红花蜂花粉多糖Ⅰ对H22-荷瘤小鼠的抑瘤作用
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摘要
目的:探讨红花蜂花粉多糖Ⅰ(PBPCⅠ)对小鼠肝癌H22实体瘤的抑制作用。方法:对肝癌H22实体瘤模型给予不同剂量的PBPCⅠ,采用ELISA法检测血清中IL-1α、IL-6、IFN-γ、TNF-α、IL-2、VEGF的含量,剥离瘤块,取脾脏及胸腺,称质量,计算抑瘤率与脏器指数。H&E染色法检测肿瘤细胞生长情况、肿瘤细胞形态及肿瘤细胞凋亡等指标。结果:与模型组相比,CTX组和PBPCⅠ低、中、高剂量组对小鼠H22肝癌的抑瘤率分别为67.93%、 33.48%、45.69%、57.86%。PBPCⅠ低、中、高剂量组能显著提高荷瘤小鼠体质量、免疫器官指数,并明显升高荷瘤小鼠血清中IFN-γ、IL-2、TNF-α含量,降低血清IL-1α、IL-6、VEGF含量。H&E染色发现不同浓度的PBPCⅠ均能使肝癌肿瘤细胞凋亡。结论:PBPCⅠ对H22荷瘤小鼠具有抑瘤作用,其作用可能与调节机体免疫功能有关。
Objective:To investigate the inhibitory effect of safflower bee pollen polysaccharide I(PBPCI) on H22 solid tumor in mice.Methods:Liver cancer H22 solid tumor model was given different doses of PBPCI. The serum levels of IL-1α,IL-6, IFN-γ, TNF-α, IL-2, VEGF were measured by ELISA. The tumor were removed,the spleen and thymus were taken and weighed. The tumor inhibition rate and organ index were calculated. H&E staining method is used as detecting tumor cell growth,morphology and apoptosis indexes.Results:Compared with model group, CTX group and PBPCⅠlow, medium and high dose on H22 liver cancer in mice, the inhibitory rate of 67.93%, 33.48%, 45.69%, 57.86%. At the same time, PBPC Ⅰ low, medium and high dose group could significantly improve the body weight and immune organ index of tumor-bearing mice, and significantly increase the serum content of IFN-γ, IL-2 and TNF-α in tumor-bearing mice, reduce the content of IL-1α, IL-6, VEGF in serum.H&E staining showed that different concentrations of PBPCI caused apoptosis of liver cancer tumor cells.Conclusion:PBPCⅠ has a significant inhibitory effect on H22 mice, the effect may be associated with the immune function.
Objective:To investigate the inhibitory effect of safflower bee pollen polysaccharide I(PBPCI) on H22 solid tumor in mice.Methods:Liver cancer H22 solid tumor model was given different doses of PBPCI. The serum levels of IL-1α,IL-6, IFN-γ, TNF-α, IL-2, VEGF were measured by ELISA. The tumor were removed,the spleen and thymus were taken and weighed. The tumor inhibition rate and organ index were calculated. H&E staining method is used as detecting tumor cell growth,morphology and apoptosis indexes.Results:Compared with model group, CTX group and PBPCⅠlow, medium and high dose on H22 liver cancer in mice, the inhibitory rate of 67.93%, 33.48%, 45.69%, 57.86%. At the same time, PBPC Ⅰ low, medium and high dose group could significantly improve the body weight and immune organ index of tumor-bearing mice, and significantly increase the serum content of IFN-γ, IL-2 and TNF-α in tumor-bearing mice, reduce the content of IL-1α, IL-6, VEGF in serum.H&E staining showed that different concentrations of PBPCI caused apoptosis of liver cancer tumor cells.Conclusion:PBPCⅠ has a significant inhibitory effect on H22 mice, the effect may be associated with the immune function.
引文
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[21] 葛唯佳,赵岩,图力古尔,等.杂色云芝酸溶性多糖对H-(22)荷瘤小鼠的抗肿瘤作用[J].西北农林科技大学学报(自然科学版),2018(1):9-13.
[22] 魏静,李金凤,温成平,等.桑黄多糖对肝癌H22荷瘤小鼠的治疗作用[J].中药材,2016,39(12):2868-2870.
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[2] 魏桂芳,徐磊,姚宏.红花的鉴定标准与药理研究[J].陕西中医,2010,31(6):736-737.
[3] 刘建涛,赵利,苏伟.蜂花粉生物活性物质的研究进展[J].食品科学,2006,27(12):909-912.
[4] 陈圣阳刘旺景曹琪娜,等.植物多糖的生物学活性研究进展[J].饲料工业,2016,37(22):60-64.
[5] 褚珊珊,左绍远.蜂花粉多糖生物活性研究进展[J].蜜蜂杂志,2018(7):8-10
[6] 李月,王昀,左绍远,等.红花蜂花粉多糖分离纯化及活性研究[J].食品科技,2017,42(12):191-195.
[7] 左绍远,林春榕,魏淑飞,等.云南产两种蜂花粉多糖对小鼠免疫功能的影响[J].时珍国医国药,2017,28(1):7-9.
[8] 左绍远,钱金栿.红花蜂花粉多糖提取工艺的优化及其抗氧化性质研究[J].湖北农业科学,2013,52(7):1631-1633.
[9] 张翠香,罗永会,徐春萍,等.蜂花粉多糖和黄铜联合提取及含量测定[J].食品研究与开发,2017,38(10):59-62.
[10] 左绍远,钱金栿.云南产红花蜂花粉多糖的分离提取及含量测定[J].时珍国医国药,2012,23(11):2765-2766.
[11] 林春榕,张翠香,左绍远.荞麦蜂花粉多糖对糖尿病大鼠血糖、血脂的影响[J].亚太传统医药,2013,9(3):7-9.
[12] 王博,张梦珊,张中玉,等,菊花粉花粉多糖的分离纯化及抗肿瘤活性研究[J].食品工业科技,2016,37(5):358-360.
[13] 吴盼盼,段元锋,徐德平,油菜蜂花粉多糖的分离鉴定及免疫活性研究[J].食品与生物技术学报,2015,34(10):1040-1044.
[14] 杨世发.泰山松花粉多糖的成分分析及其对B亚群禽白血病的免疫调理作用[D].泰安:山东农业大学,2015
[15] WANG B,DIAO Q,ZHANG Z,et al.Antitumor activity of bee pollen polysaccharides from Rosa rugosa[J].Molecular Medicine Reports,2013,7(5):1555-1558.
[16] LI X,GONG H,YANG S,et al.Pectic Bee Pollen Polysaccharide from Rosa rugosa Alleviates Diet-Induced Hepatic Steatosis and Insulin Resistance via Induction of AMPK/mTOR-Mediated Autophagy[J].Molecules,2017,22(5):699.
[17] YANG S,WEI K,JIA F,et al.Characterization and Biological Activity of Taishan Pinus massoniana Pollen Polysaccharide In Vitro[J].Plos One,2015,10(3):e0115638.
[18] 周剑,苏德春,宋国权.白术多糖对H22肝癌小鼠抗肿瘤作用实验研究[J].亚太传统医药,2015,11(17):9-10.
[19] ZHA X Q,ZHAO H W,BANSAL V,et al.Immunoregulatory activities of Dendrobium huoshanense,polysaccharides in mouse intestine,spleen and liver[J].International Journal of Biological Macromolecules,2014,64(2):377-382.
[20] 王红,赵岩,蔡恩博,等.榆耳酸溶性多糖对肝癌H22小鼠的抑瘤作用研究[J].世界科学技术—中医药现代化,2017,19(1):142-148.
[21] 葛唯佳,赵岩,图力古尔,等.杂色云芝酸溶性多糖对H-(22)荷瘤小鼠的抗肿瘤作用[J].西北农林科技大学学报(自然科学版),2018(1):9-13.
[22] 魏静,李金凤,温成平,等.桑黄多糖对肝癌H22荷瘤小鼠的治疗作用[J].中药材,2016,39(12):2868-2870.
[23] LIN J,CHANG Y J,YANG W B,et al.The multifaceted effects of polysaccharides isolated from Dendrobium huoshanense on immune functions with the induction of interleukin-1 receptor antagonist (IL-1ra) in monocytes[J].Plos One,2014,9(4):e94040.