基于HPLC指纹图谱的当归不同干燥品质量研究
|
摘要
目的:建立当归HPLC指纹图谱,对当归不同干燥品进行质量评价。方法:对不同干燥方法得到的当归药材进行HPLC指纹图谱检测,采用TC-C_(18)(2)色谱柱(250 mm×4.6 mm,5μm),乙腈-1%乙酸水溶液梯度洗脱,流速0.6 mL·min~(-1),检测波长280 nm,柱温30℃,采用相似度评价、主成分分析和聚类分析方法对当归不同干燥品的指纹图谱进行分析。结果:建立了当归药材HPLC指纹图谱,确定了24个共有峰,指认了4个峰,14种干燥方法得到的当归样品与对照图谱相似度为0.920～0.993;主成分分析排名靠前的样品有60℃微波干燥、减压干燥、60℃热泵干燥品;聚类分析将熏干品和其他干燥品分开。结论:所建立的HPLC指纹图谱方法准确、可靠,可为当归药材质量的综合评价提供参考依据。
Objective:To establish an HPLC fingerprint of Angelicae Sinensis Radix(ASR),as well as evaluate the quality of ASR by different drying methods.Methods:Agilent 1100 was applied to analyze different drying products of Angelica sinensis,TC-C_(18)(2)column(250 mm×4.6 mm,5 μm)was performed with acetonitrile-1% acetic acid in a gradient mode at a flow rate of 0.6 mL·min~(-1) at 280 nm and the separation was performed at 30 ℃.In addition,the similarity analysis,principal component analysis(PCA),and cluster analysis(CA)were used to analyze the fingerprints of different samples.Results:Based on Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica,the common fingerprints were established,among which 24 peaks were determined as the common fingerprint peaks,and 4 of them were identified by comparing with reference substances.The similarity of ASR was in the range of 0.920～0.993.The ranking was obtained by PCA.All samples were classified into two categories by CA.Conclusion:The HPLC fingerprint method is simple and reliable,which can provide a reference for the comprehensive quality evaluation of Angelicae Sinensis Radix.
Objective:To establish an HPLC fingerprint of Angelicae Sinensis Radix(ASR),as well as evaluate the quality of ASR by different drying methods.Methods:Agilent 1100 was applied to analyze different drying products of Angelica sinensis,TC-C_(18)(2)column(250 mm×4.6 mm,5 μm)was performed with acetonitrile-1% acetic acid in a gradient mode at a flow rate of 0.6 mL·min~(-1) at 280 nm and the separation was performed at 30 ℃.In addition,the similarity analysis,principal component analysis(PCA),and cluster analysis(CA)were used to analyze the fingerprints of different samples.Results:Based on Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica,the common fingerprints were established,among which 24 peaks were determined as the common fingerprint peaks,and 4 of them were identified by comparing with reference substances.The similarity of ASR was in the range of 0.920～0.993.The ranking was obtained by PCA.All samples were classified into two categories by CA.Conclusion:The HPLC fingerprint method is simple and reliable,which can provide a reference for the comprehensive quality evaluation of Angelicae Sinensis Radix.
引文
[1] 国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:133.
[2] YI L Z,LIANG Y Z,WU H,et al.The analysis of Radix Angelicae Sinensis(Danggui)[J].J Chromatogr A,2009,1261(11):1991-2001.
[3] 张来宾,吕洁丽,陈红丽,等.当归中苯酞类成分及其药理作用研究进展[J].中国中药杂志,2016,41(2):167-176.
[4] 李文涛,杨世海.当归质量评价研究现状[J].药物分析杂志,2013,33(3):517-523.
[5] LI X N,CHEN Y Y,CHENG D P,et al.Two phthalide dimers from the radix of Angelica sinensis[J].Nat Prod Res,2012,26(19):1782.
[6] 李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023-1028.
[7] 孙国祥,胡玥珊,智雪枝.用复杂性科学原理揭示中药指纹图谱的本质特征[J].中南药学,2008,6(5):600-605.
[8] 李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[9] 谢培山.刍议中药指纹图谱的现状、发展和问题[J].中药材,2007,30(3):257-259.
[10] 赵润怀,段金廒,高振江,等.中药材产地加工过程传统与现代干燥技术方法的分析评价[J].中国现代中药,2013,15(12):1026.
[11] 陈林伟,秦昆明,朱艳汇,等.中药材产地加工的研究现状及展望[J].中国中药杂志,2015,40(4):602-606.
[12] 李硕.当归不同栽培品种种子与药材质量的相关性研究[D].成都:成都中医药大学,2015:96-97.
[13] 王龙,李军.不同干燥工艺对当归中有效成分的含量影响[J].现代医学与健康研究电子杂志,2017,1(4):157-158.
[14] 钱怡云.采收加工贮藏方法对当归药材质量的影响及其定量分析模式研究[D].兰州:甘肃中医学院,2014:5-12.
[2] YI L Z,LIANG Y Z,WU H,et al.The analysis of Radix Angelicae Sinensis(Danggui)[J].J Chromatogr A,2009,1261(11):1991-2001.
[3] 张来宾,吕洁丽,陈红丽,等.当归中苯酞类成分及其药理作用研究进展[J].中国中药杂志,2016,41(2):167-176.
[4] 李文涛,杨世海.当归质量评价研究现状[J].药物分析杂志,2013,33(3):517-523.
[5] LI X N,CHEN Y Y,CHENG D P,et al.Two phthalide dimers from the radix of Angelica sinensis[J].Nat Prod Res,2012,26(19):1782.
[6] 李曦,张丽宏,王晓晓,等.当归化学成分及药理作用研究进展[J].中药材,2013,36(6):1023-1028.
[7] 孙国祥,胡玥珊,智雪枝.用复杂性科学原理揭示中药指纹图谱的本质特征[J].中南药学,2008,6(5):600-605.
[8] 李强,杜思邈,张忠亮,等.中药指纹图谱技术进展及未来发展方向展望[J].中草药,2013,44(22):3095-3104.
[9] 谢培山.刍议中药指纹图谱的现状、发展和问题[J].中药材,2007,30(3):257-259.
[10] 赵润怀,段金廒,高振江,等.中药材产地加工过程传统与现代干燥技术方法的分析评价[J].中国现代中药,2013,15(12):1026.
[11] 陈林伟,秦昆明,朱艳汇,等.中药材产地加工的研究现状及展望[J].中国中药杂志,2015,40(4):602-606.
[12] 李硕.当归不同栽培品种种子与药材质量的相关性研究[D].成都:成都中医药大学,2015:96-97.
[13] 王龙,李军.不同干燥工艺对当归中有效成分的含量影响[J].现代医学与健康研究电子杂志,2017,1(4):157-158.
[14] 钱怡云.采收加工贮藏方法对当归药材质量的影响及其定量分析模式研究[D].兰州:甘肃中医学院,2014:5-12.
© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号 地址：北京市海淀区学院路29号 邮编：100083 电话：办公室：(+86 10)66554848；文献借阅、咨询服务、科技查新：66554700 |