微压气升循环流反应器与完全混合式反应器运行效果及微生物种群结构比较
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摘要
微压气升循环流反应器(MP-ALR)是在完全混合式反应器(CMR)基础上开发的一种新型污水处理装置。对比研究了MP-ALR和CMR对COD和氨氮的去除效果,并分析了微生物的种群变化和相似性。结果表明,MP-ALR对COD与氨氮的去除效果比CMR更强,抗有机负荷冲击的能力也更强,更适合高浓度有机工业废水的处理。变性梯度凝胶电泳(DGGE)分析表明,MP-ALR和CMR微生物种群结构差异较大,第91、103、116、124个周期末,MP-ALR内的微生物种群数均小于CMR。进一步聚类分析表明,MP-ALR和CMR内的种群均呈现出了逐渐演替的过程,且MP-ALR的演替程度大于CMR。
Micro-pressure air-lift loop reactor(MP-ALR)is a new wastewater treatment device developed from completely mixed reactor(CMR).COD and ammonia nitrogen removal efficiency,variation of microbial population and similarity were studied between MP-ALR and CMR.Results indicated that the removal efficiencies of COD and ammonia nitrogen of MP-ALR were higher than that of CMR.MP-ALR showed better ability to resist organic load,too.The results of denaturation gradient gel electrophoresis(DGGE)showed that the microbial population in MP-ALR was much lower than that in CMR with significant difference at the end of Cycles 91,103,116 and 124.The cluster analysis further confirmed that the microbial population in MP-ALR and CMR had a gradually succession,repectively.The succession in MP-ALR was greater than that in CMR.
Micro-pressure air-lift loop reactor(MP-ALR)is a new wastewater treatment device developed from completely mixed reactor(CMR).COD and ammonia nitrogen removal efficiency,variation of microbial population and similarity were studied between MP-ALR and CMR.Results indicated that the removal efficiencies of COD and ammonia nitrogen of MP-ALR were higher than that of CMR.MP-ALR showed better ability to resist organic load,too.The results of denaturation gradient gel electrophoresis(DGGE)showed that the microbial population in MP-ALR was much lower than that in CMR with significant difference at the end of Cycles 91,103,116 and 124.The cluster analysis further confirmed that the microbial population in MP-ALR and CMR had a gradually succession,repectively.The succession in MP-ALR was greater than that in CMR.
引文
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[2]万立国,边德军,艾胜书,等.多菌群污水生物处理反应器中污泥分布的试验研究[J].环境污染与防治,2012,34(2):18-21.
[3]BIAN D J,REN Q K,WAN L G,et al.Effects of aeration volume on velocity distribution of fluid in multi-flora biological wastewater treatment reactor[J].Advanced Materials Research,2011,255:2740-2744.
[4]BOUALLAGUI H M,TORRIJOS J J,GODON R,et al.Microbial monitoring by molecular tools of a two-phase anaerobic bioreactor treating fruit and vegetable wastes[J].Biotechnology Letters,2004,26(10):857-862.
[5]BUZZINI A P,SAKAMOTO I K,VARESCHE M B,et al.Evaluation of the microbial diversity in an UASB reactor treating wastewater from an unbleached pulp plant[J].Process Biochemistry,2006,41(1):168-176.
[6]KONGR Y C,LEES K Y,LAW T W F,et al.Rapid detection of six types of bacterial pathogens in marine waters by multiplex PCR[J].Water Research,2002,36(11):2802-2812.
[7]LAGALLY E T,SCHERDR J R,BLAZEJ R G,et al.Integrated portable genetic analysis microsystem for pathogenic/infectious disease detection[J].Analytical Chemistry,2004,76(11):3162-3170.
[8]LAPARA T M,NAKATSU C H,PANTEA L M,et al.Stability of the bacterial communities supported by a seven-stage biological process treating pharmaceutical wastewater as revealed by PCR-DGGE[J].Water Research,2002,36(3):638-646.
[9]宋志伟,程晓霞,乔艳云,等.接种污泥对好氧颗粒污泥稳定性的影响[J].吉林大学学报(地球科学版),2011,41(3):873-878.
[10]MIURA Y,HIRAIWA M N,ITONAGA T,et al.Bacterial community structures in MBRs treating municipal wastewater:relationship between community stability and reactor performance[J].Water Research,2007,41(3):627-637.
[11]于凤庆,孙宝盛,陈谊,等.MBR活性污泥培养驯化过程中生物多样性研究[J].环境科学学报,2012,32(9):2084-2090.
[12]YU Z,GARCIA R,SCHANBACHER R,et al.Evaluations of different hypervariable regions of archaeal 16SrRNA genes in profiling of methanogens by archaea-specific PCR and denaturing gradient gel electrophoresis[J].Applied and Environmental Microbiology,2008,74(3):889-893.
[13]FERNANDEZ A S,HASHSHAM S A,DOLLHOPF S L,et al.Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose[J].Applied and Environmental Microbiology,2000,66(9):4058-4067.
[14]国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[15]GERARD M,WAAL E C,VITTERLINDEN A G.Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes encoding for 16SrRNA[J].Applied and Environmental Microbiology,1993,59(3):695-700.
[16]MUYZER G,BRINKHOFF T,NUBEL U,et al.Molecular microbial ecology manual[M].Dordrecht:Kluwer Academic Publishers,1996.
[17]孙宝盛,张斌,吴卿,等.应用PCR-DGGE技术解析MBR中微生物群落多样性[J].天津大学学报,2008,41(3):356-361.
[18]杨培,王淑莹,顾升波,等.中试SBR长期运行中粘性膨胀现象及原因分析[J].环境科学学报,2010,30(7):1384-1389.
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