用研究进展[J].现代生物医学进展,2014,14(10):1987-1990.
[2]夏雨,周景文,陈坚.吡咯喹啉醌高通量检测方法的建立与优化[J].食品与生物技术学报,2017,36(2):122-128.
[3]魏静远,李大攀,赵炜楠,等.高效液相色谱法分析吡咯喹啉醌[J].生物技术通讯,2015,26(2):241-244.
[4]周杏琴,钦晓峰,张建康,等.离子对色谱法研究吡咯并喹呤醌与D-丝氨酸反应的化学动力学[J].色谱,2012,30(2):196-200.
[5]杨延新,熊向华,游松,等. 3种检测吡咯喹啉醌的方法比较[J].生物技术通讯,2011,22(4):544-547.
[2]夏雨,周景文,陈坚.吡咯喹啉醌高通量检测方法的建立与优化[J].食品与生物技术学报,2017,36(2):122-128.
[3]魏静远,李大攀,赵炜楠,等.高效液相色谱法分析吡咯喹啉醌[J].生物技术通讯,2015,26(2):241-244.
[4]周杏琴,钦晓峰,张建康,等.离子对色谱法研究吡咯并喹呤醌与D-丝氨酸反应的化学动力学[J].色谱,2012,30(2):196-200.
[5]杨延新,熊向华,游松,等. 3种检测吡咯喹啉醌的方法比较[J].生物技术通讯,2011,22(4):544-547.