小分子阿胶抗疲劳、抗氧化及止血作用研究
|
摘要
目的探讨小分子阿胶的抗疲劳、抗氧化及止血作用。方法建立大鼠复合血虚模型,检测游泳力竭时间、血液学指标及血清超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(maleic dialdehyde,MDA)、脂质过氧化物(lipid peroxide,LPO)水平,考察小分子阿胶的抗疲劳、抗氧化作用。测定ICR小鼠出血时间(bleeding time,BT)、凝血时间(clotting time,CT)。建立大鼠血热出血模型和肝素化出血模型,考察小分子阿胶在止血方面的作用及可能的机制。结果与模型组比较,小分子阿胶各剂量均明显延长大鼠游泳力竭时间(P<0.05,P<0.01),明显降低血清MDA、LPO含量(P<0.05,P<0.01);小分子阿胶1.500、0.375 g·kg~(-1)明显升高大鼠血中淋巴细胞数量(P<0.05);3.00、1.50 g·kg~(-1)明显缩短小鼠BT和CT(P<0.05);1.500 g·kg~(-1)逆转延长的凝血酶原时间(P<0.05,P<0.01),逆转血细胞的不良改变。结论小分子阿胶具有抗疲劳、抗氧化、增强耐力的作用,并有止血的功效。
Aim To study the anti-fatigue,anti-oxidative and hemostatic effects of small molecule Asini Corii Colla(SMACC). Methods Rat model of complex blood deficiency was established to detect the exhausted time of swimming,hematology,superoxide dismu-tase(SOD),serum maleic dialdehyde(MDA), and blood deficiency was established to detect the exhausted time of swimming,hematology,superoxide dismutase( SOD),serum maleic dialdehyde( MDA),and lipid peroxide( LPO) levels,aiming to explore the anti-fatigue and anti-oxidative effects of SMACC. ICR mice were used to measure the blood clotting time( CT) and bleeding time( BT). Fevered and bleeding model and the heparinized bleeding model in rats were established to investigate the effect of SMACC in hemostasis and its possible mechanism. Results Compared with model group,SMACC significantly prolonged the swimming time of model rats(P<0.05,P<0.01)and decreased the content of serum MDA,LPO( P<0. 05,P<0.01) at doses of 1. 500,0. 750,0. 375 g·kg~(-1),and increased the number of lymphocytes in the blood at doses of 1. 500,0. 375 g·kg~(-1)( P<0.05).SMACC of 3. 00,1. 50 g · kg~(-1) significantly reduced the BT and CT( P<0.05). SMACC markedly reversed the prolonged prothrombin time( P<0.05) and the adverse changes of the hematological indicators.Conclusions SMACC has the pharmacological effects of anti-fatigue,anti-oxidation and enhancing endurance,and also the effects of hemostasis and convergence.
Aim To study the anti-fatigue,anti-oxidative and hemostatic effects of small molecule Asini Corii Colla(SMACC). Methods Rat model of complex blood deficiency was established to detect the exhausted time of swimming,hematology,superoxide dismu-tase(SOD),serum maleic dialdehyde(MDA), and blood deficiency was established to detect the exhausted time of swimming,hematology,superoxide dismutase( SOD),serum maleic dialdehyde( MDA),and lipid peroxide( LPO) levels,aiming to explore the anti-fatigue and anti-oxidative effects of SMACC. ICR mice were used to measure the blood clotting time( CT) and bleeding time( BT). Fevered and bleeding model and the heparinized bleeding model in rats were established to investigate the effect of SMACC in hemostasis and its possible mechanism. Results Compared with model group,SMACC significantly prolonged the swimming time of model rats(P<0.05,P<0.01)and decreased the content of serum MDA,LPO( P<0. 05,P<0.01) at doses of 1. 500,0. 750,0. 375 g·kg~(-1),and increased the number of lymphocytes in the blood at doses of 1. 500,0. 375 g·kg~(-1)( P<0.05).SMACC of 3. 00,1. 50 g · kg~(-1) significantly reduced the BT and CT( P<0.05). SMACC markedly reversed the prolonged prothrombin time( P<0.05) and the adverse changes of the hematological indicators.Conclusions SMACC has the pharmacological effects of anti-fatigue,anti-oxidation and enhancing endurance,and also the effects of hemostasis and convergence.
引文
[1] 国家药典委员会. 中华人民共和国药典: 一部[S]. 北京: 中国医药科技出版社,2015:189. Chinese Pharmacopoeia Commission. Pharmacopoeia of the People’s Republic of China: Part Ⅰ[S]. Beijing: China Medical Science and Technology Press,2015:189.
[2] 庞萌萌,李敏,田晨颖,等. 阿胶酶解液相对分子质量分布及其补血升白作用[J]. 中国实验方剂学杂志,2017,23(12):13-7. Pang M M,Li M,Tian C Y,et al. Distribution of relative molecular mass of Asini Corii Colla hydrolyzate and investigation of its blood enrichment and leukogenic effect [J]. Chin J Exp Tradit Med Formul,2017,23(12):13-7.
[3] Moure A,Dominguez H,Parajo J C. Antioxidant properties of ultrafiltration recovered soy protein fractions from industrial effluents and their hydrolysates [J]. Process Biochem,2006,41(2):447-56.
[4] 刘元涛,张惠惠,王升光,等. 阿胶仿生酶解前后提高免疫力作用对比研究[J]. 时珍国医国药,2016,27(9):2158-60. Liu Y T,Zhang H H,Wang S G,et al. Comparative study on enhancing immune function before and after enzymatic hydrolysis of Asini Corii Colla [J]. Lishizhen Med Mater Med Res,2016,27(9):2158-60.
[5] 李敏,庞萌萌,田晨颖,等. 不同阿胶酶解液相对分子量分布及补血升白作用对比研究[J]. 中国食品添加剂,2017(6):105-11. Li M,Pang M M,Tian C Y,et al. Molecular weight distribution in different donkey-hide gelatin enzymolysis solution and its pharmacology effects[J]. China Food Addit,2017(6):105-11.
[6] 焦立红,任雷鸣,赵寿康. 四种血虚小鼠模型制备方法的比较[J]. 时珍国医国药,2006,17(6):1001-2. Jiao L H,Ren L M,Zhao S K. Comparison of the preparation methods of four blood deficiency mice model [J]. Lishizhen Med Mater Med Res,2006,17(6):1001-2.
[7] 包贝华,赵显,曹雨诞,等. 鸡冠花对致热复合出血模型大鼠的凉血止血效应机制研究[J]. 中国药理学通报,2013,29(10):1457-61. Bao B H,Zhao X,Cao Y D,et al. Cooling blood and haemostatic mechanism of Celosia Cristatae Flos in rat model of blood-heat and hemorrhage syndrome [J]. Chin Pharmacol Bull,2013,29(10):1457-61.
[8] 刘正君,吉延慧,张琪嘉钰,等. 三七止血作用的实验研究[J]. 陕西中医学院学报,2015,38(2):71-7. Liu Z J,Ji Y H,Zhangqi J Y,et al. The experimental researches on hemostasis function of Sanqi [J]. J Shanxi Coll Tradit Chin Med,2015,38(2):71-7.
[9] 李伟霞,唐于平,郭建明,等. 当归-川芎药对及其组成药味对3种血虚模型补血作用的比较研究[J]. 中国中药杂志,2011,36(13):1808-14. Li W X,Tang Y P,Guo J M,et al. Enriching blood effect comparison in three kinds of blood deficiency model after oral administration of drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb [J]. China J Chin Mater Med,2011,36(13):1808-14.
[10] 李淑娇,唐于平,李伟霞,等. 当归-红花不同配比对血虚小鼠补血作用的比较研究[J]. 中国药理学通报,2014,30(6):874-9. Li S J,Tang Y P,Li W X,et al. Comparing tonifying blood effects of Danggui-Honghua with different proportions on blood deficiency mice [J]. Chin Pharmacol Bull,2014,30(6):874-9.
[11] 金若敏,宁炼,陈长勋,等. 血虚模型动物制备及当归补血汤的作用研究[J]. 中成药,2001,23(4):268-71. Jin R M,Ning L,Chen C X,et al. Preparation of blood-deficient model and effects of Danggui blood-supplementing decoction on hemopoiesis [J]. Chin Tradit Pat Med,2001,23(4):268-71.
[12] 李吉萍,孙婷婷,胡天骄,刘墨祥. 板蓝根活性组分对小鼠耐缺氧及抗疲劳作用的实验研究[J]. 中国药理学通报,2016,32(5):712-5. Li J P,Sun T T,Hu T J,Liu M X. Effects of active component of Radix Isatidis on anti-anoxia and anti-fatigue in mice[J]. Chin Pharmacol Bull,2016,32(5):712-5.
[13] 赵菲菲,杨馨,徐丹,等. 白及非多糖组分的止血作用及其机制的初步研究[J]. 中国药理学通报,2016,32(8):1121-6. Zhao F F,Yang X,Xu D,et al. Hemostatic effect and mechanism of a non-polysaccharide fraction of Bletilla striata [J]. Chin Pharmacol Bull,2016,32(8):1121-6.
[14] 于生,陈星,单鸣秋. 紫菀丸对血热出血大鼠血液流变性、凝血时间及肺部的影响[J]. 中国实验方剂学杂志,2013,19(13):229-33. Yu S,Chen X,Shan M Q. Effects of Ziwan Wan on hemorheological parameters,clotting time and lung presentations in rats with fevered and bleeding [J]. Chin J Exp Tradit Med Formul,2013,19(13): 229-33.
[2] 庞萌萌,李敏,田晨颖,等. 阿胶酶解液相对分子质量分布及其补血升白作用[J]. 中国实验方剂学杂志,2017,23(12):13-7. Pang M M,Li M,Tian C Y,et al. Distribution of relative molecular mass of Asini Corii Colla hydrolyzate and investigation of its blood enrichment and leukogenic effect [J]. Chin J Exp Tradit Med Formul,2017,23(12):13-7.
[3] Moure A,Dominguez H,Parajo J C. Antioxidant properties of ultrafiltration recovered soy protein fractions from industrial effluents and their hydrolysates [J]. Process Biochem,2006,41(2):447-56.
[4] 刘元涛,张惠惠,王升光,等. 阿胶仿生酶解前后提高免疫力作用对比研究[J]. 时珍国医国药,2016,27(9):2158-60. Liu Y T,Zhang H H,Wang S G,et al. Comparative study on enhancing immune function before and after enzymatic hydrolysis of Asini Corii Colla [J]. Lishizhen Med Mater Med Res,2016,27(9):2158-60.
[5] 李敏,庞萌萌,田晨颖,等. 不同阿胶酶解液相对分子量分布及补血升白作用对比研究[J]. 中国食品添加剂,2017(6):105-11. Li M,Pang M M,Tian C Y,et al. Molecular weight distribution in different donkey-hide gelatin enzymolysis solution and its pharmacology effects[J]. China Food Addit,2017(6):105-11.
[6] 焦立红,任雷鸣,赵寿康. 四种血虚小鼠模型制备方法的比较[J]. 时珍国医国药,2006,17(6):1001-2. Jiao L H,Ren L M,Zhao S K. Comparison of the preparation methods of four blood deficiency mice model [J]. Lishizhen Med Mater Med Res,2006,17(6):1001-2.
[7] 包贝华,赵显,曹雨诞,等. 鸡冠花对致热复合出血模型大鼠的凉血止血效应机制研究[J]. 中国药理学通报,2013,29(10):1457-61. Bao B H,Zhao X,Cao Y D,et al. Cooling blood and haemostatic mechanism of Celosia Cristatae Flos in rat model of blood-heat and hemorrhage syndrome [J]. Chin Pharmacol Bull,2013,29(10):1457-61.
[8] 刘正君,吉延慧,张琪嘉钰,等. 三七止血作用的实验研究[J]. 陕西中医学院学报,2015,38(2):71-7. Liu Z J,Ji Y H,Zhangqi J Y,et al. The experimental researches on hemostasis function of Sanqi [J]. J Shanxi Coll Tradit Chin Med,2015,38(2):71-7.
[9] 李伟霞,唐于平,郭建明,等. 当归-川芎药对及其组成药味对3种血虚模型补血作用的比较研究[J]. 中国中药杂志,2011,36(13):1808-14. Li W X,Tang Y P,Guo J M,et al. Enriching blood effect comparison in three kinds of blood deficiency model after oral administration of drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb [J]. China J Chin Mater Med,2011,36(13):1808-14.
[10] 李淑娇,唐于平,李伟霞,等. 当归-红花不同配比对血虚小鼠补血作用的比较研究[J]. 中国药理学通报,2014,30(6):874-9. Li S J,Tang Y P,Li W X,et al. Comparing tonifying blood effects of Danggui-Honghua with different proportions on blood deficiency mice [J]. Chin Pharmacol Bull,2014,30(6):874-9.
[11] 金若敏,宁炼,陈长勋,等. 血虚模型动物制备及当归补血汤的作用研究[J]. 中成药,2001,23(4):268-71. Jin R M,Ning L,Chen C X,et al. Preparation of blood-deficient model and effects of Danggui blood-supplementing decoction on hemopoiesis [J]. Chin Tradit Pat Med,2001,23(4):268-71.
[12] 李吉萍,孙婷婷,胡天骄,刘墨祥. 板蓝根活性组分对小鼠耐缺氧及抗疲劳作用的实验研究[J]. 中国药理学通报,2016,32(5):712-5. Li J P,Sun T T,Hu T J,Liu M X. Effects of active component of Radix Isatidis on anti-anoxia and anti-fatigue in mice[J]. Chin Pharmacol Bull,2016,32(5):712-5.
[13] 赵菲菲,杨馨,徐丹,等. 白及非多糖组分的止血作用及其机制的初步研究[J]. 中国药理学通报,2016,32(8):1121-6. Zhao F F,Yang X,Xu D,et al. Hemostatic effect and mechanism of a non-polysaccharide fraction of Bletilla striata [J]. Chin Pharmacol Bull,2016,32(8):1121-6.
[14] 于生,陈星,单鸣秋. 紫菀丸对血热出血大鼠血液流变性、凝血时间及肺部的影响[J]. 中国实验方剂学杂志,2013,19(13):229-33. Yu S,Chen X,Shan M Q. Effects of Ziwan Wan on hemorheological parameters,clotting time and lung presentations in rats with fevered and bleeding [J]. Chin J Exp Tradit Med Formul,2013,19(13): 229-33.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |