选择性培养基易碎菌落法检出5株氰化钾阳性沙门菌
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摘要
目的 WS易碎菌落法检出5株氰化钾阳性沙门菌。方法将健康体检肛拭子标本在SF增菌后接种WS平板分离,再用WS易碎菌落法挑选疑似沙门菌做菌落鉴定。结果 13 278件肠检标本,易碎菌落法检出102株沙门菌,其中发现5株氰化钾阳性沙门菌,阳性率达4.9%。结论氰化钾试验大多数沙门菌为阴性,1976年-1980年我国沙门菌菌型分布调查协作组对19 729株沙门菌所作的生化试验结果氰化钾阳性率为0.01%,本实验室沙门菌氰化钾阳性率达4.9%。沙门菌氰化钾阳性同检验的标准相违背,所以按照国家标准或行业标准的流程极易漏检,WS易碎菌落法方法简单特异性强敏感性高,氰化钾阳性沙门菌应该引起大家的注意不要轻易丢弃,防止沙门菌漏检。
Objective Five strains of potassium cyanogen positive Salmonella were detected by WS method. Methods Swab specimens from healthy people were inoculated with WS plates after SF enrichment,and then the suspected Salmonella was selected by WS fragile colony method for identification. Results Of the 278 intestine specimens,102 strains of Salmonella were detected by the fragile colony method,and 5 strains of potassium cyanide-positive Salmonella were found,with a positive rate of 4. 9%. Conclusion Potassium cyanide test was negative for most Salmonella. From 1976 to 1980,the Salmonella type distribution survey team of China conducted a biochemical test on 19 729 strains of Salmonella. The positive rate of potassium cyanide was 0. 01%. But the positive rate of potassium was 4. 9% in this study. Salmonella potassium cyanide positive is contrary to the test standard,so miss detection is easy to happen according to the national standards or industry standard process. The WS fragile colony method is simple,specific and strong,and the potassium cyanide-positive Salmonella should cause attention,so as to avoid the miss detection of Salmonella.
Objective Five strains of potassium cyanogen positive Salmonella were detected by WS method. Methods Swab specimens from healthy people were inoculated with WS plates after SF enrichment,and then the suspected Salmonella was selected by WS fragile colony method for identification. Results Of the 278 intestine specimens,102 strains of Salmonella were detected by the fragile colony method,and 5 strains of potassium cyanide-positive Salmonella were found,with a positive rate of 4. 9%. Conclusion Potassium cyanide test was negative for most Salmonella. From 1976 to 1980,the Salmonella type distribution survey team of China conducted a biochemical test on 19 729 strains of Salmonella. The positive rate of potassium cyanide was 0. 01%. But the positive rate of potassium was 4. 9% in this study. Salmonella potassium cyanide positive is contrary to the test standard,so miss detection is easy to happen according to the national standards or industry standard process. The WS fragile colony method is simple,specific and strong,and the potassium cyanide-positive Salmonella should cause attention,so as to avoid the miss detection of Salmonella.
引文
[1]何晓青.卫生防疫细菌检验[M].南昌:新华出版社,1989:301-479.
[2]张缪伟,周兆华,沈美枫,等.WS易碎菌落法与显色培养基CAS检测沙门菌结果对比[J].中国卫生检验杂志,2014,24(9):1257-1258.
[3]张缪伟,邵燕,吴建中,等.利用WS琼脂特殊菌落挑选沙门氏菌[J].中国卫生检验杂志,2000,10(2):236-236.
[4]朱超,许学斌.沙门菌属血清型诊断[M].上海:同济大学出版社,2009:16-17.
[5]胡兴娟,周向阳,王忠发,等.一株罕见发酵蔗糖的贝坎道夫沙门菌鉴定与探讨[J].中国卫生检验杂志,2012,22(6):1324-1326.
[6]张缪伟,周兆华,沈美枫,等.沙门菌检测WS易碎菌落法与常规法对比试验[J].中国卫生检验杂志,2014,24(7):954-955.
[7]张缪伟,吴国强,吴建中,等.一名食品从业人员粪便中检出两株不同血清型沙门菌的报告[J].职业与健康,2005,21(8):1171-1172.
[8]邱颖,王海霞,王宇卓,等.2016年河北省三河市餐服人员沙门氏菌检出情况分析[J].医学动物防制,2018,34(1):97-98.
[9]董梅,史文凤,黄少平,等.2014年北京市房山区沙门菌血清学鉴定及耐药性分析[J].中国卫生检验杂志,2016,26(6):811-812.
[10]修宁宁,郑碧英,辛青松.2013年东莞市沙门菌感染流行病学特征及耐药性分析[J].中华临床医师杂志,2015,13(9):2530-2534.
[11]林金丽,梁玉潮,梁卫桥,等.公共场所从业人员沙门氏菌携带情况分析[J].当代医学,2013,19(23):160-161.
[12]杨晓,姜云,庞作章.大型活动饮食服务人员肠道致病菌检查结果分析[J].职业与健康,2013,29(16):2020-2024.
[13]陈延秋,廖亦红,曹春远.2006-2008年龙岩市新罗区从业人员体检结果分析[J].职业与健康,2010,26(6):661-662.
[14]张新,王全意,曲梅,等.2009-2011年北京市腹泻儿童沙门菌感染流行病学特征和耐药分析[J].中国病原生物学杂志,2012,7(10):728-730.
[15]宿飞,沈福杰,王怡珺,等.上海市黄浦区2008-2011年沙门菌感染流行病学分析[J].上海预防医学,2013,25(1):4-6.
[16]付秀影,黄露.一起鼠伤寒沙门氏菌食物中毒的流行病学调查[J].首都公共卫生,2016,10(2):86-89.
[2]张缪伟,周兆华,沈美枫,等.WS易碎菌落法与显色培养基CAS检测沙门菌结果对比[J].中国卫生检验杂志,2014,24(9):1257-1258.
[3]张缪伟,邵燕,吴建中,等.利用WS琼脂特殊菌落挑选沙门氏菌[J].中国卫生检验杂志,2000,10(2):236-236.
[4]朱超,许学斌.沙门菌属血清型诊断[M].上海:同济大学出版社,2009:16-17.
[5]胡兴娟,周向阳,王忠发,等.一株罕见发酵蔗糖的贝坎道夫沙门菌鉴定与探讨[J].中国卫生检验杂志,2012,22(6):1324-1326.
[6]张缪伟,周兆华,沈美枫,等.沙门菌检测WS易碎菌落法与常规法对比试验[J].中国卫生检验杂志,2014,24(7):954-955.
[7]张缪伟,吴国强,吴建中,等.一名食品从业人员粪便中检出两株不同血清型沙门菌的报告[J].职业与健康,2005,21(8):1171-1172.
[8]邱颖,王海霞,王宇卓,等.2016年河北省三河市餐服人员沙门氏菌检出情况分析[J].医学动物防制,2018,34(1):97-98.
[9]董梅,史文凤,黄少平,等.2014年北京市房山区沙门菌血清学鉴定及耐药性分析[J].中国卫生检验杂志,2016,26(6):811-812.
[10]修宁宁,郑碧英,辛青松.2013年东莞市沙门菌感染流行病学特征及耐药性分析[J].中华临床医师杂志,2015,13(9):2530-2534.
[11]林金丽,梁玉潮,梁卫桥,等.公共场所从业人员沙门氏菌携带情况分析[J].当代医学,2013,19(23):160-161.
[12]杨晓,姜云,庞作章.大型活动饮食服务人员肠道致病菌检查结果分析[J].职业与健康,2013,29(16):2020-2024.
[13]陈延秋,廖亦红,曹春远.2006-2008年龙岩市新罗区从业人员体检结果分析[J].职业与健康,2010,26(6):661-662.
[14]张新,王全意,曲梅,等.2009-2011年北京市腹泻儿童沙门菌感染流行病学特征和耐药分析[J].中国病原生物学杂志,2012,7(10):728-730.
[15]宿飞,沈福杰,王怡珺,等.上海市黄浦区2008-2011年沙门菌感染流行病学分析[J].上海预防医学,2013,25(1):4-6.
[16]付秀影,黄露.一起鼠伤寒沙门氏菌食物中毒的流行病学调查[J].首都公共卫生,2016,10(2):86-89.