醋炙乌药-木香对肝郁气滞型功能性消化不良模型大鼠胃排空及胃肠激素的影响
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摘要
目的:探讨醋炙乌药-木香对肝郁气滞型功能性消化不良(FD)模型大鼠胃排空及胃肠激素的影响。方法:将60只SD大鼠随机分为空白组(n=10)和造模组(n=50),造模组大鼠采用慢性应激束缚或食物剥夺或过度疲劳等多种手段复制肝郁气滞型FD模型。造模成功后,将造模组大鼠随机分为模型组(生理盐水)、醋炙乌药组(以生药量计为1.62 g/kg)、木香组(以生药量计为1.62 g/kg)、醋炙乌药-木香组(1∶1,m/m,以生药量计为1.62 g/kg)和莫沙必利组(阳性对照,1.35 mg/kg),每组10只。各给药组大鼠每天灌胃相应药物1次,空白组和模型组大鼠灌胃等体积生理盐水,连续给药14 d。末次给药2 h后,采用酚红含量法检测各组大鼠的胃排空率和小肠推进率;苏木精-伊红(HE)染色后,显微镜下观察其胃窦部组织病理学变化;并采用酶联免疫吸附法检测其血清中胃动素(MTL)、胃泌素(GAS)、胆囊收缩素(CCK)含量。结果:与空白组比较,模型组大鼠胃排空率、小肠推进率显著降低(P<0.01);血清中MTL、GAS含量显著降低(P<0.01),CCK含量显著升高(P<0.01);各造模组大鼠造模后均未出现器质性损伤。与模型组、醋炙乌药组、木香组比较,醋炙乌药-木香组和莫沙必利组大鼠胃排空率和小肠推进率显著升高(P<0.05或P<0.01);血清中MTL、GAS含量显著升高(P<0.01),CCK含量显著降低(P<0.01)。结论:醋炙乌药-木香能加速肝郁气滞型FD模型大鼠胃排空及小肠推进,升高其血清中MTL、GAS含量和降低血清中CCK含量,且其作用优于两药单用。
OBJECTIVE:To investigate the effects of Lindera aggregate of stir-baking with vinegar-Aucklandia lappa on gastric emptying and gastrointestinal hormones in functional dyspepsia liver depression and qi stagnation(FDLDQS) model rats.METHODS:Totally 60 SD rats were randomly divided into blank group(n=10)and modeling group(n=50);FDLDQS model was induced by chronic stress restraint or food deprivation or excessive fatigue in modeling group. After modeling,model rats were randomly divided into model group(normal saline),L. aggregate of stir-baking with vinegar group(1.62 g/kg,calculated by crude drug),A. lappa group(1.62 g/kg,calculated by crude drug),L. aggregate of stir-baking with vinegar-A. lappa group(1 ∶1,m/m,1.62 g/kg,calculated by crude drug) and mosapride group(positive control,1.35 mg/kg),with 10 rats in each group.Administration groups were given relevant medicine intragastrically once a day;blank group and model group were given constant volume of normal saline intragastrically,for consecutive 14 d. 2 h after last medication,gastric emptying rate and small intestinal propulsion rate of rats in each group were measured by phenol red content method. After HE staining,the morphological changes of antrum tissue were observed under microscope. The contents of motilin(MTL),gastrin(GAS)and cholecystokinin(CCK)in serum were determined by ELISA method. RESULTS:Compared with blank group,gastric emptying rate and small intestinal propulsion rate of rats were decreased significantly in model group(P<0.01);the serum contents of MTL and GAS were decreased significantly(P<0.01),while the content of CCK was increased significantly(P<0.01). No organic damage was found in all model groups. Compared with model group,L. aggregate stir-baking with vinegar group and A. lappa group,gastric emptying rate and small intestinal propulsion rate of rats were increased significantly in L. aggregate of stir-baking with vinegar-A.lappa group and mosapride group(P<0.05 or P<0.01);serum contents of MTL and GAS were increased significantly(P<0.01),while the content of CCK was decreased significantly(P<0.01). CONCLUSIONS:L. aggregate of stir-baking with vinegar-A. lappa can accelerate gastric emptying and small intestinal propulsion rate of FDLDQS rats,increase serum contents of MTL and GAS but decrease the content of CCK;its effects are better than that of them alone.
OBJECTIVE:To investigate the effects of Lindera aggregate of stir-baking with vinegar-Aucklandia lappa on gastric emptying and gastrointestinal hormones in functional dyspepsia liver depression and qi stagnation(FDLDQS) model rats.METHODS:Totally 60 SD rats were randomly divided into blank group(n=10)and modeling group(n=50);FDLDQS model was induced by chronic stress restraint or food deprivation or excessive fatigue in modeling group. After modeling,model rats were randomly divided into model group(normal saline),L. aggregate of stir-baking with vinegar group(1.62 g/kg,calculated by crude drug),A. lappa group(1.62 g/kg,calculated by crude drug),L. aggregate of stir-baking with vinegar-A. lappa group(1 ∶1,m/m,1.62 g/kg,calculated by crude drug) and mosapride group(positive control,1.35 mg/kg),with 10 rats in each group.Administration groups were given relevant medicine intragastrically once a day;blank group and model group were given constant volume of normal saline intragastrically,for consecutive 14 d. 2 h after last medication,gastric emptying rate and small intestinal propulsion rate of rats in each group were measured by phenol red content method. After HE staining,the morphological changes of antrum tissue were observed under microscope. The contents of motilin(MTL),gastrin(GAS)and cholecystokinin(CCK)in serum were determined by ELISA method. RESULTS:Compared with blank group,gastric emptying rate and small intestinal propulsion rate of rats were decreased significantly in model group(P<0.01);the serum contents of MTL and GAS were decreased significantly(P<0.01),while the content of CCK was increased significantly(P<0.01). No organic damage was found in all model groups. Compared with model group,L. aggregate stir-baking with vinegar group and A. lappa group,gastric emptying rate and small intestinal propulsion rate of rats were increased significantly in L. aggregate of stir-baking with vinegar-A.lappa group and mosapride group(P<0.05 or P<0.01);serum contents of MTL and GAS were increased significantly(P<0.01),while the content of CCK was decreased significantly(P<0.01). CONCLUSIONS:L. aggregate of stir-baking with vinegar-A. lappa can accelerate gastric emptying and small intestinal propulsion rate of FDLDQS rats,increase serum contents of MTL and GAS but decrease the content of CCK;its effects are better than that of them alone.
引文
[1]FUJIWARA Y,ARAKAWA T.Overlap in patients with dyspepsia/functional dyspepsia[J].J Neurogastroenterol Motil,2014,20(4):447-457.
[2]YUP LJ,NAYOUNG K,HA KG,et al.Comparing the areas of interest in the field of functional gastrointestinal disorder and neuro gastroenterology and motility between the east and the west[J].J Neurogastroenterol Motil,2015,21(4):503-510.
[3]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:77.
[4]温中京,陈海委.乌药的本草学考证[J].江西中医药,2009,40(3):57-59.
[5]李珊,陈文明,欧阳荣.乌药及其炮制品不同提取物镇痛作用的研究[J].中国现代应用药学,2015,32(11):1306-1308.
[6]周新蓓,欧阳荣.乌药不同炮制品中乌药醚内酯的含量测定[J].中药材,2008,31(3):350-351.
[7]左美玲,欧阳荣,皮晓华.乌药不同炮制品中乌药内酯含量测定[J].中国医药指南,2010,8(5):43-44.
[8]张宇翔.加味五磨饮子治疗慢传输型便秘的疗效及对患者血清P物质、血管活性肠肽、一氧化氮、神经肽Y水平的影响[J].中国老年学杂志,2016,36(16):4008-4009.
[9]王豪,罗志强,李梦薇,等.四磨汤口服液在大鼠体内的吸收代谢过程[J].中成药,2018,40(3):718-722.
[10]朱洁,王叶,郭璇,等.新型造模法制备功能性消化不良肝郁脾虚证大鼠模型[J].湖南中医药大学学报,2018,38(4):372-375.
[11]肖政华,谭芊任,崔峻松,等.慢性应激对小鼠胃肠运动及血清GAS、MTL的影响[J].贵阳中医学院学报,2018,40(1):23-26,54.
[12]刘晶,李峰,唐旭东,等.功能性消化不良动物模型及其在中医研究中的应用概述[J].环球中医药,2013,6(12):955-958.
[13]安晓霞,梁尧,王振刚,等.中医药治疗功能性消化不良的临床研究进展[J].中医药学报,2018,46(4):123-127.
[14]TACK J,TALLEY NJ,CAMILLERI M,et al.Functional gastroduodenal disorders[J].Gastroenterology,2006,130(5):1466-1479.
[15]BISSCHOPS R,KARAMANOLIS G,ARTS J,et al.Relationship between symptoms and ingestion of a meal in functional dyspepsia[J].Gut,2008,57(11):1495-1503.
[16]王丽红.和胃汤对功能性消化不良患者胃动素和胃泌素的影响[J].中药药理与临床,2015,31(6):182-183.
[17]王彪猛,刘德科,陈嘉屿.白术治疗胃肠道疾病作用机制的研究进展[J].中国药房,2015,26(17):2436-2438.
[18]潘炟,闫丽辉.胃肠安丸对功能性消化不良肝郁脾虚大鼠胃动素的影响[J].中草药,2016,47(20):3673-3676.
[19]余朝辉,郑珊娇.白枳养胃汤对功能性消化不良大鼠血清和组织中MTL、GAS和VIP含量的影响[J].中药药理与临床,2015,31(4):199-201.
[20]赛男,蔡光先,万胜.四磨汤治疗功能性消化不良(肝脾气滞证)的临床疗效及对血清NO、Ach E、CCK、SP的影响[J].中国中医急症,2014,23(10):1791-1792,1833.
[2]YUP LJ,NAYOUNG K,HA KG,et al.Comparing the areas of interest in the field of functional gastrointestinal disorder and neuro gastroenterology and motility between the east and the west[J].J Neurogastroenterol Motil,2015,21(4):503-510.
[3]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:77.
[4]温中京,陈海委.乌药的本草学考证[J].江西中医药,2009,40(3):57-59.
[5]李珊,陈文明,欧阳荣.乌药及其炮制品不同提取物镇痛作用的研究[J].中国现代应用药学,2015,32(11):1306-1308.
[6]周新蓓,欧阳荣.乌药不同炮制品中乌药醚内酯的含量测定[J].中药材,2008,31(3):350-351.
[7]左美玲,欧阳荣,皮晓华.乌药不同炮制品中乌药内酯含量测定[J].中国医药指南,2010,8(5):43-44.
[8]张宇翔.加味五磨饮子治疗慢传输型便秘的疗效及对患者血清P物质、血管活性肠肽、一氧化氮、神经肽Y水平的影响[J].中国老年学杂志,2016,36(16):4008-4009.
[9]王豪,罗志强,李梦薇,等.四磨汤口服液在大鼠体内的吸收代谢过程[J].中成药,2018,40(3):718-722.
[10]朱洁,王叶,郭璇,等.新型造模法制备功能性消化不良肝郁脾虚证大鼠模型[J].湖南中医药大学学报,2018,38(4):372-375.
[11]肖政华,谭芊任,崔峻松,等.慢性应激对小鼠胃肠运动及血清GAS、MTL的影响[J].贵阳中医学院学报,2018,40(1):23-26,54.
[12]刘晶,李峰,唐旭东,等.功能性消化不良动物模型及其在中医研究中的应用概述[J].环球中医药,2013,6(12):955-958.
[13]安晓霞,梁尧,王振刚,等.中医药治疗功能性消化不良的临床研究进展[J].中医药学报,2018,46(4):123-127.
[14]TACK J,TALLEY NJ,CAMILLERI M,et al.Functional gastroduodenal disorders[J].Gastroenterology,2006,130(5):1466-1479.
[15]BISSCHOPS R,KARAMANOLIS G,ARTS J,et al.Relationship between symptoms and ingestion of a meal in functional dyspepsia[J].Gut,2008,57(11):1495-1503.
[16]王丽红.和胃汤对功能性消化不良患者胃动素和胃泌素的影响[J].中药药理与临床,2015,31(6):182-183.
[17]王彪猛,刘德科,陈嘉屿.白术治疗胃肠道疾病作用机制的研究进展[J].中国药房,2015,26(17):2436-2438.
[18]潘炟,闫丽辉.胃肠安丸对功能性消化不良肝郁脾虚大鼠胃动素的影响[J].中草药,2016,47(20):3673-3676.
[19]余朝辉,郑珊娇.白枳养胃汤对功能性消化不良大鼠血清和组织中MTL、GAS和VIP含量的影响[J].中药药理与临床,2015,31(4):199-201.
[20]赛男,蔡光先,万胜.四磨汤治疗功能性消化不良(肝脾气滞证)的临床疗效及对血清NO、Ach E、CCK、SP的影响[J].中国中医急症,2014,23(10):1791-1792,1833.