ATS藻板与自然礁石上藻群生物多样性的比较
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摘要
ATS(algalturfscrubber)系统是一种利用潮间带藻类高效去除富营养化水体中氮(N)、磷(P)元素的水质净化系统,藻类是支持整个系统最重要的组成部分,其群落结构与水质净化效果密切相关。该研究基于Illumina MiSeq平台的高通量测序技术对海水水族缸的ATS系统中藻板样品和自然礁石上的藻类进行多样性分析。18S rDNA研究结果表明, ATS系统中经筛选得到藻类优化序列21390条,其中对丝藻属Antithamnion占主要地位,占序列总数的22.79%,其次19.07%序列归入斜纹藻属Psammodictyon;宋井、展南亭、后宅3个地区的藻类经筛选分别得到88435、87129、106458条优化序列,主要为分枝色指藻属Chroodactylon、宽果藻属Mastocarpus和日本马泽藻属Mazzaella。ATS系统和自然环境样品共有的优势藻为骨条藻属Skeletonema和仙菜属Ceramium。本实验在研究ATS系统与自然礁石上的藻类群落相似性的同时,深入探究了其群落结构的差异性,为进一步优化ATS系统的功能提供了理论依据。
Algal turf scrubber(ATS) is a system for water cleaning, which uses intertidal algae to effectively eliminate nitrogen(N) and phosphorus(P) elements in eutrophic water. Algae is the most important organism in the whole system, and its community structure is closely related to the effect of water purification. Based on the high-throughput sequencing technology of Illumina MiSeq platform, we analyze the diversity of algal samples in ATS system and natural reefs in this study. The results of 18 S rDNA show that the ATS system obtains 21390 algal majorizing sequences. Among these majorizing sequences, Antithamnion accounts for 22.79% of the total number, and the rest sequences are classified as Psammodictyon, accounting for 19.07%. Algae in Song Jing, Zhan Nanting and Hou Zhai areas have 88435, 87129 and 106458 majorizing sequences after filtering, respectively. They are mainly Chroodactylon, Mastocarpus and Mazzaella. Both the ATS system and the natural environment samples have the dominant species of Skeletonema and Ceramium. This research shows that the ATS system has similarity and specificity in its community structure compared to the natural reefs, which provides a theoretical evidence for further improving the function of the ATS system.
Algal turf scrubber(ATS) is a system for water cleaning, which uses intertidal algae to effectively eliminate nitrogen(N) and phosphorus(P) elements in eutrophic water. Algae is the most important organism in the whole system, and its community structure is closely related to the effect of water purification. Based on the high-throughput sequencing technology of Illumina MiSeq platform, we analyze the diversity of algal samples in ATS system and natural reefs in this study. The results of 18 S rDNA show that the ATS system obtains 21390 algal majorizing sequences. Among these majorizing sequences, Antithamnion accounts for 22.79% of the total number, and the rest sequences are classified as Psammodictyon, accounting for 19.07%. Algae in Song Jing, Zhan Nanting and Hou Zhai areas have 88435, 87129 and 106458 majorizing sequences after filtering, respectively. They are mainly Chroodactylon, Mastocarpus and Mazzaella. Both the ATS system and the natural environment samples have the dominant species of Skeletonema and Ceramium. This research shows that the ATS system has similarity and specificity in its community structure compared to the natural reefs, which provides a theoretical evidence for further improving the function of the ATS system.
引文
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刘京,胡章立,雷安平,2005.藻类快速鉴定研究进展[J].生物技术通讯,16(6):700-702.LIU JING,HU ZHANGLI,LEIANPING,2005.The advances in identification for the algae[J].Letters in Biotechnology,16(6):700-702(in Chinese with English abstract).
苗祯,罗玮,杜宗军,等,2016.北极海域优势种微单胞藻(Micromonas sp.)与其关联菌群的相互作用[J].极地研究,28(4):474-483.MIAO ZHEN,LUO WEI,DU ZONGJUN,et al,2016.Interactions between Micromonas sp.and associated bacteria in the Arctic Sea[J].Chinese Journal of Polar Research,28(4):474-483(in Chinese with English abstract).
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沈梅丽,杨锐,骆其君,等,2013.坛紫菜养殖周期中的藻际微生物多样性[J].微生物学报,53(10):1087-1102.SHENMEILI,YANG RUI,LUO QIJUN,et al,2013.Microbial diversity of Pyropia haitanensis phycosphere during cultivation[J].Acta Microbiologica Sinica,53(10):1087-1102(in Chinese with English abstract).
王翔宇,詹冬梅,李美真,等,2011.大型海藻吸收氮磷营养盐能力的初步研究[J].渔业科学进展,32(4):67-71.WANGXIANGYU,ZHAN DONGMEI,LI MEIZHEN,et al,2011.Preliminary studies on the nitrogen and phosphorus absorption capability of macroalgae[J].Progress in Fishery Sciences,32(4):67-71(in Chinese with English abstract).
叶志娟,2006.牟氏角毛藻和盐藻对不同种类海水养殖废水生长和生理反应的研究[D].南京:南京农业大学:1-62.YE ZHIJUAN,2006.Growth and physiological responses of Chaetoceros Mulleri and Dunaliella Salina to different aquaculture wastewater[D].Nanjing:Nanjing Agricultural University:1-62(in Chinese with English abstract).
张艳敏,王江涛,谭丽菊,2017.海水中藻菌共培养体系对碳氮磷的吸收转化[J].生态学报,37(14):4843-4851.ZHANGYANMIN,WANG JIANGTAO,TAN LIJU,2017.Uptake and transformation of carbon,nitrogen and phosphorus in the co-culture system of algae and bacteria in seawater[J].Acta Ecologica Sinica,37(14):4843-4851(in Chinese with English abstract).
赵以军,刘永定,1996.有害藻类及其微生物防治的基础--藻菌关系的研究动态[J].水生生物学报,20(2):173-181.ZHAO YIJUN,LIU YONGDING,1996.Possible microbial control on the adverse impacts of algae-current information about the relationship between algae and microbes[J].Acta Hydrobiologica Sinica,20(2):173-181(in Chinese with English abstract).
ADEY W H,1982-06-08.Algal turf scrubber:US,4333263[P].
GU XINGYAN,LI KEQIANG,PANG KAI,et al,2017.Effects of p H on the growth and NH4-N uptake of Skeletonema costatum and Nitzschia closterium[J].Marine Pollution Bulletin,124(2):946-952.
LICHTLéC,1980.Effects of nitrogen deficiency and light of high intensity on Cryptomonas rufescens(Cryptophyceae):II.Excystment[J].Protoplasma,102(1-2):11-19.
NOVOVESKáL,ZAPATA A K M,ZABOLOTNEY J B,et al,2016.Optimizing microalgae cultivation and wastewater treatment in large-scale offshore photobioreactors[J].Algal Research,18:86-94.
李恒,李美真,徐志广,等,2012.不同营养盐浓度对3种大型红藻氮、磷吸收及其生长的影响[J].中国水产科学,19(3):462-470.LI HENG,LI MEIZHEN,XU ZHIGUANG,et al,2012.Effect of nutrient supply on nitrogen and phosphorus uptake and growth in three species of macroalgae[J].Journal of Fishery Sciences of China,19(3):462-470(in Chinese with English abstract).
李克亚,文章,邓斌,等,2016.不同窖龄窖泥原核生物多样性的高通量测序研究[J].食品工业,37(6):121-125.LI KEYA,WEN ZHANG,DENG BIN,et al,2016.High-throughput sequencing using in research for community diversity of prokaryotes in pit mud of different ages[J].The Food Industry,37(6):121-125(in Chinese with English abstract).
刘京,胡章立,雷安平,2005.藻类快速鉴定研究进展[J].生物技术通讯,16(6):700-702.LIU JING,HU ZHANGLI,LEIANPING,2005.The advances in identification for the algae[J].Letters in Biotechnology,16(6):700-702(in Chinese with English abstract).
苗祯,罗玮,杜宗军,等,2016.北极海域优势种微单胞藻(Micromonas sp.)与其关联菌群的相互作用[J].极地研究,28(4):474-483.MIAO ZHEN,LUO WEI,DU ZONGJUN,et al,2016.Interactions between Micromonas sp.and associated bacteria in the Arctic Sea[J].Chinese Journal of Polar Research,28(4):474-483(in Chinese with English abstract).
单乐州,邵鑫斌,吴洪喜,2014.ATS在海水工厂化循环水生态养殖中的应用[J].水产养殖,(9):4-6(in Chinese).
单乐州,邵鑫斌,曹醒戈,2016.小丑鱼室内循环水养殖设施与技术[J].渔业现代化,43(2):7-10.SHAN LEZHOU,SHAOXINBING,CAO XINGGE,2016.Facilities and techniques for indoor recirculating aquaculture of clownfish[J].Fishery Modernization,43(2):7-10(in Chinese with English abstract).
沈梅丽,杨锐,骆其君,等,2013.坛紫菜养殖周期中的藻际微生物多样性[J].微生物学报,53(10):1087-1102.SHENMEILI,YANG RUI,LUO QIJUN,et al,2013.Microbial diversity of Pyropia haitanensis phycosphere during cultivation[J].Acta Microbiologica Sinica,53(10):1087-1102(in Chinese with English abstract).
王翔宇,詹冬梅,李美真,等,2011.大型海藻吸收氮磷营养盐能力的初步研究[J].渔业科学进展,32(4):67-71.WANGXIANGYU,ZHAN DONGMEI,LI MEIZHEN,et al,2011.Preliminary studies on the nitrogen and phosphorus absorption capability of macroalgae[J].Progress in Fishery Sciences,32(4):67-71(in Chinese with English abstract).
叶志娟,2006.牟氏角毛藻和盐藻对不同种类海水养殖废水生长和生理反应的研究[D].南京:南京农业大学:1-62.YE ZHIJUAN,2006.Growth and physiological responses of Chaetoceros Mulleri and Dunaliella Salina to different aquaculture wastewater[D].Nanjing:Nanjing Agricultural University:1-62(in Chinese with English abstract).
张艳敏,王江涛,谭丽菊,2017.海水中藻菌共培养体系对碳氮磷的吸收转化[J].生态学报,37(14):4843-4851.ZHANGYANMIN,WANG JIANGTAO,TAN LIJU,2017.Uptake and transformation of carbon,nitrogen and phosphorus in the co-culture system of algae and bacteria in seawater[J].Acta Ecologica Sinica,37(14):4843-4851(in Chinese with English abstract).
赵以军,刘永定,1996.有害藻类及其微生物防治的基础--藻菌关系的研究动态[J].水生生物学报,20(2):173-181.ZHAO YIJUN,LIU YONGDING,1996.Possible microbial control on the adverse impacts of algae-current information about the relationship between algae and microbes[J].Acta Hydrobiologica Sinica,20(2):173-181(in Chinese with English abstract).
ADEY W H,1982-06-08.Algal turf scrubber:US,4333263[P].
GU XINGYAN,LI KEQIANG,PANG KAI,et al,2017.Effects of p H on the growth and NH4-N uptake of Skeletonema costatum and Nitzschia closterium[J].Marine Pollution Bulletin,124(2):946-952.
LICHTLéC,1980.Effects of nitrogen deficiency and light of high intensity on Cryptomonas rufescens(Cryptophyceae):II.Excystment[J].Protoplasma,102(1-2):11-19.
NOVOVESKáL,ZAPATA A K M,ZABOLOTNEY J B,et al,2016.Optimizing microalgae cultivation and wastewater treatment in large-scale offshore photobioreactors[J].Algal Research,18:86-94.
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