基于G1-熵权法和正交设计多指标优化刺五加提取工艺
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摘要
目的:基于正交设计结合G1-熵权法优选出刺五加有效成分的提取工艺。方法:采用G1-熵权法的主客观赋权法结合L9(34)正交设计,以料液比、提取时间、溶媒量为考察因素,刺五加B、刺五加苷E、异嗪皮啶、绿原酸的成分含量为评价指标,优选刺五加的最佳的提取工艺。结果:最佳提取工艺为提取时间75 min,料液比1∶25,70%的甲醇。在此条件下,4个评价指标的RSD均小于2. 0%,3批验证试验数据组间均无明显差异。结论:优选出的提取工艺方法稳定,刺五加B、刺五加苷E、异嗪皮啶和绿原酸的提取率较高,可为中药材刺五加现代工业化生产提供技术参考。
Objective: To optimize the extraction process of Acanthopanax senticosus ingredients based on orthogonal design combined with G1-entropy weight method. Methods: With the Syringin,Acanthopanax E,and Isofraxidin,Chlorogenic acid as evaluation index,and the ratio of material to liquid,extraction time and amount of solvent as investigate factors,the extraction technology of Acanthopanax senticosus was optimized by combination empowerment method based on G1-entropy method and L9(34) orthogonal design. Results: The optimal extraction process was extraction time 75 min,ratio of material to liquid 1∶ 25,70% methanol. Under these conditions,the RSD of the 4 evaluation indicators were less than 2. 0%,and there was no significant difference among the data groups of 3 batches of verification tests. Conclusion: The preferred extraction process is stable,and the extraction rate of Syringin,Acanthopanax E,Isofraxidin and Chlorogenic acid is high,which could provide technical references for modern industrial production of Acanthopanax senticosus.
Objective: To optimize the extraction process of Acanthopanax senticosus ingredients based on orthogonal design combined with G1-entropy weight method. Methods: With the Syringin,Acanthopanax E,and Isofraxidin,Chlorogenic acid as evaluation index,and the ratio of material to liquid,extraction time and amount of solvent as investigate factors,the extraction technology of Acanthopanax senticosus was optimized by combination empowerment method based on G1-entropy method and L9(34) orthogonal design. Results: The optimal extraction process was extraction time 75 min,ratio of material to liquid 1∶ 25,70% methanol. Under these conditions,the RSD of the 4 evaluation indicators were less than 2. 0%,and there was no significant difference among the data groups of 3 batches of verification tests. Conclusion: The preferred extraction process is stable,and the extraction rate of Syringin,Acanthopanax E,Isofraxidin and Chlorogenic acid is high,which could provide technical references for modern industrial production of Acanthopanax senticosus.
引文
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[15]王继龙,魏舒畅,刘永琦,等.基于G1-熵权法和正交设计优选黄芪百合颗粒的提取纯化工艺[J].中草药,2018,49(3):596-603.
[16]张慧,刘永强,汪小进,等.基于熵组合权重的调水工程项目管理模式选择[J].南水北调与水利科技,2015,13(6):1207-1211.
[17]仲怿,朱捷强,范骁辉,等.基于提取效率和成本控制的红参提取工艺多目标优化研究[J].中国中药杂志,2014,39(13):2495-2497.
[18]胡长明,黄露,许明旺,等.基于层次分析权数法的参葛降脂颗粒提取工艺优选[J].中国医院药学杂志,2015,35(24):2204-2208.
[19]李四海,高建德,陈建国,等.熵权法优选湖北海棠叶中总黄酮提取工艺[J].中国实验方剂学杂志,2012,18(23):56-58.
[20]朱紫烨,田彦芳,张迁,等. BP神经网络结合熵权法优化甘草皂苷提取工艺[J].中成药,2017,39(9):1830-1834.
[21]郭子雪,齐美然,李杨.基于G1法的河北省城市物流业竞争力评价研究[J].数学的实践与认识,2017,47(18):39-48.
[22]常艳梅,陈义华.基于指数标度的G1法及其应用[J].重庆理工大学学报(自然科学),2013,27(8):131-134.
[23]邓红雷,戴栋,李述文.基于层次分析-熵权组合法的架空输电线路综合运行风险评估[J].电力系统保护与控制,2017,45(1):28-34.
[24]李锐华,孙增涛,王永香,等.运用AHP结合熵权法优化疏风定喘颗粒炙麻黄组的醇沉工艺[J].中国实验方剂学杂志,2016,22(20):1-5.
[2]中国科学院植物志委员会.中国植物志:第五十四卷[M].北京:科学出版社,1978:99.
[3]董文婷,霍金海,张海燕,等.刺五加叶的药理作用研究进展[J].中国实验方剂学杂志,2015,21(23):220-223.
[4]田彦芳,万海同,朱紫烨,等.基于熵权法的多目标筛选甘草黄酮类成分纯化工艺[J].中草药,2016,47(7):1118-1125.
[5]张崇禧,张莹莹,田芯,等. HPLC测定不同产地刺五加中紫丁香苷的含量[J].中成药,2008,30(11):1648-1651.
[6]杨敬芝,李建北,张东明,等. HPLC法测定不同产地的刺五加中刺五加苷B和E含量[J].中药材,2005,28(8):669-670.
[7]张萍. HPLC测定不同产地、不同生长年限刺五加药材中异嗪皮啶[J].药物分析杂志,2010,30(12):2433-2435.
[8]任晋,廖娴,谢镇山,等. HPLC法测定刺五加、五加皮与红毛五加皮中3种成分的含量[J].广东药学院学报,2015,31(5):602-606.
[9]王晓美,刘春艳,吴小娟,等.基于多目标遗传算法优化刺五加根提取工艺的研究[J].中国药物与临床,2013,13(7):825-828.
[10]席利莎,木泰华,孙红男.绿原酸类物质的国内外研究进展[J].核农学报,2014,28(2):292-301.
[11]王玲娜,姚佳欢,马超美.绿原酸的研究进展[J].食品与生物技术学报,2017,36(11):1121-1130.
[12]叶利,田晓霞,尚凡,等.基于G1-熵权法的供电服务满意度指标权重组合优化方法[J].湖北电力,2016,40(9):45-50.
[13]陈端,曹阳,夏辉,等.基于熵权法和G1法的大坝监测指标权重融合[J].水电能源科学,2012,30(6):92-94.
[14]张俸溥.基于G1-熵权法的商业银行绿色信贷实施效果评价研究[D].北京:首都经济贸易大学,2017.
[15]王继龙,魏舒畅,刘永琦,等.基于G1-熵权法和正交设计优选黄芪百合颗粒的提取纯化工艺[J].中草药,2018,49(3):596-603.
[16]张慧,刘永强,汪小进,等.基于熵组合权重的调水工程项目管理模式选择[J].南水北调与水利科技,2015,13(6):1207-1211.
[17]仲怿,朱捷强,范骁辉,等.基于提取效率和成本控制的红参提取工艺多目标优化研究[J].中国中药杂志,2014,39(13):2495-2497.
[18]胡长明,黄露,许明旺,等.基于层次分析权数法的参葛降脂颗粒提取工艺优选[J].中国医院药学杂志,2015,35(24):2204-2208.
[19]李四海,高建德,陈建国,等.熵权法优选湖北海棠叶中总黄酮提取工艺[J].中国实验方剂学杂志,2012,18(23):56-58.
[20]朱紫烨,田彦芳,张迁,等. BP神经网络结合熵权法优化甘草皂苷提取工艺[J].中成药,2017,39(9):1830-1834.
[21]郭子雪,齐美然,李杨.基于G1法的河北省城市物流业竞争力评价研究[J].数学的实践与认识,2017,47(18):39-48.
[22]常艳梅,陈义华.基于指数标度的G1法及其应用[J].重庆理工大学学报(自然科学),2013,27(8):131-134.
[23]邓红雷,戴栋,李述文.基于层次分析-熵权组合法的架空输电线路综合运行风险评估[J].电力系统保护与控制,2017,45(1):28-34.
[24]李锐华,孙增涛,王永香,等.运用AHP结合熵权法优化疏风定喘颗粒炙麻黄组的醇沉工艺[J].中国实验方剂学杂志,2016,22(20):1-5.