应用mtDNA序列分析技术鉴定嗜尸性蝇类种属的研究进展
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摘要
随着法医昆虫学的不断发展,应用嗜尸性昆虫的生长发育规律和尸体上昆虫演替规律推断死亡时间成为可能,但这要求快速、准确地鉴别嗜尸性昆虫的种属,然而,办案人员通常无法从形态上鉴别昆虫幼虫的种属,而对成虫的形态学种属鉴定知识也往往所知甚少,因此限制了相关技术在案件侦破中的广泛应用。自1994年sperling利用mtDNA序列分析方法进行种属鉴定以来,国内外许多研究者在此基础上进行了大量的尝试与探索,特别是近十几年,国内研究者在借鉴国外研究成果的基础上,在全国不同地域采集蝇类标本进行序列分析研究,在提取方法、基因片段种属鉴定效果、种属鉴定应用等方面取得了较大的研究成果。本文试图对国内近几年的研究进行总结,从而为后续研究提供参考。
Forensic entomology can provide clues for investigators to estimate the postmortem interval based on the growing and developing patterns of sarcosaphagous pupas showing their metamorphous evolutions on the corpses. However, only a few experts are able to master how to identify the species of sarcosaphagous pupas or flies using the morphology knowledge, thereby limiting the application of such entomologic capability into criminal detection. Fortunately, a large number of attempts both home and abroad have been explored to authenticate the sarcosaphagous pupas or flies with their mtDNA(mitochondrial DNA) sequencing since Sperling made such a kind of identification in 1994, having obtained plenty of achievements. Domestic researchers also acquired great progress on the aspects of collecting samples, evaluating the species identification through sequencing the relevant gene fragments of mtDNA, applying the method of species identification at the basis of gathering various flies specimens in different regions nationwide, and analyzing the flies' targeted mtDNA sequences. This review intends to make a summary about the latest domestic researches on this tendency.
Forensic entomology can provide clues for investigators to estimate the postmortem interval based on the growing and developing patterns of sarcosaphagous pupas showing their metamorphous evolutions on the corpses. However, only a few experts are able to master how to identify the species of sarcosaphagous pupas or flies using the morphology knowledge, thereby limiting the application of such entomologic capability into criminal detection. Fortunately, a large number of attempts both home and abroad have been explored to authenticate the sarcosaphagous pupas or flies with their mtDNA(mitochondrial DNA) sequencing since Sperling made such a kind of identification in 1994, having obtained plenty of achievements. Domestic researchers also acquired great progress on the aspects of collecting samples, evaluating the species identification through sequencing the relevant gene fragments of mtDNA, applying the method of species identification at the basis of gathering various flies specimens in different regions nationwide, and analyzing the flies' targeted mtDNA sequences. This review intends to make a summary about the latest domestic researches on this tendency.
引文
[1]徐长苗,程建波,王江峰,等.法医昆虫学在死亡时间推断中应用3例[J].中国法医学杂志,1999,14(3):172-73.
[2]王江峰,陈玉川,竞花兰,等.法医昆虫学的发展及应用[J].中国法医学杂志,2004,19(S):19-20.
[3]SPERLING FAH,ANDERSON G S,HICKEU D A.A DNA-based approach to the identification of insect specimens used for postmortem interval estimation[J].Journal of Forensic Sci-ence.1994,39:418-427.
[4]胡萃.法医昆虫学[M].重庆:重庆出版社,2000:8-11.
[5]陈瑶清,郭亚东,李茂枝,等.常见嗜尸性昆虫mtDNA提取方法的比较[J].法医学杂志,2011,27(4):265-270.
[6]郭亚东,蔡继峰,苏日娜,等.常见嗜尸性甲虫及蝇类虫体不同部位mtDNA的提取效果[J].法医学杂志,2010,26(5):336-339.
[7]李学博,宁淑华,张贵芹,等.常见嗜尸性蝇类蛹壳样品在种属分子鉴定中的应用[J].中国寄生虫学与寄生虫病杂志,2015,33(1):35-39.
[8]李响,蔡继峰.mtDNA多态性在嗜尸性昆虫种类鉴定中的应用[J].法医学杂志,2011,27(2):133-138.
[9]蔡继峰,董建国,刘敏,等.呼和浩特等地区嗜尸性苍蝇mtDNA中CO I基因序列检测及法医学应用[J].法医学杂志,2005,25(2):100-106.
[10]蔡继峰,刘敏,应斌武,等.成都地区四种食尸性蝇类mtDNA中COI基因序列检测[J].昆虫学报,2005,48(1):101-106.
[11]王江峰,尹晓宏,陈玉川.五种常见嗜尸性蝇类的分子鉴定[J].昆虫学报,2007,50(4):423-428.
[12]王新杰,王学海,刁立江,等.潍坊地区六种常见嗜尸性蝇类mtDNA CO I区序列研究[J].法医学杂志,2006,22:93-94.
[13]杨静波,姜建军,王江峰.mtDNA CO I和ND5基因用于鉴别常见嗜尸性蝇类[J].中国法医学杂志,2012,27(3):201-204.
[14]汤振楚,郭亚东,蔡继峰,等.应用CO I短序列片段鉴定常见嗜尸性麻蝇[J].中南大学学报(医学版),2012,37(1):1183-1187.
[15]杨立,蔡继峰,文继舫,等.中国14省不同地区常见嗜尸性苍蝇mtDNA中CO I基因序列检测.中南大学学报(医学版),2010,35(8):819-825.
[16]陈庆,白洁,刘力,等.北京地区7种常见嗜尸性蝇类的COⅠ基因序列分析及DNA条形码的建立[J].昆虫学报,2009,52(2):202-209.
[17]CATERINO M S,CHO S,SPERLING F A.The current state of insect molecular systematics:a thriving Tower of Babel[J].Annual Review of Entomology,2000,45:1-54.
[18]蔡继峰,常云峰,廖志钢,等.嗜尸性苍蝇mtDNA中COⅡ基因序列检测[J].中国法医学杂志,2005,20(4):193-195.
[19]赵祖亮,闫红涛,李世才,等.郑州地区食尸性苍蝇及幼虫mtDNA中COII基因序列分析[J].法医学杂志,2010,26(2),104-107.
[20]蔡继峰,兰玲梅,陈瑶清,等.丝光绿蝇CO II序列分析与地理种群分布的关系[J].法医学杂志,2006,22(6):401-403.
[21]RICHARD Z,JENS A,SVENJA S et al.Genetic identification of forensically important flesh flies(Diptera:Sarcophagidae)[J].International Journal of Legal Medicine,2004,118:245-247.
[22]江世宏,孟子烨,陈晓琴,等.核糖体DNA序列分析在昆虫系统学研究中的应用[J].昆虫分类学报,2008,30(3):225-238.
[23]孙晓明,蔡继峰,应斌武,等.16S rDNA序列分析在嗜尸性蝇类鉴定中的应用[J].法医学杂志,2006,22(1):36-38.
[24]石坚,郭亚东,匡栩源,等.应用16S rDNA短序列片段鉴定常见嗜尸性丽蝇[J].法医学杂志,2012,28(4):281-285.
[25]陈凤磊,蔡继峰,郭亚东,等.利用16S rDNA序列鉴定常见嗜尸性蝇类种属[J].中国法医学杂志,2011,26(5):362-364.
[26]LI X,CAI J F,GUO Y D,et al.The availability of 16S rRNAfor the identification of forensically important flies(Diptera:Muscidae)in China[J].Tropical Biomedicine,2010,27(2):155-166.
[27]张见影,伦志军,李正红.NCBI基因序列数据库使用和检索方法[J].现代情报,2003,23(12):224-225.
[28]李学博,刘东,巩强,等.NCBI数据库在常见嗜尸性蝇类种属鉴别中的应用[J].中国法医学杂志,2014,29(1):11-14.
[29]AGRA M F,FRACNA P F,BARBOSA-FILHO J M.Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil[J].Rev Bras Farmacogn,2007,17:114-140.
[30]张红玲,杨明,王杰,等.贵阳地区常见嗜尸性蝇类丽蝇科DNA条形码的构建[J].贵阳医学院学报,2012,37(5):490-496.
[31]HEBERT P D N,CYWINSKA A,BALL S L,et a1.Biological identifications through DNA barcodes[J].Proc R Soc London(Series B),2003,270:313-321.
[2]王江峰,陈玉川,竞花兰,等.法医昆虫学的发展及应用[J].中国法医学杂志,2004,19(S):19-20.
[3]SPERLING FAH,ANDERSON G S,HICKEU D A.A DNA-based approach to the identification of insect specimens used for postmortem interval estimation[J].Journal of Forensic Sci-ence.1994,39:418-427.
[4]胡萃.法医昆虫学[M].重庆:重庆出版社,2000:8-11.
[5]陈瑶清,郭亚东,李茂枝,等.常见嗜尸性昆虫mtDNA提取方法的比较[J].法医学杂志,2011,27(4):265-270.
[6]郭亚东,蔡继峰,苏日娜,等.常见嗜尸性甲虫及蝇类虫体不同部位mtDNA的提取效果[J].法医学杂志,2010,26(5):336-339.
[7]李学博,宁淑华,张贵芹,等.常见嗜尸性蝇类蛹壳样品在种属分子鉴定中的应用[J].中国寄生虫学与寄生虫病杂志,2015,33(1):35-39.
[8]李响,蔡继峰.mtDNA多态性在嗜尸性昆虫种类鉴定中的应用[J].法医学杂志,2011,27(2):133-138.
[9]蔡继峰,董建国,刘敏,等.呼和浩特等地区嗜尸性苍蝇mtDNA中CO I基因序列检测及法医学应用[J].法医学杂志,2005,25(2):100-106.
[10]蔡继峰,刘敏,应斌武,等.成都地区四种食尸性蝇类mtDNA中COI基因序列检测[J].昆虫学报,2005,48(1):101-106.
[11]王江峰,尹晓宏,陈玉川.五种常见嗜尸性蝇类的分子鉴定[J].昆虫学报,2007,50(4):423-428.
[12]王新杰,王学海,刁立江,等.潍坊地区六种常见嗜尸性蝇类mtDNA CO I区序列研究[J].法医学杂志,2006,22:93-94.
[13]杨静波,姜建军,王江峰.mtDNA CO I和ND5基因用于鉴别常见嗜尸性蝇类[J].中国法医学杂志,2012,27(3):201-204.
[14]汤振楚,郭亚东,蔡继峰,等.应用CO I短序列片段鉴定常见嗜尸性麻蝇[J].中南大学学报(医学版),2012,37(1):1183-1187.
[15]杨立,蔡继峰,文继舫,等.中国14省不同地区常见嗜尸性苍蝇mtDNA中CO I基因序列检测.中南大学学报(医学版),2010,35(8):819-825.
[16]陈庆,白洁,刘力,等.北京地区7种常见嗜尸性蝇类的COⅠ基因序列分析及DNA条形码的建立[J].昆虫学报,2009,52(2):202-209.
[17]CATERINO M S,CHO S,SPERLING F A.The current state of insect molecular systematics:a thriving Tower of Babel[J].Annual Review of Entomology,2000,45:1-54.
[18]蔡继峰,常云峰,廖志钢,等.嗜尸性苍蝇mtDNA中COⅡ基因序列检测[J].中国法医学杂志,2005,20(4):193-195.
[19]赵祖亮,闫红涛,李世才,等.郑州地区食尸性苍蝇及幼虫mtDNA中COII基因序列分析[J].法医学杂志,2010,26(2),104-107.
[20]蔡继峰,兰玲梅,陈瑶清,等.丝光绿蝇CO II序列分析与地理种群分布的关系[J].法医学杂志,2006,22(6):401-403.
[21]RICHARD Z,JENS A,SVENJA S et al.Genetic identification of forensically important flesh flies(Diptera:Sarcophagidae)[J].International Journal of Legal Medicine,2004,118:245-247.
[22]江世宏,孟子烨,陈晓琴,等.核糖体DNA序列分析在昆虫系统学研究中的应用[J].昆虫分类学报,2008,30(3):225-238.
[23]孙晓明,蔡继峰,应斌武,等.16S rDNA序列分析在嗜尸性蝇类鉴定中的应用[J].法医学杂志,2006,22(1):36-38.
[24]石坚,郭亚东,匡栩源,等.应用16S rDNA短序列片段鉴定常见嗜尸性丽蝇[J].法医学杂志,2012,28(4):281-285.
[25]陈凤磊,蔡继峰,郭亚东,等.利用16S rDNA序列鉴定常见嗜尸性蝇类种属[J].中国法医学杂志,2011,26(5):362-364.
[26]LI X,CAI J F,GUO Y D,et al.The availability of 16S rRNAfor the identification of forensically important flies(Diptera:Muscidae)in China[J].Tropical Biomedicine,2010,27(2):155-166.
[27]张见影,伦志军,李正红.NCBI基因序列数据库使用和检索方法[J].现代情报,2003,23(12):224-225.
[28]李学博,刘东,巩强,等.NCBI数据库在常见嗜尸性蝇类种属鉴别中的应用[J].中国法医学杂志,2014,29(1):11-14.
[29]AGRA M F,FRACNA P F,BARBOSA-FILHO J M.Synopsis of the plants known as medicinal and poisonous in Northeast of Brazil[J].Rev Bras Farmacogn,2007,17:114-140.
[30]张红玲,杨明,王杰,等.贵阳地区常见嗜尸性蝇类丽蝇科DNA条形码的构建[J].贵阳医学院学报,2012,37(5):490-496.
[31]HEBERT P D N,CYWINSKA A,BALL S L,et a1.Biological identifications through DNA barcodes[J].Proc R Soc London(Series B),2003,270:313-321.