澳洲茶树离体培养器官发生的形态解剖学观察
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摘要
采用石蜡切片和光学显微技术对澳洲茶树(Melaleuca alternifolia)组培苗离体器官发生进行形态解剖学观察。结果表明:在选定的培养基上,澳洲茶树芽的增殖直接由器官诱导形成,有效地保持了其遗传的稳定性。在诱导生根前,没有潜在的根原基,说明其生根类型为诱导生根型;不定根发生形式属皮部生根类型;但生根过程中诱导产生愈伤组织,其对根原基的形成有一定刺激作用,因此,诱导出适当的愈伤组织,对澳洲茶树不定根的生成具有重要的作用。
The morphological and anatomical observation on the organogenesis from tissue-culture plantlets of Melaleuca alternifolia was carried out with the method of paraffin section and optical microscopy technique. The results were obtained as follows: in the selected culture medium,the multiplied buds of M. alternifolia formed directly through the organogenesis way,thus the genetic stability of this plant could be effectively maintained. There was no potential root primordium before the rooting was induced,suggesting that its rooting type belonged to induced type. The type of adventitious root organogenesis belonged to cortexrooting type. But callus was induced out during the rooting,which could stimulate the formation of root primordium. Therefore,inducing proper quantity of callus played an important role in the formation of adventitious roots of M. alternifolia in vitro culture.
The morphological and anatomical observation on the organogenesis from tissue-culture plantlets of Melaleuca alternifolia was carried out with the method of paraffin section and optical microscopy technique. The results were obtained as follows: in the selected culture medium,the multiplied buds of M. alternifolia formed directly through the organogenesis way,thus the genetic stability of this plant could be effectively maintained. There was no potential root primordium before the rooting was induced,suggesting that its rooting type belonged to induced type. The type of adventitious root organogenesis belonged to cortexrooting type. But callus was induced out during the rooting,which could stimulate the formation of root primordium. Therefore,inducing proper quantity of callus played an important role in the formation of adventitious roots of M. alternifolia in vitro culture.
引文
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[25]江玲,管晓春.植物激素与不定根的形成[J].生物学通报,2000,35(11):17-19.
[26]王涛.植物扦插繁殖技术[M].北京:科学技术出版社,1989.
[2]居解语,何立平.互叶白千层精油化学成分差异的研究[J].经济林研究,1999,17(2):6-7.
[3]李恒树,黄耀恒.互叶白千层的栽培技术[J].广西林业科学,2007,36(3):156-157.
[4]刘海龙,覃子海,张烨,等.配方施肥对澳洲茶树枝叶产量和产油量的影响[J].热带农业科学,2017,37(9):21-25.
[5]高楠.互叶白千层扦插育苗技术初探[J].福建林业科技,2010,37(3):67-69,98.
[6]梁忠云,李桂珍,陈海燕,等.白千层芳香油树种的引进研究进展[J].香料香精化妆品,2011(6):37-40.
[7]肖玉菲,刘海龙,刘雄盛,等.澳洲茶树组培继代芽瓶外生根技术研究[J].西部林业科学,2018,47(2):86-90.
[8]刘海龙,陈博雯,肖玉菲,等.澳洲茶树组培继代增殖技术优化[J].西部林业科学,2017,46(5):23-28.
[9]李桂珍,梁忠云,常新民,等.4-松油醇型白千层枝叶储存过程中精油的变化[J].广西林业科学,2014,43(2):204-206.
[10]覃子海,肖玉菲,唐复呈,等.互叶白千层扦插繁殖技术[J].广西林业科学,2017,46(1):111-114.
[11]陈博雯,袁剑英,覃子海,等.4-松油醇型澳洲茶树组培生根技术研究[J].西北林学院学报,2017,32(4):120-123,208.
[12]吴丽君,陈达,高楠,等.高含油互叶白千层高效栽培配套技术研究[J].福建农业学报,2017,32(7):734-738.
[13]张烨,覃子海,肖玉菲,等.配方施肥对澳洲茶树幼林生长性状的影响研究[J].西部林业科学,2018,47(1):29-33.
[14]李正理.植物制片技术[M].北京:科学出版社,1987.
[15]薛银芳,赵大球,周春华,等.芍药组织培养的研究进展[J].北方园艺,2012(4):167-170.
[16]张亚锋,曹家树,武涛.南瓜属植物再生体系的建立及其应用[J].植物生理学通讯,2007,43(3):599-604.
[17]金万梅,董静,王媛花,等.葡萄器官发生途径再生不定芽的遗传稳定性[J].分子植物育种,2009,7(2):375-379.
[18]王鹏良,吴幼媚,蔡玲,等.油茶岑软3号组培苗遗传稳定性SRAP分析[J].林业科技开发,2012,26(6):17-19.
[19]秦丽凤.杉木优良无性系组培技术体系的建立[J].南方农业学报,2018,49(6):1183-1188.
[20]郭素娟.林木扦插生根的解剖学及生理学研究进展[J].北京林业大学学报,1997,19(4):64-69.
[21]扈顺.四合木繁殖特性及扦插生根解剖学研究[D].呼和浩特:内蒙古农业大学,2014.
[22]丘醒球,余倩珠,张少翃.桉树插条生根机理解剖研究初报[J].林业科学研究,1995,8(2):170-176.
[23]涂翠琴,江洪茹,涂艺声.猕猴桃全光喷雾扦插快速育苗研究[J].果树科学,1991(1):25-30.
[24]宋晓琛,于芬,戴小英,等.油茶组培苗生根的解剖学机理研究[J].中国农学通报,2013,29(34):7-11.
[25]江玲,管晓春.植物激素与不定根的形成[J].生物学通报,2000,35(11):17-19.
[26]王涛.植物扦插繁殖技术[M].北京:科学技术出版社,1989.