外源亚精胺对高温胁迫下生菜幼苗生长及抗氧化酶活性的影响
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摘要
【目的】为确定生菜喷施外源亚精胺(Spd)适宜的浓度,寻找外源Spd缓解胁迫伤害以及生菜栽培提供理论依据。【方法】以耐高温品种G-S59和不耐高温品种P-S11为试验材料,分别喷施0.1、0.5、1.0、1.5和2.0mM的外源亚精胺,并以喷施去离子水为对照,测定了不同浓度外源亚精胺对高温胁迫下生菜的生长指标、叶绿素含量、抗氧化酶活性、膜透性和根系活力。【结果】随着喷施Spd浓度的提高,2个品种的生菜植株生长量、根系活力、叶绿素含量、超氧化物歧化酶、过氧化氢酶和过氧化物酶活性均呈先增加后减少的趋势,且在喷施1.0mM Spd处理达到峰值;外源喷施亚精胺有效降低了膜透性和丙二醛含量,在亚精胺浓度为1.0mM时,2个生菜品种的电解质渗透率和丙二醛含量均达到最低值。【结论】生菜在高温胁迫下,外源喷施亚精胺的适宜浓度为1.0mM。
【Objective】Lettuce is not resistant to high temperatures.When the temperature is too high,it will be spindling and early bolting.The effects of Exogenous Spermidine on the growth of lettuce seedlings and the heat tolerance of antioxidant enzyme system were studied.【Methods】The exogenous spermidines of 0.1,0.5,1.0,1.5 and 2.0 mM were sprayed on high temperature tolerant varieties G-S59 and P-S11 respectively.The growth index,chlorophyll content,antioxidant enzyme activity,membrane permeability and root activity of lettuce under high temperature stress were determined by spraying deionized water as control.【Results】With the increasing of spermidine(Spd)concentration,plant growth,superoxide dismutase,catalase and peroxidase activities of two varieties lettuce increased beginning and then decreasing,and reached the peak value in 1.0 mM Spd treatment.Exogenous spermidine spraying reduced membrane permeability effectively.The electrolyte permeability and malondialdehyde contents of two lettuce varieties achieved the lowest values at spermidine concentration of 1.0 mM.【Conclusion】Under high temperature stress of lettuce,the suitable concentration of exogenous spray spermidine was 1.0 mM.
【Objective】Lettuce is not resistant to high temperatures.When the temperature is too high,it will be spindling and early bolting.The effects of Exogenous Spermidine on the growth of lettuce seedlings and the heat tolerance of antioxidant enzyme system were studied.【Methods】The exogenous spermidines of 0.1,0.5,1.0,1.5 and 2.0 mM were sprayed on high temperature tolerant varieties G-S59 and P-S11 respectively.The growth index,chlorophyll content,antioxidant enzyme activity,membrane permeability and root activity of lettuce under high temperature stress were determined by spraying deionized water as control.【Results】With the increasing of spermidine(Spd)concentration,plant growth,superoxide dismutase,catalase and peroxidase activities of two varieties lettuce increased beginning and then decreasing,and reached the peak value in 1.0 mM Spd treatment.Exogenous spermidine spraying reduced membrane permeability effectively.The electrolyte permeability and malondialdehyde contents of two lettuce varieties achieved the lowest values at spermidine concentration of 1.0 mM.【Conclusion】Under high temperature stress of lettuce,the suitable concentration of exogenous spray spermidine was 1.0 mM.
引文
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[12]李丽杰,顾万荣,孟瑶,等.干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制[J].应用生态学报,2018,29(2):554-564
[13]冀保毅,李杰,信龙飞,等.亚精胺对水分胁迫下稻茬小麦幼苗抗氧化特性及根系活力的影响[J].河南农业科学,2018,47(3):29-33
[14]Mostofa M G,Yoshida N,Fujita M.Spermidine pretreatment enhances heat tolerance in rice seedlings through modulating antioxidative and glyoxalase systems[J].Plant Growth Regulation,2014,73(1):31-44
[15]潘媛媛,张毅,石玉,等.外源亚精胺对等渗盐胁迫下番茄幼苗抗氧化系统的影响[J].西北农林科技大学学报(自然科学版),2019,47(3):1-9
[16]周珩.外源亚精胺缓解高温胁迫下黄瓜幼苗叶绿素代谢的作用机理研究[D].南京:南京农业大学,2016
[17]陈福明,陈顺伟.混合液法测定叶绿素含量的研究[J].林业科技通讯,1984(2):4-8
[18]Gong M,Li Y J,Chen S Z.Abscisic acid-induced the rmotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems[J].Journal of Plant Physiology.1998,153(4):488-496
[19]刘萍,李明军.植物生理学实验[M].第2版.北京:科学出版社,2016:142-150
[20]李小方,张志良.植物生理学实验指导[M].第5版.北京:高等教育出版社,2016:30-31
[21]Joshi R,Chinnusamy V.Oxidative Damage to Plants[M].San Diege(USA):Academic Press,2014:369-396
[22]郝婷,丁小涛,张红梅,等.不同根际温度对黄瓜幼苗生长,光合及叶绿素荧光特性的影响[J].上海农业学报,2015,31(2):45-50
[23]Sgobba A,Paradiso A,Dipierro S,et al.Changes in antioxidants are critical in determining cell responses to short‐and long‐term heat stress[J].Physiologia Plantarum,2015,153(1):68-78
[24]Gupta B,Huang B.Mechanism of salinity tolerance in plants:physiological,biochemical,and molecular characterization[J].International Journal of Genomics,2014,2014:701596
[25]Do P T,Drechsel O,Heyer A G,et al.Changes in free polyamine levels,expression of polyamine biosynthesis genes,and performance of rice cultivars under salt stress:a comparison with responses to drought[J].Frontiers in Plant Science,2014,5:182
[26]Mohammad Golam Mostofa,Namiko Yoshida,Masayuki Fujita.Spermidine pretreatment enhances heat tolerance in rice seedling sthrough modulating antioxidative and glyoxalase systems[J].Plant Growth Regul,2014,73(1):31-44
[27]刘凯歌,宋云鹏,龚繁荣,等.高温胁迫对甜椒幼苗生长和生理生化指标的影响[J].上海农业学报,2015,31(3):63-67
[2]李莉,赵丽丽,钟华,等.高温胁迫对多年生黑麦草种苗特性的影响[J].安徽农业科学,2018,46(26):85-86
[3]Choudhury F K,Rivero R M,Blumwald E,et al.Reactive oxygen species,abiotic stress and stress combination[J].The Plant Journal,2017,90(5):856-867
[4]杨舒贻,陈晓阳,惠文凯,等.逆境胁迫下植物抗氧化酶系统响应研究进展[J].福建农林大学学报(自然科学版),2016,45(5):481-489
[5]Jajic I,Sarna T,Strzalka K.Senescence,stress,and reactive oxygen species[J].Plants,2015,4(3):393-411
[6]张丽,徐志然,胡晓辉,等.叶面喷施亚精胺对盐碱胁迫下番茄幼苗生长及其叶绿素合成前体含量的影响[J].西北植物学报,2015,35(1):125-130
[7]杜红阳,程明明,杨青华,等.外源亚精胺对涝胁迫下玉米幼苗根系无氧呼吸代谢的调控效应[J].华北农学报,2015,30(4):110-116
[8]李州,彭燕,潘明洪,等.亚精胺对PEG胁迫下白三叶幼苗渗透调节物质积累的影响[J].中国草地学报,2014,36(1):31-36
[9]姜秀梅,秦勇,郭光照,等.外源亚精胺对低温胁迫下辣椒种子萌发及幼苗生长的影响[J].新疆农业科学,2013,50(12):2266-2273
[10]李秀,巩彪,徐坤,等.外源亚精胺对高温胁迫下生姜叶片内源激素及叶绿体超微结构的影响[J].中国农业科学,2015,48(1):120-129
[11]彭晨,苏晓琼,杜静,等.外源亚精胺对高温胁迫下黄瓜幼苗快速叶绿素荧光诱导动力学特性的影响[J].西南农业学报,2016,29(2):260-265
[12]李丽杰,顾万荣,孟瑶,等.干旱胁迫下亚精胺对玉米幼苗抗旱性影响的生理生化机制[J].应用生态学报,2018,29(2):554-564
[13]冀保毅,李杰,信龙飞,等.亚精胺对水分胁迫下稻茬小麦幼苗抗氧化特性及根系活力的影响[J].河南农业科学,2018,47(3):29-33
[14]Mostofa M G,Yoshida N,Fujita M.Spermidine pretreatment enhances heat tolerance in rice seedlings through modulating antioxidative and glyoxalase systems[J].Plant Growth Regulation,2014,73(1):31-44
[15]潘媛媛,张毅,石玉,等.外源亚精胺对等渗盐胁迫下番茄幼苗抗氧化系统的影响[J].西北农林科技大学学报(自然科学版),2019,47(3):1-9
[16]周珩.外源亚精胺缓解高温胁迫下黄瓜幼苗叶绿素代谢的作用机理研究[D].南京:南京农业大学,2016
[17]陈福明,陈顺伟.混合液法测定叶绿素含量的研究[J].林业科技通讯,1984(2):4-8
[18]Gong M,Li Y J,Chen S Z.Abscisic acid-induced the rmotolerance in maize seedlings is mediated by calcium and associated with antioxidant systems[J].Journal of Plant Physiology.1998,153(4):488-496
[19]刘萍,李明军.植物生理学实验[M].第2版.北京:科学出版社,2016:142-150
[20]李小方,张志良.植物生理学实验指导[M].第5版.北京:高等教育出版社,2016:30-31
[21]Joshi R,Chinnusamy V.Oxidative Damage to Plants[M].San Diege(USA):Academic Press,2014:369-396
[22]郝婷,丁小涛,张红梅,等.不同根际温度对黄瓜幼苗生长,光合及叶绿素荧光特性的影响[J].上海农业学报,2015,31(2):45-50
[23]Sgobba A,Paradiso A,Dipierro S,et al.Changes in antioxidants are critical in determining cell responses to short‐and long‐term heat stress[J].Physiologia Plantarum,2015,153(1):68-78
[24]Gupta B,Huang B.Mechanism of salinity tolerance in plants:physiological,biochemical,and molecular characterization[J].International Journal of Genomics,2014,2014:701596
[25]Do P T,Drechsel O,Heyer A G,et al.Changes in free polyamine levels,expression of polyamine biosynthesis genes,and performance of rice cultivars under salt stress:a comparison with responses to drought[J].Frontiers in Plant Science,2014,5:182
[26]Mohammad Golam Mostofa,Namiko Yoshida,Masayuki Fujita.Spermidine pretreatment enhances heat tolerance in rice seedling sthrough modulating antioxidative and glyoxalase systems[J].Plant Growth Regul,2014,73(1):31-44
[27]刘凯歌,宋云鹏,龚繁荣,等.高温胁迫对甜椒幼苗生长和生理生化指标的影响[J].上海农业学报,2015,31(3):63-67