利用甘薯淀粉生产废水培养侧孢短芽孢杆菌的研究
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摘要
为探明利用甘薯淀粉生产废水培养侧孢短芽孢杆菌(Brevibacillus laterosporus)的最适条件,采用单因素试验,研究了甘薯淀粉废水浓度、初始p H值、培养温度对侧孢短芽孢杆菌菌体生长的影响。结果表明:甘薯淀粉废水浓度40%,初始p H值为7.0~8.0,培养温度为30~35°C,以2%比例接种侧孢短芽孢杆菌种子液,150r·min-1培养24h时,菌体浓度最高可达1.63×109cfu·m L-1。在此基础上,应用Placket-Burman设计,对微生物培养基中常用的8种无机盐离子(KH2PO4,Mg SO4,Ca Cl2,Na Cl,Fe SO4,Cu SO4,Mn SO4和Zn SO4)进行了评价。结果表明:向甘薯淀粉废水中添加不同种类或不同浓度的外源无机盐离子,对菌体生长的影响不显著。试验结果初步证明了利用甘薯淀粉生产废水发酵培养侧孢短芽孢杆菌的可行性,为甘薯淀粉生产废水的资源化利用和低成本生产侧孢短芽孢杆菌提供了一条可参考的技术路线。
This experiment was conducted to explore the optimum culture conditions of Brevibacillus laterosporus using sweet potato wastewater. The effects of concentration, initial p H and temperature on the production of B. laterosporus were investigated by single-factor experiments. With SPSW diluted to 40%, initial p H 7.0-8.0, temperature 30-35°C, inoculum 2%, rotate speed at150 r·min-1, a maximum biomass of 1.63×109cfu·m L-1was achieved after 24 h cultivation. The two-level Placket-Burman(PB)design was used to explore the effects of mineral salts(KH2PO4, Mg SO4, Ca Cl2, Na Cl, Fe SO4, Cu SO4, Mn SO4, Zn SO4) that generally used as components of microbial medium. The effects on the production of B. laterosporus with different kinds or concentrations of mineral salts added to SPSW were not significant. Our experiments proved the feasibility to culture B.laterosporus with SPSW as low-cost medium, and provided an environmental friendly way to utilize SPSW.
This experiment was conducted to explore the optimum culture conditions of Brevibacillus laterosporus using sweet potato wastewater. The effects of concentration, initial p H and temperature on the production of B. laterosporus were investigated by single-factor experiments. With SPSW diluted to 40%, initial p H 7.0-8.0, temperature 30-35°C, inoculum 2%, rotate speed at150 r·min-1, a maximum biomass of 1.63×109cfu·m L-1was achieved after 24 h cultivation. The two-level Placket-Burman(PB)design was used to explore the effects of mineral salts(KH2PO4, Mg SO4, Ca Cl2, Na Cl, Fe SO4, Cu SO4, Mn SO4, Zn SO4) that generally used as components of microbial medium. The effects on the production of B. laterosporus with different kinds or concentrations of mineral salts added to SPSW were not significant. Our experiments proved the feasibility to culture B.laterosporus with SPSW as low-cost medium, and provided an environmental friendly way to utilize SPSW.
引文
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[12]LANG W,SIRISANSANEEYAKUL S,NGIWSARA L,et al.Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR 1911:Taward dye decolorization using a packed-bed metal affinity reactor[J].Bioresource Technology,2013,150:298-306.
[13]殷永泉,单文坡,纪霞,等.淀粉废水处理方法综述[J].环境污染与防治,2006,27(8):625-629.
[14]CHANG M,ZHOU S G,LU N,et al.Starch processing wastewater as a new medium for production of Bacillus thuringiensis[J].World Journal of Microbiology and Biotechnology,2008,24(4):441-447.
[15]MUNIRAJ I K,XIAO L,HU Z,et al.Microbial lipid production from potato processing wastewater using oleaginous filamentous fungi Aspergillus oryzae[J].Water Research,2013,47(10):3477-3483.
[16]XU S,BAI Z,JIN B,et al.Bioconversion of wastewater from sweet potato starch production to Paenibacillus polymyxa biofertilizer for tea plants[J].Scientific Reports,2014,4:1-7.
[17]韩庆莉,赵志瑞,周永双,等.甘薯淀粉废水培养类球红细菌条件优化[J].安徽农学通报,2012,18(13):35-38.
[18]周玉,负建民,宋德龙.马铃薯淀粉废水SCP发酵菌种筛选及发酵条件优化[J].食品工业,2013,34(3):20-23.
[19]代志凯,张翠,阮征.试验设计和优化及其在发酵培养基优化中的应用[J].微生物学报,2010,37(6):894-903.
[2]SHIDA O,TAKAGI H,KADOWAKI K,et al.Proposal for two new genera,Brevibacillus gen.nov.and Aneurinibacillus gen.nov[J].International Journal of Systematic Bacteriology,1996,46(4):939-946.
[3]GARRITY G M,BELL J A,LILBURN G T.Taxonomic outline of the prokaryotes release 5.0 Bergey's mannual of systematic bacteriology,2ed edition[M].New York:Springer-Verla,2004.
[4]赵云彤.侧孢芽孢杆菌BL-1的诱变选育及发酵条件优化[D].哈尔滨:黑龙江大学,2009.
[5]PRASANNA L,EIJSINK V G H,MEADOW R,et al..A novel strain of Brevibacillus laterosporus produces chitinases that contribute to its biocontrol potential[J].Applied Microbiology and Biotechnology,2013,97(4):1601-1611.
[6]ZHAO J,GUO L,ZENG H,et al.Purification and characterization of a novel antimicrobial peptide from Brevibacillus laterosporu s strain A60[J].Peptides,2012,33(2):206-211.
[7]HUANG X,TIAN B,NIU Q,et al.An extracellular protease from Brevibacillus laterosporus G4 without parasporal crystals can serve as a pathogenic factor in infection of nematodes[J].Research in Microbiology,2005,156(5):719-727.
[8]KAKAR K U,NAWAZ Z,CUI Z,et al.Characterizing the mode of action of Brevibacillus laterosporus B4 for control of bacterial brown strip of rice caused by A.avenae subsp.avenae RS-1[J].World Journal of Microbiology and Biotechnology,2014,30(2):469-478.
[9]DE OLIVEIRA E J,RABINOVITCH L,MONNERAT R G,et al.Molecular characterization of Brevibacillus laterosporus and its potential use in biological control[J].Applied and Environmental Microbiology,2004,70(11):6657-6664.
[10]GOMARE S S,KALME S D,GOVINDWAR S P.Biodegradation of Navy Blue-3G by Brevibacillus laerosporus MTCC 2298[J].Acta Chimica Slovenica,2009,56(4):789-796.
[11]KURADE M B,WAGHMODE T R,KABRA A N,et al.Degradation of a xenobiotic textile dye,Disperse Brown 118,by Brevibacillus laterosporus[J].Biotechnology Letters,2013,35(10):1593-1598.
[12]LANG W,SIRISANSANEEYAKUL S,NGIWSARA L,et al.Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus TISTR 1911:Taward dye decolorization using a packed-bed metal affinity reactor[J].Bioresource Technology,2013,150:298-306.
[13]殷永泉,单文坡,纪霞,等.淀粉废水处理方法综述[J].环境污染与防治,2006,27(8):625-629.
[14]CHANG M,ZHOU S G,LU N,et al.Starch processing wastewater as a new medium for production of Bacillus thuringiensis[J].World Journal of Microbiology and Biotechnology,2008,24(4):441-447.
[15]MUNIRAJ I K,XIAO L,HU Z,et al.Microbial lipid production from potato processing wastewater using oleaginous filamentous fungi Aspergillus oryzae[J].Water Research,2013,47(10):3477-3483.
[16]XU S,BAI Z,JIN B,et al.Bioconversion of wastewater from sweet potato starch production to Paenibacillus polymyxa biofertilizer for tea plants[J].Scientific Reports,2014,4:1-7.
[17]韩庆莉,赵志瑞,周永双,等.甘薯淀粉废水培养类球红细菌条件优化[J].安徽农学通报,2012,18(13):35-38.
[18]周玉,负建民,宋德龙.马铃薯淀粉废水SCP发酵菌种筛选及发酵条件优化[J].食品工业,2013,34(3):20-23.
[19]代志凯,张翠,阮征.试验设计和优化及其在发酵培养基优化中的应用[J].微生物学报,2010,37(6):894-903.