信号分子OHHL对厌氧同步脱氮除硫的影响
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摘要
为了探究信号分子对厌氧同步脱氮除硫的影响,本文采用信号分子OHHL(N-3-oxo-hexanoyl-homoserine-lactone),在两种进水硫化物浓度(100 mg/L和200 mg/L)条件下,研究OHHL浓度对脱氮除硫的影响.结果表明,在进水硫化物浓度为100 mg/L条件下,信号分子OHHL投加浓度为1.5μM时硫化物和硝酸盐在反应进行36 h时的去除率高达99.0%、96.1%,单质硫产量高达60 mg/L,远高于未投加信号分子及投加浓度为1.0μM、2.0μM的体系.可见投加信号分子可以促进硫化物和硝酸盐的去除,提高单质硫产量.这是由于投加信号分子时(1.5μM)体系中的总菌数量大幅度提高至1.1×10~5 CFU/mL,远高于其他条件下的相应值(2.6×10~4～7.0×10~4 CFU/mL),增强了异养反硝化作用.此外,进水硫化物浓度为200 mg/L时,需要延长反应时间至72h才可达到较高(91.7%)的硝酸盐去除效果.
The subject of this study is to investigate the effect of signal molecules on anaerobic simultaneous denitrification and desulfurization. Four concentrations(0 μM, 1.0 μM, 1.5 μM and 2.0 μM) of N-3-oxo-hexanoyl-homoserine-lactone(OHHL) were applied to explore the effect of OHHL concentration on simultaneous removal of nitrate and sulfide with 100 mg/L or 200 mg/L of sulfide influent. The results suggested that when the influent sulfide concentration was 100 mg/L, sulfide and nitrate removal efficiencies reached 99.0% and 96.1% with the application of 1.5 μM OHHL after 36 h reaction. Meanwhile, the yield of elemental sulfur was as high as 60 mg/L, much higher than that with 0 μM, 1.0 μM and 2.0 μM OHHL amended. The above results indicated that the removal of sulfide and nitrate and the yield of elemental sulfur were enhanced with the application of OHHL. The possible explanation was that with the addition of 1.5 μM OHHL, the total population of bacteria substantially increased to 1.1 × 10~5 CFU/mL, which was significantly higher than the corresponding values(2.6 ×10~4 ～7.0 × 10~4 CFU/mL) under the other conditions, thus leading to an enhancement of the heterotrophic denitrification. Moreover, as the influent sulfide concentration was increased to 200 mg/L, the extent of reaction time(72 h) was necessary to obtain a relatively high nitrate removal efficiency(91.7%).
The subject of this study is to investigate the effect of signal molecules on anaerobic simultaneous denitrification and desulfurization. Four concentrations(0 μM, 1.0 μM, 1.5 μM and 2.0 μM) of N-3-oxo-hexanoyl-homoserine-lactone(OHHL) were applied to explore the effect of OHHL concentration on simultaneous removal of nitrate and sulfide with 100 mg/L or 200 mg/L of sulfide influent. The results suggested that when the influent sulfide concentration was 100 mg/L, sulfide and nitrate removal efficiencies reached 99.0% and 96.1% with the application of 1.5 μM OHHL after 36 h reaction. Meanwhile, the yield of elemental sulfur was as high as 60 mg/L, much higher than that with 0 μM, 1.0 μM and 2.0 μM OHHL amended. The above results indicated that the removal of sulfide and nitrate and the yield of elemental sulfur were enhanced with the application of OHHL. The possible explanation was that with the addition of 1.5 μM OHHL, the total population of bacteria substantially increased to 1.1 × 10~5 CFU/mL, which was significantly higher than the corresponding values(2.6 ×10~4 ～7.0 × 10~4 CFU/mL) under the other conditions, thus leading to an enhancement of the heterotrophic denitrification. Moreover, as the influent sulfide concentration was increased to 200 mg/L, the extent of reaction time(72 h) was necessary to obtain a relatively high nitrate removal efficiency(91.7%).
引文
[1] 魏世林.制革废水中的硫化物对环境的污染及其治理方法[J].中国皮革,2003,32(1):3-5.WEI Shilin.Environmental pollution and treatment method of sulfides in tannery effluents[J].China Leather,2003,32(1):3-5.
[2] WANG A,LIU C,REN N,et al.Simultaneous removal of sulfide,nitrate and acetate:Kinetic modeling.[J].Journal of Hazardous Materials,2010,178(1-3):35.
[3] YIN Z,LI X,QI Z.Effects of sulfide on the integration of denitrification with anaerobic digestion[J].Journal of Bioscience & Bioengineering,2015,120(4):426-431.
[4] 蔡靖,郑平,胡宝兰,等.硫氮比对厌氧生物同步脱氮除硫工艺性能的影响[J].环境科学学报,2008,28(8):1506-1514.CAI Jing,ZHENG Ping,HU Baolan,et al.The effect of sulfide to nitrate ratio on the performance of a simultaneous anaerobic sulfide and nitrate removal process[J].Acta Scientiae Circumstantiae,2008,28(8):1506 -1514.
[5] 陈婷婷,肖蓉蓉,施金豆,等.影响AFB-MFC同步脱氮除硫性能因素的研究[J].环境科学与技术,2013(10):85-89.CHEN Tingting,XIAO Rongrong,SHI Jindou,et al.Factors affecting performance of AFB-MFC for simultaneous nitrogen and sulfate removal[J].Environmental Science & Technology,2013,36(10):85-89.
[6] 冯艳.人体内细胞间的信号转导[J].生物学教学.2010,35(8):68-69.FEN Yan.Signal transduction between cells in human body[J].Biology teaching,2010,35(8):68-69.
[7] 侯保连,李安婕,孙趣.AHLs群体感应信号分子对硝化污泥附着生长及硝化效果的影响[J].环境科学学报,2015,35(9):2773-2779.HOU Baolan,LI Anjie,SUN Q.The effect of N-acy-homoserine lactones mediated quorum-sensing on the adhesion growth and nitrification nitrifying sludge[J].Acta Scientiae Circumstantiae,2015,35(9):2773-2779.
[8] 肖琳,杨柳燕,蒋丽娟,等.附生假单胞菌信号分子对铜绿微囊藻磷代谢的影响[J].环境科学学报,2005,25(4):557-561.XIAO Lin,YANG Liuyan,JIANG Lijuan,et al.The effect of signal molecular produced by attached bacteria on the Mi-crosystis aeruginosa phosphorus metabolism[J].Acta Scientiae Circumstantiae,2005,25(4):557 -561.
[9] KOENIG A,LIU L H.Kinetic model of autotrophic denitrification in sulphur packed-bed reactors[J].Water Research,2001,35(8):1969-1978.
[10] STEUDEL R.Inorganic Polysulfides S,n,2-,and Radical Anions S,n,·-[M]// Elemental Sulfur und Sulfur-Rich Compounds II.Berlin Heidelberg:Springer,2003:281-288.
[11] YANG W,VOLLERTSEN J,Hvitved-Jacobsen T.Anoxic sulfide oxidation in wastewater of sewer networks[J].Water Science & Technology A Journal of the International Association on Water Pollution Research,2005,52(3):191.
[12] 徐金兰,侯圣春,黄廷林.强化厌氧污泥体系同步脱硫反硝化特性研究[J].环境科学,2010,31(5):1246-1251.XU Jinlan,HOU Shengchun,HUANG Tinglin.Characteristic of autotrophic denitrification in bioaugmented anaerobic system[J].Environmental Science,2010,31(5):1246-1251.
[13] XU J,DING K,YANG C,et al.Regulation of influent sulfide concentration on anaerobic denitrifying sulfide removal.[J].Environmental Technology,2017:1-21.
[14] AMANN R I.In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes[M]//Molecular Microbial Ecology Manual.Switzerland:Springer,1995:331-345.
[15] DAIMS H,LüCKEr S,WAGNER M.Daime,a novel image analysis program for microbial ecology and biofilm research[J].Environmental Microbiology,2006,8(2):200-213.
[16] CHEN C,REN N,WANG A,et al.Microbial community of granules in expanded granular sludge bed reactor for simultaneous biological removal of sulfate,nitrate and lactate[J].Applied Microbiology & Biotechnology,2008,79(6):1071-1077.
[17] 李军,张文文,王立军,等.硫自养反硝化同步脱氮除硫启动试验[J].沈阳建筑大学学报(自然科学版),2010,26(1):162-165.LI Jun,ZHANG Wenwen,WANG Lijun,et al.Start-up test of simultaneous nitrogen and sulfur removal by sulfur autotrophic denitrification[J].Journal of Shenyang Jianzhu University (Natural Science),2010,26(1):162-165.
[18] 郑香凤,汪莉,郑天龙,等.生物填料塔同步脱氮除硫的研究[J].环境工程,2013(s1):433-436.ZHENG Xiangfeng,WANG Li,ZHENG Tianlong,et al.Study of Simultaneous Denitrification and Desulfurization by Biological Packed Tower[J].Environmental Engineering,2013(s1):433-436.
[19] WANG A J,DU D Z,REN N Q,et al.Tentative study on a new way of simultaneous desulfurization and denitrification[J].Chinese Journal of Chemical Engineering,2005,13(3):422-425.
[20] 李巍,赵庆良,刘颢.缺氧附着生长反应器同步脱氮除硫除碳运行效果探讨[J].环境科学,2008,29(7):1855-1859.LI Wei,ZHAO Qingliang,LIU Jing.Simultaneous removal of sulfide,nitrogenous compounds and organic carbon by anoxic attached-growth bioreactor[J].Environmental Science,29(7):1855-1859.
[2] WANG A,LIU C,REN N,et al.Simultaneous removal of sulfide,nitrate and acetate:Kinetic modeling.[J].Journal of Hazardous Materials,2010,178(1-3):35.
[3] YIN Z,LI X,QI Z.Effects of sulfide on the integration of denitrification with anaerobic digestion[J].Journal of Bioscience & Bioengineering,2015,120(4):426-431.
[4] 蔡靖,郑平,胡宝兰,等.硫氮比对厌氧生物同步脱氮除硫工艺性能的影响[J].环境科学学报,2008,28(8):1506-1514.CAI Jing,ZHENG Ping,HU Baolan,et al.The effect of sulfide to nitrate ratio on the performance of a simultaneous anaerobic sulfide and nitrate removal process[J].Acta Scientiae Circumstantiae,2008,28(8):1506 -1514.
[5] 陈婷婷,肖蓉蓉,施金豆,等.影响AFB-MFC同步脱氮除硫性能因素的研究[J].环境科学与技术,2013(10):85-89.CHEN Tingting,XIAO Rongrong,SHI Jindou,et al.Factors affecting performance of AFB-MFC for simultaneous nitrogen and sulfate removal[J].Environmental Science & Technology,2013,36(10):85-89.
[6] 冯艳.人体内细胞间的信号转导[J].生物学教学.2010,35(8):68-69.FEN Yan.Signal transduction between cells in human body[J].Biology teaching,2010,35(8):68-69.
[7] 侯保连,李安婕,孙趣.AHLs群体感应信号分子对硝化污泥附着生长及硝化效果的影响[J].环境科学学报,2015,35(9):2773-2779.HOU Baolan,LI Anjie,SUN Q.The effect of N-acy-homoserine lactones mediated quorum-sensing on the adhesion growth and nitrification nitrifying sludge[J].Acta Scientiae Circumstantiae,2015,35(9):2773-2779.
[8] 肖琳,杨柳燕,蒋丽娟,等.附生假单胞菌信号分子对铜绿微囊藻磷代谢的影响[J].环境科学学报,2005,25(4):557-561.XIAO Lin,YANG Liuyan,JIANG Lijuan,et al.The effect of signal molecular produced by attached bacteria on the Mi-crosystis aeruginosa phosphorus metabolism[J].Acta Scientiae Circumstantiae,2005,25(4):557 -561.
[9] KOENIG A,LIU L H.Kinetic model of autotrophic denitrification in sulphur packed-bed reactors[J].Water Research,2001,35(8):1969-1978.
[10] STEUDEL R.Inorganic Polysulfides S,n,2-,and Radical Anions S,n,·-[M]// Elemental Sulfur und Sulfur-Rich Compounds II.Berlin Heidelberg:Springer,2003:281-288.
[11] YANG W,VOLLERTSEN J,Hvitved-Jacobsen T.Anoxic sulfide oxidation in wastewater of sewer networks[J].Water Science & Technology A Journal of the International Association on Water Pollution Research,2005,52(3):191.
[12] 徐金兰,侯圣春,黄廷林.强化厌氧污泥体系同步脱硫反硝化特性研究[J].环境科学,2010,31(5):1246-1251.XU Jinlan,HOU Shengchun,HUANG Tinglin.Characteristic of autotrophic denitrification in bioaugmented anaerobic system[J].Environmental Science,2010,31(5):1246-1251.
[13] XU J,DING K,YANG C,et al.Regulation of influent sulfide concentration on anaerobic denitrifying sulfide removal.[J].Environmental Technology,2017:1-21.
[14] AMANN R I.In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes[M]//Molecular Microbial Ecology Manual.Switzerland:Springer,1995:331-345.
[15] DAIMS H,LüCKEr S,WAGNER M.Daime,a novel image analysis program for microbial ecology and biofilm research[J].Environmental Microbiology,2006,8(2):200-213.
[16] CHEN C,REN N,WANG A,et al.Microbial community of granules in expanded granular sludge bed reactor for simultaneous biological removal of sulfate,nitrate and lactate[J].Applied Microbiology & Biotechnology,2008,79(6):1071-1077.
[17] 李军,张文文,王立军,等.硫自养反硝化同步脱氮除硫启动试验[J].沈阳建筑大学学报(自然科学版),2010,26(1):162-165.LI Jun,ZHANG Wenwen,WANG Lijun,et al.Start-up test of simultaneous nitrogen and sulfur removal by sulfur autotrophic denitrification[J].Journal of Shenyang Jianzhu University (Natural Science),2010,26(1):162-165.
[18] 郑香凤,汪莉,郑天龙,等.生物填料塔同步脱氮除硫的研究[J].环境工程,2013(s1):433-436.ZHENG Xiangfeng,WANG Li,ZHENG Tianlong,et al.Study of Simultaneous Denitrification and Desulfurization by Biological Packed Tower[J].Environmental Engineering,2013(s1):433-436.
[19] WANG A J,DU D Z,REN N Q,et al.Tentative study on a new way of simultaneous desulfurization and denitrification[J].Chinese Journal of Chemical Engineering,2005,13(3):422-425.
[20] 李巍,赵庆良,刘颢.缺氧附着生长反应器同步脱氮除硫除碳运行效果探讨[J].环境科学,2008,29(7):1855-1859.LI Wei,ZHAO Qingliang,LIU Jing.Simultaneous removal of sulfide,nitrogenous compounds and organic carbon by anoxic attached-growth bioreactor[J].Environmental Science,29(7):1855-1859.